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WO2000000627A3 - Fatty acid hydroperoxide lyase nucleic acid sequences - Google Patents

Fatty acid hydroperoxide lyase nucleic acid sequences Download PDF

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Publication number
WO2000000627A3
WO2000000627A3 PCT/US1999/014777 US9914777W WO0000627A3 WO 2000000627 A3 WO2000000627 A3 WO 2000000627A3 US 9914777 W US9914777 W US 9914777W WO 0000627 A3 WO0000627 A3 WO 0000627A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
lyase
hpo lyase
hpo
expression
Prior art date
Application number
PCT/US1999/014777
Other languages
French (fr)
Other versions
WO2000000627A2 (en
Inventor
Kenji Matsui
Original Assignee
Calgene Llc
Kenji Matsui
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc, Kenji Matsui filed Critical Calgene Llc
Priority to EP99930829A priority Critical patent/EP1032694A2/en
Priority to KR1020007002000A priority patent/KR20010023362A/en
Priority to CA002301856A priority patent/CA2301856A1/en
Publication of WO2000000627A2 publication Critical patent/WO2000000627A2/en
Publication of WO2000000627A3 publication Critical patent/WO2000000627A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

This invention relates to plant Hydroperoxide Lyase or HPO lyase polynucleotides and polypeptides. DNA constructs useful for the expression of a plant HPO lyase in a cell are described. Furthermore, DNA constructs useful for the antisense expression of a plant HPO lyase in a plant cell are described. Such constructs will contain a DNA sequence encoding the plant HPO lyase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant HPO lyase in plant tissue, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant HPO lyase for the modification of the volatile aldehydes in plant tissues, as well as for methods of increasing disease resistance in a plant.
PCT/US1999/014777 1998-06-26 1999-06-25 Fatty acid hydroperoxide lyase nucleic acid sequences WO2000000627A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99930829A EP1032694A2 (en) 1998-06-26 1999-06-25 Fatty acid hydroperoxide lyase nucleic acid sequences
KR1020007002000A KR20010023362A (en) 1998-06-26 1999-06-25 Fatty acid hydroperoxide lyase nucleic acid sequences
CA002301856A CA2301856A1 (en) 1998-06-26 1999-06-25 Fatty acid hydroperoxide lyase nucleic acid sequences

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9092498P 1998-06-26 1998-06-26
US60/090,924 1998-06-26
US12196599P 1999-02-26 1999-02-26
US60/121,965 1999-02-26

Publications (2)

Publication Number Publication Date
WO2000000627A2 WO2000000627A2 (en) 2000-01-06
WO2000000627A3 true WO2000000627A3 (en) 2000-07-06

Family

ID=26782789

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/014777 WO2000000627A2 (en) 1998-06-26 1999-06-25 Fatty acid hydroperoxide lyase nucleic acid sequences

Country Status (5)

Country Link
EP (1) EP1032694A2 (en)
KR (1) KR20010023362A (en)
CN (1) CN1321196A (en)
CA (1) CA2301856A1 (en)
WO (1) WO2000000627A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6592619B2 (en) 1996-01-02 2003-07-15 University Of Cincinnati Heart wall actuation device for the natural heart
US6200794B1 (en) 1998-05-13 2001-03-13 Fimenrich Sa Guava (Psidium guajava) 13-hydroperoxide lyase and uses thereof
WO2000022145A2 (en) 1998-10-13 2000-04-20 Pioneer Hi-Bred International, Inc. A hydroperoxide lyase gene from maize and methods of use
WO2000050575A2 (en) * 1999-02-26 2000-08-31 Calgene Llc Nucleic acid sequence of a cucumber (cucumis sativus) fatty acid 9-hydroperoxide lyase
US6271018B1 (en) * 2000-03-29 2001-08-07 Vanderbilt University Muskmelon (Cucumis melo) hydroperoxide lyase and uses thereof
US6780621B2 (en) 2000-05-24 2004-08-24 Firmenich Sa Guava (Psidium guajava) 13-hydroperoxide lyase and uses thereof
DE10028639C2 (en) * 2000-06-09 2003-11-06 Ipk Inst Fuer Pflanzengenetik Process for the preparation of C9 aldehydes, C9 alcohols and their esters
AU2002223038A1 (en) * 2000-11-06 2002-05-15 Centre National De La Recherche Scientifique Nucleic acids and polypeptides specifically expressed in cells of the transfer zone of a plant seed and uses thereof
EP1283890A1 (en) 2000-12-18 2003-02-19 Her Majesty The Queen in Right of Canada, as represented by The Minister of Agriculture and Agri-Food Canada Hydrogen peroxide lyase regulatory region

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026364A1 (en) * 1996-01-19 1997-07-24 The Texas A & M University System Method of inhibiting mycotoxin production in seed crops by modifying lipoxygenase pathway genes
EP0801133A2 (en) * 1996-04-15 1997-10-15 Givaudan-Roure (International) S.A. Hydroperoxide lyases

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026364A1 (en) * 1996-01-19 1997-07-24 The Texas A & M University System Method of inhibiting mycotoxin production in seed crops by modifying lipoxygenase pathway genes
EP0801133A2 (en) * 1996-04-15 1997-10-15 Givaudan-Roure (International) S.A. Hydroperoxide lyases

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
BATE N J ET AL: "Molecular characterization of an Arabidopsis gene encoding hydroperoxide lyase, a cytochrome P-450 that is wound inducible.", PLANT PHYSIOLOGY, (1998 AUG) 117 (4) 1393-400., XP002128564 *
BEVAN M. ET AL.: "AC Z97339; CAB45989", EBI DATABASE, 28 June 1999 (1999-06-28) - 9 September 1999 (1999-09-09), XP002128565 *
FAUCONNIER ET AL: "Purification and characterization of tomato leaf (Lycopersicon esculentum Mill.) hydroperoxide lyase", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. WASHINGTON, vol. 45, no. 11, November 1997 (1997-11-01), pages 4232 - 4236-4236, XP002126015, ISSN: 0021-8561 *
HARMS KARSTEN ET AL: "Expression of a flax allene oxide synthase cDNA leads to increased endogenous jasmonic acid (JA) levels in transgenic potato plants but not to a corresponding activation of JA-responding genes.", PLANT CELL 1995, vol. 7, no. 10, 1995, pages 1645 - 1654, XP002136820, ISSN: 1040-4651 *
LAUDERT DIETMAR ET AL: "Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP 74), the first enzyme of the octadecanoid pathway to jasmonates.", PLANT MOLECULAR BIOLOGY 1996, vol. 31, no. 2, 1996, pages 323 - 335, XP002136822, ISSN: 0167-4412 *
MATSUI K (REPRINT): "Properties and structures of fatty acid hydroperoxide lyase", BELGIAN JOURNAL OF BOTANY, (MAR 1998) VOL. 131, NO. 1, PP. 50-62. PUBLISHER: SOC ROYAL BOTAN BELGIQUE, NIEUWELAAN 38, B-1860 MEISE, BELGIUM ISSN: 0037-9557., YAMAGUCHI UNIV, FAC AGR, DEPT BIOL CHEM, YAMAGUCHI 753, JAPAN (Reprint), XP000870239 *
MATSUI K ET AL: "Bell pepper fruit fatty acid hydroperoxide lyase is a cytochrome P450 (CYP74B).", FEBS LETTERS, (1996 SEP 23) 394 (1) 21-4., XP002126014 *
MATSUI K ET AL: "SEPARATION OF 13 AND 9 HYDROPEROXIDE LYASE ACTIVITIES IN COTYLEDONS OF CUCUMBER SEEDLINGS", ZEITSCHRIFT FUER NATURFORSCHUNG SECTION C BIOSCIENCES 1989, vol. 44, no. 9-10, 1989, pages 883 - 885, XP002136825, ISSN: 0341-0382 *
MATSUI, KENJI ET AL: "Molecular cloning and expression of Arabidopsis fatty acid hydroperoxide lyase", PLANT CELL PHYSIOL. (1999), 40(5), 477-481, XP000870044 *
MOXON, CLAIRE L. (1) ET AL: "Characterization of an Arabidopsis sequence with homology to allene oxide synthase and hydroperoxide lyase.", PLANT PHYSIOLOGY (ROCKVILLE), (1997) VOL. 114, NO. 3 SUPPL., PP. 176. MEETING INFO.: PLANT BIOLOGY '97: 1997 ANNUAL MEETINGS OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS AND THE CANADIAN SOCIETY OF PLANT PHYSIOLOGISTS, JAPANESE SOCIETY OF PLANT PHY, 1997, XP002128563 *
NEWMAN T. ET AL.: "AC T20864", EBI DATABASE, 26 June 1994 (1994-06-26), XP002128562 *
NOORDERMEER M A ET AL: "Alfalfa contains substantial 9- hydroperoxide lyase activity and a 3Z:2E-enal isomerase", FEBS LETTERS, (25 JAN 1999) VOL. 443, NO. 2, PP. 201-204. PUBLISHER: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS. ISSN: 0014-5793., UNIV UTRECHT, DEPT BIOORGAN CHEM, BIJVOET CTR BIOMOL RES, PADUALAAN 8, NL-3584 CH UTRECHT, NETHERLANDS (Reprint);UNIV UTRECHT, DEPT BIOORGAN CHEM, BIJVOET CTR BIOMOL RES, NL-3584 CH UTRECHT, NETHERLAN, XP002136824 *
PAN ZHIQIANG ET AL: "Heterologous expression and analysis of allene oxide synthase, the rubber-associated P450 from guayule.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;SAN ANTONIO, TEXAS, USA; JULY 27-31, 1996, vol. 111, no. 2 SUPPL., 1996, Plant Physiology (Rockville) 1996, pages 98, XP002136821, ISSN: 0032-0889 *
SHELDRICK B. ET AL.: "AC AJ239065", EBI DATABASE, 14 May 1999 (1999-05-14), XP002136823 *
SONG WEN-CHAO ET AL: "Molecular cloning of an allene oxide synthase: A cytochrome P450 specialized for the metabolism of fatty acid hydroperoxides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1993, vol. 90, no. 18, 1993, pages 8519 - 8523, XP002136819, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
KR20010023362A (en) 2001-03-26
CA2301856A1 (en) 2000-01-06
EP1032694A2 (en) 2000-09-06
CN1321196A (en) 2001-11-07
WO2000000627A2 (en) 2000-01-06

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