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WO2000008190A3 - Sterol methyltransferase gene - Google Patents

Sterol methyltransferase gene Download PDF

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Publication number
WO2000008190A3
WO2000008190A3 PCT/US1999/017456 US9917456W WO0008190A3 WO 2000008190 A3 WO2000008190 A3 WO 2000008190A3 US 9917456 W US9917456 W US 9917456W WO 0008190 A3 WO0008190 A3 WO 0008190A3
Authority
WO
WIPO (PCT)
Prior art keywords
sterol
plant
methyltransferase
disclosed
ergosterol
Prior art date
Application number
PCT/US1999/017456
Other languages
French (fr)
Other versions
WO2000008190A2 (en
Inventor
Robert J Grebenok
Original Assignee
Univ Arizona
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arizona filed Critical Univ Arizona
Priority to AU52503/99A priority Critical patent/AU5250399A/en
Publication of WO2000008190A2 publication Critical patent/WO2000008190A2/en
Publication of WO2000008190A3 publication Critical patent/WO2000008190A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed is an isolated DNA sequence that encodes a Zea mays C-24 sterol methyltransferase. A yeast erg6 transformant containing the C-24 sterol methyltransferase gene produces ergosterol, whereas untransformed isolates of yeast erg6 do not produce ergosterol. Also disclosed is a method of altering sterol metabolism in a plant by transforming the plant with a heterologous construct comprising a Z. mays C-24 methyltransferase coding sequence operably connected to a plant promoter. Plants having altered sterol metabolism are expected to exhibit unique phenotypes, including reduced ability to support feeding pests that depend on plant sterols for completion of their life cycles.
PCT/US1999/017456 1998-08-03 1999-08-02 Sterol methyltransferase gene WO2000008190A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU52503/99A AU5250399A (en) 1998-08-03 1999-08-02 Sterol methyltransferase gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12833998A 1998-08-03 1998-08-03
US09/128,339 1998-08-03

Publications (2)

Publication Number Publication Date
WO2000008190A2 WO2000008190A2 (en) 2000-02-17
WO2000008190A3 true WO2000008190A3 (en) 2000-06-08

Family

ID=22434854

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/017456 WO2000008190A2 (en) 1998-08-03 1999-08-02 Sterol methyltransferase gene

Country Status (2)

Country Link
AU (1) AU5250399A (en)
WO (1) WO2000008190A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000061771A2 (en) * 1999-04-12 2000-10-19 Monsanto Technology Llc Transgenic plants containing altered levels of sterol compounds and tocopherols
CA2406076A1 (en) * 2000-04-14 2001-10-25 Unilever Plc Process for modifying plants
US6977269B1 (en) 2001-05-03 2005-12-20 Pioneer Hi-Bred International, Inc Method of improving animal tissue quality
EP1871883A1 (en) 2005-03-02 2008-01-02 Metanomics GmbH Process for the production of fine chemicals
US20090138988A1 (en) * 2007-07-21 2009-05-28 Keri Wang Modification of plant disease resistance
CN116083454B (en) * 2022-07-15 2024-11-29 自然资源部第三海洋研究所 Deep sea tubular worm SMT gene and expression method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0480730A2 (en) * 1990-10-12 1992-04-15 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
WO1998045457A1 (en) * 1996-12-26 1998-10-15 Monsanto Company Transgenic plants with modified sterol biosynthetic pathways

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0480730A2 (en) * 1990-10-12 1992-04-15 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
WO1998045457A1 (en) * 1996-12-26 1998-10-15 Monsanto Company Transgenic plants with modified sterol biosynthetic pathways

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BOUVIER-NAVE P. ET AL.: "Identification of cDNAs encoding sterol methyl-transferases involved in a second methylation step of plant sterol biosynthesis.", EUR J BIOCHEM 1997 JUN 1;246(2):518-29, XP000879356 *
GREBENOK, R.J. ETAL.: "characterization of Zea mays endosperm C-24 sterol methyltransferase: one of two types of sterol methyltransferase in higher plants", EMBL SEQUENCE DATA LIBRARY, 21 March 1997 (1997-03-21), heidelberg, germany *
GREBENOK, R.J., ET AL.: "characterization of Zea mays endosperm C-24 methyltransferase: one of two types of sterol methyltransferase in higher plants", PLANT MOLECULAR BIOLOGY, vol. 34, 1997, pages 891 - 896, XP002130677 *
GUO D -A ET AL: "Phytosterol Biosynthesis: Isotope Effects Associated with Biomethylation Formation to 24-Alkene Sterol Isomers", TETRAHEDRON LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 37, no. 38, 16 September 1996 (1996-09-16), pages 6823 - 6826, XP004030759, ISSN: 0040-4039 *
HUSSELSTEIN, T., ET AL.: "transformation of Saccharomyces cerevisae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols", FEBS LETTERS, vol. 381, 1996, pages 87 - 92, XP002130679 *
NES, W.D., ET AL.: "structural requirements for transformation od substrates by the (S)-Adenosyl-L-methionine:delta 24(25) -sterol methyl transferase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 23, 1991, pages 15202 - 15212, XP002130681 *
SCHALLER H. ET AL.: "Overexpression of an Arabidopsis cDNA encoding a sterol-C24(1)-methyltransferase in tobacco modifies the ratio of 24-methyl cholesterol to sitosterol and is associated with growth reduction.", PLANT PHYSIOL 1998 OCT;118(2):461-9, XP002130682 *
SHI, J., ET AL.: "identification and characterization of an S-adenosyl-L-methionine: delta24-sterol-c-methyltransferase cDNA from soybean", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 16, April 1996 (1996-04-01), pages 9384 - 9389, XP002130680 *
TONG, Y. AND NES, W.D.: "untitled", EMBL SEQUENCE DATA LIBRARY, 11 February 1998 (1998-02-11), heidelberg, germany, XP002131340 *

Also Published As

Publication number Publication date
AU5250399A (en) 2000-02-28
WO2000008190A2 (en) 2000-02-17

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