+

WO2000000638A3 - Tagging of rna amplicons generated by transcription-based amplification - Google Patents

Tagging of rna amplicons generated by transcription-based amplification Download PDF

Info

Publication number
WO2000000638A3
WO2000000638A3 PCT/EP1999/004290 EP9904290W WO0000638A3 WO 2000000638 A3 WO2000000638 A3 WO 2000000638A3 EP 9904290 W EP9904290 W EP 9904290W WO 0000638 A3 WO0000638 A3 WO 0000638A3
Authority
WO
WIPO (PCT)
Prior art keywords
transcription
based amplification
amplicons
tagging
rna
Prior art date
Application number
PCT/EP1999/004290
Other languages
French (fr)
Other versions
WO2000000638A2 (en
Inventor
Peter Theodorus Gera Sillekens
Deursen Petrus Bernardus H Van
Wessel Kok
Original Assignee
Akzo Nobel Nv
Peter Theodorus Gera Sillekens
Deursen Petrus Bernardus Hugo
Wessel Kok
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel Nv, Peter Theodorus Gera Sillekens, Deursen Petrus Bernardus Hugo, Wessel Kok filed Critical Akzo Nobel Nv
Priority to AU45156/99A priority Critical patent/AU4515699A/en
Publication of WO2000000638A2 publication Critical patent/WO2000000638A2/en
Publication of WO2000000638A3 publication Critical patent/WO2000000638A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention concerns a method for tagging the reaction products of transcription-based amplification procedures, and a kit for detecting such tagged nucleic acid amplified in a transcription-based amplification assay. The method is characterised in that RNA amplicons are generated by transcription-based amplification by introducing transcribable non-target related sequence elements in one or both primers used for transcription-based amplification which are transcribed into the RNA amplicons. In particular, the extra sequence elements thus introduced into the amplicons, can be used for the detection of the amplicons, for specific selection of the amplicons, to artificially extend the length of amplicons or to tag polypeptides translated from RNA amplicons generated by transcription-based amplification.
PCT/EP1999/004290 1998-06-26 1999-06-21 Tagging of rna amplicons generated by transcription-based amplification WO2000000638A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU45156/99A AU4515699A (en) 1998-06-26 1999-06-21 Tagging of rna amplicons generated by transcription-based amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98202142 1998-06-26
EP98202142.0 1998-06-26

Publications (2)

Publication Number Publication Date
WO2000000638A2 WO2000000638A2 (en) 2000-01-06
WO2000000638A3 true WO2000000638A3 (en) 2000-04-20

Family

ID=8233852

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004290 WO2000000638A2 (en) 1998-06-26 1999-06-21 Tagging of rna amplicons generated by transcription-based amplification

Country Status (2)

Country Link
AU (1) AU4515699A (en)
WO (1) WO2000000638A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2368809B (en) * 2000-09-15 2004-09-29 Norchip As Microfabricated reaction chamber system
WO2002029118A1 (en) * 2000-10-05 2002-04-11 Hong Kong Dna Chips Limited A kit for detecting non-pathogenic or pathogenic influenza a subtype h5 virus
DE60138286D1 (en) * 2001-10-30 2009-05-20 Hong Kong Dna Chips Ltd Method and kit for detection of foot-and-mouth disease virus
GB0200258D0 (en) * 2002-01-07 2002-02-20 Norchip As Detection of human papillomavirus
US7553623B2 (en) 2002-01-07 2009-06-30 Norchip A/S Method for detecting human papillomavirus mRNA
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
DE602005025424D1 (en) 2004-01-23 2011-01-27 Bio Merieux Inc Novel nucleotide mixture for improved nucleic acid amplification performance
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2121956B1 (en) 2006-12-21 2016-08-17 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
AU2014286889B2 (en) 2013-07-03 2019-05-23 Qvella Corporation Methods of targeted antibiotic susceptibility testing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication
WO1995002067A1 (en) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Improved method for the quantification of nucleic acid
WO1996002668A1 (en) * 1994-07-15 1996-02-01 Azco Nobel N.V. Use of rna polymerase to improve nucleic acid amplification process

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication
WO1995002067A1 (en) * 1993-07-09 1995-01-19 Akzo Nobel N.V. Improved method for the quantification of nucleic acid
WO1996002668A1 (en) * 1994-07-15 1996-02-01 Azco Nobel N.V. Use of rna polymerase to improve nucleic acid amplification process

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GEMEN VAN B ET AL: "A ONE-TUBE QUANTITATIVE HIV-1 RNA NASBA NUCLEIC ACID AMPLIFICATION ASSAY USING ELECTROCHEMILUMINESCENT (ECL) LABELLED PROBES", JOURNAL OF VIROLOGICAL METHODS, vol. 49, 1 January 1994 (1994-01-01), pages 157 - 167, XP000600186 *
KENTEN J H ET AL: "IMPROVED ELECTROCHEMILUMINESCENT LABEL FOR DNA PROBE ASSAYS: RAPID QUANTITATIVE ASSAYS OF HIV-1 POLYMERASE CHAIN REACTION PRODUCTS", CLINICAL CHEMISTRY, vol. 38, no. 6, 1992, pages 873 - 879, XP000606988 *
LIVACHE T ET AL: "DETECTION OF HIV1 DNA IN BIOLOGICAL SAMPLESS BY AN HOMOGENEOUS ASSAY: FLUORESCENCE MEASUREMENT OF DOUBLE-STRANDED RNA SYNTHESIZED FROM AMPLIFIED DNA", ANALYTICAL BIOCHEMISTRY,US,ACADEMIC PRESS, SAN DIEGO, CA, vol. 217, no. 2, 1 March 1994 (1994-03-01), pages 248 - 254, XP000425612, ISSN: 0003-2697 *
SECCHIERO P ET AL: "QUANTITATIVE PCR FOR HUMAN HERPESVIRUSES 6 AND 7", JOURNAL OF CLINICAL MICROBIOLOGY,US,WASHINGTON, DC, vol. 33, no. 8, 1 August 1995 (1995-08-01), pages 2124 - 2130, XP000564243, ISSN: 0095-1137 *

Also Published As

Publication number Publication date
AU4515699A (en) 2000-01-17
WO2000000638A2 (en) 2000-01-06

Similar Documents

Publication Publication Date Title
WO2000024931A3 (en) Detection of analytes
CA2218818A1 (en) Homogeneous methods for nucleic acid amplification and detection
EP0875584A3 (en) Nucleic acid assays
WO2001071042A8 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
WO2003020891A3 (en) Single nucleotide amplification and detection by polymerase
WO2001092579A3 (en) Methods for detecting target nucleic acids using coupled ligation and amplification
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
WO2005010209A3 (en) Method for the reverse transcription and/or amplification of nucleic acids
WO2000040755A3 (en) Method for accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
WO2001088089A8 (en) Single copy genomic hybridization probes and method of generating same
WO2002061133A3 (en) Nucleic acid detection using structured probes
WO2000000638A3 (en) Tagging of rna amplicons generated by transcription-based amplification
WO2002097132A3 (en) Nucleic acid detection method
WO1997007235A3 (en) All-in-one nucleic acid amplification assay
WO2004020654A3 (en) Solid phase based nucleic acid assays combining high affinity and high specificity
EP0803578A3 (en) Kit for amplification and detection of nucleic acid of cytomegalovirus (cmv) utilizing a nucleic acid sequence (ss2.7)
WO2002063049A3 (en) Methods for determining a nucleotide at a specific location within a nucleic acid molecule
CA2264967A1 (en) Method of dna sequencing
WO2002061143A3 (en) Comparative analysis of nucleic acids using population tagging
WO1996021042A3 (en) Primers for the pcr amplification of metastatic sequences
DE59914868D1 (en) DNA detection via a strand-annealing complex
WO1996015264A3 (en) Method for identifying nucleic acid sequences from different biological sources through amplification of the same
SG153624A1 (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid
WO2001029262A3 (en) Genotyping reagents, kits and methods of use thereof
WO1998026097A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(LEGIONELLA PNEUMOPHILA)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA ID JP KR US ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA ID JP KR US ZA

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA

CR1 Correction of entry in section i

Free format text: PAT. BUL. 01/2002 UNDER (25) AND (26) REPLACE "FRENCH" BY "ENGLISH"

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载