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WO2000001824A3 - Recombinaison genetique de la resistance aux maladies au moyen de la classe de proteines drr206 - Google Patents

Recombinaison genetique de la resistance aux maladies au moyen de la classe de proteines drr206 Download PDF

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Publication number
WO2000001824A3
WO2000001824A3 PCT/CA1999/000608 CA9900608W WO0001824A3 WO 2000001824 A3 WO2000001824 A3 WO 2000001824A3 CA 9900608 W CA9900608 W CA 9900608W WO 0001824 A3 WO0001824 A3 WO 0001824A3
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WO
WIPO (PCT)
Prior art keywords
drr206
defensin
maculans
resistance
chitinase
Prior art date
Application number
PCT/CA1999/000608
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English (en)
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WO2000001824A2 (fr
Inventor
Brian Fristensky
Yaping Wang
Original Assignee
Univ Manitoba
Brian Fristensky
Yaping Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA 2242116 external-priority patent/CA2242116A1/fr
Application filed by Univ Manitoba, Brian Fristensky, Yaping Wang filed Critical Univ Manitoba
Priority to AU44945/99A priority Critical patent/AU4494599A/en
Priority to CA002337095A priority patent/CA2337095A1/fr
Publication of WO2000001824A2 publication Critical patent/WO2000001824A2/fr
Publication of WO2000001824A3 publication Critical patent/WO2000001824A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Pour identifier des gènes efficaces contre le champignon de la jambe noire Leptosphaeria maculans, nous avons transformé un colza canola (Brassica napus) avec quatre gènes de pois (Pisum sativum) sous régulation constitutive du promoteur CaMV 35S, à savoir les gènes PR10.1, chitinase, DRR206 et défensine. Les lignées transgéniques contenant des insertions d'ADN-T à copie unique pour chaque gène ont été criblés en vue de rechercher une résistance présente à la fois sur les semis (cotylédonaire) et sur la plante adulte. Les lignées transgéniques présentant une expression d'ARNm de DRR206 ou de défensine de pois ont montré des taux de maladie réduit après inoculation de L. maculans ou de Sclerotinia sclerotiorum, comparés aux lignées transgéniques n'exprimant pas ces gènes. On n'a en revanche constaté que peu voire pas d'augmentation de la résistance dans les lignées transgéniques exprimant PR10 et la chitinase. La résistance à L.maculans a en outre fait preuve d'une co-ségrégation avec les transgènes DRR206 et défensine. Des extraits de plantes transgéniques DRR206 et défensine ont produit une inhibition de la prolifération fongique in vitro. Dans les plantes transgéniques DRR206 on a également constaté une réduction de la prolifération fongique sur les sites d'inoculation, ainsi que des signes d'une réaction d'hypersensibilité.
PCT/CA1999/000608 1998-07-03 1999-07-02 Recombinaison genetique de la resistance aux maladies au moyen de la classe de proteines drr206 WO2000001824A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU44945/99A AU4494599A (en) 1998-07-03 1999-07-02 Method for genetic engineering of disease resistance using the drr206 class of proteins
CA002337095A CA2337095A1 (fr) 1998-07-03 1999-07-02 Recombinaison genetique de la resistance aux maladies au moyen de la classe de proteines drr206

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA 2242116 CA2242116A1 (fr) 1998-07-03 1998-07-03 Methode de genie genetique liee a la resistance a la maladie utilisant la categorie de proteines dr206
CA2,242,116 1998-07-03
US9175198P 1998-07-06 1998-07-06
US60/091,751 1998-07-06

Publications (2)

Publication Number Publication Date
WO2000001824A2 WO2000001824A2 (fr) 2000-01-13
WO2000001824A3 true WO2000001824A3 (fr) 2000-02-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1999/000608 WO2000001824A2 (fr) 1998-07-03 1999-07-02 Recombinaison genetique de la resistance aux maladies au moyen de la classe de proteines drr206

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Country Link
AU (1) AU4494599A (fr)
WO (1) WO2000001824A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855865B2 (en) * 1999-05-07 2005-02-15 E.I. Du Pont De Nemours And Company Nucleic acids encoding plant defensins and methods of use thereof
WO2009043182A1 (fr) * 2007-10-05 2009-04-09 University Of Manitoba Marqueurs de région amplifiée de séquence connue (scar) pour la résistance de plantes à des pathogènes fongiques

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993005153A1 (fr) * 1991-08-29 1993-03-18 Zeneca Limited Proteines biocides
WO1993019188A1 (fr) * 1992-03-20 1993-09-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Gene chimerique sensible aux champignons
US5312912A (en) * 1989-06-13 1994-05-17 Hadwiger Lee A Procedures and regulatory DNA sequences for genetically engineering disease resistance and other inducible traits in plants
WO1997041237A1 (fr) * 1996-04-29 1997-11-06 COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION (as a participant in the COOPERATIVE RESEARCH CENTRE FOR TROPICAL PLANT PATHOLOGY) Plantes transgeniques resistantes aux champignons
WO1998026083A1 (fr) * 1996-12-13 1998-06-18 Monsanto Company Polypeptide antifongique et procedes de lutte contre les champignons pathogenes des plantes

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5312912A (en) * 1989-06-13 1994-05-17 Hadwiger Lee A Procedures and regulatory DNA sequences for genetically engineering disease resistance and other inducible traits in plants
WO1993005153A1 (fr) * 1991-08-29 1993-03-18 Zeneca Limited Proteines biocides
WO1993019188A1 (fr) * 1992-03-20 1993-09-30 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Gene chimerique sensible aux champignons
WO1997041237A1 (fr) * 1996-04-29 1997-11-06 COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION (as a participant in the COOPERATIVE RESEARCH CENTRE FOR TROPICAL PLANT PATHOLOGY) Plantes transgeniques resistantes aux champignons
WO1998026083A1 (fr) * 1996-12-13 1998-06-18 Monsanto Company Polypeptide antifongique et procedes de lutte contre les champignons pathogenes des plantes

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
BROWN, S.M., ET AL.: "Sequence conservation of a family of disease response genes among pea species and their expression in transgenic canola", J. CELL. BIOCHEM. SUPPL., vol. 18a, 1994, pages 86, XP000857499 *
CANADIAN JOURNAL OF PLANT PATHOLOGY, (MARCH, 1988) VOL. 20, NO.1, PP131. MEETING INFO: ANNUAL MEETING OF THE CANADIAN PHYTOPATHOLOGICAL SOCIETY WINNIPEG, MANITOBA, CANADA JULY 1997 *
CHIANG CH C ET AL: "THE FUSARIUM SOLANI-INDUCED EXPRESSION OF A PEA GENE FAMILY ENCODING HIGH CYSTEINE CONTENT PROTEINS", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 4, no. 4, 1991, pages 324-331, XP002063334, ISSN: 0894-0282 *
CULLEY, D.E., ET AL.: "Pisum sativum disease resistance response protein 206-d (DRR206-d) gene, complete cds", EMBL ACCESSION NO: U11716, 15 July 1994 (1994-07-15), XP002125566 *
CULLEY, DAVID E. ET AL: "Molecular characterization of disease-resistance response gene DRR206 -d from Pisum sativum (L.)", PLANT PHYSIOL. (1995), 107(1), 301-2, XP002125565 *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; WANG, Y., ET AL.: "Expression of native and constitutively-expressed pea defense genes in transgenic plants", XP002125573 *
DATABASE SCISEARCH "FIELD TOLERANCE TO FUNGAL PATHOGENS OF BRASSICA-NAPUS CONSTITUTIVELY EXPRESSING A CHIMERIC CHITINASE GENE", XP002107279 *
DATABASE SCISEARCH WANG, Y.P., ET AL.: "Constitutive expression of pea defense gene DRR206 confers resistance to blackleg (Leptosphaeria maculans) disease in transgenic canola (Brassica napus)", XP002125574 *
FRISTENSKY, B., ET AL.: "cDNA sequences for pea disease resistance response genes", PLANT MOLECULAR BIOLOGY, vol. 11, 1988, pages 713 - 715, XP002125569 *
MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 12, no. 5, May 1999 (1999-05-01), pages 410 - 418 *
MOLONEY, M.M., ET AL.: "High efficiency transformation of Brassica napus using Agrobacterium vectors", PLANT CELL REPORTS, vol. 8, 1989, pages 238 - 242, XP002125570 *
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TERRAS F R G ET AL: "SMALL CYSTEINE-RICH ANTIFUNGAL PROTEINS FROM RADISH: THEIR ROLE IN HOST DEFENSE", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 7, no. 5, May 1995 (1995-05-01), pages 573-588, XP002029836, ISSN: 1040-4651 *
WANG, Y., ET AL.: "Pisum sativun pathogenesis-related protein (DRR206-c) gene, complete cds", EMBL ACCESSION NO: AF115574, 20 April 1999 (1999-04-20), XP002125567 *

Also Published As

Publication number Publication date
AU4494599A (en) 2000-01-24
WO2000001824A2 (fr) 2000-01-13

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