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WO2000009720A3 - Cytochrome b5 from petunia - Google Patents

Cytochrome b5 from petunia Download PDF

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Publication number
WO2000009720A3
WO2000009720A3 PCT/GB1999/002676 GB9902676W WO0009720A3 WO 2000009720 A3 WO2000009720 A3 WO 2000009720A3 GB 9902676 W GB9902676 W GB 9902676W WO 0009720 A3 WO0009720 A3 WO 0009720A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
transgenic plants
cytochrome
stems
nucleotides
Prior art date
Application number
PCT/GB1999/002676
Other languages
French (fr)
Other versions
WO2000009720A2 (en
Inventor
Ronald Koes
Vetten Nick De
Jos Mol
Original Assignee
Int Flower Dev Pty Ltd
Vrije Universiteit
Jones Elizabeth
Ronald Koes
Vetten Nick De
Jos Mol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Flower Dev Pty Ltd, Vrije Universiteit, Jones Elizabeth, Ronald Koes, Vetten Nick De, Jos Mol filed Critical Int Flower Dev Pty Ltd
Priority to AU53815/99A priority Critical patent/AU5381599A/en
Publication of WO2000009720A2 publication Critical patent/WO2000009720A2/en
Publication of WO2000009720A3 publication Critical patent/WO2000009720A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates generally to a plant regulatory gene and derivatives and homologues thereof. More particularly, the present invention provides a nucleic acid molecule comprising a sequence of nucleotides or complementary sequence of nucleotides, the expression of which, modulates or otherwise facilitates activity of a cytochrome P450 protein in plant cells and tissues including petals, flowers, stems, leaves and seeds. Even more particularly, the nucleic acid molecule of the present invention encodes a cytochrome b5 or a mutant, part, fragment, portion, functional and/or structural equivalent or homologue thereof or agonist or antagonist thereof involved in modulating or otherwise facilitating activity of a dihydrokaempferol (DHK) hydroxylating enzyme such as but not limited to flavonoid 3', 5'-hydroxylase. The present invention further provides transgenic plants or parts thereof or cells of transgenic plants as well as cut or severed flowers or stems from transgenic plants.
PCT/GB1999/002676 1998-08-14 1999-08-13 Cytochrome b5 from petunia WO2000009720A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU53815/99A AU5381599A (en) 1998-08-14 1999-08-13 Cytochrome b5 from petunia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPP5268 1998-08-14
AUPP5268A AUPP526898A0 (en) 1998-08-14 1998-08-14 A plant regulatory gene

Publications (2)

Publication Number Publication Date
WO2000009720A2 WO2000009720A2 (en) 2000-02-24
WO2000009720A3 true WO2000009720A3 (en) 2000-05-18

Family

ID=3809500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/002676 WO2000009720A2 (en) 1998-08-14 1999-08-13 Cytochrome b5 from petunia

Country Status (2)

Country Link
AU (1) AUPP526898A0 (en)
WO (1) WO2000009720A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003254410B2 (en) * 2002-08-30 2011-04-21 Suntory Holdings Limited Flavonoid 3',5'hydroxylase gene sequences and uses therefor
WO2004020637A1 (en) * 2002-08-30 2004-03-11 International Flower Developments Pty. Ltd. Flavonoid 3',5'hydroxylase gene sequences and uses therefor
AU2003901797A0 (en) 2003-04-14 2003-05-01 Agresearch Limited Manipulation of condensed tannin biosynthesis
US20110023162A1 (en) * 2007-11-15 2011-01-27 International Flower Developments Pty. Ltd. Genetically modified chrysanthemums

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993001290A1 (en) * 1991-07-11 1993-01-21 International Flower Developments Pty. Ltd. Genetic sequences encoding flavonoid pathway enzymes and uses therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993001290A1 (en) * 1991-07-11 1993-01-21 International Flower Developments Pty. Ltd. Genetic sequences encoding flavonoid pathway enzymes and uses therefor

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DE VETTEN N ET AL: "A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 96, no. 2, January 1999 (1999-01-01), pages 778 - 783, XP002129202 *
KEARNS E V ET AL.: "Primary structure of cytochrome b5 from cauliflower (Brassica oleracea L.) deduced from peptide and cDNA sequences", PLANT PHYSIOOGY, vol. 99, 1992, pages 1254 - 1257, XP002129201 *
MARTSINKOVSKAYA A I ET AL: "Temporal and spatial gene expression of cytochrome B5 during flower and fruit development in olives", PLANT MOLECULAR BIOLOGY, vol. 40, no. 1, May 1999 (1999-05-01), pages 79 - 90, XP002129203 *
SAYANOVA O ET AL: "Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of hig levels of Delta6-desaturated fatty acids in transgenic tobacco", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 94, 15 April 1997 (1997-04-15), pages 4211 - 4216, XP002106758, ISSN: 0027-8424 *
SMITH M A ET AL.: "Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro protein targeting", PLANT MOLECULAR BIOLOGY, vol. 25, no. 3, June 1994 (1994-06-01), pages 527 - 537, XP002129200 *

Also Published As

Publication number Publication date
WO2000009720A2 (en) 2000-02-24
AUPP526898A0 (en) 1998-09-10

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