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WO2000050575A3 - Sequences d'acides nucleiques de 9-hydroperoxyde lyase d'acides gras - Google Patents

Sequences d'acides nucleiques de 9-hydroperoxyde lyase d'acides gras Download PDF

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Publication number
WO2000050575A3
WO2000050575A3 PCT/US2000/005311 US0005311W WO0050575A3 WO 2000050575 A3 WO2000050575 A3 WO 2000050575A3 US 0005311 W US0005311 W US 0005311W WO 0050575 A3 WO0050575 A3 WO 0050575A3
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plant
lyase
hpo lyase
hpo
cucumber
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PCT/US2000/005311
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English (en)
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WO2000050575A2 (fr
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Kenji Matsui
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Calgene Llc
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Publication of WO2000050575A2 publication Critical patent/WO2000050575A2/fr
Publication of WO2000050575A3 publication Critical patent/WO2000050575A3/fr

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne la 9-hydroperoxyde lyase ou des enzymes de HPO lyase; des produits de recombinaison d'ADN utiles pour l'expression d'une HPO lyase de plante dans une cellule; des produits de recombinaison d'ADN utiles pour l'expression antisens d'une 9-HPO lyase dans une cellule de plante. Ces produits de recombinaison contiennent une séquence d'ADN codant pour la 9-HPO lyase sous le contrôle d'éléments régulateurs capables de diriger de façon préférentielle l'expression de cette 9-HPO lyase dans le tissu de la plante, quand ce produit de recombinaison est exprimé dans une plante transgénique. L'invention concerne également des procédés de mise en application d'une séquence d'ADN codant pour une 9-HPO lyase afin de modifier les aldéhydes volatils dans le tissu de la plante, ainsi que des procédés servant à augmenter la résistance d'une plante à des maladies. Les séquences de 9-HPO lyase illustrées ici sont obtenues à partir d'Arabidopsis.
PCT/US2000/005311 1999-02-26 2000-02-25 Sequences d'acides nucleiques de 9-hydroperoxyde lyase d'acides gras WO2000050575A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12196899P 1999-02-26 1999-02-26
US60/121,968 1999-02-26

Publications (2)

Publication Number Publication Date
WO2000050575A2 WO2000050575A2 (fr) 2000-08-31
WO2000050575A3 true WO2000050575A3 (fr) 2001-09-13

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PCT/US2000/005311 WO2000050575A2 (fr) 1999-02-26 2000-02-25 Sequences d'acides nucleiques de 9-hydroperoxyde lyase d'acides gras

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WO (1) WO2000050575A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6271018B1 (en) * 2000-03-29 2001-08-07 Vanderbilt University Muskmelon (Cucumis melo) hydroperoxide lyase and uses thereof
CA2400585C (fr) 2000-12-18 2008-10-28 Ulrike Schafer Region regulatrice de la peroxyde lyase
FR2825578A1 (fr) * 2001-06-07 2002-12-13 Rhobio Sur-expression d'une lipoxygenase dans les plantes et diminution de la sensibilite des plantes aux maladies et aux agressions par des organismes pathogenes
FR2825580B1 (fr) * 2001-06-07 2004-07-23 Rhobio Sur-expression d'une lipoxygenase dans les plantes et diminution de la sensibilite des plantes aux maladies et aux agressions par des organismes pathogenes
US7317012B2 (en) 2005-06-17 2008-01-08 Bristol-Myers Squibb Company Bicyclic heterocycles as cannabinoind-1 receptor modulators

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000627A2 (fr) * 1998-06-26 2000-01-06 Calgene Llc Sequences d'acides nucleiques d'hydroperoxyde lyase d'acides gras

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000000627A2 (fr) * 1998-06-26 2000-01-06 Calgene Llc Sequences d'acides nucleiques d'hydroperoxyde lyase d'acides gras

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
BATE N J ET AL: "Molecular characterization of an Arabidopsis gene encoding hydroperoxide lyase, a cytochrome P-450 that is wound inducible", PLANT PHYSIOLOGY,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 117, no. 4, August 1998 (1998-08-01), pages 1393 - 1400, XP002128564, ISSN: 0032-0889 *
BATE NICHOLAS J ET AL: "Quantitative and qualitative differences in C6-volatile production from the lipoxygenase pathway in an alcohol dehydrogenase mutant of Arabidopsis thaliana.", PHYSIOLOGIA PLANTARUM, vol. 104, no. 1, 1998, pages 97 - 104, XP000950009, ISSN: 0031-9317 *
CROFT KEVAN P C ET AL: "Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv. phaseolicola.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 101, no. 1, 1993, pages 13 - 24, XP000946545, ISSN: 0032-0889 *
DATABASE GENEMBL 14 May 1999 (1999-05-14), SHELDRICK ET AL.: "Lycopersicum esculentum partial mRNA for hydroperoxide layse", XP002136823 *
DATABASE GENEMBL 17 April 2000 (2000-04-17), MATSUI ET AL.: "Cucumis sativa fatty acid hydroperoxide lyase mRNA, complete cds", XP002148901 *
FAUCONNIER ET AL: "Purification and characterization of tomato leaf (Lycopersicon esculentum Mill.) hydroperoxide lyase", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY. WASHINGTON, vol. 45, no. 11, November 1997 (1997-11-01), pages 4232 - 4236, XP002126015, ISSN: 0021-8561 *
GARGOURI MOHAMED ET AL: "Biosynthesis and analysis of 3Z-nonenal.", BIOTECHNOLOGY LETTERS, vol. 20, no. 1, January 1998 (1998-01-01), pages 23 - 26, XP000949969, ISSN: 0141-5492 *
HATANAKA A ET AL: "EXPRESSION OF LIPOXYGENASE AND HYDROPEROXIDE LYASE ACTIVITIES IN TOMATO FRUITS", ZEITSCHRIFT FÜR NATURFORSCHUNG, vol. 47, no. 5/06, 1992, pages 369 - 374, XP002120138 *
HORNOSTAJ A R ET AL: "Purification of hydroperoxide lyase from cucumbers.", FOOD CHEMISTRY, vol. 66, no. 2, August 1999 (1999-08-01), pages 173 - 180, XP000949974, ISSN: 0308-8146 *
KIM I-S ET AL: "PARTIAL PURIFICATION AND PROPERTIES OF A HYDRO PER OXIDE LYASE FROM FRUITS OF PEAR PYRUS-COMMUNIS", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 29, no. 6, 1981, pages 1220 - 1225, XP000946489, ISSN: 0021-8561 *
MATSUI ET AL.: "Fatty acid 9- and 13-hydroperoxide lyases from cucumber", FEBS LETTERS, vol. 481, no. 2, 15 September 2000 (2000-09-15), pages 183 - 188, XP000952042 *
MATSUI ET AL: "Bell pepper fruit fatty acid hydroperoxide lyase is a cytochrome P450 (CYP74B)", FEBS LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 394, no. 1, 23 September 1996 (1996-09-23), pages 21 - 24, XP002126014, ISSN: 0014-5793 *
MATSUI K ET AL: "MOLECULAR CLONING AND EXPRESSION OF ARABIDOPSIS FATTY ACID HYDROPEROXIDE LYASE", PLANT AND CELL PHYSIOLOGY, vol. 40, no. 5, May 1999 (1999-05-01), pages 477 - 481, XP000870044, ISSN: 0032-0781 *
MATSUI K ET AL: "SEPARATION OF 13 AND 9 HYDROPEROXIDE LYASE ACTIVITIES IN COTYLEDONS OF CUCUMBER SEEDLINGS", ZEITSCHRIFT FUER NATURFORSCHUNG SECTION C BIOSCIENCES, vol. 44, no. 9-10, 1989, pages 883 - 885, XP000949968, ISSN: 0341-0382 *
NOORDERMEER M A ET AL: "Alfalfa contains substantial 9- hydroperoxide lyase activity and a 3Z:2E-enal isomerase", FEBS LETTERS,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 443, 25 January 1999 (1999-01-25), pages 201 - 204, XP002136824, ISSN: 0014-5793 *
SHIBATA ET AL: "Purification and properties of fatty acid hydroperoxide lyase from green bell pepper fruits", PLANT AND CELL PHYSIOLOGY, vol. 36, no. 1, 1995, pages 147 - 156, XP000949972, ISSN: 0032-0781 *

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