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WO2000071079A2 - Agents a stabilisation proteinique actifs pharmacologiquement; procedes de fabrication et methodes d'utilisation - Google Patents

Agents a stabilisation proteinique actifs pharmacologiquement; procedes de fabrication et methodes d'utilisation Download PDF

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Publication number
WO2000071079A2
WO2000071079A2 PCT/US2000/013954 US0013954W WO0071079A2 WO 2000071079 A2 WO2000071079 A2 WO 2000071079A2 US 0013954 W US0013954 W US 0013954W WO 0071079 A2 WO0071079 A2 WO 0071079A2
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WIPO (PCT)
Prior art keywords
pharmacologically active
active agent
paclitaxel
particles
composition
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PCT/US2000/013954
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English (en)
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WO2000071079A3 (fr
Inventor
Neil P. Desai
Chunlin Tao
Andrew Yang
Leslie Louie
Zhiwen Yao
Patrick Soon-Shiong
Shlomo Magdassi
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American Bioscience, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from US09/316,642 external-priority patent/US6749868B1/en
Application filed by American Bioscience, Inc. filed Critical American Bioscience, Inc.
Priority to EP00932669A priority Critical patent/EP1178786A4/fr
Priority to CA2371912A priority patent/CA2371912C/fr
Priority to AU50359/00A priority patent/AU784416B2/en
Publication of WO2000071079A2 publication Critical patent/WO2000071079A2/fr
Priority to HK02105870.2A priority patent/HK1045646A1/zh
Priority to AU2006202836A priority patent/AU2006202836B2/en
Publication of WO2000071079A3 publication Critical patent/WO2000071079A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/513—Organic macromolecular compounds; Dendrimers
    • A61K9/5169—Proteins, e.g. albumin, gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention relates to methods for the production of particulate vehicles for the intravenous administration of pharmacologically active agents, as well as novel compositions produced thereby.
  • the invention relates to methods for the in vivo delivery of substantially water insoluble pharmacologically active agents (e.g., the anticancer drug paclitaxel, the active ingredient of TaxolTM).
  • substantially water insoluble pharmacologically active agents e.g., the anticancer drug paclitaxel, the active ingredient of TaxolTM.
  • dispersible colloidal systems containing water insoluble pharmacologically active agents are provided.
  • the suspended particles are encased in a polymeric shell formulated from a biocompatible polymer, and have a diameter of less than about 1 micron.
  • Invention colloidal systems can be prepared without the use of conventional surfactant or any polymeric core matrix.
  • the invention provides a drug delivery system in either liquid form or in the form of a redispersible powder. Either form provides both immediately bioavailable drug molecules (i.e., drug molecules which are molecularly bound to a protein), and pure drug particles coated with a protein.
  • Intravenous drug delivery permits rapid and direct equilibration with the blood stream which carries the medication to the rest of the body.
  • administration of drugs carried within stable carriers would allow gradual release of the drugs inside the intravascular compartment following a bolus intravenous injection of the therapeutic nanoparticles.
  • Injectable controlled-release nanoparticles can provide a pre-programmed duration of action, ranging from days to weeks to months from a single injection. They also can offer several profound advantages over conventionally administered medicaments, including automatic assured patient compliance with the dose regimen, as well as drug targeting to specific tissues or organs (Tice and Gilley, Journal of Controlled Release 2:343-352 (1985)).
  • Microparticles and foreign bodies present in the blood are generally cleared from the circulation by the "blood filtering organs", namely the spleen, lungs and liver.
  • the particulate matter contained in normal whole blood comprises red blood cells (typically 8 microns in diameter), white blood cells (typically 6-8 microns in diameter), and platelets (typically 1-3 microns in diameter).
  • red blood cells typically 8 microns in diameter
  • white blood cells typically 6-8 microns in diameter
  • platelets typically 1-3 microns in diameter.
  • the microcirculation in most organs and tissues allows the free passage of these blood cells.
  • microthrombii blood clots
  • a suspension of particles less than 7-8 microns is, however, relatively safe and has been used for the delivery of pharmacologically active agents in the form of liposomes and emulsions, nutritional agents, and contrast media for imaging applications.
  • compositions that are water-insoluble or poorly water-soluble and sensitive to acid environments in the stomach cannot be conventionally administered (e.g., by intravenous injection or oral administration).
  • parenteral administration of such pharmaceuticals has been achieved by emulsification of the oil solubilized drug with an aqueous liquid (such as normal saline) in the presence of surfactants or emulsion stabilizers to produce stable microemulsions.
  • aqueous liquid such as normal saline
  • surfactants or emulsion stabilizers to produce stable microemulsions.
  • emulsions may be injected intravenously, provided the components of the emulsion are pharmacologically inert.
  • 4,073,943 describes the administration of water-insoluble pharmacologically active agents dissolved in oils and emulsified with water in the presence of surfactants such as egg phosphatides, pluronics (copolymers of polypropylene glycol and polyethylene glycol), polyglycerol oleate, etc.
  • surfactants such as egg phosphatides, pluronics (copolymers of polypropylene glycol and polyethylene glycol), polyglycerol oleate, etc.
  • PCT International Publication No. WO85/00011 describes pharmaceutical microdroplets of an anaesthetic coated with a phospholipid such as dimyristoyl phosphatidylcholine having suitable dimensions for intradermal or intravenous injection.
  • paclitaxel a natural product first isolated from the Pacific Yew tree, Taxus brevifolia, by Ward et al. (J. Am. Chem. Soc. 93_:2325 (1971)).
  • antimitotic agents paclitaxel, which contains a diterpene carbon skeleton, exhibits a unique mode of action on microtubule proteins responsible for the formation of the mitotic spindle.
  • paclitaxel is the only plant product known to inhibit the depolymerization process of tubulin, thus preventing the cell replication process.
  • Paclitaxel a naturally occurring diterpenoid, has been shown to have significant antineoplastic and anticancer effects in drug-refractory ovarian cancer.
  • Paclitaxel has shown excellent antitumor activity in a wide variety of tumor models such as the B 16 melanoma, L1210 leukemias, MX-1 mammary tumors, and CS-1 colon tumor xenografts.
  • TaxolTM a paclitaxel formulation, as the new anticancer wonder-drug.
  • TaxolTM has recently been approved by the Federal Drug Administration for treatment of ovarian cancer.
  • the poor aqueous solubility of paclitaxel presents a problem for human administration.
  • paclitaxel formulations e.g., TaxolTM
  • a cremaphor to solubilize the drug.
  • the human clinical dose range is 200-500 mg. This dose is dissolved in a 1 : 1 solution of ethanolxremaphor and diluted to one liter of fluid given intravenously.
  • the cremaphor currently used is polyethoxylated castor oil.
  • paclitaxel itself did not show excessive toxic effects, but severe allergic reactions were caused by the emulsifiers employed to solubilize the drug to form TaxolTM, the conventional formulation of paclitaxel.
  • the current regimen of administration involves treatment of the patient with antihistamines and steroids prior to injection of the drug to reduce the allergic side effects of the cremaphor.
  • Protein microspheres have been reported in the literature as carriers of pharmacological or diagnostic agents.
  • Microspheres of albumin have been prepared by either heat denaturation or chemical crosslinking. Heat denatured microspheres are produced from an emulsified mixture (e.g., albumin, the agent to be incorporated, and a suitable oil) at temperatures between 100°C and 150°C. The microspheres are then washed with a suitable solvent and stored. Leucuta et al. (Lnternational Journal of Pharmaceutics 41:213-217 (1988)) describe the method of preparation of heat denatured microspheres.
  • the procedure for preparing chemically crosslinked microspheres involves treating the emulsion with glutaraldehyde to crosslink the protein, followed by washing and storage.
  • Lee et al. Science 2L3_:233-235 (1981)
  • U.S. Patent No. 4,671,954 teach this method of preparation.
  • One conventional method for manufacturing drug-containing nanoparticles comprises dissolving polylactic acid (or other biocompatible, water insoluble polymers) in a water-immiscible solvent (such as methylene chloride or other chlorinated, aliphatic, or aromatic solvent), dissolving the pharmaceutically active agent in the polymer solution, adding a surfactant to the oil phase or the aqueous phase, forming an oil-in-water emulsion by suitable means, and evaporating the emulsion slowly under vacuum. If the oil droplets are sufficiently small and stable during evaporation, a suspension of the polymer in water is obtained.
  • a water-immiscible solvent such as methylene chloride or other chlorinated, aliphatic, or aromatic solvent
  • Patent 2 660 556 have reported the formation of nanoparticles by using two biocompatible polymers, where one polymer (e.g., polylactide) is dissolved in the organic phase, together with an active component such as a drug, and the other polymer, such as albumin is used as the surface active agent. After emulsification and removal of the solvent, nanoparticles are formed, in which the drug is present inside the polymeric matrix of the polylactide particles.
  • one polymer e.g., polylactide
  • an active component such as a drug
  • albumin albumin
  • polylactide the polymer commonly used in the preparation of injectable nanoparticles
  • polylactide has a surface activity which causes the rapid adsorption thereof at the dichloromethane- water interface, causing reduced interfacial tension (see, for example, Boury et al, in Langmuir ⁇ ⁇ : 1636 ( 1995)), which in turn improves the emulsification process.
  • BSA Bovine Serum Albumin
  • Another process which is based on the solvent evaporation method comprises dissolving the drug in a hydrophobic solvent (e.g., toluene or cyclohexane), without any polymer dissolved in the organic solvent, adding a conventional surfactant to the mixture as an emulsifier, forming an oil-in-water emulsion, and then evaporating the solvent to obtain dry particles of the drug (see, for example, Sjostrom et al, in J. Dispersion Science and Technology 15:89-117 (1994)). Upon removal of the nonpolar solvent, precipitation of the drug inside the solvent droplets occurs, and submicron particles are obtained.
  • a hydrophobic solvent e.g., toluene or cyclohexane
  • the size of the particles is mainly controlled by the initial size of the emulsion droplets.
  • the final particle size is reported to decrease with a decrease in the drug concentration in the organic phase. This finding is contrary to the results reported herein, wherein no conventional surfactant is used for the preparation of nanoparticles.
  • the drug used cholesteryl acetate
  • the drug used is surface active in toluene, and hence may be oriented at the oil-water interface; therefore the concentration of drug at the interface is higher, thus increasing the potential for precipitation.
  • submicron particles Formation of submicron particles has also been achieved by a precipitation process, as described by Calvo et al in J. Pharm. Sci. Var_:530 (1996).
  • the process is based on dissolving the drug (e.g., indomethacin) and the polymer (poly-caprolactone) in methylene chloride and acetone, and then pouring the solution into an aqueous phase containing a surfactant (Poloxamer 188), to yield submicron size particles (216 nm).
  • the process is performed at solvent concentrations at which no emulsion is formed.
  • taxanes e.g., paclitaxel, docetaxel, and the like
  • emulsifiers and solubilizing agents e.g., paclitaxel, docetaxel, and the like
  • substantially water insoluble pharmacologically active agents can be delivered in the form of microparticles or nanoparticles that are suitable for parenteral administration in aqueous suspension.
  • This mode of delivery obviates the necessity for administration of substantially water insoluble pharmacologically active agents (e.g., paclitaxel) in an emulsion containing, for example, ethanol and polyethoxylated castor oil, diluted in normal saline (see, for example, Norton et al, in Abstracts of the 2nd National Cancer Institute Workshop on Taxol & Taxus, September 23-24, 1992).
  • a disadvantage of such known compositions is their propensity to produce allergic side effects.
  • nanoparticles of pharmacologically active agents by a solvent evaporation technique from an oil-in-water emulsion prepared under conditions of high shear forces (e.g., sonication, high pressure homogenization, or the like), optionally without the use of any conventional surfactants and/or without the use of any polymeric core material to form the matrix of the nanoparticle.
  • proteins e.g., human serum albumin
  • stabilizing agent are employed as a stabilizing agent.
  • the invention further provides a method for the reproducible formation of unusually small nanoparticles (less than 200 nm diameter), which can be sterile-filtered through a 0.22 micron filter.
  • This is achieved by addition of a water soluble solvent (e.g., ethanol) to the organic phase and by carefully selecting the type of organic phase, the phase fraction and the drug concentration in the organic phase.
  • a water soluble solvent e.g., ethanol
  • the ability to form nanoparticles of a size that is filterable by 0.22 micron filters is of great importance and significance, since formulations which contain a significant amount of any protein (e.g., albumin), cannot be sterilized by conventional methods such as autoclaving, due to the heat coagulation of the protein.
  • compositions useful for in vivo delivery of substantially water insoluble pharmacologically active agents comprise substantially water insoluble pharmacologically active agents (as a solid or liquid) coated by an optionally crosslinkable biocompatible polymer, and optionally contained within a polymeric shell.
  • the polymeric shell is a crosslinked biocompatible polymer.
  • the polymeric shell, containing substantially water insoluble pharmacologically active agents therein, can then be suspended in a biocompatible aqueous liquid for administration.
  • the invention further provides a drug delivery system in which part of the molecules of pharmacologically active agent are bound to the protein (e.g., human serum albumin), and are therefore immediately bioavailable upon administration to a mammal.
  • the other portion of the pharmacologically active agent is contained within nanoparticles coated by protein.
  • the nanoparticles containing the pharmacologically active agent are present as a substantially pure active component, without dilution by much, if any, polymeric matrix.
  • a large number of conventional pharmacologically active agents circulate in the blood stream bound to carrier proteins (through hydrophobic or ionic interactions) of which the most common example is serum albumin.
  • Invention methods and compositions produced thereby provide for a pharmacologically active agent that is "pre-bound" to a protein (through hydrophobic or ionic interactions) prior to administration.
  • the present disclosure demonstrates both of the above-described modes of bioavailability for paclitaxel, an anticancer drug capable of binding to human serum albumin (see, for example, Kumar et al, in Research Communications in Chemical Pathology and Pharmacology 8 . 0:337 (1993)).
  • the high concentration of albumin in invention particles, compared to TaxolTM, provides a significant amount of the drug (i.e., paclitaxel) in the form of molecules bound to albumin, which is also the natural carrier of the drug in the blood stream.
  • submicron particles in powder form which can easily be reconstituted in water or saline.
  • the powder is obtained after removal of water by lyophilization.
  • Human serum albumin serves as the structural component of invention nanoparticles, and also as a cryoprotectant and reconstitution aid.
  • the preparation of particles filterable through a 0.22 micron filter according to the invention method as described herein, followed by drying or lyophilization, produces a sterile solid formulation useful for intravenous injection.
  • the invention provides, in a particular aspect, a composition of anti-cancer drugs, e.g., paclitaxel, in the form of nanoparticles in a liquid dispersion or as a solid which can be easily reconstituted for administration. Due to specific properties of certain drugs, e.g., paclitaxel, such compositions cannot be obtained by conventional solvent evaporation methods that rely on the use of surfactants. In the presence of various surfactants, very large drug crystals (e.g., size of about 5 microns to several hundred microns) are formed within a few minutes of storage, after the preparation process. The size of such crystals is typically much greater than the allowed size for intravenous injection.
  • paclitaxel Due to specific properties of certain drugs, e.g., paclitaxel, such compositions cannot be obtained by conventional solvent evaporation methods that rely on the use of surfactants. In the presence of various surfactants, very large drug crystals (e.g., size of about 5 micron
  • particles produced according to the invention can be either crystalline, amorphous, or a mixture thereof, it is generally preferred that the drug be present in the formulation in an amorphous form. This would lead to greater ease of dissolution and absorption, resulting in better bioavailability.
  • the invention further provides various methods of reducing the myelosuppressive effects and/or the neurotoxic effects of a pharmacologically active agent administered to a patient in need thereof.
  • inventions comprising administering pharmacologically active agent(s) to a patient having a disease capable of treatment by the pharmacologically active agent(s).
  • invention methods comprise administering formulations according to the invention containing suitable pharmacologically active agent(s) to the patient.
  • Diseases contemplated for treatment according to the invention include cancers, proliferative diseases, and the like.
  • invention formulations can be accomplished in a variety of ways, e.g., intravenous or intraarterial, and/or can be without the use of steroids and/or cytokines, and/or can be in combination with a biochemotherapy agent; and or the single dose levels of pharmacologically active agents can be greater than about 50 mg; and/or the cumulative dose levels of pharmacologically active agents can be greater than about 250 mg/m 2 every 3 weeks.
  • inventions for delivering a pharmacologically active agent to a localized area of a patient for sustained release of the pharmacologically active agent over an extended period of time (e.g., from about 1 day to about 1 year).
  • Invention methods comprise administering to the patient a suitable pharmacologically active agent in the invention formulation, wherein the invention formulation has been dispersed within a matrix of suitable biocompatible material.
  • methods of orally administering pharmacologically active agent(s) to a patient in need thereof comprise orally administering an invention formulation of the pharmacologically active agent(s) in combination with intestinal cell efflux inhibitor(s).
  • inventions for administering a combination of suitable pharmacologically active agent(s) to a patient in need thereof.
  • invention methods comprise administering to the patient 25-75% of the conventionally effective dosage level of each of the suitable pharmacologically active agent(s) in the invention formulation.
  • Dose for the treatment group is 20 mg/kg of paclitaxel administered as an intravenous bolus for five consecutive days.
  • Figure 2 presents the results of intraperitoneal administration of paclitaxel nanoparticles in rats that have developed arthritis in their paws following intradermal injection of collagen. Paw volumes are measured and indicate the severity of the disease. The paw volumes are normalized to 100% at the beginning of treatment. Day 0 represents the initiation of treatment.
  • the two treatment groups show a dramatic reduction in paw volume with time, indicating a regression of arthritis, while the control group showed an increase in paw volume over the same period.
  • a method for the preparation of substantially water insoluble pharmacologically active agents for in vivo delivery comprising subjecting a mixture comprising: an organic phase containing said pharmacologically active agent dispersed therein, and aqueous medium containing biocompatible polymer, wherein said mixture optionally contains substantially no surfactants, in a high pressure homogenizer to a predetermined pressure.
  • This predetermined pressure can be in the range of about 100 up to about 100,000 psi, and preferably in the range of about 2,000 up to about 60,000 psi, and can be in a presently preferred range of about 3,000 to about 40,000 psi.
  • such processes can be carried out at a predetermined pressure in the range of about 3,000 psi up to about
  • the organic and/or aqueous phases are thereafter removed from the mixture after having been subjected to high shear conditions.
  • compositions prepared by the above-described method are compositions prepared by the above-described method.
  • a drug delivery system comprising particles of a solid or liquid, substantially water insoluble pharmacologically active agent, coated with a protein,
  • compositions are particularly advantageous as they have been observed to provide a very low toxicity form of a variety of pharmacologically active agents, e.g., the combination of paclitaxel and albumin (as the biocompatible polymer) is a presently preferred combination because of its low toxicity.
  • the combination of paclitaxel and albumin also has the added advantage of being substantially non-myelosuppressive.
  • the average diameter of the above-described particles is no greater than about 200 nm.
  • Such particles are particularly advantageous as they can be subjected to sterile filtration, thereby obviating the need for more vigorous treatment to achieve sterilization of solutions containing the desired pharmacologically active agent.
  • exemplary routes of administration include topical, oral, intraarticular, intracistemal, intraocular, intraventricular, intrathecal, intravenous, intramuscular, intraperitoneal, intradermal/transdermal subcutaneous, intratracheal inhalational, rectal (i.e., via suppository), vaginal (i.e., via pessary), intracranial, intraurethral, intrahepatic, intraarterial, intratumoral, mucosal, and the like, as well as suitable combinations of any two or more thereof.
  • administration of the pharmacologically active agent contemplated for use in the present invention can be systemic (i.e., administered to the subject as a whole via any of the above routes) or localized (i.e., administered to the specific location of the particular infirmity of the subject via any of the above routes).
  • Exemplary means for the systemic administration of pharmacologically active agent(s) are well known to those of skill in the art, and include oral (for example, with a sustained release formulation of the pharmacologically active agent), continuous IV infusion, infusion via bolus injection, infusion through in-dwelling catheters, and any other means which can function to deliver the pharmacologically active agent systemically to the patient in need thereof, and the like, and suitable combinations of any two or more thereof.
  • Exemplary means for the localized administration of pharmacologically active agent(s) include catheters, implantable or portable infusion devices, slow release delivery vehicles, and any other means which can function to deliver the pharmacologically active agent to the localized area of the infirmity to be treated, and the like, and suitable combinations of any two or more thereof.
  • Implantable or portable infusion devices contemplated for use in the present invention are well known to those of skill in the art, and include devices which can deliver precise and controlled amounts of the pharmacologically active agent over extended periods. Typically, these are driven by electromagnetic force, and/or osmotic force, and or hydrostatic force, and or gaseous pressure, and/or mechanical force. Commonly, implantable infusion devices are capable of being periodically refilled, and of being able to receive the pharmacologically active agent in solid or liquid form.
  • Exemplary slow release delivery vehicles include, for example, pharmacologically active agent(s) encapsulated in a colloidal dispersion system or in a polymer stabilized system.
  • Useful colloidal dispersion systems include nanocapsules, microspheres, beads, lipid-based systems (including oil-in-water emulsions, micelles, mixed micelles, liposomes, and the like), and the like.
  • the colloidal system presently preferred is a liposome or microsphere.
  • Liposomes are artificial membrane vesicles which are useful as slow release delivery vehicles when injected or implanted.
  • slow release delivery vehicles are biodegradable hydrogel matrices (U.S. Patent No. 5,041, 292), dendritic polymer conjugates (U.S. Patent No. 5,714,166), and multivesicular liposomes (Depofoam ® , Depotech, San Diego, CA) (U. S. Patent Nos. 5,723,147 and 5,766,627).
  • One type of microspheres suitable for encapsulating therapeutic agents for local injection is poly(D,L)lactide microspheres, as described in D. Fletcher, Anesth. Analg. 84:90-94, 1997.
  • localized administration Besides delivering an effective therapeutic dose to the site of the infirmity and decreasing the chance of systemic toxicity, localized administration also decreases the exposure of the pharmacologically active agent to degradative processes, such as proteolytic degradation and immunological intervention via antigenic and immunogenic responses, as well as to systemic clearance processes, such as sequestration in the liver.
  • biocompatible describes a substance that does not appreciably alter or affect in any adverse way, the biological system into which it is introduced.
  • the stabilizing agent e.g., a protein
  • the stabilizing agent can optionally be crosslinked as a result of exposure to high shear conditions in a high pressure homogenizer.
  • High shear is used to disperse a dispersing agent containing dissolved or suspended pharmacologically active agent into an aqueous solution of a biocompatible polymer, optionally bearing sulfhydryl or disulfide groups (e.g., albumin) whereby a coating of stabilizing agent (or, optionally, a shell of crosslinked polymer) is formed around fine droplets of non-aqueous medium.
  • a biocompatible polymer optionally bearing sulfhydryl or disulfide groups (e.g., albumin)
  • stabilizing agent or, optionally, a shell of crosslinked polymer
  • the high shear conditions produce cavitation in the liquid that causes tremendous local heating and results in the formation of superoxide ions that are capable of crosslinking the polymer, for example, by oxidizing the sulfhydryl residues (and/or disrupting existing disulfide bonds) to form new, crosslinking disulfide bonds.
  • the prior art method of glutaraldehyde crosslinking is nonspecific and essentially reactive with any nucleophilic group present in the protein structure (e.g., amines and hydroxyls).
  • Heat denaturation as taught by the prior art significantly and irreversibly alters protein structure.
  • disulfide formation contemplated by the present invention does not substantially denature the protein.
  • particles of substantially water insoluble pharmacologically active agents coated with a stabilizing agent (and/or optionally contained within a shell) differ from crosslinked or heat denatured protein microspheres of the prior art because the coating (and/or polymeric shell) produced by the invention process is relatively thin compared to the diameter of the coated particle.
  • the "shell thickness" of the polymeric coat is approximately 25 nanometers for a coated particle having a diameter of 1 micron (1000 nanometers).
  • microspheres of the prior art do not have protein shells, but rather, have protein dispersed throughout the volume of the microsphere.
  • micron refers to a unit of measure of one one- thousandth of a millimeter.
  • a pharmacologically active agent is dissolved in a suitable solvent.
  • a protein e.g., human serum albumin, other suitable polymers listed below, and the like
  • a stabilizing agent for the formation of stable nanodroplets.
  • polymer need not be dissolved in the mixture.
  • Polymer can desirably be added to the mixture, however, when additional control over the nanoparticle size is warranted.
  • exemplary polymers include polylactic/polyglycolic acids and copolymers, polyvinyl alcohol, polyvinyl acetate, polyesters, and other synthetic and natural polymers functional to aid in control of the nanoparticle size, and the like, and suitable combinations of any two or more thereof.
  • surfactant need not be added to the mixture.
  • Surfactant can desirably be added to the mixture, however, when additional control over solvation of the pharmacologically active agent is warranted.
  • exemplary surfactants include sodium lauryl sulfate, lecithin, Spans, Tweens (e.g., tween 80, and the like), block copolymers (e.g., pluronics (e.g., pluronic F-68, and the like), tetronics, and the like), and other pharmaceutically acceptable surfactants, and suitable combinations of any two or more thereof.
  • foam suppressant need not be added to the mixture.
  • Foam suppressant can desirably be added to the mixture, however, when additional control over the suppression of foam in the formation of the nanoparticles is warranted.
  • exemplary foam suppressants include silicones, oils, hydrocarbons, alcohols, other compounds which function to suppress foaming in the formation of the nanoparticles, and the like, and suitable combinations of any two or more thereof.
  • the oil phase employed in the preparation of invention compositions typically contains only the pharmacologically active agent dissolved in solvent
  • the aqueous phase employed in the preparation of invention compositions commonly contains only the protein dissolved in aqueous medium.
  • an emulsion is formed by homogenization under high pressure and high shear forces.
  • Such homogenization is conveniently carried out in a high pressure homogenizer, typically operated at pressures in the range of about 100 up to about 100,000 psi, and preferably in the range of about 2,000 up to 60,000 psi, and can be in a presently preferred range of about 3,000 to about 40,000 psi.
  • a predetermined pressure in the range of about 3,000 psi up to about 30,000 psi.
  • such processes are carried out at pressures in the range of about 6,000 up to 25,000 psi, and even as high as 40,000 psi.
  • the resulting emulsion comprises very small nanodroplets of the nonaqueous solvent (containing the dissolved pharmacologically active agent) and very small nanodroplets of the protein stabilizing agent.
  • Acceptable methods of homogenization include processes imparting high shear and cavitation such as high pressure homogenization, high shear mixers, sonication, high shear impellers, and the like.
  • Processes imparting shear and cavitation forces accomplish high pressure homogenization by using devices such as sonicators, homogenizers, mixers, impellers, and the like (e.g., devices commercially available from such sources as Heat Systems, Microfluidics, Avestin, Stansted, APV, Gaulin, Rannie, Ross, Silverson, Niro, and the like), and suitable combinations of any two or more thereof.
  • devices such as sonicators, homogenizers, mixers, impellers, and the like (e.g., devices commercially available from such sources as Heat Systems, Microfluidics, Avestin, Stansted, APV, Gaulin, Rannie, Ross, Silverson, Niro, and the like), and suitable combinations of any two or more thereof.
  • the product passes through an interaction chamber or a homogenizing valve which channels the product through narrow orifices with tortuous paths
  • the product can also be extruded under pressure through membranes or other devices having small pores whose size is in the range from about 0.025 micron to about several (e.g., up to about 200) microns.
  • the solvent is evaporated under reduced pressure to yield a colloidal system composed of protein coated nanoparticles of pharmacologically active agent and protein.
  • a wide variety of methods of evaporation are suitable for use in the practice of the present invention, including using device(s) selected from rotary evaporators, film evaporators, rising film evaporators, falling film evaporators, agitated film evaporators (e.g., Rototherm), concentrators, evaporator/strippers, multistage evaporators, spray driers, lyophilizers, flash evaporators, freeze driers, or combinations of different types of evaporators such as those available from Buchi, LCI, Artisan, Pope, and Niro, or the like, or suitable combinations of any two or more thereof.
  • the colloidal system produced upon evaporation of the solvent can be ultrafiltered for further concentration or to remove small molecules (e.g., organics, salts, contaminants, and the like).
  • this ultrafiltration can be accomplished by a variety of methodologies adaptable to the practice of the present invention, e.g., by using ultrafiltration device(s) such as those commercially available from Sartorius, Millipore, Pall, and the like.
  • This ultrafiltration can be conducted prior to, in between, or after the optional filtration(s) identified in the succeeding paragraph, e.g., prior to conventional filtration, in between the stages of prefiltration and sterile filtration or after sterile filtration.
  • the colloidal system produced upon evaporation of the solvent can be conventionally filtered and/or sterilized by filtration through sterilizing filter(s) (e.g., sterilizing filters such as membrane filters, track etched filters, depth filters and the like, and suitable combinations of any two or more thereof).
  • sterilizing filters are commercially available from Sartorius, Millipore, Gelman, Pall, Nuclepore, and the like. Where prefiltration is desirable, prefilter(s) can be utilized prior to sterile filtration.
  • the entire process of manufacture of the product e.g., the preparation of the mixture, and/or the formation of the emulsion by homogenization, and/or the formation of the colloidal system by evaporation of the solvent, and or the ultrafiltration, and/or the sterile filtration, as applicable
  • the entire process of manufacture of the product can be conducted in a batchwise mode or in a continuous mode or by a combination of batch and continuous processes.
  • the homogenizer equipment mentioned above can be operated in a number of different ways, e.g., utilizing batch processes, continuous processes or a combination of batch and continuous processes.
  • this homogenizer equipment can be operated in the recycle mode with continuous recycling until the product meets the required particle size, and/or with discrete cycling (i.e., all of the product is processed for a fixed number of cycles (passes)), and/or in a continuous mode with recycle while removing a fixed percentage of the recycled product continuously.
  • multiple units of the homogenizer equipment can be connected in series to achieve the desired quality for the product.
  • the evaporator equipment can be operated in batch mode, continuous mode or by a combination of batch and continuous processes.
  • continuous mode evaporation the product can be processed once through, or can be recycled continuously through the evaporator until such time as the desired quality of product is attained.
  • batch mode evaporation the product may be processed once through the evaporator, provided the desired quality of product is achieved.
  • the liquid suspension may be dried to obtain a powder containing the pharmacologically active agent and protein.
  • the resulting powder can be redispersed at any convenient time into a suitable aqueous medium such as saline, buffered saline, water, buffered aqueous media, solutions of amino acids, solutions of vitamins, solutions of carbohydrates, or the like, as well as combinations of any two or more thereof, to obtain a suspension that can be administered to mammals.
  • a suitable aqueous medium such as saline, buffered saline, water, buffered aqueous media, solutions of amino acids, solutions of vitamins, solutions of carbohydrates, or the like, as well as combinations of any two or more thereof.
  • Methods contemplated for obtaining this powder include freeze-drying, spray drying, and the like.
  • nanoparticles i.e., particles which are less than 200 nanometers in diameter.
  • Such particles are capable of being sterile-filtered before use in the form of a liquid suspension.
  • the ability to sterile-filter the end product of the invention formulation process i.e., the drug particles
  • the ability to sterile-filter the end product of the invention formulation process is of great importance since it is impossible to sterilize dispersions which contain high concentrations of protein (e.g., serum albumin) by conventional means such as autoclaving.
  • protein e.g., serum albumin
  • pharmacologically active agent(s) is initially dissolved in a substantially water immiscible organic solvent (e.g., a solvent having less than about 5% solubility in water, such as, for example, chloroform, and other suitable solvents and organic solvents as described below) at high concentration, thereby forming an oil phase containing the pharmacologically active agent(s).
  • a substantially water immiscible organic solvent e.g., a solvent having less than about 5% solubility in water, such as, for example, chloroform, and other suitable solvents and organic solvents as described below
  • the oil phase employed in the process of the present invention generally contains only the pharmacologically active agent(s) dissolved in solvent.
  • a water miscible organic solvent e.g., a solvent having greater than about 10% solubility in water, such as, for example, ethanol
  • a water miscible organic solvent is optionally added to the oil phase at a final concentration in the range of about 1% - 99% v/v, more preferably in the range of about 5% - 25% v/v of the total organic phase.
  • the water miscible organic solvent can be selected from such solvents as ethyl acetate, ethanol, tetrahydrofuran, dioxane, acetonitrile, acetone, dimethyl sulfoxide, dimethyl formamide, methyl pyrrolidinone, and the like, and other suitable solvents and organic media as described below.
  • the mixture of water immiscible solvent with the water miscible solvent is prepared first, followed by dissolution of the pharmacologically active agent(s) in the mixture.
  • human serum albumin or any other suitable stabilizing agent as described herein is dissolved in aqueous media.
  • This component acts as a stabilizing agent for the formation of stable nanodroplets.
  • a sufficient amount of the first organic solvent i.e., the substantially water immiscible organic solvent discussed above, e.g.., chloroform
  • a separate, measured amount of the total organic phase is added to the saturated aqueous phase, so that the phase fraction of the organic phase is between about 0.1% - 50% v/v, and more preferably between 1% and 15% v/v.
  • polymer(s) and/or surfactant(s) and/or foam suppressant(s) need not be added to the mixture, although such surfactant(s) and or foam suppressant(s) can be added when additional control over the nanoparticle size, and/or additional control over solvation of the pharmacologically active agent, and/or over the suppression of foam in the formation of the nanoparticle, respectively, is desirable.
  • a mixture composed of micro and nanodroplets is formed by homogenization at low shear forces. This can be accomplished in a variety of ways, as can readily be identified by those of skill in the art, employing, for example, a conventional laboratory homogenizer operated in the range of about 2,000 up to about 15,000 ⁇ m.
  • homogenization under high pressure i.e., in the range of about 100 up to about 100,000 psi, and preferably in the range of about 2,000 up to about 60,000 psi, and can be in a presently preferred range of about 3,000 to about 40,000 psi).
  • high pressure homogenization can be carried out at a predetermined pressure in the range of about 3,000 psi up to about 30,000 psi.
  • the resulting mixture comprises an aqueous protein solution (e.g., human serum albumin), the water insoluble pharmacologically active agent, and the organic solvent(s).
  • colloidal dispersion system pharmaceutically active agent and protein
  • solvent is rapidly evaporated under vacuum to yield a colloidal dispersion system (pharmacologically active agent and protein) in the form of extremely small nanoparticles (i.e., particles in the range of about lOnm - 200 nm diameter), and thus can be sterile-filtered, and optionally conventionally filtered and/or ultra- filtered.
  • the preferred size range of the particles is between about 50 nm - 170 nm, depending on the formulation and operational parameters.
  • Colloidal systems prepared in accordance with the present invention may be further converted into powder form by removal of the water therefrom, e.g., by lyophilization at a suitable temperature-time profile.
  • other conventional modes of water removal e.g., spray drying
  • the protein e.g., human serum albumin
  • the protein itself acts as a cryoprotectant, and the powder is easily reconstituted by addition of water, saline or buffer, without the need to use such conventional cryoprotectants as mannitol, sucrose, glycine, and the like. While not required, it is of course understood that conventional cryoprotectants may be added to invention formulations if so desired.
  • the coating on the pharmacologically active agent(s) allows for the delivery of high doses of the pharmacologically active agent in relatively small volumes. This minimizes patient discomfort at receiving large volumes of fluid and minimizes hospital stay.
  • the walls of the polymeric shell or coating are generally completely degradable in vivo by proteolytic enzymes (e.g., when the polymer is a protein), resulting in no side effects from the delivery system as is the case with current formulations.
  • particles of substantially water insoluble pharmacologically active agents have a cross-sectional diameter of no greater than about 10 microns.
  • a cross-sectional diameter of less than 5 microns is more preferred, while a cross-sectional diameter of less than 1 micron is presently the most preferred for the intravenous route of administration.
  • Substantially water insoluble pharmacologically active agents contemplated for use in the practice of the present invention include pharmaceutically active agents, diagnostic agents, agents of nutritional value, and the like.
  • pharmaceutically active agents include:
  • analgesics/antipyretics e.g., aspirin, acetaminophen, ibuprofen, naproxen sodium, bupreno ⁇ hine hydrochloride, propoxyphene hydrochloride, propoxyphene napsylate, meperidine hydrochloride, hydromo ⁇ hone hydrochloride, mo ⁇ hine sulfate, oxycodone hydrochloride, codeine phosphate, dihydrocodeine bitartrate, pentazocine hydrochloride, hydrocodone bitartrate, levo ⁇ hanol tartrate, diflunisal, trolamine salicylate, nalbuphine hydrochloride, mefenamic acid, buto ⁇ hanol tartrate, choline salicylate, butalbital, phenyltoloxamine citrate, diphenhydramine citrate, methotrimeprazine, cinnamedrine hydrochloride, meprobamate, and the like);
  • anesthetics e.g., cyclopropane, enflurane, halothane, isoflurane, methoxyflurane, nitrous oxide, propofol, and the like;
  • antiasthmatics e.g., Azelastine, Ketotifen, Traxanox, and the like
  • Azelastine e.g., Azelastine, Ketotifen, Traxanox, and the like
  • antibiotics e.g., neomycin, streptomycin, chloramphenicol, cephalosporin, ampicillin, penicillin, tetracycline, and the like
  • antidepressants e.g., nefopam, oxypertine, doxepin hydrochloride, amoxapine, trazodone hydrochloride, amitriptyline hydrochloride, maprotiline hydrochloride, phenelzine sulfate, desipramine hydrochloride, nortriptyline hydrochloride, tranylcypromine sulfate, fluoxetine hydrochloride, doxepin hydrochloride, imipramine hydrochloride, imipramine pamoate, nortriptyline, amitriptyline hydrochloride, isocarboxazid, desipramine hydrochloride, trimipramine maleate, protriptyline hydrochloride, and the like);
  • antidiabetics e.g., biguanides, hormones, sulfonylurea derivatives, and the like
  • biguanides e.g., biguanides, hormones, sulfonylurea derivatives, and the like
  • antifungal agents e.g., griseofulvin, keloconazole, amphotericin B, Nystatin, candicidin, and the like
  • griseofulvin e.g., griseofulvin, keloconazole, amphotericin B, Nystatin, candicidin, and the like
  • antihypertensive agents e.g., propanolol, propafenone, oxyprenolol, Nifedipine, rese ⁇ ine, trimethaphan camsylate, phenoxybenzamine hydrochloride, pargyline hydrochloride, dese ⁇ idine, diazoxide, guanethidine monosulfate, minoxidil, rescinnamine, sodium nitroprusside, rauwolfia se ⁇ entina, alseroxylon, phentolamine mesylate, rese ⁇ ine, and the like);
  • antihypertensive agents e.g., propanolol, propafenone, oxyprenolol, Nifedipine, rese ⁇ ine, trimethaphan camsylate, phenoxybenzamine hydrochloride, pargyline hydrochloride, dese ⁇ idine, diazoxide, guanethidine monosul
  • anti-inflammatories e.g., (non-steroidal) indomethacin, naproxen, ibuprofen, ramifenazone, piroxicam, (steroidal) cortisone, dexamethasone, fluazacort, hydrocortisone, prednisolone, prednisone, and the like);
  • antineoplastics e.g., adriamycin, cyclophosphamide, actinomycin, bleomycin, duanorubicin, doxorubicin, epirubicin, mitomycin, methotrexate, fluorouracil, carboplatin, carmustine (BCNU), methyl-CCNU, cisplatin, etoposide, interferons, camptothecin and derivatives thereof, phenesterine, taxanes and derivatives thereof (e.g., paclitaxel and derivatives thereof, taxotere and derivatives thereof, and the like), vinblastine, vincristine, tamoxifen, piposulfan, and the like);
  • antineoplastics e.g., adriamycin, cyclophosphamide, actinomycin, bleomycin, duanorubicin, doxorubicin, epirubicin, mitomycin, methotrexate, flu
  • antianxiety agents e.g., lorazepam, buspirone hydrochloride, prazepam, chlordiazepoxide hydrochloride, oxazepam, clorazepate dipotassium, diazepam, hydroxyzine pamoate, hydroxyzine hydrochloride, alprazolam, droperidol, halazepam, chlormezanone, dantrolene, and the like);
  • antianxiety agents e.g., lorazepam, buspirone hydrochloride, prazepam, chlordiazepoxide hydrochloride, oxazepam, clorazepate dipotassium, diazepam, hydroxyzine pamoate, hydroxyzine hydrochloride, alprazolam, droperidol, halazepam, chlormezanone, dantrolene, and the like);
  • immunosuppressive agents e.g., cyclosporine, azathioprine, mizoribine, FK506 (tacrolimus), and the like
  • immunosuppressive agents e.g., cyclosporine, azathioprine, mizoribine, FK506 (tacrolimus), and the like
  • antimigraine agents e.g., ergotamine tartrate, propanolol hydrochloride, isometheptene mucate, dichloralphenazone, and the like
  • antimigraine agents e.g., ergotamine tartrate, propanolol hydrochloride, isometheptene mucate, dichloralphenazone, and the like
  • sedatives/hypnotics e.g., barbiturates (e.g., pentobarbital, pentobarbital sodium, secobarbital sodium, and the like), benzodiazapines (e.g., flurazepam hydrochloride, triazolam, tomazeparm, midazolam hydrochloride, and the like), and the like);
  • antianginal agents e.g., beta-adrenergic blockers, calcium channel blockers (e.g., nifedipine, diltiazem hydrochloride, and the like), nitrates (e.g., nitroglycerin, isosorbide dinitrate, pentaerythritol tetranitrate, erythriryl tetranitrate, and the like), and the like);
  • beta-adrenergic blockers e.g., calcium channel blockers (e.g., nifedipine, diltiazem hydrochloride, and the like)
  • nitrates e.g., nitroglycerin, isosorbide dinitrate, pentaerythritol tetranitrate, erythriryl tetranitrate, and the like
  • antipsychotic agents e.g., haloperidol, loxapine succinate, loxapine hydrochloride, thioridazine, thioridazine hydrochloride, thiothixene, fluphenazine hydrochloride, fluphenazine decanoate, fluphenazine enanthate, trifluoperazine hydrochloride, chlo ⁇ romazine hydrochloride, pe ⁇ henazine, lithium citrate, prochlo ⁇ erazine, and the like);
  • antipsychotic agents e.g., haloperidol, loxapine succinate, loxapine hydrochloride, thioridazine, thioridazine hydrochloride, thiothixene, fluphenazine hydrochloride, fluphenazine decanoate, fluphenazine enanthate, trifluoperazine hydrochloride, chlo ⁇ romazin
  • antimanic agents e.g., lithium carbonate, and the like.
  • antiarrhythmics e.g., bretylium tosylate, esmolol hydrochloride, verapamil hydrochloride, amiodarone, encainide hydrochloride, digoxin, digitoxin, mexiletine hydrochloride, disopyramide phosphate, procainamide hydrochloride, quinidine sulfate, quinidine gluconate, quinidine polygalacturonate, flecainide acetate, tocainide hydrochloride, lidocaine hydrochloride, and the like); antiarthritic agents (e.g., phenylbutazone, sulindac, penicillamine, salsalate, piroxicam, azathioprine, indomethacin, meclofenamate sodium, gold sodium thiomalate, ketoprofen, auranofin, aurothioglucose, tolmetin sodium, and the like);
  • antiarrhythmics
  • antigout agents e.g., colchicine, allopurinol, and the like.
  • anticoagulants e.g., heparin, heparin sodium, warfarin sodium, and the like
  • anticoagulants e.g., heparin, heparin sodium, warfarin sodium, and the like
  • thrombolytic agents e.g., urokinase, streptokinase, altoplase, and the like
  • thrombolytic agents e.g., urokinase, streptokinase, altoplase, and the like
  • antifibrinolytic agents e.g., aminocaproic acid, and the like.
  • hemorheologic agents e.g., pentoxifylline, and the like.
  • antiplatelet agents e.g., aspirin, empirin, ascriptin, and the like
  • anticonvulsants e.g., valproic acid, divalproate sodium, phenytoin, phenytoin sodium, clonazepam, primidone, phenobarbitol, phenobarbitol sodium, carbamazepine, amobarbital sodium, methsuximide, metharbital, mephobarbital, mephenytoin, phensuximide, paramethadione, ethotoin, phenacemide, secobarbitol sodium, clorazepate dipotassium, trimethadione, and the like);
  • antiparkinson agents e.g., ethosuximide, and the like
  • antihistamines/antipruritics e.g., hydroxyzine hydrochloride, diphenhydramine hydrochloride, chlo ⁇ heniramine maleate, brompheniramine maleate, cyproheptadine hydrochloride, terfenadine, clemastine fumarate, triprolidine hydrochloride, carbmoxamine maleate, diphenylpyraline hydrochloride, phenindamine tartrate, azatadine maleate, tripelennamine hydrochloride, dexchlo ⁇ heniramine maleate, methdilazine hydrochloride, trimprazine tartrate, and the like);
  • agents useful for calcium regulation e.g., calcitonin, parathyroid hormone, and the like
  • antibacterial agents e.g., a ikacin sulfate, aztreonam, chloramphenicol, chloramphenicol palmitate, chloramphenicol sodium succinate, ciprofloxacin hydrochloride, clindamycin hydrochloride, clindamycin palmitate, clindamycin phosphate, metronidazole, metronidazole hydrochloride, gentamicin sulfate, lincomycin hydrochloride, tobramycin sulfate, vancomycin hydrochloride, polymyxin B sulfate, colistimethate sodium, colistin sulfate, and the like);
  • antibacterial agents e.g., a ikacin sulfate, aztreonam, chloramphenicol, chloramphenicol palmitate, chloramphenicol sodium succinate, ciprofloxacin hydrochloride, clindamycin hydrochloride, clind
  • antiviral agents e.g., interferon gamma, zidovudine, amantadine hydrochloride, ribavirin, acyclovir, and the like;
  • antimicrobials e.g., cephalosporins (e.g., cefazolin sodium, cephradine, cefaclor, cephapirin sodium, ceftizoxime sodium, cefoperazone sodium, cefotetan disodium, cefutoxime azotil, cefotaxime sodium, cefadroxil monohydrate, ceftazidime, cephalexin, cephalothin sodium, cephalexin hydrochloride monohydrate, cefamandole nafate, cefoxitin sodium, cefonicid sodium, ceforanide, ceftriaxone sodium, ceftazidime, cefadroxil, cephradine, cefuroxime sodium, and the like), penicillins (e.g., ampicillin, amoxicillin, penicillin G benzathine, cyclacillin, ampicillin sodium, penicillin G potassium, penicillin V potassium, piperacillin sodium, oxacillin
  • anti-infectives e.g., GM-CSF, and the like
  • bronchodialators e.g., sympathomimetics (e.g., epinephrine hydrochloride, metaproterenol sulfate, terbutaline sulfate, isoetharine, isoetharine mesylate, isoetharine hydrochloride, albuterol sulfate, albuterol, bitolterol, mesylate isoproterenol hydrochloride, terbutaline sulfate, epinephrine bitartrate, metaproterenol sulfate, epinephrine, epinephrine bitartrate, and the like), anticholinergic agents (e.g., ipratropium bromide, and the like), xanthines (e.g., aminophylline, dyphylline, metaproterenol sulfate, aminophylline, and the like), mast cell stabilizers (e
  • hormones e.g., androgens (e.g., danazol, testosterone cypionate, fluoxymesterone, ethyltostosterone, testosterone enanihate, methyltestosterone, fluoxymesterone, testosterone cypionate,and the like), estrogens (e.g., estradiol, estropipate, conjugated estrogens, and the like), progestins (e.g., methoxyprogesterone acetate, norethindrone acetate, and the like), corticosteroids (e.g., triamcinolone, betamethasone, betamethasone sodium phosphate, dexamethasone, dexamethasone sodium phosphate, dexamethasone acetate, prednisone, methylprednisolone acetate suspension, triamcinolone acetonide, methylprednisolone, prednisolone sodium phosphate methylpred
  • hypoglycemic agents e.g., human insulin, purified beef insulin, purified pork insulin, glyburide, chlo ⁇ ropamide, glipizide, tolbutamide, tolazamide, and the like;
  • hypolipidemic agents e.g., clofibrate, dexfrothyroxine sodium, probucol, lovastatin, niacin, and the like
  • hypolipidemic agents e.g., clofibrate, dexfrothyroxine sodium, probucol, lovastatin, niacin, and the like
  • proteins e.g., DNase, alginase, superoxide dismutase, lipase, and the like
  • nucleic acids e.g., sense or anti-sense nucleic acids encoding any therapeutically useful protein, including any of the proteins described herein, and the like
  • agents useful for erythropoiesis stimulation e.g., erythropoietin, and the like
  • antiulcer/antireflux agents e.g., famotidine, cimetidine, ranitidine hydrochloride, and the like
  • famotidine e.g., famotidine, cimetidine, ranitidine hydrochloride, and the like
  • antinauseants/antiemetics e.g., meclizine hydrochloride, nabilone, prochlo ⁇ erazine, dimenhydrinate, promethazine hydrochloride, thiethylperazine, scopolamine, and the like;
  • oil-soluble vitamins e.g., vitamins A, D, E, K, and the like
  • compositions which are substantially water insoluble include those compounds which are substantially water insoluble and which are listed in the "Therapeutic Category and Biological Activity Index” of The Merck Index (12th Ed'n, 1996), the entire relevant contents of which are hereby inco ⁇ orated by reference.
  • diagnostic agents contemplated for use in the practice of the present invention include ultrasound contrast agents, radiocontrast agents (e.g., iodo-octanes, halocarbons, renografin, and the like), magnetic contrast agents (e.g., fluorocarbons, lipid soluble paramagnetic compounds, and the like), as well as other diagnostic agents which cannot readily be delivered without some physical and/or chemical modification to accommodate the substantially water insoluble nature thereof.
  • radiocontrast agents e.g., iodo-octanes, halocarbons, renografin, and the like
  • magnetic contrast agents e.g., fluorocarbons, lipid soluble paramagnetic compounds, and the like
  • agents of nutritional value contemplated for use in the practice of the present invention include amino acids, sugars, proteins, carbohydrates, fat-soluble vitamins (e.g., vitamins A, D, E, K, and the like) or fat, or combinations of any two or more thereof.
  • Pharmacologically active agent(s) can be present in a broad range of concentrations in the invention composition, as determined by the end use application of the invention composition. For example, pharmacologically active agent(s) can be present in the invention composition in a range from about 0.0001 % w/v to about 90 % w/v, as measured in the final mixture prior to evaporation and lyophilization.
  • the concentration of the pharmacologically active agent in the organic solvent(s) can be in the range from about 0.001 mg/ml to about 1000 mg/ml.
  • a number of biocompatible polymers may be employed in the practice of the present invention for the formation of the stabilizing agent which coats (and, optionally, for the formation of the polymeric shell which surrounds) the substantially water insoluble pharmacologically active agents.
  • any polymer, natural or synthetic, optionally bearing sulfhydryl groups or disulfide bonds within its structure may be utilized for the preparation of a coating (e.g., a disulfide crosslinked shell, and the like) about particles of substantially water insoluble pharmacologically active agents.
  • the optional sulfhydryl groups or disulfide linkages may be preexisting within the polymer structure or they may be introduced by a suitable chemical modification.
  • natural polymers such as proteins, peptides, polynucleic acids, polysaccharides (e.g., starch, cellulose, dextrans, alginates, chitosan, pectin, hyaluronic acid, and the like), proteoglycans, lipoproteins, and so on, are candidates for such modification.
  • polysaccharides e.g., starch, cellulose, dextrans, alginates, chitosan, pectin, hyaluronic acid, and the like
  • proteoglycans e.g., lipoproteins, and so on
  • Proteins contemplated for use as stabilizing agents in accordance with the present invention include albumins (which contain 35 cysteine residues), immunoglobulins, caseins, insulins (which contain 6 cysteines), hemoglobins (which contain 6 cysteine residues per a ⁇ unit), lysozymes (which contain 8 cysteine residues), immunoglobulins, a-2-macroglobulin, fibronectins, vitronectins, fibrinogens, lipases, and the like. Proteins, peptides, enzymes, antibodies and combinations thereof, are general classes of stabilizers contemplated for use in the present invention.
  • a presently preferred protein for use in the formation of a coating is albumin.
  • proteins such as a-2-macroglobulin, a known opsonin, could be used to enhance uptake of the coated (e.g., shell encased) particles of substantially water insoluble pharmacologically active agents by macrophage-like cells, or to enhance the uptake of the coated (e.g., shell encased) particles into the liver and spleen.
  • Specific antibodies may also be utilized to target the nanoparticles to specific locations.
  • synthetic polypeptides containing cysteine residues are also good candidates for formation of a coating (e.g., a shell) about the substantially water insoluble pharmacologically active agents.
  • a coating e.g., a shell
  • These synthetic polypeptides can be chemically unmodified, or, optionally, chemically modified (for example, by the introduction of sulfhydryl and/or disulfide linkages).
  • Exemplary unmodified synthetic polypeptides contemplated for use in the practice of the present invention are such materials as synthetic polyamino acids (optionally containing cysteine residues and/or disulfide groups), polyvinyl alcohol, polyhydroxyethyl methacrylate, polyacrylic acid, polyethyloxazoline, polyacrylamide, polyvinyl pyrrolidinone, polyalkylene glycols, polylactides, polyglycolides, polycaprolactones, or copolymers thereof, and the like, and suitable combinations of any two or more thereof.
  • synthetic polyamino acids optionally containing cysteine residues and/or disulfide groups
  • polyvinyl alcohol polyhydroxyethyl methacrylate
  • polyacrylic acid polyethyloxazoline
  • polyacrylamide polyvinyl pyrrolidinone
  • polyalkylene glycols polylactides, polyglycolides, polycaprolactones, or copolymers thereof, and the like, and suitable combinations
  • unmodified synthetic polypeptides contemplated for use in the practice of the present invention listed above are good candidates for chemical modification (for example, by the introduction of sulfhydryl and/or disulfide linkages) and coating formation (e.g., shell formation, caused, for example, by the crosslinking thereof).
  • contemplated for use in the practice of the present invention are such materials as polyvinyl alcohol modified to contain free sulfhydryl groups and/or disulfide groups; polyhydroxyethyl methacrylate modified to contain free sulfhydryl groups and/or disulfide groups; polyacrylic acid modified to contain free sulfhydryl groups and/or disulfide groups; polyethyloxazoline modified to contain free sulfhydryl groups and/or disulfide groups; polyacrylamide modified to contain free sulfhydryl groups and/or disulfide groups; polyvinyl pyrrolidinone modified to contain free sulfhydryl groups and/or disulfide groups; polyalkylene glycols modified to contain free sulfhydryl groups and or disulfide groups; polylactides, polyglycolides, polycaprolactones, or copolymers thereof, modified to contain free sulfhydryl groups and/or disulfide groups;
  • Biocompatible polymer(s) i.e., the stabilizing agent
  • the stabilizing agent is typically added at a concentration in the range of about 0.001 to about 50 % (w/v), more preferably in the range of about 0.1 % to about 25% (w/v), with a presently preferred range of about 0.5% to about 5% (w/v), as measured in the final mixture prior to evaporation and lyophilization.
  • Suitable solvents utilized in accordance with the present invention include chloroform, methylene chloride, ethyl acetate, ethanol, tetrahydrofuran, dioxane, acetonitrile, acetone, dimethyl sulfoxide, dimethyl formamide, methyl pynolidinone, and the like, as well as mixtures of any two or more thereof.
  • Additional solvents contemplated for use in the practice of the present invention include soybean oil, coconut oil, olive oil, safflower oil, cotton seed oil, sesame oil, orange oil, limonene oil, C1-C20 alcohols (e.g., 1-butanol, 2-butanol, 1-pentanol, 3-methyl 1-butanol, and the like), C2-C20 esters (e.g., butyl acetate, isobutyl acetate, isopropyl acetate, n-isopropyl acetate, and the like), C3-C20 ketones, polyethylene glycols, aliphatic hydrocarbons (e.g., heptane, pentane, and the like), aromatic hydrocarbons, halogenated hydrocarbons, and combinations thereof.
  • C1-C20 alcohols e.g., 1-butanol, 2-butanol, 1-pentanol, 3-methyl 1-butanol, and the like
  • Organic media can also be employed to suspend or dissolve the substantially water insoluble pharmacologically active agent.
  • Organic media contemplated for use in the practice of the present invention include any nonaqueous liquid that is capable of suspending or dissolving the pharmacologically active agent, but does not chemically react with either the polymer employed to produce the shell, or the pharmacologically active agent itself.
  • Examples include vegetable oils (e.g., soybean oil, olive oil, and the like), coconut oil, safflower oil, cotton seed oil, sesame oil, orange oil, limonene oil, aliphatic, cycloaliphatic, or aromatic hydrocarbons having 4-30 carbon atoms (e.g., n-dodecane, n-decane, n-hexane, cyclohexane, toluene, benzene, and the like), aliphatic or aromatic alcohols having 2-30 carbon atoms (e.g., octanol, and the like), aliphatic or aromatic esters having 2-30 carbon atoms (e.g., ethyl caprylate (octanoate), and the like), alkyl, aryl, or cyclic ethers having 2-30 carbon atoms (e.g., diethyl ether, tetrahydrofuran, and the like), alkyl or ary
  • Especially prefened combinations of organic media contemplated for use in the practice of the present invention typically have a boiling point of no greater than about 200°C, and include volatile liquids such as dichloromethane, chloroform, ethyl acetate, benzene, and the like (i.e., solvents that have a high degree of solubility for the pharmacologically active agent, and are soluble in the other organic medium employed), along with a higher molecular weight (less volatile) organic medium.
  • volatile liquids such as dichloromethane, chloroform, ethyl acetate, benzene, and the like
  • solvents that have a high degree of solubility for the pharmacologically active agent, and are soluble in the other organic medium employed
  • these volatile additives help to drive the solubility of the pharmacologically active agent into the organic medium. This is desirable since this step is usually time consuming. Following dissolution, the volatile component may be removed by evaporation (optionally under vacuum).
  • Suitable solvent and/or organic media is typically added at a concentration in the range of about 0.01 % (w/v) to about 50 % (w/v), as measured in the final mixture prior to evaporation and lyophilization.
  • Particles of pharmacologically active agent(s) associated with a coating are delivered as a suspension in a biocompatible aqueous liquid.
  • This liquid may be selected from water, saline, a solution containing appropriate buffers, a solution containing nutritional agents such as amino acids, sugars, proteins, carbohydrates, vitamins or fat, and the like.
  • inventions comprising administering a pharmacologically active agent which must be administered in multiple doses over a cycle time which is less than the cycle time of administration of non-invention formulations of the pharmacologically active agent.
  • invention methods comprise administering the pharmacologically active agent in the invention formulation over a reduced cycle time.
  • cycle time means the time between administration of consecutive single doses of a pharmacologically active agents which must be administered in multiple doses.
  • Reduced cycle times contemplated for use in the present invention can be described in both absolute and percentage terms. Measured in absolute terms, reduced cycle times are cycle times which have been reduced from the cycle time of the conventional mode of administration of the same dose by any number of days from about 1 day to about 18 days. Measured in percentage terms, reduced cycle times are cycle times which have been reduced from the cycle time of the conventional mode of administration of the same dose by about 5% up to about 85% of the cycle time of the conventional mode of administration of the same dose.
  • the invention formulation of this taxane can be administered in multiple doses at a reduced cycle time of 1-20 days as required to provide maximum benefit from treatment.
  • the reduced cycle time afforded by the low toxicity of the invention formulations permits more flexibility in treatment than the higher cycle time (i.e., not less than 3 weeks) necessitated by the higher toxicity of conventional formulations of paclitaxel (i.e., TaxolTM) and docetaxel (i.e., TaxotereTM).
  • inventions for reducing the myelosuppressive effects of a pharmacologically active agent administered to a patient in need thereof.
  • invention methods comprise administering the pharmacologically active agent in the invention formulation.
  • the pharmacologically active agent is a taxane (e.g., paclitaxel, docetaxel, and the like).
  • inventions for reducing the neurotoxicity of a pharmacologically active agent administered to a patient in need thereof.
  • Invention methods comprise administering the pharmacologically active agent in the invention formulation.
  • the pharmacologically active agent is a taxane (e.g., paclitaxel, docetaxel, and the like).
  • inventions comprising administering pharmacologically active agent(s) to a patient having a disease capable of treatment by the pharmacologically active agent(s).
  • invention methods comprise administering suitable pharmacologically agent(s) in the invention formulation to the patient.
  • one aspect of this embodiment comprises administering suitable pharmacologically active agent(s) capable of treating the proliferative disease in the invention formulation to a patient in need thereof.
  • suitable pharmacologically active agent(s) comprise anti-neoplastic agent(s) (for example, taxane (e.g., paclitaxel, docetaxel, and the like), and the like), or the like.
  • a further aspect of this embodiment comprises administering suitable pharmacologically active agent(s) capable of treating the cancer in the invention formulation to a patient having the cancer.
  • Administration can be accomplished in a variety of ways, e.g., intravenously.
  • suitable pharmacologically active agent(s) comprise an anti-neoplastic agent (for example, a taxane (e.g., paclitaxel, docetaxel, and the like), and the like), or the like; and/or the cancer treatable by systemic administration is selected from metastatic breast cancers, malignant melanomas, lung cancers, ovarian cancers, head and neck cancers, prostate cancers, or the like.
  • an anti-neoplastic agent for example, a taxane (e.g., paclitaxel, docetaxel, and the like), and the like
  • the cancer treatable by systemic administration is selected from metastatic breast cancers, malignant melanomas, lung cancers, ovarian cancers, head and neck cancers, prostate cancers, or the like.
  • an additional aspect of this embodiment comprises administering suitable pharmacologically active agent(s) capable of treating the cancer in the invention formulation to a patient having the cancer.
  • Administration can be accomplished in a variety of ways, e.g., intraarterially and/or via injection.
  • suitable pharmacologically active agent(s) comprise an anti-neoplastic agent (for example, a taxane (e.g., paclitaxel, docetaxel, and the like), and the like), or the like; and/or the cancer treatable by localized administration is selected from primary and secondary liver tumors (e.g., hepatocellular carcinoma, multifocal hepatoma, and the like), solid tumors with local-regional involvement (e.g., metastatic breast cancer, prostatic cancer, pancreatic cancer, non-small cell lung cancer (squamous cell cancer), colon cancer, renal cancer, intestinal sarcoma, esophageal cancer, melanoma, ependymoma, head and or neck cancer, and the like), or the like.
  • the dose of suitable pharmacologically active agent administered in accordance with this aspect of the present invention is typically larger than doses administered as part of conventional formulations, and is commonly greater than about 50 mg.
  • an additional aspect of this embodiment comprises administering to the patient the suitable pharmacologically active agent(s) in the invention formulation without the use of any steroid(s).
  • Admimstration can be accomplished in a variety of ways, e.g., intravenously.
  • the suitable pharmacologically active agent(s) whose conventional administration requires corticosteroid premedication comprises a taxane (e.g., paclitaxel, docetaxel, and the like), or the like; and/or the disease is selected from cancers (e.g., metastatic melanomas, renal cell carcinomas, and the like), or the like.
  • biochemotherapy agent(s) can be administered to the patient in combination with the suitable pharmacologically active agent(s) in the invention formulation without the use of any steroid(s).
  • biochemotherapy agent(s) can be administered prior to and/or conc rently with, as well as separately from or jointly with, the suitable pharmacologically active agent(s) in the invention formulation.
  • the biochemotherapy agents are selected from cytokines (e.g., interleukins (e.g., IL-2), interferons, G-CSF, and the like), or the like.
  • an additional aspect of this embodiment comprises administering to the patient the suitable pharmacologically active agent(s) in the invention formulation without the use of any cytokine.
  • a suitable pharmacologically active agent e.g., taxane (for example, paclitaxel, docetaxel, and the like), and the like
  • a suitable pharmacologically active agent e.g., taxane (for example, paclitaxel, docetaxel, and the like)
  • a cumulative dose of greater than about 250 mg/m 2 every 3 weeks a cumulative dose of greater than about 100-130 mg/m 2 every 1 week, and the like.
  • the suitable pharmacologically active agent whose conventional admimstration at a particular dose regimen requires further administration of a cytokine comprises an anti-neoplastic agent (for example, taxane(s) (e.g., paclitaxel, docetaxel, and the like), and the like), or the like; and/or the disease is selected from cancers (e.g., metastatic melanomas, renal cell carcinomas, and the like), or the like.
  • an anti-neoplastic agent for example, taxane(s) (e.g., paclitaxel, docetaxel, and the like), and the like
  • cancers e.g., metastatic melanomas, renal cell carcinomas, and the like
  • inventions comprise administering to the patient a suitable pharmacologically active agent(s) in the invention formulation, wherein the invention formulation has been dispersed within a matrix of suitable biocompatible material. Administration can be accomplished in a variety of ways, e.g., intravenously.
  • the suitable pharmacologically active agent is a taxane (e.g., paclitaxel, docetaxel, and the like); the localized areas are selected from sites of brain tumors, sites of tumors within the peritoneal cavity (e.g., ovarian cancer, metastatic disease, and the like), or the like; and the extended period of time is in a range from about 1 day to about 1 year.
  • a taxane e.g., paclitaxel, docetaxel, and the like
  • the localized areas are selected from sites of brain tumors, sites of tumors within the peritoneal cavity (e.g., ovarian cancer, metastatic disease, and the like), or the like
  • the extended period of time is in a range from about 1 day to about 1 year.
  • temperature sensitive materials e.g., copolymers of polyacrylamides, copolymers of polyalkylene glycols and/or polylactide/glycolides, and the like
  • gels could be made of other polysaccharides (e.g., chemically modified hyaluronic acid, and the like) and/or proteins (e.g., albumin, and the like) for controlled release of drugs from nanoparticle formulations.
  • matrix-dispersed formulations can be delivered locally by a variety of means of local delivery, as discussed above (e.g., implantation directly into the brain or the peritoneal cavity after surgical removal of the brain tumor or peritoneal-located tumor, respectively, and the like).
  • the invention formulations can be injected in a liquid formulation of the temperature sensitive materials which gels at the tumor site and provides for slow release of the pharmacologically active agent(s).
  • the concentration of the invention formulation e.g., CapxolTM, for invention formulations of paclitaxel
  • the crosslink density of the alginate gelled bead e.g., the crosslink density of the alginate gelled bead, and the size of the implanted beads
  • the rate of release of the drug can be controlled.
  • the invention formulations which are dispersed in matrices of the above mentioned biocompatible polymers exhibit a number of potential advantages.
  • these matrix-dispersed formulations can provide a controlled, sustained release formulation of pharmacologically active agent.
  • these matrix-dispersed formulations can provide a locally deliverable formulation of pharmacologically active agent.
  • these matrix-dispersed formulations can result in lower toxicity to the local tissue sunounding the localized treatment site (e.g., the brain tissue, for administration to treat brain tumors) and lower systemic toxicity.
  • the localized treatment site e.g., the brain tissue, for administration to treat brain tumors
  • Other advantages of these matrix-dispersed formulations are readily apparent to those of skill in the art.
  • inventions comprise orally administering an invention formulation of the pharmacologically active agent(s) in combination with an intestinal cell efflux inhibitors).
  • Intestinal cell efflux inhibitors contemplated for use in the present invention include cyclosporin, FK506 (i.e., tacrolimus), compounds which are effective to inhibit the function of the P-glycoprotein efflux pump (associated with intestinal cells) or other like efflux pumps, and the like, and suitable combinations of any two or more thereof.
  • the oral administration of the formulation of the pharmacologically active agent is believed to be facilitated by the oral administration (either in parallel (i.e., concurrent with administration of the agent formulation) or in series (i.e., prior to administration of the agent formulation)) of the intestinal cell efflux inhibitor(s) (e.g., cyclosporin and FK506).
  • the intestinal cell efflux inhibitor(s) is believed to inhibit the function of certain efflux pumps (e.g., the P-glycoprotein efflux pump) on intestinal cell walls. Uninhibited by the intestinal cell efflux inhibitor(s), these efflux pumps would likely promptly pump any absorbed formulation of the pharmacologically active agent (e.g., taxane) out of its associated intestinal cell.
  • the intestinal cell efflux inhibitor(s) is contained within an invention formulation.
  • the invention formulation of the pharmacologically active agent(s) and the invention formulation of intestinal cell efflux inhibitor(s) can be separate formulations (for serial administration), or the same formulation (for parallel administration). Once these formulation(s) have been prepared, they can be administered to a patient suffering from any disease in which treatment with the pharmacologically active agent (for example, taxanes (e.g., paclitaxel, docetaxel, and the like), and the like) shows some benefit, utilizing various dose regimens (e.g., dose amounts, cycle times, and the like) necessary to effect treatment of the disease.
  • the pharmacologically active agent for example, taxanes (e.g., paclitaxel, docetaxel, and the like), and the like
  • various dose regimens e.g., dose amounts, cycle times, and the like
  • inventions comprise administering to the patient 25-75% of the conventionally effective dosage level of each of the suitable pharmacologically active agent(s) in the invention formulation.
  • the reduced dosage levels promote lower toxicities caused by long term use of the suitable pharmacologically active agent(s), while inco ⁇ oration of the suitable pharmacologically active agent(s) in the invention formulation promotes a longer half- life for the concentration of the administered agent(s) in the patient and thereby provides similar treatment effects as the conventional dosage level.
  • the combination of suitable pharmacologically agent(s) comprises a taxane (e.g., paclitaxel, docetaxel, and the like), and a steroid, and about 50% of the conventionally effective dosage level of each of the taxane and the steroid is administered as part of the invention formulation.
  • a taxane e.g., paclitaxel, docetaxel, and the like
  • a steroid e.g., paclitaxel, docetaxel, and the like
  • the amount administered per single dose typically can vary from about 1 mg/m 2 to about 2000 mg/m 2 or greater, and/or the administration time commonly can vary from about 1 minutes to about 30 minutes, and/or the cycle time between consecutive doses generally can be vary from about 1 day to about 20 days.
  • the organic medium within the polymeric shell may be varied, a large variety of pharmacologically active agents may be utilized, and a wide range of proteins as well as other natural and synthetic polymers may be used in the formation of the walls of the polymeric shell. Applications are also fairly wide ranging. Other than biomedical applications such as the delivery of drugs, diagnostic agents (in imaging applications), artificial blood and parenteral nutritional agents, the polymeric shell structures of the invention may be inco ⁇ orated into cosmetic applications such as skin creams or hair care products, in perfumery applications, in pressure sensitive inks, and the like.
  • paclitaxel is dissolved in 3.0 ml methylene chloride.
  • the solution was added to 27.0 ml of human serum albumin solution (1 % w/v).
  • the mixture was homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification was performed at 9000-18,000 psi while recycling the emulsion for at least 5 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system was transferred into a Rotary evaporator, and methylene chloride was rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 20-30 minutes.
  • the resulting dispersion was translucent, and the typical diameter of the resulting paclitaxel particles was 160-220 (Z-average, Malvem Zetasizer).
  • the dispersion was further lyophilized for 48 hrs. without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • the pu ⁇ ose of this example is to demonstrate the formation of nanoparticles of paclitaxel by using cavitation and high shear forces during a sonication process.
  • 20 mg paclitaxel is dissolved in 1.0 ml methylene chloride.
  • the solution is added to 4.0 ml of human serum albumin solution (5% w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I.Q.) in order to form a crude emulsion, and then transfened into a 40 kHz sonicator cell.
  • the sonicator is performed at 60-90% power at 0 degree for 1 min (550 Sonic Dismembrator).
  • the mixture is transfened into a Rotary evaporator, and methylene chloride is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 20-30 minutes.
  • the typical diameter of the resulting paclitaxel particles was 350-420 nm (Z-average, Malvern Zetasizer).
  • the dispersion was further lyophilized for 48 hrs. without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • the following example demonstrates the effect of adding surfactants which are used in the conventional solvent evaporation method.
  • a series of experiments was conducted employing a similar procedure to that described in Example 1, but a surfactant such as Tween 80 (1% to 10%) is added to the organic solvent. It was found that after removal of the methylene chloride, a large number of paclitaxel crystals is obtained having an average size of 1-2 micron, as viewed by light microscopy and under polarized light. The crystals grow within a few hours to form very large needle-like crystals, with a size in the range of about 5-15 micron. A similar phenomenon is observed with other commonly used surfactants, such as Pluronic F-68, Pluronic F 127, Cremophor EL and Brij 58.
  • the conventional solvent evaporation method utilizing conventional surfactants in combination with a protein such as albumin is not suitable for the formation of submicron drug particles (e.g., paclitaxel) without a polymeric core, while using a polar solvent (e.g., methylene chloride).
  • a polar solvent e.g., methylene chloride
  • paclitaxel is dissolved in 0.55 ml chloroform and 0.05 ml ethanol.
  • the solution is added to 29.4 ml of Tween 80 solution (1 % w/v), which is presaturated with 1% chloroform.
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogemzer (Avestin).
  • the emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • Example 5 The resulting system was transfened into a Rotary evaporator, and the chloroform was rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes. The resulting dispersion was opaque, and contained large needle-like crystals of the drug. The initial size of the crystals (observed also by polarized light), was 0.7- 5 micron. Storage of the dispersion for several hours at room temperature led to further increase in crystal size, and ultimately to precipitation.
  • Example 5 Example 5
  • This example describes the process by which sterile-filterable drug particles can be obtained.
  • 30 mg paclitaxel is dissolved in 0.55 ml chloroform and 0.05 ml ethanol.
  • the solution is added to 29.4 ml of human serum albumin solution (1 % w/v), which is presaturated with 1% chloroform.
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogemzer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and the chloroform is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes.
  • the resulting dispersion is translucent, and the typical diameter of the resulting paclitaxel particles is 140-160 nm (Z-average, Malvern Zeta Sizer).
  • the dispersion is filtered through a 0.22 micron filter (Millipore), without any significant change in turbidity, or particle size. HPLC analysis of the paclitaxel content revealed that more than 97% of the paclitaxel was recovered after filtration, thus providing a sterile paclitaxel dispersion.
  • This example describes the process by which sterile-filterable drug particles can be obtained.
  • 225 mg paclitaxel is dissolved in 2.7 ml chloroform and 0.3 ml ethanol.
  • the solution is added to 97 ml of human serum albumin solution (3 % w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and the chloroform is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes.
  • the resulting dispersion is translucent, and the typical diameter of the resulting paclitaxel particles is 140-160 nm (Z-average, Malvern Zeta Sizer).
  • the dispersion is filtered through a 0.22 micron filter (Sartorius, sartobran 300), without any significant change in turbidity, or particle size.
  • HPLC analysis of the paclitaxel content typically revealed that 70-100% of the paclitaxel could be recovered after filtration, depending on the conditions employed. Thus, a sterile paclitaxel dispersion was obtained.
  • the sterile dispersion was aseptically filled into sterile glass vials and lyophilized without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • This example describes the process by which sterile-filterable drug particles can be obtained at higher homogenization pressures than utilized in Example 6.
  • 225 mg paclitaxel is dissolved in 2.7 ml chloroform and 0.3 ml ethanol.
  • the solution is added to 97 ml of human serum albumin solution (3 % w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Microfluidics).
  • the emulsification is performed at 30,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and the chloroform is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes.
  • the resulting dispersion is translucent, and the typical diameter of the resulting paclitaxel particles is 120-160 nm (Z-average, Malvern Zeta Sizer).
  • the dispersion is filtered through a 0.22 micron filter (Sartorius, sartobran 300), without any significant change in turbidity, or particle size.
  • HPLC analysis of the paclitaxel content typically revealed that 70-100% of the paclitaxel could be recovered after filtration, depending on the conditions employed. Thus, a sterile paclitaxel dispersion was obtained.
  • the sterile dispersion was aseptically filled into sterile glass vials and lyophilized without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • isorese ⁇ ine (a model drug) is dissolved in 3.0 ml methylene chloride.
  • the solution is added to 27.0 ml of human serum albumin solution (1 % w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 5 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and methylene chloride is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 20- 30 minutes.
  • the resulting dispersion is translucent, and the typical diameter of the resulting isorese ⁇ ine particles was 120-140 nm (Z-average, Malvern Zetasizer).
  • the dispersion was filtered through a 0.22 micron filter (Millipore).
  • the sterile dispersion was further lyophilized for 48 hrs. without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • Isorese ⁇ ine The effect of ethanol addition on reducing particle size is demonstrated for Isorese ⁇ ine.
  • 30 mg Isorese ⁇ ine is dissolved in 2.7 ml methylene chloride and 0.3 ml ethanol.
  • the solution is added to 27.0 ml of human serum albumin solution (1 % w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification was performed at 9000- 18,000 psi while recycling the emulsion for at least 5 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system was transfened into a Rotary evaporator, and methylene chloride was rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 20-30 minutes.
  • the resulting dispersion was translucent, and the typical diameter of the resulting isorese ⁇ ine particles was 90-110 nm (Z-average, Malvern Zetasizer).
  • the dispersion was filtered through a 0.22 micron filter (Millipore).
  • Example 12 The sterile dispersion was further lyophilized for 48 hrs. without adding any cryoprotectant. The resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline. The particle size after reconstitution was the same as before lyophilization.
  • Example 12 The sterile dispersion was further lyophilized for 48 hrs. without adding any cryoprotectant. The resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline. The particle size after reconstitution was the same as before lyophilization. Example 12
  • paclitaxel is dispersed in 0.6 ml ethanol. At this concentration (50 mg/ml), the paclitaxel is not completely soluble and forms a supersaturated dispersion.
  • the dispersion is added to 29.4 ml of human serum albumin solution (1 % w/v). The mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude dispersion, and then transfened into a high pressure homogenizer (Avestin). The emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and the ethanol is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes.
  • the resulting dispersion particle size is extremely broad, ranging from about 250 nm to several microns.
  • paclitaxel is dispersed in 1.3 ml ethanol. At this concentration (approx. 24.5 mg/ml), the paclitaxel is completely soluble in ethanol.
  • the solution is added to 28.7 ml of human serum albumin solution (1 % w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I. Q.) in order to form a crude dispersion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 6 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size of the dispersion desired.
  • the resulting system is transfened into a Rotary evaporator, and the ethanol is rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 15-30 minutes.
  • the resulting dispersion particle size was extremely broad, ranging from about 250 nm to several microns. Observation under the microscope revealed the presence of large particles and typical needle shaped crystals of paclitaxel. These particles were too large for intravenous injection.
  • Example 14 in addition to Example 12 above, demonstrates that the use in the invention process of solvents such as ethanol that are freely miscible in water results in the formation of large particles with very broad particle size distribution and as such cannot be used alone for the invention process.
  • the invention process specifically excludes the use of water miscible solvents when used alone for the dissolution or dispersion of the drug component.
  • the invention process requires that such solvents, when used, be mixed with essentially water immiscible solvents to enable formation of invention nanoparticles.
  • Paclitaxel raw material is usually present as needle shaped crystals of varying sizes typically between 5-500 microns.
  • the presence of crystals in a drug formulation for intravenous injection is obviously detrimental if crystals are present in size above a few microns due to potential blockage of capillaries.
  • the solubility of drug crystals in general would be lower than for amo ⁇ hous drug, thereby lowering the bioavailability of the drug following intravenous administration. It is also known that as the loading of the drug in a formulation is increased, the tendency for crystallization also increases. Thus it is advantageous that the formulation contain the drug in essentially amo ⁇ hous form.
  • X-ray powder diffraction was used to determine the crystalline or non-crystalline nature of paclitaxel in the lyophilized powder formulation.
  • the following samples were analyzed: Sample 1 - Paclitaxel powder; Sample 2 - Lyophilized serum albumin; Sample 3 - a physical mixture of paclitaxel and albumin; and Sample 4 - formulated paclitaxel.
  • Each sample was x-rayed from 2° to 70° 2Q angles using CuKa radiation, an accelerating voltage of 40KeV/30mA, a step size of 0.05° 2Q and a data acquisition time of 2.0 seconds per step.
  • Sample 1 showed strong peaks typical of a crystalline sample. The most intense paclitaxel peak was located at 5.1° 2Q.
  • Sample 2 showed broad humps typical of amo ⁇ hous material.
  • Sample 3 showed largely the broad humps of Sample 2, but in addition, the peak at 5.1° 2Q of paclitaxel was visible.
  • Sample 4 the formulated paclitaxel showed no evidence of crystalliniry characteristic of paclitaxel and appeared identical to Sample 2, indicating the presence of substantially amo ⁇ hous pharmacologically active agent in the formulated sample.
  • the amo ⁇ hous nature of the nanoparticles produced according to the invention stands in direct contrast to the products produced by other methods described in the art for producing nanoparticles.
  • the use of grinding techniques as described in U.S. Patent 5,145,684 (Liversidge et al), and as described by Liversidge-Merisko et al, Pharmaceutical Research 13(2):272-278 (1996), produces a substantially crystalline product.
  • Nanoparticles of paclitaxel (the active ingredient of TaxolTM) were prepared as described above in Example 1. This formulation of the drug was tested in a MX-1 human mammary tumor xenograft model in mice. The mice were implanted subcutaneously with the MX-1 mammary tumor and the treatment was initiated when the tumor reached approximately 150 - 300 mg in size. This occuned by day 12 and the treatment was initiated on day 13 after initial seeding.
  • Tumor bearing mice were treated with paclitaxel nanoparticles at a dose of 20 mg/kg, given by bolus intravenous injection as a suspension in saline for five consecutive days.
  • the treated group included five animals.
  • the control tumor bearing group of five animals received only saline on the same schedule.
  • the size of the tumors was monitored as a function of time.
  • the control group showed a tremendous increase in tumor weight.
  • All the animals in this group were sacrificed between day 28 and day 39.
  • the treatment group on the other hand showed remarkable efficacy as all animals had no measurable tumors by day 25.
  • the animals in this group were all sacrificed on day 39, at which time they showed no evidence of recurrence and no evidence of tumor.
  • the results are shown in Figure 1.
  • Collagen induced arthritis model in the Louvain rat was used to test the therapeutic effect of paclitaxel nanoparticles on arthritis.
  • the paw sizes of the experimental animals were monitored to evaluate the seriousness of arthritis. After the arthritis was fully developed (usually -9-10 days after collagen injection), the experimental animals were divided into different groups to receive either paclitaxel nanoparticles lmg/kg q.o.d, or paclitaxel nanoparticles 0.5mg/kg + prednisone 0.2mg/kg q.o.d. (combination treatment) intraperitoneally for 6 doses, then one dose per week for three weeks. The paw sizes were measured at the beginning of treatment (day 0) and every time the drug was injected.
  • the paclitaxel-containing nanoparticles demonstrated therapeutic effect on arthritis. To avoid side effects of long term use of both paclitaxel and the steroid, it is probably better to choose a combination treatment to get similar effect but only half the dosage of each drug.
  • targeting moieties such as proteins, antibodies, enzymes, peptides, oligonucleotides, sugars, polysaccharides, and the like
  • This targeting ability can be utilized for therapeutic or diagnostic pmposes.
  • the materials used for the preparation of intravenous delivery systems may be polymeric (e.g., polyethylene, polyvinyl, polypropylene tubing, and the like), or glass.
  • Standard medical grade tubing is known to contain hydrophobic moieties on the inner surfaces thereof. These moieties are thus available to come in contact with the injection solution. Indeed, such tubing is specifically tailored, as are the catheters, to present hydrophobic moieties in contact with the treatment solution so as to reduce the abso ⁇ tion of aqueous material to the tubing. However, any hydrophobic moieties in the treatment solution will likely bind to both the catheter tubing and other components of the delivery system.
  • hydrophobic pharmacologically active agent can become sequestered in the inner walls ofthe tubing catheter and delivery vessel. Consequently, the dosing of hydrophobic pharmacologically active agents can be enatic, since a substantial portion ofthe active agent can become absorbed to the walls ofthe tubing.
  • hydrophobic pharmacologically active agent is used to treat a disease, a significant reduction in the effective dose of active agent can lead to a therapeutic failure. The failure is particularly striking when employing therapeutic moieties which require that the active agent be present above a certain level, yet the therapeutic window is narrow.
  • a novel method for the intravenous introduction of a hydrophobic pharmacologically active agent has now been developed.
  • a biocompatible coating e.g., albumin
  • the propensity ofthe active agent to become attached to the tubing is dramatically reduced.
  • the invention method comprises placing a protective coating of a biocompatible polymer (e.g., albumin) around the hydrophobic drug and placing the resulting composition in a hydrophobic polymeric delivery system.
  • the invention methods are therefore capable of improving the delivery of a variety of hydrophobic therapeutics.
  • Intravenous administration of therapeutics predisposes the therapeutic to at least one pass through the liver.
  • therapeutics for example, drugs, imaging agents, and the like
  • that therapeutic is filtered through the liver, a significant portion of that therapeutic is taken up and sequestered by the liver, and therefore, not available for systemic distribution.
  • a protein such as albumin
  • liver sequestration upon intravenous administration is alleviated.
  • Albumin for example, is known to pass through the liver and become generally distributed throughout the patient.
  • the sequestration of albumin by the liver does not occur to the same degree as toxic compounds or drugs which have hepatic receptors (or other mechanisms) which initiate processes which result in their removal from the blood stream.
  • a biocompatible polymer e.g., a human albumin coating
  • the drug then bypasses the liver and is generally distributed through all organ systems.
  • a novel method for bypassing the liver which comprises encapsulating a drug in a human liver albumin (essentially a physiological component). In this way, more ofthe drug becomes available for systemic therapy.
  • a decrease in the production of metabolic byproducts of hepatocellular drug degradation In addition to the increased availability ofthe drug, there is a decrease in the production of metabolic byproducts of hepatocellular drug degradation.
  • Both the increase in liver bypass and decrease in byproducts of drug metabolism provide a synergistic improvement in the overall drug efficacy. This improved efficacy extends to all drugs and materials that are encapsulated in human albumin.
  • Paclitaxel the active ingredient of TaxolTM
  • cremaphor/ethanol i.e., TaxolTM
  • paclitaxel produces myelosuppressive effects that limit the repeat administration ofthe drug and preclude retreatment of a patient for at least 3 weeks in order to allow blood counts ofthe patient to return to normal It was postulated that due to the non-toxic compatible nature ofthe drug carrier ofthe present invention, viz. human albumin, the toxic side effect of myelosuppression may be greatly reduced.
  • Sprague dawley rats were given paclitaxel in commercial formulation (available from Bristol Myers Squibb (BMS) in cremaphor/ethanol) or prepared by the invention method as nanoparticles with albumin. Both formulations were administered by tail vein injection. A single dose level of 5 mg/kg was administered for the BMS formulation, whereas two dose levels of 5 mg/kg and 12 mg/kg were administered for the invention formulation (Capxol). The white blood cell counts ofthe rats were monitored daily after administration as an index of myelosuppression.
  • BMS Bristol Myers Squibb
  • the BMS formulation is administered as an intravenous infusion lasting anywhere from 1 hour to 24 hours.
  • formulations according to the present invention due to the use of a non-toxic carrier, can be administered to a patient readily as an intravenous bolus (i.e., in a period less than 1 hour) without the toxicity problems seen in the BMS formulation that is used clinically today.
  • the effective dose of paclitaxel for a patient typically lies between 200-500 mg, depending on the patient body weight or body surface.
  • the BMS formulation has to be administered at a final dosing concentration of 0.6 mg/ml, requiring large infusion volumes (typically in the range of about 300-1000 ml).
  • invention formulations e.g., Capxol
  • invention formulations do not have these limitations and can be administered at a desired concentration. This enables clinicians to treat patients by a rapid intravenous bolus that can be administered in as little as a few minutes.
  • the invention formulation is reconstituted to a dosing concentration of 20 mg/ml, the infusion volume for a total dose of 200-500 mg is only 10-25 ml, respectively. This is a great advantage in clinical practice.
  • the nanoparticle formulation according to the invention showed no apparent toxic effects.
  • the nanoparticle formulation at a dose of 103 mg/kg showed some reduction in body weight ofthe mice, but even this high dose was not lethal.
  • Doses of approximately 1000 mg/kg, 800 mg/kg and 550 mg/kg were all lethal but differing in time to lethality, which ranged between a few hours to 24 hours.
  • the lethal dose ofthe invention formulation is greater than 103 mg/kg but less than 550 mg/kg.
  • the lethal dose ofthe invention formulation of paclitaxel is substantially higher than that ofthe commercial BMS formulation. This has great significance in clinical practice where higher doses of chemotherapeutic drugs may be administered for more effective oncolytic activity with greatly reduced toxicity.
  • cyclosporine is dissolved in 3.0 ml methylene chloride.
  • the solution is then added into 27.0 ml ofhuman serum albumin solution (1% w/v).
  • the mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer model: Tempest I.Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin).
  • the emulsification was performed at 9000-18,000 psi while recycling the emulsion for at least 5 cycles.
  • the number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size ofthe dispersion desired.
  • the resulting system was transfened into a Rotavap and methylene chloride was rapidly removed at 40°C, at reduced pressure (30 mm Hg), for 20-30 minutes.
  • the resulting dispersion was translucent and the typical diameter ofthe resulting cyclosporine particles was 160-220 (Z-average, Malvern Zetasizer).
  • the dispersion was further lyophilized for 48 hours, without adding any cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • the particle size after reconstitution was the same as before lyophilization.
  • cyclosporine is dissolved in 3.0 ml of a suitable oil (sesame oil containing 10% orange oil). The solution is then added into 27.0 ml ofhuman serum albumin solution (1% v/w). The mixture is homogenized for 5 minutes at low RPM (Vitris homogenizer, model: Tempest I.Q.) in order to form a crude emulsion, and then transfened into a high pressure homogenizer (Avestin). The emulsification is performed at 9000-18,000 psi while recycling the emulsion for at least 5 cycles. The number of cycles through the homogenizer can vary from about one up to hundreds of cycles, depending on the size ofthe dispersion desired. The resulting dispersion had a typical diameter of 160-220 (Z-average, Malvern Zetasizer).
  • the dispersion could be used directly or lyophilized for 48 hours by optionally adding a suitable cryoprotectant.
  • the resulting cake could be easily reconstituted to the original dispersion by addition of sterile water or saline.
  • Nanoparticles of cyclosporine (Capsorine IN.) prepared as described above (Examples 22 and 23) were reconstituted in saline and administered to a first group of 3 Sprague Dawley rats by intravenous bolus.
  • a second group of 3 rats were given Sandimmune IN., which contains Cremaphor/Ethanol after dilution in saline. Each group received the same dose of 2.5 mg/kg. Blood samples were taken at times 0, 5, 15, 30 (minutes) 1, 2, 4, 8, 24, 36 and 48 (hours). Levels of cyclosporine in the blood were assayed by HPLC and typical PK parameters were determined.
  • the PK curves showed typical decay over time as follows:
  • Nanodroplets of cyclosporine prepared above were administered in orange juice, to a first group of 3 Sprague Dawley rats by oral gavage.
  • a second group of 3 rats were given Neoral, a commercially available microemulsion formulation containing emulsifiers, after dilution in orange juice, also by oral gavage.
  • Each group received the same dose of 12 mg/kg in an identical volume of orange juice.
  • Blood samples were taken at times 0, 5, 15, 30 (minutes) 1, 2, 4, 8, 24, 36 and 48 (hours).
  • Levels of cyclosporine in the blood were assayed by HPLC and typical PK parameters were determined. The PK curves showed typical decay over time as follows:
  • nanodroplet formulation (Capsorine Oral) ofthe present invention shows a similar PK behavior to the commercially available formulation (Neoral).
  • an invention formulation of paclitaxel (CapxolTM) was administered by intravenous injection to 17 human subjects exhibiting advanced metastasis (Ofthe 17 subjects enrolled in the Phase I clinical study, 6 subjects had malignant melanoma and the remaining 11 subjects had metastatic breast cancer).
  • the initial dose level (paclitaxel single dose) admimstered to each subject was 135 mg/m 2 , and the subsequent doses were escalated to the next higher paclitaxel single dose level (per conventional paclitaxel dose regimen) if there were no significant adverse effects in the subject.
  • the maximum single dose admimstered was 375 mg/m 2 paclitaxel, with this dose being given in saline over a period of 30 minutes or less. This maximum single dose is significantly higher than the approved dose of paclitaxel (175 mg/m 2 ) administered as part ofthe conventional formulation, TaxolTM.
  • TaxolTM One ofthe dose limiting toxicities in the administration of paclitaxel as part of the conventional formulation, TaxolTM, is the neurotoxicity (neuropathy) of TaxolTM, which is seen in up to 70% of patients receiving TaxolTM.
  • an invention formulation of paclitaxel shows significantly reduced incidence of neurotoxicity (neuropathy) even when utilizing higher paclitaxel dose levels than are permitted with TaxolTM.
  • TaxolTM Another significant dose limiting toxicity for TaxolTM is myelosuppression, which is seen in up to 90% of patients receiving TaxolTM at a dose of 175 mg/m 2 (i.e., subject's neutrophil count drops to ⁇ 2000/mm 3 ).
  • WBC white blood cell
  • CapxolTM invention formulations of paclitaxel
  • CapxolTM Two ofthe malignant melanoma subjects being treated with the invention formulations of paclitaxel (CapxolTM) did not exhibit any progression ofthe malignant melanoma for periods ranging from more than 15 weeks to more than 30 weeks. It is su ⁇ rising that admimstration ofthe invention formulations of paclitaxel (CapxolTM) generates a response in these patients because paclitaxel, admimstered in the conventional formulation of TaxolTM, previously had not been found to generate a significant response in the treatment of malignant melanoma.
  • AUC area under the curve
  • paclitaxel administered as CapxolTM
  • CapxolTM can have a positive effect in the treatment of psoriasis.
  • paclitaxel administered as CapxolTM
  • other proliferative diseases e.g., multiple sclerosis, vascular restinosis, and the like.
  • an invention formulation of paclitaxel was administered intra-arterially to more than 25 patients exhibiting primary and/or secondary liver tumors and/or solid tumors with local-regional involvement.
  • the clinical study protocol called for administration ofthe invention formulation of paclitaxel (CapxolTM) by intra-arterial injection (chemo-embolization) into the artery feeding the tumor that was to be treated.
  • the hepatic artery could be catheterized and the drug injected directly into the artery feeding the tumor.
  • the treated patients included patients with hepatocellular carcinoma, multifocal hepatoma, metastatic breast cancer, prostatic cancer, pancreatic cancer, non-small cell lung cancer (squamous cell cancer), colon cancer, renal cancer, intestinal sarcoma, esophageal cancer, melanoma, ependymoma, and head & neck cancer.
  • responses to treatment e.g., reductions in cancer size, and the like
  • were observed for most of these patients including patients with lung cancer, prostatic cancer, breast cancer, and/or hepatic disease.
  • intra-arterial injection was remarkably well tolerated by the treated patients, as toxicity in these patients was very limited. Late toxicity included slight alopecia, a slight reduction in WBC and a sense of fatigue lasting 4-5 days.
  • intra-arterial injection ofthe invention formulation (Capxol) of paclitaxel allowed the administration ofthe drug substance paclitaxel in a manner not previously possible. Previously only a very limited dose of paclitaxel could be administered intra-arterially due to the low solubility of paclitaxel.
  • the invention formulation allows intra-arterial admimstration at much higher doses thereby allowing a more efficacious treatment of the disease. Furthermore, this mode of treatment results in responses to cancers e.g., prostatic and pancreatic cancer which are normally considered nonresponsive.
  • paclitaxel administered in its conventional, approved formulation, TaxolTM
  • IL-2 has not previously been evaluated for treatment of metastatic melanoma because ofthe requirement for corticosteroid premedication prior to administration of TaxolTM.
  • Corticosteroid premedication is required to prevent anaphylactic-like hypersensitivity reactions associated with the cremophor vehicle of TaxolTM.
  • the corticosteroids introduced due to this requirement cause lysis of LAK cells, resulting in the loss of any benefit from administration of IL-2.
  • TaxolTM cannot be used in combination with drugs like IL-2 or interferon.
  • the invention formulations of paclitaxel are cremophor-free formulations of paclitaxel which are freely dispersible in aqueous media. Because the invention formulations are cremophor-free, the risk of hypersensitivity reactions on administration ofthe invention formulations is remote. Thus, corticosteroid premedication is not required with administration of invention formulations of paclitaxel (e.g., CapxolTM).
  • This discovery permits treatment of patients in need thereof with a combination of paclitaxel (CapxolTM) and IL-2 (e.g., metastatic melanoma patients who have failed conventional chemotherapy, patients with renal cell carcinoma, and the like).
  • paclitaxel e.g., CapxolTM
  • IL-2 paclitaxel
  • An invention formulation of paclitaxel is administered intravenously over approximately 30 minutes at an initial dose of 200 mg/m 2 on day 1 (before start of IL-2 dosing) and at a dose of 100 mg/m 2 on day 6 (upon completion of IL-2 dosing).
  • IL-2 is administered intravenously at 18 MIU/m 2 over 6 hours beginning after administration ofthe initial dose of paclitaxel, followed by 18 MIU/m 2 administered intravenously over 12 hours, followed by 18 MIU/m 2 administered intravenously over 24 hours, followed by 4.5 MIU/m 2 /day administered intravenously over 3 consecutive days, with the total administration time of IL-2 being 120 hours from day 1 to day 6.
  • Other suitable schedules for drug administration reported in the literature can also be utilized.
  • patients with renal cell carcinoma can be treated with a combination of paclitaxel (e.g., CapxolTM) and IL-2.
  • paclitaxel e.g., CapxolTM
  • IL-2 is used for the treatment of these patients.
  • paclitaxel administered in its approved formulation, TaxolTM
  • TaxolTM cannot be administered to these patients, alone or in combination with IL-2, because ofthe need for corticosteroid premedication prior to administration of TaxolTM, as discussed above.
  • invention formulations of paclitaxel e.g., CapxolTM
  • any situation requiring treatment of patients utilizing IL-2, interferon or other cytokine biochemotherapy agents is also suitable for the treatment of these patients utilizing invention formulations of taxanes (e.g., paclitaxel, docetaxel, and like taxanes which require steroid premedication) in combination therapy with the cytokine biochemotherapy.
  • taxanes e.g., paclitaxel, docetaxel, and like taxanes which require steroid premedication
  • taxanes e.g., paclitaxel (in its approved formulation TaxolTM), docetaxel (in its approved formulation TaxotereTM), and other taxanes which require steroid premedication
  • biochemotherapy agents e.g., interleukins, interferons, other cytokines, and the like
  • use of invention formulation of taxanes obviates the need for steroid administration and makes possible the treatment of patients with combination chemotherapy. This combination chemotherapy can provide added benefits to the treated patient.
  • Hypersensitivity reactions are a problem associated with administration of paclitaxel (in its commercially available formulation, TaxolTM), docetaxel (in its commercially available formulation, TaxotereTM), and other taxanes.
  • TaxolTM the commercially available formulation ofthe anti-cancer agent paclitaxel, is available as a drug which can be dissolved prior to administration utilizing cremophor (polyethoxylated castor oil) as a surfactant and ethanol as a vehicle.
  • cremophor polyethoxylated castor oil
  • ethanol a surfactant
  • Hypersensitivity reactions e.g., due to drug- and/or vehicle-induced toxicity
  • TaxolTM occur in about 40% of all patients receiving TaxolTM. Because of this documented hypersensitivity reaction, all patients to whom TaxolTM is to be administered are required to take premedication that comprises corticosteroids and antihistamines prior to receiving TaxolTM.
  • TaxotereTM the commercially available formulation ofthe anticancer agent docetaxel, is another taxane that is dissolved in the surfactant Tween-80. Because of analogous hypersensitivity reactions, administration of TaxotereTM also requires steroid premedication.
  • the invention formulations of taxanes e.g., CapxolTM (which comprises nanoparticles of paclitaxel stabilized by human serum albumin), and the like) were found su ⁇ risingly to not induce any hypersensitivity reactions in human patients.
  • taxanes e.g., paclitaxel (in CapxolTM formulation), docetaxel, and the like
  • paclitaxel in CapxolTM formulation
  • docetaxel and the like
  • myelosuppression is a problem (i.e., a dose limiting toxicity) associated with administration of paclitaxel in its commercially available formulation, TaxolTM.
  • a large number of patients receiving TaxolTM are required to take G-CSF support to prevent excessive myelosuppression.
  • the neutrophil counts or absolute granulocyte counts may drop to dangerously low levels (i.e., less than about 500/mm 3 ).
  • patients receiving invention formulations of taxanes do not require cytokine (e.g., G-CSF, and the like) support or any other means of support required to avoid excessive myelosuppression.
  • cytokine e.g., G-CSF, and the like
  • Delivering pharmacologically active agents e.g., antiinfectives, immunosuppressives, chemotherapeutic agents, and the like
  • pharmacologically active agents e.g., antiinfectives, immunosuppressives, chemotherapeutic agents, and the like
  • a specific treatment site e.g., localized tumor site, and the like
  • the biocompatible materials discussed above can be employed in several physical forms
  • these biocompatible materials provide matrices from which pharmacologically active agent(s) (e.g., taxanes (paclitaxel, docetaxel, and the like), and the like) can be locally released and/or dispersed (e.g., at the site ofthe solid tumor (e.g., brain (brain cancer), peritoneum (ovarian cancer), and the like), and the like) by diffusion across and/or degradation ofthe matrix.
  • pharmacologically active agent(s) e.g., taxanes (paclitaxel, docetaxel, and the like), and the like
  • dispersed e.g., at the site ofthe solid tumor (e.g., brain (brain cancer), peritoneum (ovarian cancer), and the like), and the like) by diffusion across and/or degradation ofthe matrix.
  • the invention formulations of paclitaxel can be dispersed within a matrix ofthe biocompatible material(s) discussed above.
  • This matrix provides a sustained release formulation of paclitaxel for the treatment of brain tumors and tumors within the peritoneal cavity (e.g., ovarian cancer, metastatic diseases, and the like).
  • the invention formulations of paclitaxel e.g., CapxolTM
  • the invention formulations of paclitaxel can be injected in a liquid formulation ofthe temperature sensitive materials which gels at the tumor site and provides for slow release of CapxolTM.
  • CapxolTM (an invention formulation of paclitaxel) was reconstituted in a 1 % solution of sodium alginate. The solution was then pumped through a device to make gel beads ofthe CapxolTM/alginate solution in aqueous calcium chloride.
  • Some beads containing CapxolTM were separated from the solution and placed in culture media. The release of paclitaxel from the beads containing CapxolTM into the media was measured over time at 37°C. It was found that sustained release of paclitaxel could be obtained from the beads containing CapxolTM for periods of up to 28 days.
  • CapxolTM Additional beads containing CapxolTM were separated from the solution and implanted into the peritoneal cavity of rats to determine release of paclitaxel from within the beads. The rats were sacrificed 2 weeks later and the gelled beads retrieved. Measurement of paclitaxel within the beads showed that most ofthe drug had been released into the peritoneal cavity ofthe rats.
  • paclitaxel or other pharmacologically active agents, e.g., docetaxel (commercially available as TaxotereTM), and the like
  • cycle time i.e., the time between consecutive administrations of doses ofthe pharmacologically active agent
  • dose levels i.e., the amount of each dose of pharmacologically active agent
  • invention formulations ofthe taxane can be administered with cycle times in the range of from about 1 day to about 3 weeks or more, with a preferable cycle time of about 3 to about 15 days, as required to provide maximum benefit from treatment to the patient in need thereof.
  • doses of 25-250 mg/m 2 of paclitaxel in invention formulations can be administered every 1-20 days in patients suffering from any disease in which treatment with paclitaxel shows some benefit.
  • TaxolTM the typical dose level is 100-135 mg m 2 with a cycle time of 1 week.
  • invention formulations ofthe taxane e.g., paclitaxel, docetaxel, and the like
  • typical dose levels e.g., doses greater than 135 mg/m 2 and up to 400 mg/m 2 or greater, as required to treat patients suffering from any disease in which treatment with a taxane shows some benefit.
  • Taxanes e.g., paclitaxel, docetaxel, and the like
  • invention formulations of a taxane can be administered orally in combination with oral cyclosporin and or oral FK506 (i.e., tacrolimus).
  • Invention formulation(s) ofthe taxane and/or cyclosporin and/or FK506 can be prepared in accordance with the invention methods. These formulation(s) can be prepared as separate formulations (for oral administration in series), or as a combination formulation comprising the taxane and at least one of cyclosporin and FK506 (for oral administration in parallel).
  • these formulation(s) can be administered to a patient suffering from any disease in which treatment with a taxane shows some benefit, utilizing various dose amounts and cycle times necessary to effect treatment ofthe disease.
  • the oral administration ofthe taxane formulation is believed to be facilitated by the oral administration (either in parallel (i.e., concunent with administration ofthe taxane formulation) or in series (i.e., prior to administration ofthe taxane formulation)) of cyclosporin and/or FK506.
  • Cyclosporin and/or FK506 are believed to inhibit the function of P-glycoprotein efflux pumps on intestinal cells. Uninhibited, these P-glycoprotein efflux pumps would likely pump any absorbed paclitaxel formulation out of its associated intestinal cell.
  • An exemplary invention composition referred to herein as ABI-007, is prepared in dosage forms containing 30 mg, lOOmg or 300mg of paclitaxel in a vial.
  • ABI-007 When reconstituted with 0.9% Sodium Chloride Injection, USP, ABI-007 forms a colloidal suspension of paclitaxel stabilized with Human Albumin, USP.
  • the formulation contains no other added excipients. The sterility ofthe product is assured by an aseptic manufacturing process and sterile filtration.
  • ABI-007 The two major components of ABI-007 are paclitaxel and Human Albumin, USP. Since human albumin is soluble in Sodium Chloride Injection, USP, ABI-007 can be reconstituted to the desired concentration of paclitaxel, which is limited only by the solubility limits for human albumin.
  • Admimstration of ABI-007 to the patient can be accomplished by intravenous infusion at a dosage directed in the climcal protocol.
  • the total mg of paclitaxel to be administered should be calculated by the physician, using the height/weight conversion chart for body surface area of the patient.
  • the reconstituted sample should be milky and homogeneous without visible particulates. If particulates are visible, the vial should be mildly agitated again to ensure complete re-suspension, prior to use;
  • Administer the reconstituted ABI-007 solution by IV infusion over a desired period e.g., bolus injection, 30 min, 1 hr, 2hr, 3 hr, 6hr, 24hr, 48 hr, 72hr or 96 hour infusions.
  • a desired period e.g., bolus injection, 30 min, 1 hr, 2hr, 3 hr, 6hr, 24hr, 48 hr, 72hr or 96 hour infusions.
  • Prefened administration period is no greater than about 3 hours. Do not use in-line filters, as they are not necessary.
  • compositions and dosage forms in polymeric or glass containers were diluted with sterile normal saline to concentrations of 0.5, 1, 5, 10, and 15 mg/mland stored at room temperature and under refrigerated conditions.
  • the compositions were found to be homogeneous and stable for at least 24 hours to three days under these conditions. Particle size measurements performed at several time points indicated no change in size distribution. No precipitation was seen under these conditions.
  • the diluted compositions were stable in the presence of different polymeric materials such as teflon, silastic, polyethylene, tygon, and other standard infusion tubing materials.
  • the compositions were stable under frozen, refrigerated as well as room temperature conditions.
  • Stability studies ofthe non-diluted invention compositons show stability under refrigerated and room temperature conditions for over 1 year. Freezing, refrigeration or room temperature storage does not adversely affect the product.
  • compositions are diluted with saline or dextrose or other aqueous medium, the drug becomes available or is released in solution.
  • dissolution media may be utilized for example, media containing surfactants such as tweens, proteins such as serum albumin, etc. to measure release and availability ofthe drug. Dissolution and release testing are performed by techniques well described in the art.
  • Dosage forms of invention compositions are prepared in unit vessels of glass, metallic, organic, inorganic or polymeric origin or combinations thereof with suitable closures that are glass, metallic, organic, inorganic or polymeric in origin or combinations thereof.
  • the unit vessels may contain between 1 mg to 1000 mg of active drug.
  • Suitable dosage forms include but are not limited to 5mg, lOmg, 20mg, 30mg, 50mg, 80mg, lOOmg, 150mg, 200mg, 250mg, 300mg, 350mg, 400mg, 450mg, 500mg, 600mg, 800mg, lOOOmg. These dosage forms are stable when stored frozen, refrigerated or at room temperature and for select periods at elevated temperatures.
  • Paclitaxel is a novel anti-microtubule agent that promotes the assembly of microtubules from tubulin dimers and stabilizes microtubules by preventing depolymerization. The stability results in the inhibition ofthe normal dynamic reorganization ofthe microtubule network that is essential for the vital inte ⁇ hase and mitotic cellular function.
  • paclitaxel induces abnormal arrays or "bundles" of microtubules throughout the cell cycle and multiple anays of microtubules during mitosis. It has recently been reported that paclitaxel facilitates apoptosis in cells containing the Bcl-2 gene. It is postulated that paclitaxel phosphorylates the Bcl-2 gene and induces programmed cell death.
  • Several tumors are known to be Bcl-2 positive (prostate tumors).
  • a pilot study has been conducted to determine the efficacy ofthe ABI-007 formulation of paclitaxel.
  • Female athymic NCr-nu mice were implanted with MX-1 human mammary tumor fragments and treated with daily (five days) injections of ABI-007, ABI-007 vehicle and TAXOL.
  • the efficacy of ABI-007 was demonstrated with 100% survival ofthe animals in the high dose group (45 mg/kg/day) of both formulations of ABI-007. In contrast, no animals survived after receiving vehicle, and only 40% of animals survived on the highest dose of TAXOL (30 mg/kg/day).
  • ABI-007 The antitumor activity of ABI-007 was also demonstrated in an in vitro study.
  • Murine leukemia cells L1210) were cultured in suspension and dilutions of ABI-007 or TAXOL (0.01 to 0.5 ⁇ g/mL) were added to the cell cultures in Falcon 96- well plates. Cells were counted after 48 hours.
  • Both ABI-007 and TAXOL exhibited antitumor activity in L1210 murine leukemia cells.
  • the IC 50 for ABI-007 and TAXOL were 0.014 ⁇ g/mL and 0.010 ⁇ g/mL respectively.
  • the slightly lower IC 50 for TAXOL is probably caused by the toxic effect ofthe Cremophor/ethanol vehicle.
  • 3 H- ABI-007 and 3 H-TAXOL exhibited a similar pattern of paclitaxel metabolism into highly polar metabolite(s) as measured by blood HPLC profile; however, metabolism appeared significantly slower for 3 H-ABI-007 with 44.2% of blood radioactivity remaining as paclitaxel at 24 hours post-dose versus 22.4% for 3 H- TAXOL.
  • the excretion of radioactivity occuned only minimally in the urine and predominantly in the feces for 3 H- ABI-007, which is similar to the reported excretion patterns for 3 H- TAXOL.
  • Tissue radioactivity levels are higher following 3 H-ABI-007 admimstration than 3 H- TAXOL admimstration for 9 of 14 tissues.
  • 3 H- ABI-007 was present in significantly greater quantities than 3 H-TAXOL in prostate, spleen, and pancreas and to a lesser extent in bone, kidney, lung, heart and muscle.
  • the tissue/blood concentration ratios were higher in all tissues for the 3 H-ABI-007 dosed animals as a result ofthe lower blood levels. This supports the rapid distribution of 3 H- ABI-007 from the blood to the tissues suggested by the blood kinetic data.
  • 3 H-paclitaxel formulated in ABI-007 showed a similar pharmacokinetic profile to 3 H- paclitaxel formulated in TAXOL, but tissue/blood concentration ratios and metabolism rates differ significantly. A significantly lower level of total radioactivity was detected for ABI-007-treated animals than for TAXOL-treated animals in the 2-minute post admimstration blood sample, indicating that the 3 H- ABI-007 is more rapidly distributed out ofthe blood.
  • the area under the curve was proportionally increased.
  • the level of parent compound after 24 hours was increased by a factor of 8.5 (0.04 ppm - 0.34 ppm), going from the 9 mg/kg dose to the 148 mg/kg dose.
  • 3 H- ABI-007 was well-tolerated as a single IV bolus at all four doses tested.
  • the plot ofthe AUC for total radioactivity versus IV dose admimstered shows a linear, but disproportionate response with a slope greater than one. This response was also observed when comparing 5 and 10 mg/kg doses of 3 H-TAXOL tested in previous studies.
  • HPLC analysis of 24-hour post-dose blood samples showed a significant percentage (7-19%) of blood activity as unchanged paclitaxel, indicating that paclitaxel in ABI-007 is slowly metabolized in vivo and that total exposure to paclitaxel is significant for ABI-007-treated animals.
  • Paclitaxel is not a new drug substance. Preclinical studies of ABI-007 have therefore been limited to specific studies which evaluate the toxicity ofthe new formulation and the potential use of higher doses of paclitaxel in humans than is currently approved.
  • a pilot study was conducted to compare the LD 50 of ABI-007, TAXOL and their carrier vehicles following a single bolus intravenous administration into mice.
  • the LD 50 and LD 10 for ABI-007 were 447.4 and 371.5 mg/kg of paclitaxel, 7.53 and 5.13 mg/kg of paclitaxel in TAXOL, and 1325 and 794 mg/kg ofthe TAXOL vehicle (corresponds to a dose of 15.06 and 9.06 mg/kg TAXOL), respectively.
  • the LD 50 for ABI-007 was 59 times greater than TAXOL and 29 times greater than the TAXOL vehicle alone. Most deaths in the TAXOL and TAXOL vehicle treated groups occuned on day 0 which suggests the toxicity of TAXOL is due to an acute reaction to the vehicle. Such acute reactions are unlikely to occur in human patients treated with ABI-007 because ofthe difference in vehicles.
  • a pilot study was conducted to compare the LD 50 of ABI-007 and TAXOL following daily (five days) intravenous administrations into mice. The results indicated that ABI-007 is less toxic than TAXOL.
  • the LD 50 of ABI-007 was 76.2 mg/kg of paclitaxel, compared to 8.07 mg/kg of paclitaxel in TAXOL.
  • the LD 50 for ABI-007 was 9.4 times higher than for TAXOL. All deaths (11) for TAXOL-treated animals occurred on days 0 - 4 after start of treatment while deaths for ABI-007-treated animals (5) occurred on days 6 or 9 after the start of treatment.
  • ABI-007 is less toxic than TAXOL when admimstered in multiple doses at daily intervals.
  • Human patients will initially receive ABI-007 infusion over a period of approximately 30 minutes with 3-week intervals between treatments, rather than a 1 day interval as in the animal studies. Toxicity from treatment with ABI-007 is considerably less than treatment with TAXOL.
  • TAXOL ® and one treated with ABI-007 died on day 4. In comparison, all but one animal treated with ABI-007 at doses up to and including 120 mg/kg survived. One animal treated with 90 mg/kg ABI-007 had an unexplained death.
  • mice were observed for overt manifestations of toxicity immediately following dosing, 1 hour and 4 hours post-administration and once daily for 7 or 30 days. All animals in each group appeared normal immediately post-administration. At 1 and 4 hours post-administration, rats in the TAXOL-treated groups were observed to have piloerection and staggering gaits.
  • ABI-007 produced significantly less (P ⁇ 0.05) myelosuppression than TAXOL as determined by white cell counts at days 1 and 7 and a highly significant (PO.01) reduction in white cell counts at 10.
  • the recovery period from the white blood cell suppression after a 5 mg/kg dose of paclitaxel was only about 7 days for ABI-007 but was about 14 days for TAXOL.
  • ABI-007 also showed significantly less decreases in weight at days 1 and 10 than TAXOL.
  • the TAXOL vehicle (Cremophor/ethanol) had a very strong neutropenic effect.
  • the TAXOL vehicle decreased WBCs for days 1 and 3 (PO.01) when compared to the ABI-007 vehicle and also significantly decreased WBCs on day 1 when compared to ABI-007 (P ⁇ 0.05). Significant decreases in body weights (P ⁇ 0.05) were also observed for the TAXOL vehicle when compared to both ABI-007 and its vehicle.
  • TAXOL myelosuppression from TAXOL.
  • ABI-007 vehicle human albumin
  • neutropenia from treatment in human patients with ABI-007 should be considerably less than treatment than with TAXOL.
  • the animals in all three treatment groups exhibited a common syndrome of gastrointestinal symptoms, edema, vasculitis, and organ and tissue abnormalities. These toxic effects, indicate that the animals were reacting to human albumin, a constituent ofthe formulation common to all three groups. That ABI-007-treated animals had more severe and numerous clinical symptoms suggests that the cytotoxic effects of paclitaxel in the ABI-007 exacerbated the effects ofthe human albumin.
  • ABI-007 In a Phase I, open-label, dose-ranging trial of ABI-007 therapy in patients with advanced solid tumors conducted at M.D. Anderson Cancer Center, more than 80 courses of ABI-007 were administered intravenously (IV) over 30 minutes, every 3 weeks to 19 patients.
  • the total number of doses of ABI-007 administered was 83.
  • the maximum dose administered was 375 mg/m 2 , which was administered to six patients (between 25-85 minutes).
  • the maximally tolerated dose was determined to be 300 mg/m 2 , which was also administered to six patients (between 27-60 minutes).
  • the Phase I study has been closed for enrollment and the maximum tolerated dose (MTD) for ABI-007 has been established to be 300 mg/m 2 .
  • MTD maximum tolerated dose
  • Patient Diagnoses and Condition The primary diagnoses for the 19 patients emolled were as follows: Six (31.6%) patients had melanoma, 12 (63.2%) had breast cancer and 1 (5.3%) had a primary diagnosis of unknown origin. Histology showed 11 (57.9%) patients diagnosed with invasive ductal carcinoma; 6 (31.6%) patients had malignant melanoma; 1 (5.3%) patient diagnosed with invasive lobular carcinoma, and 1 (5.3%) patient with Paget's disease ofthe nipple. At baseline all 19 patients reported having a Zubrod performance status score of 2 or less. Eleven
  • Prior Therapy All 19 patients had received prior therapy. Nineteen (100%) patients had prior chemotherapy; 6 (31.6%) patients had prior hormonotherapy; 7 (36.8%) patients had prior immunotherapy; 17 (89.5%) patients had prior radiotherapy; and 17 (89.5%) patients had prior surgery.
  • Dose Escalation Four dose levels were utilized in this study. The starting dose level of ABI-007 was 135 mg/m 2 . Four patients were treated at this dose. The dose was escalated by 50% to 200 mg/m 2 , at which 3 patients were treated. The dose was further escalated by 50% to 300 mg/m 2 at which 6 patients were treated. The final escalation in dose was by 25% to 375 mg/m 2 at which 6 patients were treated. It was eventually determined that 300 mg/m 2 was the maximum tolerated dose (MTD). At dose levels of 135mg/m 2 and less than 200 mg/m 2 , there were no adverse events of myelosuppression or peripheral neuropathy.
  • MTD maximum tolerated dose
  • ABI-007 was well tolerated at doses up to 300 mg/m 2 , which were delivered over a period of 27-60 minutes. Most adverse events were Grade 1 or 2 (93%), required no action (83%) and were resolved (78%). No deaths occurred during the study. Five patients had reductions in their study drug dosages, changes in their frequency, interruptions in dosing and/or discontinuation of study drug during the study. All five patients had these changes to their study drug regimen as the result of the development of unacceptable toxicities. Of these five patients, four reported sensory changes, three reported vision-related toxicities, two experienced asthenia, and two patients experienced thrombocytopenia. All other patients receiving a dose from 135 mg/m 2 to 300 mg/m 2 showed no such evidence of thrombocytopenia.
  • Grade 3 Toxicities There were 27 occurrences of Grade 3 reported toxicities, including: fatigue and sensory changes, both at 5 (19.2%), stomatitis 3 (11.5%), dianhea and keratitis, both at 2 (7.7%), bone pain, constipation, cough, dyspnea, hypoxia, infection, nausea, pleural effusion, skin reaction, and vomiting all were reported as single occurrences (3.8%). (% of total is reflective ofthe number of occurrences out ofthe total 27 Grade 3 reported toxicities.)
  • Hematologic Toxicities There was a total of 13 (68.4%) patients that reported an Absolute Neutrophil Count (ANC) ⁇ 2000 cells/mm 3 . Ofthe total 13 patients, 2 (15%) were at a dose level of 135 mg/m 2 , 1 (8%) patient was at dose level 200 mg/m 2 , 5 (38%) patients were at dose level 300 mg/m 2 , and 5 (38%) patients with ANC ⁇ 2000 cells/mm 3 were at the dose level of 375 mg/m 2 .
  • ANC Absolute Neutrophil Count
  • Sensory Toxicities Sensory changes (including sensory loss, tingling, paresthesia, and peripheral neuropathy) were reported by 12 (63.16%) out of 19 patients. There were a total of 23 reported occunences of sensory toxicities. Eight (34.8%) occunences of sensory toxicity were reported as Grade 1 (including two patients with pre-existing condition at baseline). A total of 10 (43.5%) occurrences of sensory toxicity were rated as Grade 2 and 5 (21.7%) reported cases were rated as Grade 3. Two out ofthe 23 (8.7%) occunences were pre-existing sensory toxicities experienced by two patients prior to study drug administration. These two patients went on to receive study drug and there were no reports of any sensory toxicities while receiving ABI-007.
  • the third patient reported a Grade 3 sensory toxicity while at a dosing level of 301.07 mg/m 2 .
  • Patient continued on the next cycle at a dose reduced level of 198.93 mg/m 2 .
  • the fourth patient (dosing level 301.50 mg/m 2 ) experienced a Grade 2 sensory toxicity.
  • Study dose level was reduced to 200 mg/m 2 for the following cycle.
  • Ocular or Vision Toxicities A total of 17 occurrences of visual disturbances, including decreased vision associated with burning sensation, foreign body sensation and photophobia were reported by 10 (52.6%) patients while receiving ABI-007. In the opinion ofthe Investigator, 10 (63%) ofthe 17 visual toxicities were possibly study drug related.
  • the first patient experienced three separate visual disturbances: blurred vision (Grade 2), keratitis (Grade 3) and another occunence of blurred vision (Grade 1), all at dose level 375.28 mg/m 2 .
  • Patient's study drug regimen was interrupted; however, the patient continued on the following cycle at a dose reduced level of 198.86 mg/m 2 .
  • the second patient requiring action experienced one occurrence of Grade 2 abnormal vision and a Grade 3 keratitis. Dosing regimen of 370.83 mg/m 2 was interrupted; however, patient resumed dosing at a reduced level of 300 mg/m 2 .
  • the third patient had a Grade 2 occurrence of keratitis while receiving ABI-007 at a dosing level of 301.50 mg/m 2 .
  • Action taken was a dose reduction on the following cycle to 200 mg/m 2 .
  • Amblyopia 3 ( 0.8%) 2 (10.5%)
  • Diarrhea 15 ( 4.2%) 7 (36.8%)
  • Leukopenia 1 ( 0.3%) 1 : 5.3%)
  • the MTD for ABI-007 was established at 300 mg/m 2 .
  • Preliminary, unaudited data from the Phase I trial of ABI-007 (given at 135-375 mg/m 2 /30 min) indicate a lower incidence of adverse events than seen with the published information on TAXOL (given at 175 mg/m 2 /3hr) and TAXOTERE (given at 100 mg/m 2 /l hr).
  • ABI-007 indicates 68% of patients reporting neutropenia at ⁇ 2000 cells/mm 3 , compared to TAXOL (90%) and TAXOTERE indicating 98.5%. ABI-007 indicates 32% of patients (4 out of 6 patients at dose level 375 mg/m 2 ) experiencing neutropenia at ⁇ 500 cells/mm 3 compared to TAXOL (28%) and TAXOTERE (85.9%).
  • Phase I of this trial 19 patients with solid tumor/breast cancer were assigned to four cohorts of at least 3 patients each.
  • the starting ABI-007 dose at 135 mg/m 2 was administered to the first cohort via intravenous infusion at 3 -week intervals. After a minimum of 3 patients in the first cohort had been treated with the starting dose infused over 180 minutes, the infusion duration was decreased to 30 minutes.
  • the ABI-007 doses for the three remaining cohorts were 200, 300, and 375 mg/m 2 , respectively, with each ABI-007 dose infused over a duration of approximately 30 minutes .
  • the paclitaxel AUC mf value increased exponentially versus ABI-007 dose. This dose-dependent pharmacokinetic behavior was reflected by decreasing CL values as the ABI-007 dose was raised from 135 mg/m 2 to 375 mg/m 2 . Similar findings have been reported Taxol.
  • ABI-007 pharmacokinetic parameters obtained in the present study versus published pharmacokinetic parameters for Taxol, both products produced similar paclitaxel AUC ⁇ nf , half-life, and plasma clearance values at similar doses.
  • ABI-007 could be infused intravenously over a shorter duration, i.e., 30 to 45 minutes compared to the intravenous infusion of Taxol, which normally takes 3 to 24 hours.
  • Protocol DM97- 123 is an open non-randomized Phase I/II trial of ABI-007 in patients with solid tumor/breast cancer.
  • Phase I of this protocol 19 patients were entered and were assigned to four cohorts of at least 3 patients each.
  • the starting ABI-007 dose at 135 mg/m 2 was admimstered to the first cohort via intravenous infusion at 3-week intervals. After a minimum of 3 patients in the first cohort had been treated with the starting dose infused over 180 minutes, the infusion duration was decreased to 30 minutes.
  • the ABI-007 doses for the three remaining cohorts were 200, 300, and 375 mg m 2 , respectively, with each ABI-007 dose infused over a duration of approximately 30 minutes.
  • This preliminary clinical pharmacokinetic report summarizes the results of the pharmacokinetic analysis ofthe patients who contributed analyzable plasma drug concentration profiles.
  • Each cohort had a minimum of 3 patients.
  • Each patient received ABI-007 via intravenous infusion at 3 -week intervals.
  • the ABI-007 infusion duration varied from 27 minutes to 180 minutes depending on the targeted ABI-007 dose.
  • Whole blood/plasma samples were collected from each patient at baseline and at 0.25, 0.5, 1, 1.5, 2, 4, 6, 8, 12, 14, 24 and 48 hours from the start ofthe first intravenous infusion of ABI-007.
  • the whole blood/plasma samples were frozen and shipped on dry ice to Alta Analytical Laboratories, El Dorado Hills, California for assay of paclitaxel concentrations.
  • a highly specific and sensitive LC/MS/MS assay was used in the analysis of whole blood/plasma samples. The limit of quantification ofthe assay was 5 ng/mL.
  • Plasma samples were obtained from all four patients assigned to receive the starting ABI-007 dose at 135 mg/m 2 and from the first patient in the second cohort assigned to receive ABI-007 dose at 200 mg/m 2 .
  • Whole blood samples were collected from the remaining patients.
  • Nineteen patients (16 females and 3 males) were enrolled. There were 18 Caucasians (95%) and 1 Hispanic (5%). The median age was 51 years (range: 34 - 83 years). Sixteen ofthe 19 enrolled patients contributed analyzable plasma drug concentration profiles.
  • Pharmacokinetic parameters were determined from each patient's whole blood/plasma paclitaxel concentration profile.
  • the non-compartmental routine in WinNonlin was used in the analysis.
  • the peak or maximum paclitaxel concentration (C max ) and the corresponding peak time (t ⁇ J were observed values.
  • the elimination constant ( ⁇ z ) was obtained by log-linear regression analysis ofthe terminal phase of the whole blood/plasma concentration versus time profile.
  • the elimination half-life (T 1/2 ) was determined by taking the ratio of natural log of 2 and ⁇ z .
  • the area under the curve from time zero to time infinity was obtained by summation of AUC last (area under the curve from time zero to last measurable concentration, calculated by the linear trapezoidal rule) and AUC ext (extrapolated area, estimated by taking the ratio between the last measurable concentration and ⁇ z ).
  • the dose-area relationship i.e., total ABI-007 dose divided by AUC ⁇ nf
  • CL total body clearance
  • V z volume of distribution
  • Descriptive statistics (mean, median, standard deviation, coefficient of variation, maximum and minimum) were computed for pertinent pharmacokinetic parameters by ABI-007 dose. No inferential statistics were performed due to the small sample size per cohort. However, linear regression analysis of AUC lnf versus dose was performed to gain an appreciation of pharmacokinetic linearity, if evident, for the dose range evaluated in this trial.
  • Non-compartmental analysis of individual profiles yielded pertinent pharmacokinetic parameters for each patient.
  • a listing ofthe individual pharmacokinetic parameter values (including C ⁇ , T ⁇ ,, T 1/2 , AUC ⁇ nf , CL and V z ) was also provided by patient number.
  • a summary of pharmacokinetic parameters by targeted ABI-007 dose is presented in the Table above.
  • ABI-007 a Cremophor-free formulation of paclitaxel, offers the advantage of short intravenous infusion duration (e.g., 30 to 40 minutes) while achieving similar AUC ⁇ nf values as TAXOL.
  • Example 48
  • ABI-007 is an effective pharmaceutical for treating patients with higher doses of paclitaxel than used previously and with less severe toxic effects.
  • phase I intra-arterial admimstration, open-label, dose-ranging trial of ABI-007 therapy in patients with advanced loco-regional solid tumors conducted at Insituto Nazionale Tumori, more than 200 courses of ABI-007 were admimstered intra-arterially over 30 minutes every 4 weeks to over 90 patients.
  • the phase I study, open-label, in patients with loco-regional solid tumors is still ongoing. One hundred patients are planned.
  • the primary diagnosis ofthe 74 patients enrolled were as follows: 28 had hepatic-carcinoma (7 primary tumor, 21 secondary tumor) the remaining 46 had tumor in different districts: anal canal (12), bladder (6), head (16), neck (5), lung (2), pancreas (2), uterus (1), vulvae (1) e supernal (1). Mean and median values concerning weight, height and age were reported and divided for type of tumors. Patients with liver carcinoma were treated with single doses between 150-450 mg for a maximum of three cycles. Patients with solid tumors received dose between 150-510 mg for a maximum of 4 cycles.
  • the event intensity was mild in the 71.0%, moderate in the 26.5% and severe in the 2.3% ofthe cases; the 78% ofthe events do not require assistance and all the events recovered without sequelae in 67.4% ofthe cases.
  • the drug conelation was ofthe 77.7% (probable in the 39.5% and possible in the 38.2 ofthe cases).
  • the response is defined according to WHO criteria, partial in 3 cases (11%), stable disease in 13 (46%) and progression in 12 (43%) patients with hepatic carcinoma (28).
  • Patients with head and neck carcinoma (20) showed a complete response in 2 cases (9.5%), partial in 14 cases (66.7%), stable in 2 cases (9.5%) and progression in 2 cases (9.5%).
  • Patients with anal canal carcinoma (12) showed a complete response in 1 case (8.3%), partial in 4 cases (33.4%), stable in 6 cases (50%) and progression in 1 case (8.3%).
  • the remaining 6 patients showed partial response in 1 case (uterus), stable in 2 cases (lung, pancreas) and progression in the last 3 cases (lung, pancreas and suprarenal).
  • invention compositions e.g., those containing drugs such as taxanes
  • Devices contemplated for use with invention compositions include any type of tubing including polymeric tubings that may be utilized to admimster the invention compositions or in general to admimster drugs such as the taxanes or other antiproliferative drugs.
  • Tubings of interest for use in the invention include catheter of any type, intravenous lines, arterial lines, intra- thecal lines, intracranial lines, catheters or tubing that may be guided by suitable means to any location within the subject, e.g., to the site of a stenotic blood vessel such as coronary artery or other artery or vein.
  • Such tubings may also have the capability to carry baloons or stents that are useful for treatment of local narrowing, stenosis, restenosis, plaques including atherosclerotic plaques, thrombotic lesions, sites of hyperplasia, aneurysms or weakness in blood vessels.
  • Stents are also contemplated for use in combination with invention compositions.
  • Stents may be fabricated from organic or inorganic materials, polymeric materials or metals.
  • Invention compositions contemplate the combination ofthe invention pharmacological agents and devices mentioned herein.
  • Combination devices such as those comprising tubings along with baloons, stents, devices for local injection (e.g., into the lumen, into the vessel wall, into the intima ofthe blood vessel, into the endothelial or sub-endothelial layer, into the smooth muscle layer of blood vessels) etc. are also contemplated in combination with invention compositions of pharmacological agents.
  • compositions of pharmacological agents or in general drugs such as the taxanes or other antiproliferative drugs and any drug or drugs contemplated by the invention may be delivered by the devices described above either by flowing through the device, being impregnated or embedded or stored within or with the device, or being able to be released or delivered at a local site of interest by the device or delivered by the device to be systemically available in the subject (e.g., intravenous administration).

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Abstract

La présente invention concerne des compositions et des procédés convenant pour l'administration in vivo d'agents pharmacologiquement actifs essentiellement insolubles dans l'eau (tels que le médicament anticancéreux paclitaxel) dans lesquels un agent actif au plan pharmacologique est administré sous forme de particules en suspension enrobées d'une protéine (laquelle agit comme agent stabilisateur). En particulier, la protéine et l'agent pharmacologiquement actif présents dans un support dispersant biocompatible sont soumis à un cisaillement élevé en l'absence de tout tensioactif classique et en l'absence de matériau noyau polymère pour les particules. Le procédé selon l'invention permet d'obtenir des particules d'un diamètre inférieur au micron. L'utilisation d'une composition spécifique dans des conditions de préparation précises (adjonction d'un solvant polaire à la phase organique), et le choix raisonné de la phase organique appropriée et de la fraction de phase permet la production reproductible de nanoparticules exceptionnellement petites d'un diamètre inférieure à 200 nm qui peuvent être stérilisées par filtration. Le système de particules obtenu dans le cadre de l'invention peut être transformé en une poudre sèche redispersible comprenant des nanoparticules d'un médicament enrobé d'une protéine, insoluble dans l'eau, et d'une protéine libre à laquelle des molécules de l'agent pharmacologique sont liées. On obtient ainsi un système d'administration unique en son genre dans lequel un agent actif au plan pharmacologique est facilement biodisponible (sous forme de molécules liées à la protéine), et où une partie de l'agent est présente dans les particules sans aucune matrice polymère.
PCT/US2000/013954 1999-05-21 2000-05-19 Agents a stabilisation proteinique actifs pharmacologiquement; procedes de fabrication et methodes d'utilisation WO2000071079A2 (fr)

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