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WO2000061634A3 - Modulateurs variants de l'ecotine de genie genetique agissant sur des serines proteases - Google Patents

Modulateurs variants de l'ecotine de genie genetique agissant sur des serines proteases Download PDF

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Publication number
WO2000061634A3
WO2000061634A3 PCT/US2000/009790 US0009790W WO0061634A3 WO 2000061634 A3 WO2000061634 A3 WO 2000061634A3 US 0009790 W US0009790 W US 0009790W WO 0061634 A3 WO0061634 A3 WO 0061634A3
Authority
WO
WIPO (PCT)
Prior art keywords
ecotin
serine protease
serine
modulators
variant
Prior art date
Application number
PCT/US2000/009790
Other languages
English (en)
Other versions
WO2000061634A2 (fr
Inventor
Charles S Craik
Robert J Fletterick
Original Assignee
Univ California
Charles S Craik
Robert J Fletterick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Charles S Craik, Robert J Fletterick filed Critical Univ California
Priority to AU46433/00A priority Critical patent/AU4643300A/en
Publication of WO2000061634A2 publication Critical patent/WO2000061634A2/fr
Publication of WO2000061634A3 publication Critical patent/WO2000061634A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des protéines de liaison de classe qui se lient spécifiquement à des polypeptides pourvus d'un pli de chymotrypsine (par exemple des sérines protéases). Lorsque les polypeptides cibles sont des sérines protéases, les protéines de liaison peuvent inhiber ou activer la sérine protéase. Les protéines de liaison sont basées sur la structure de l'écotine. Une modification de la terminaison amino ou carboxyle et/ou la randomisation d'au moins une boucle 50s, 60s, 80s ou 100s produiront une banque de variantes de l'écotine à partir de laquelle on peut sélectionner des molécules de liaison (par exemple des modulateurs de protéase) propres à presque toutes les sérines protéases. Selon la variante de l'écotine et la sérine protéase cible, le modulateur peut agir comme inhibiteur ou activateur de la sérine protéase. L'invention traite en outre d'agonistes spécifiques du Facteur IXa.
PCT/US2000/009790 1999-04-12 2000-04-12 Modulateurs variants de l'ecotine de genie genetique agissant sur des serines proteases WO2000061634A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46433/00A AU4643300A (en) 1999-04-12 2000-04-12 Engineering ecotin-variant modulators of serine proteases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29051399A 1999-04-12 1999-04-12
US09/290,513 1999-04-12

Publications (2)

Publication Number Publication Date
WO2000061634A2 WO2000061634A2 (fr) 2000-10-19
WO2000061634A3 true WO2000061634A3 (fr) 2001-03-08

Family

ID=23116349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/009790 WO2000061634A2 (fr) 1999-04-12 2000-04-12 Modulateurs variants de l'ecotine de genie genetique agissant sur des serines proteases

Country Status (2)

Country Link
AU (1) AU4643300A (fr)
WO (1) WO2000061634A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5255841B2 (ja) * 2004-10-15 2013-08-07 ジェネンコー・インターナショナル・インク 競合示差スクリーニング
WO2016054452A1 (fr) * 2014-10-01 2016-04-07 Ansun Biopharma, Inc. Variants d'écotine
CN106226428B (zh) * 2016-07-20 2019-01-01 未名生物医药有限公司 一种快速检测cho细胞发酵液中重组人神经生长因子含量的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5719041A (en) * 1993-09-14 1998-02-17 Genentech, Inc. DNA encoding ecotin homologs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5719041A (en) * 1993-09-14 1998-02-17 Genentech, Inc. DNA encoding ecotin homologs

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
SEONG E.A.: "Mutational analysis for the role of C-terminal region of ecotin, a dimeric inhibitor of pancreatic serine proteases", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 42, no. 4, July 1997 (1997-07-01), pages 799 - 807, XP000952778 *
WANG CHENG-I ET AL: "Isolation of a High Affinity Inhibitor of Urokinase-type Plasminogen Activator by Phage Display of Ecotin", JOURNAL OF BIOLOGICAL CHEMISTRY,THE AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, INC.,,US, vol. 270, no. 20, 1995, pages 12250 - 12256, XP002146128, ISSN: 0021-9258 *
YANG E.A.: "Ecotin: a serine protease inhibitor with two distinct and interacting binding sites", JOURNAL MOLECULAR BIOLOGY, vol. 279, 19 June 1998 (1998-06-19), pages 945 - 957, XP002150104 *
YANG E.A.: "Engineering bidentate macromolecular inhibitors for trypsin and urokinase-type plasminogen activator", JOURNAL MOLECULAR BIOLOGY, vol. 279, 19 June 1998 (1998-06-19), pages 1001 - 1011, XP002150105 *

Also Published As

Publication number Publication date
AU4643300A (en) 2000-11-14
WO2000061634A2 (fr) 2000-10-19

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