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WO2000071579A3 - Regulating organelle homeostasis in a cell factory - Google Patents

Regulating organelle homeostasis in a cell factory Download PDF

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Publication number
WO2000071579A3
WO2000071579A3 PCT/EP2000/004718 EP0004718W WO0071579A3 WO 2000071579 A3 WO2000071579 A3 WO 2000071579A3 EP 0004718 W EP0004718 W EP 0004718W WO 0071579 A3 WO0071579 A3 WO 0071579A3
Authority
WO
WIPO (PCT)
Prior art keywords
organelle
homeostasis
gene
regulating
cell factory
Prior art date
Application number
PCT/EP2000/004718
Other languages
French (fr)
Other versions
WO2000071579A2 (en
Inventor
Roelof Ary Lans Bovenberg
Den Berg Marco Alexander Van
Maarten Nieboer
Marten Veenhuis
Jan Andries Kornelis Will Kiel
Der Klei Ida Johanna Van
Original Assignee
Dsm Nv
Roelof Ary Lans Bovenberg
Den Berg Marco Alexander Van
Maarten Nieboer
Marten Veenhuis
Jan Andries Kornelis Will Kiel
Klei Ida Johanna V D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv, Roelof Ary Lans Bovenberg, Den Berg Marco Alexander Van, Maarten Nieboer, Marten Veenhuis, Jan Andries Kornelis Will Kiel, Klei Ida Johanna V D filed Critical Dsm Nv
Priority to EP00931260A priority Critical patent/EP1179071A2/en
Priority to AU49249/00A priority patent/AU4924900A/en
Publication of WO2000071579A2 publication Critical patent/WO2000071579A2/en
Publication of WO2000071579A3 publication Critical patent/WO2000071579A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/385—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Penicillium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/78—Hansenula
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/80—Penicillium
    • C12R2001/82—Penicillium chrysogenum

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method for modulating the production of secondary metabolites in the fungus Penicillium chrysogenum by influencing the homeostasis of organelles by modulating a least one gene which is directly related to organelle homeostasis in this fungus. Preferably, the organelle homeostasis is modulated by genetically engineering at least one gene encoding a protein which is responsible for organelle biogenesis (the expression and/or activity of which is to be increased) and/or at least one gene encoding a protein which is responsible for organelle degeneration (the expression and/or activity of which is to be decreased). Genes involved in organelle biosynthesis in P. chrysogenum are exemplified by the so-called pex11 gene. Genes involved in organelle degradation are exemplified by the so-called vps15 and pdd1 genes.
PCT/EP2000/004718 1999-05-21 2000-05-22 Regulating organelle homeostasis in a cell factory WO2000071579A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00931260A EP1179071A2 (en) 1999-05-21 2000-05-22 Regulating organelle homeostasis in a cell factory
AU49249/00A AU4924900A (en) 1999-05-21 2000-05-22 Regulating organelle homeostasis in a cell factory

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99201630 1999-05-21
EP99201630.3 1999-05-21

Publications (2)

Publication Number Publication Date
WO2000071579A2 WO2000071579A2 (en) 2000-11-30
WO2000071579A3 true WO2000071579A3 (en) 2001-03-01

Family

ID=8240226

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/004718 WO2000071579A2 (en) 1999-05-21 2000-05-22 Regulating organelle homeostasis in a cell factory

Country Status (3)

Country Link
EP (1) EP1179071A2 (en)
AU (1) AU4924900A (en)
WO (1) WO2000071579A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014003555A1 (en) * 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0448180A2 (en) * 1990-03-23 1991-09-25 Gist-Brocades N.V. A method of modulating the production of secondary metabolites
WO1994024289A1 (en) * 1993-04-19 1994-10-27 Eurolysine Method for addressing proteins in yeast peroxisomes
WO1997038107A1 (en) * 1996-04-04 1997-10-16 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0448180A2 (en) * 1990-03-23 1991-09-25 Gist-Brocades N.V. A method of modulating the production of secondary metabolites
WO1994024289A1 (en) * 1993-04-19 1994-10-27 Eurolysine Method for addressing proteins in yeast peroxisomes
WO1997038107A1 (en) * 1996-04-04 1997-10-16 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MARSHALL P ET AL: "Pmp27 promotes peroxisomal proliferation", J. CELL BIOL., vol. 129, no. 2, April 1995 (1995-04-01), pages 345 - 355, XP002120786 *
ROGGENKAMP R ET AL: "Formation of irregular giant peroxisomes by overproduction of the crystalloid core protein methanol oxidase in the methylotrophic yeast Hansenula polymorpha", MOL CELL BIOL., vol. 9, no. 3, March 1989 (1989-03-01), pages 988 - 994, XP000037387 *
SAKAI Y ET AL: "The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae", J BACTERIOL., vol. 177, December 1995 (1995-12-01), pages 6773 - 6781, XP002120787 *

Also Published As

Publication number Publication date
AU4924900A (en) 2000-12-12
EP1179071A2 (en) 2002-02-13
WO2000071579A2 (en) 2000-11-30

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