+

WO2000015665A3 - A method of producing a functional immunoglobulin superfamily protein - Google Patents

A method of producing a functional immunoglobulin superfamily protein Download PDF

Info

Publication number
WO2000015665A3
WO2000015665A3 PCT/DK1999/000484 DK9900484W WO0015665A3 WO 2000015665 A3 WO2000015665 A3 WO 2000015665A3 DK 9900484 W DK9900484 W DK 9900484W WO 0015665 A3 WO0015665 A3 WO 0015665A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
functional
immunoglobulin superfamily
class
mhc class
Prior art date
Application number
PCT/DK1999/000484
Other languages
French (fr)
Other versions
WO2000015665A2 (en
Inventor
Lars Oestergaard Pedersen
Soeren Buus
Original Assignee
Lars Oestergaard Pedersen
Soeren Buus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lars Oestergaard Pedersen, Soeren Buus filed Critical Lars Oestergaard Pedersen
Priority to BR9914491-3A priority Critical patent/BR9914491A/en
Priority to CA002343313A priority patent/CA2343313A1/en
Priority to AU55047/99A priority patent/AU771239C/en
Priority to MXPA01002674A priority patent/MXPA01002674A/en
Priority to JP2000570203A priority patent/JP2002524104A/en
Priority to NZ511031A priority patent/NZ511031A/en
Priority to EP99941420A priority patent/EP1114063A2/en
Priority to KR1020017003306A priority patent/KR20010075108A/en
Publication of WO2000015665A2 publication Critical patent/WO2000015665A2/en
Publication of WO2000015665A3 publication Critical patent/WO2000015665A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell, where the gene is expressible, isolating the protein from the cell without reducing it and subjecting the isolated protein to a folding treatment. Preferably, the immunoglobulin superfamily protein is selected from the group consisting of Class I and Class II histocompatibility molecules and beta 2 microglobulin (beta2m). Other embodiments of the invention is a stable peptide free MHC protein obtainable by a process of the invention and a kit comprising a MHC class I heavy chain and a abeta2m allowing the recipient to produce and measure or detect a functional MHC class I protein to which a peptide, which is capabe of binding to said MHC class I protein, can be added leading to the generation of a functional MHC class I protein.
PCT/DK1999/000484 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein WO2000015665A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BR9914491-3A BR9914491A (en) 1998-09-14 1999-09-14 Process for producing a functional immunoglobulin superfamily protein
CA002343313A CA2343313A1 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
AU55047/99A AU771239C (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
MXPA01002674A MXPA01002674A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein.
JP2000570203A JP2002524104A (en) 1998-09-14 1999-09-14 Methods for producing functionalized immunoglobulin superfamily proteins
NZ511031A NZ511031A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
EP99941420A EP1114063A2 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein
KR1020017003306A KR20010075108A (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199801155 1998-09-14
DKPA199801155 1998-09-14

Publications (2)

Publication Number Publication Date
WO2000015665A2 WO2000015665A2 (en) 2000-03-23
WO2000015665A3 true WO2000015665A3 (en) 2000-08-17

Family

ID=8101632

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1999/000484 WO2000015665A2 (en) 1998-09-14 1999-09-14 A method of producing a functional immunoglobulin superfamily protein

Country Status (10)

Country Link
EP (1) EP1114063A2 (en)
JP (1) JP2002524104A (en)
KR (1) KR20010075108A (en)
CN (1) CN1326466A (en)
AU (1) AU771239C (en)
BR (1) BR9914491A (en)
CA (1) CA2343313A1 (en)
MX (1) MXPA01002674A (en)
NZ (1) NZ511031A (en)
WO (1) WO2000015665A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8969042B2 (en) 2004-08-19 2015-03-03 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
US9138461B2 (en) 2007-05-01 2015-09-22 Biogen Ma Inc. Compositions and methods for increasing vascularization
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2336167B1 (en) 2001-03-14 2019-05-29 Dako Denmark A/S MHC molecule constructs and their uses for diagnosis and therapy
AU2003223936A1 (en) * 2002-05-17 2003-12-02 Kobenhavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration
US20040072262A1 (en) 2002-10-11 2004-04-15 Montero-Julian Felix A. Methods and systems for detecting MHC class I binding peptides
WO2004072112A2 (en) * 2003-02-11 2004-08-26 Københavns Universitet A method for formulating mhc-like molecules for long time storage
WO2005043121A2 (en) * 2003-10-31 2005-05-12 Vitatex, Inc. Blood test prototypes and methods for the detection of circulating tumor and endothelial cells
EP2226332A1 (en) 2004-06-17 2010-09-08 Beckman Coulter, Inc. Mycobacterium tuberculosis epitopes and methods of use thereof
TWI501774B (en) 2006-02-27 2015-10-01 Biogen Idec Inc Treatments for neurological disorders
CL2007002225A1 (en) * 2006-08-03 2008-04-18 Astrazeneca Ab SPECIFIC UNION AGENT FOR A RECEIVER OF THE GROWTH FACTOR DERIVED FROM PLATES (PDGFR-ALFA); NUCLEIC ACID MOLECULA THAT CODIFIES IT; VECTOR AND CELL GUESTS THAT UNDERSTAND IT; CONJUGADO UNDERSTANDING THE AGENT; AND USE OF THE AGENT OF A
EP2155782A2 (en) 2007-03-26 2010-02-24 Dako Denmark A/S Mhc peptide complexes and uses thereof in infectious diseases
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
CN119241636A (en) 2007-07-09 2025-01-03 健泰科生物技术公司 Preventing disulfide bond reduction during recombinant production of peptides
WO2009039854A2 (en) 2007-09-27 2009-04-02 Dako Denmark A/S Mhc multimers in tuberculosis diagnostics, vaccine and therapeutics
GB2453589A (en) 2007-10-12 2009-04-15 King S College London Protease inhibition
US10968269B1 (en) 2008-02-28 2021-04-06 Agilent Technologies, Inc. MHC multimers in borrelia diagnostics and disease
US10722562B2 (en) 2008-07-23 2020-07-28 Immudex Aps Combinatorial analysis and repair
US10369204B2 (en) 2008-10-02 2019-08-06 Dako Denmark A/S Molecular vaccines for infectious disease
US11992518B2 (en) 2008-10-02 2024-05-28 Agilent Technologies, Inc. Molecular vaccines for infectious disease
CN103364558A (en) * 2013-07-17 2013-10-23 江阴泽成生物技术有限公司 Human tumor marker carcinoembryonic antigen (CEA) magnetic particle chemiluminiscence immunoassay kit and detection method
CN109402036A (en) * 2018-11-07 2019-03-01 河北科技师范学院 The application of ox source Salmonella enteritidis trxB gene delection
WO2020127222A2 (en) 2018-12-17 2020-06-25 Immudex Aps Panel comprising borrelia mhc multimers

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993022332A2 (en) * 1992-04-24 1993-11-11 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
WO1994006813A1 (en) * 1992-09-15 1994-03-31 The Rockefeller University Major histocompatibility complex molecules and modifications thereof
WO1995011702A1 (en) * 1993-10-25 1995-05-04 Anergen, Inc. Prokaryotic expression of mhc proteins
US5529921A (en) * 1992-02-19 1996-06-25 Scripps Research Institute In vitro activation of cytotoxic t-cells using insect cells expressing human class I MHC and β2-microglobulin
WO1997044667A2 (en) * 1996-05-21 1997-11-27 Institut Pasteur Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5529921A (en) * 1992-02-19 1996-06-25 Scripps Research Institute In vitro activation of cytotoxic t-cells using insect cells expressing human class I MHC and β2-microglobulin
WO1993022332A2 (en) * 1992-04-24 1993-11-11 Board Of Regents, The University Of Texas System Recombinant production of immunoglobulin-like domains in prokaryotic cells
WO1994006813A1 (en) * 1992-09-15 1994-03-31 The Rockefeller University Major histocompatibility complex molecules and modifications thereof
WO1995011702A1 (en) * 1993-10-25 1995-05-04 Anergen, Inc. Prokaryotic expression of mhc proteins
WO1997044667A2 (en) * 1996-05-21 1997-11-27 Institut Pasteur Method for using peptide complexes/major histocompatibility complex to obtain or purify antigen-specific t cells and to stimulate t cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; MOLLOY P E ET AL: "Production of soluble single-chain T-cell receptor fragments in Escherichia coli trxB mutants.", XP002131051, retrieved from STN Database accession no. 1998346783 *
GARBOCZI D N ET AL: "HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (1992 APR 15) 89 (8) 3429-33., XP002131059 *
MOLECULAR IMMUNOLOGY, (1998 FEB) 35 (2) 73-81. *
PARKER K C ET AL: "An HLA-A2/beta 2-microglobulin/peptide complex assembled from subunits expressed separately in Escherichia coli.", MOLECULAR IMMUNOLOGY, (1992 MAR) 29 (3) 371-8., XP000876800 *
PLAKSIN D ET AL: "A T cell receptor V alpha domain expressed in bacteria: does it dimerize in solution?.", JOURNAL OF EXPERIMENTAL MEDICINE, (1996 OCT 1) 184 (4) 1251-8., XP000876791 *
STEVENS JAMES ET AL: "Efficient generation of major histocompatibility complex class I-peptide complexes using synthetic peptide libraries.", JOURNAL OF BIOLOGICAL CHEMISTRY JAN. 30, 1998, vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2874 - 2884, XP002136475, ISSN: 0021-9258 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8969042B2 (en) 2004-08-19 2015-03-03 Biogen Idec Ma Inc. Refolding transforming growth factor beta family proteins
US9138461B2 (en) 2007-05-01 2015-09-22 Biogen Ma Inc. Compositions and methods for increasing vascularization
US9404916B2 (en) 2008-09-20 2016-08-02 University College Cardiff Consultants Limited Use of a protein kinase inhibitor to detect immune cells, such as T cells

Also Published As

Publication number Publication date
EP1114063A2 (en) 2001-07-11
AU771239B2 (en) 2004-03-18
CA2343313A1 (en) 2000-03-23
AU771239C (en) 2004-10-14
WO2000015665A2 (en) 2000-03-23
KR20010075108A (en) 2001-08-09
AU5504799A (en) 2000-04-03
CN1326466A (en) 2001-12-12
BR9914491A (en) 2002-01-15
MXPA01002674A (en) 2002-06-04
NZ511031A (en) 2003-11-28
JP2002524104A (en) 2002-08-06

Similar Documents

Publication Publication Date Title
WO2000015665A3 (en) A method of producing a functional immunoglobulin superfamily protein
WO2003059935A3 (en) Methods for purifying protein
WO1997026270A3 (en) Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
AU9125198A (en) Compositions, kits, and methods for effecting adenine nucleotide modulation of dna mismatch recognition proteins
WO2002072605A3 (en) Expression technology for proteins containing a hybrid isotype antibody moiety
WO1996018731A3 (en) Isolation of nucleic acid
EP0930365A3 (en) Binding domains in serrate protein
WO1999042077A3 (en) Compositions and methods for regulating lymphocyte activation
WO1999057280A3 (en) Neisseria meningitidis antigens and compositions
EP2330197A3 (en) Antagonistic selective binding agents of osteoprotegerin binding protein
WO2003020932A1 (en) Novel secretory proteins and dna thereof
AU2002235994A1 (en) Method for producing proteins by fermentation of microorganisms from the thermus family, the protein mixture thus obtained and cosmetic compositions containing same
DE69604240D1 (en) Polyarylene sulfide, process for its preparation and this comprehensive mass
ATE227579T1 (en) METHOD FOR PRODUCING MACROPHAGES AND EQUIPMENT FOR CARRYING OUT SUCH METHOD
CA2079454A1 (en) Steroid 5.alpha.-reductases
GR3019849T3 (en) Acute pancreatitis associated protein and means for the diagnosis of acute pancreatitis
WO1997043409A3 (en) Method of providing novel dna sequences
WO1995029236A3 (en) Lymphocyte activation antigens and antibodies thereto
WO1996027665A3 (en) Neuron-restrictive silencer factor proteins
EP1980573A3 (en) Isolated dendritic cell membrane protein genes
WO1996007739A3 (en) Interleukin-1 type 3 receptors
WO1998032858A3 (en) Mammalian chemokines; receptors; reagents; uses
WO1999047558A3 (en) T cell costimulatory proteins, sequences and uses thereof
AU3711597A (en) A novel galanin receptor
WO2000018909A3 (en) NUCLEIC ACIDS AND PROTEINS FROM $i(CENARCHAEUM SYMBIOSUM)

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99813211.X

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ CZ DE DE DK DK EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ CZ DE DE DK DK EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

ENP Entry into the national phase

Ref document number: 2343313

Country of ref document: CA

Ref document number: 2343313

Country of ref document: CA

Kind code of ref document: A

Ref document number: 2000 570203

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 1020017003306

Country of ref document: KR

Ref document number: PA/a/2001/002674

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 55047/99

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 511031

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 1999941420

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09787032

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999941420

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1020017003306

Country of ref document: KR

WWG Wipo information: grant in national office

Ref document number: 55047/99

Country of ref document: AU

WWW Wipo information: withdrawn in national office

Ref document number: 1999941420

Country of ref document: EP

WWR Wipo information: refused in national office

Ref document number: 1020017003306

Country of ref document: KR

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载