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WO2000061638A3 - Detection de cancer et de grossesse anormale au moyen d'anticorps monoclonaux specifiques pour des isoformes de la hcg - Google Patents

Detection de cancer et de grossesse anormale au moyen d'anticorps monoclonaux specifiques pour des isoformes de la hcg Download PDF

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Publication number
WO2000061638A3
WO2000061638A3 PCT/US2000/009776 US0009776W WO0061638A3 WO 2000061638 A3 WO2000061638 A3 WO 2000061638A3 US 0009776 W US0009776 W US 0009776W WO 0061638 A3 WO0061638 A3 WO 0061638A3
Authority
WO
WIPO (PCT)
Prior art keywords
hcgn
hcg
nicked
choriocarcinoma
isoforms
Prior art date
Application number
PCT/US2000/009776
Other languages
English (en)
Other versions
WO2000061638A2 (fr
Inventor
Alexander Krichevsky
Steven Birken
John O'connor
Original Assignee
Univ Columbia
Alexander Krichevsky
Steven Birken
Connor John O
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Columbia, Alexander Krichevsky, Steven Birken, Connor John O filed Critical Univ Columbia
Priority to AU42349/00A priority Critical patent/AU4234900A/en
Priority to CA002369923A priority patent/CA2369923A1/fr
Publication of WO2000061638A2 publication Critical patent/WO2000061638A2/fr
Publication of WO2000061638A3 publication Critical patent/WO2000061638A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La gonadotrophine chorionique humaine (hCG) existe dans le sang et l'urine en tant que variété d'isoformes. L'une d'elles, qui contient des clivages de liaisons peptidiques à l'intérieur de sa sous unité bêta, est désignée hCG coupée (hCGn). Cette isoforme de la hCGn est plus courante dans l'urine des patients présentant certaines malignités et éventuellement dans d'autres maladies de grossesse. L'invention porte sur deux anticorps monoclonaux à une isoforme de la hCGn isolée chez un patient choriocarcinome. Deux analyses immunométriques sur site ont été élaborées à l'aide de ces anticorps, désignés B151 et B152. Le premier présente une bonne spécificité pour hCGn indépendante de la source de la hCGn, forme évacuée par des patients choriocarcinomes, ou la forme de la hCGn provenant de grossesses normales. L'autre anticorps, B152, est exceptionnellement sensible aux fractions glucidiques de hCG choriocarcinome, coupée ou non, étant donné que sa reconnaissance de ligand est fonction des différences glucidiques plutôt que des différences de liaisons peptidiques. Ces deux analyses immunométriques apportent de nouveaux outils de diagnostic fondés sur le mesurage direct de ces isoformes de la hCG.
PCT/US2000/009776 1999-04-12 2000-04-12 Detection de cancer et de grossesse anormale au moyen d'anticorps monoclonaux specifiques pour des isoformes de la hcg WO2000061638A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU42349/00A AU4234900A (en) 1999-04-12 2000-04-12 Detection of cancer and abnormal pregnancy using monoclonal antibodies specific for hcg isoforms
CA002369923A CA2369923A1 (fr) 1999-04-12 2000-04-12 Detection de cancer et de grossesse anormale au moyen d'anticorps monoclonaux specifiques pour des isoformes de la hcg

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12884599P 1999-04-12 1999-04-12
US60/128,845 1999-04-12

Publications (2)

Publication Number Publication Date
WO2000061638A2 WO2000061638A2 (fr) 2000-10-19
WO2000061638A3 true WO2000061638A3 (fr) 2001-04-19

Family

ID=22437271

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/009776 WO2000061638A2 (fr) 1999-04-12 2000-04-12 Detection de cancer et de grossesse anormale au moyen d'anticorps monoclonaux specifiques pour des isoformes de la hcg

Country Status (3)

Country Link
AU (1) AU4234900A (fr)
CA (1) CA2369923A1 (fr)
WO (1) WO2000061638A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003012445A1 (fr) 2001-07-30 2003-02-13 Quest Diagnostics Investments Incorporated Procedes et trousses de detection des ita dans un echantillon biologique
WO2005095458A1 (fr) * 2004-04-02 2005-10-13 The Talwar Research Foundation Anticorps monoclonal chimerique destine a se lier selectivement a hcg avec une haute affinite

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998010282A1 (fr) * 1996-09-06 1998-03-12 Yale University Depistage prenatal du syndrome de down a l'aide de gonadotrophine hyperglycosylee
WO1999041584A2 (fr) * 1998-02-03 1999-08-19 The Trustees Of Columbia University In The City Of New York METHODES DE PREVISION DE L'ISSUE D'UNE GROSSESSE CHEZ UN SUJET PAR DOSAGE BIOLOGIQUE DE hCG

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998010282A1 (fr) * 1996-09-06 1998-03-12 Yale University Depistage prenatal du syndrome de down a l'aide de gonadotrophine hyperglycosylee
WO1999041584A2 (fr) * 1998-02-03 1999-08-19 The Trustees Of Columbia University In The City Of New York METHODES DE PREVISION DE L'ISSUE D'UNE GROSSESSE CHEZ UN SUJET PAR DOSAGE BIOLOGIQUE DE hCG

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
A. KRICHEVSKY ET AL.: "Development, characterization, and application of monoclonal antibodies to the native and synthetic betaCOOH-terminal portion of human chorionic gonadotropin (hCG) that distinguish between the native and desialylated forms of hCG.", ENDOCRINOLOGY, vol. 134, no. 3, March 1994 (1994-03-01), Philadelphia, PA, USA, pages 1139 - 1145, XP000939398 *
G. KOVALEVSKAYA ET AL.: "Early pregnancy human chorionic gonadotropin (hCG) isoforms measured by an immunometric assay for choriocarcinoma-like hCG.", THE JOURNAL OF ENDOCRINOLOGY, vol. 161, no. 1, April 1999 (1999-04-01), Bristol, GB, pages 99 - 106, XP000939395 *
G. KOVALEVSKAYA ET AL.: "Evaluation of nicked human chorionic gonadotropin content in clinical specimens by a specific immunometric assay.", CLINICAL CHEMISTRY, vol. 45, no. 1, January 1999 (1999-01-01), Washington, DC, USA, pages 68 - 77, XP000946000 *
J. BIDART ET AL.: "Monoclonal antibodies to the free beta-subunit of human chorionic gonadotropin define three distinct antigenic domains and distinguish between intact and nicked molecules.", ENDOCRINOLOGY, vol. 131, no. 4, October 1992 (1992-10-01), Philadelphia, PA, USA, pages 1832 - 1840, XP000939365 *
J. O'CONNOR ET AL.: "Recent advances in the chemistry and immunochemistry of human chorionic gonadotropin: impact on clinical measurement.", ENDOCRINE REVIEWS, vol. 15, no. 5, 1994, Baltimore, MD, USA, pages 650 - 683, XP000939376 *
L. COLE ET AL.: "Hyperglycosylated hCG, a potential alternative to hCG in Down syndrome screening.", PRENATAL DIAGNOSIS, vol. 18, no. 9, September 1998 (1998-09-01), Chichester, GB, pages 926 - 933, XP000939392 *
P. BERGER ET AL.: "Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.", THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, vol. 77, no. 2, August 1993 (1993-08-01), Philadelphia, PA, USA, pages 347 - 351, XP000939386 *
S. BIRKEN ET AL.: "Development and characterization of antibodies to a nicked and hyperglycosylated form of hCG from a choriocarcinoma patient.", ENDOCRINE, vol. 10, no. 2, April 1999 (1999-04-01), Totowa, NJ, USA, pages 137 - 144, XP000939394 *

Also Published As

Publication number Publication date
AU4234900A (en) 2000-11-14
WO2000061638A2 (fr) 2000-10-19
CA2369923A1 (fr) 2000-10-19

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