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WO2000008164A3 - Thermostable in vitro complex with polymerase activity - Google Patents

Thermostable in vitro complex with polymerase activity Download PDF

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Publication number
WO2000008164A3
WO2000008164A3 PCT/DE1999/002480 DE9902480W WO0008164A3 WO 2000008164 A3 WO2000008164 A3 WO 2000008164A3 DE 9902480 W DE9902480 W DE 9902480W WO 0008164 A3 WO0008164 A3 WO 0008164A3
Authority
WO
WIPO (PCT)
Prior art keywords
thermostable
polymerase activity
vitro complex
vitro
complex
Prior art date
Application number
PCT/DE1999/002480
Other languages
German (de)
French (fr)
Other versions
WO2000008164A2 (en
Inventor
Christian Kilger
Ingo Kober
Hartmut Voss
Gerd Moeckel
Original Assignee
Lion Bioscience Ag
Christian Kilger
Ingo Kober
Hartmut Voss
Gerd Moeckel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19840771A external-priority patent/DE19840771A1/en
Application filed by Lion Bioscience Ag, Christian Kilger, Ingo Kober, Hartmut Voss, Gerd Moeckel filed Critical Lion Bioscience Ag
Priority to EP99942776A priority Critical patent/EP1100923A2/en
Priority to DE19981494T priority patent/DE19981494D2/en
Priority to JP2000563788A priority patent/JP2002522042A/en
Priority to CA002338185A priority patent/CA2338185A1/en
Priority to AU56171/99A priority patent/AU5617199A/en
Publication of WO2000008164A2 publication Critical patent/WO2000008164A2/en
Publication of WO2000008164A3 publication Critical patent/WO2000008164A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The inventive thermostable in vitro complex for template-dependent elongation of nucleic acids comprises a thermostable staple protein and a thermostable elongation protein.
PCT/DE1999/002480 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity WO2000008164A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP99942776A EP1100923A2 (en) 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity
DE19981494T DE19981494D2 (en) 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity
JP2000563788A JP2002522042A (en) 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity
CA002338185A CA2338185A1 (en) 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity
AU56171/99A AU5617199A (en) 1998-08-06 1999-08-06 Thermostable (in vitro) complex with polymerase activity

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19835653 1998-08-06
DE19835653.6 1998-08-06
DE19840771A DE19840771A1 (en) 1998-08-06 1998-09-07 A thermostable in vitro polymerase complex for template-dependent elongation of nucleic acids in amplification or reverse transcription methods
DE19840771.8 1998-09-07
EP99111795.3 1999-06-18
EP99111795 1999-06-18

Publications (2)

Publication Number Publication Date
WO2000008164A2 WO2000008164A2 (en) 2000-02-17
WO2000008164A3 true WO2000008164A3 (en) 2000-05-11

Family

ID=56289931

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/002480 WO2000008164A2 (en) 1998-08-06 1999-08-06 Thermostable in vitro complex with polymerase activity

Country Status (5)

Country Link
EP (1) EP1100923A2 (en)
JP (1) JP2002522042A (en)
AU (1) AU5617199A (en)
CA (1) CA2338185A1 (en)
WO (1) WO2000008164A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2792651B1 (en) * 1999-04-21 2005-03-18 Centre Nat Rech Scient GENOMIC SEQUENCE AND POLYPEPTIDES OF PYROCOCCUS ABYSSI, THEIR FRAGMENTS AND USES THEREOF
DE19937230A1 (en) * 1999-08-06 2001-02-08 Lion Bioscience Ag Chimeric proteins
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
EP1414852A2 (en) * 2001-08-10 2004-05-06 Genset S.A. Human secreted proteins, their encoding pulynucleotides, and uses thereof
JP6968536B2 (en) * 2014-11-28 2021-11-17 東洋紡株式会社 Nucleic acid amplification reagent
JP6699560B2 (en) * 2014-11-28 2020-05-27 東洋紡株式会社 PCNA monomer
EP3381947A4 (en) * 2015-11-27 2019-04-10 Kyushu University National University Corporation MUTANT DNA POLYMERASE

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993015115A1 (en) * 1992-01-24 1993-08-05 Cornell Research Foundation, Inc. E. coli dna polymerase iii holoenzyme and subunits
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999053074A1 (en) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993015115A1 (en) * 1992-01-24 1993-08-05 Cornell Research Foundation, Inc. E. coli dna polymerase iii holoenzyme and subunits
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999053074A1 (en) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MCHENRY C S ET AL: "A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium", JOURNAL OF MOLECULAR BIOLOGY,GB,LONDON, XP002075007, ISSN: 0022-2836 *
YURIEVA O ET AL: "Thermus thermophilis dnaX homolog encoding gamma- and tau-like proteins of the chromosomal replicase", JOURNAL OF BIOLOGICAL CHEMISTRY. (MICROFILMS),US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, XP002075006 *
Z. KELMAN AND M. O'DONNEL: "Structural and functional similarities of procaryotic and eucaryotic DNA polymerase sliding clamps", NUCLEIC ACIDS RESEARCH, vol. 23, no. 18, 1995, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 3613 - 3620, XP002128922 *

Also Published As

Publication number Publication date
CA2338185A1 (en) 2000-02-17
EP1100923A2 (en) 2001-05-23
JP2002522042A (en) 2002-07-23
AU5617199A (en) 2000-02-28
WO2000008164A2 (en) 2000-02-17

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