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WO2000049137A3 - Pluripotential cell derived from an embryonic stem cell and a nucleus of a somatic cell - Google Patents

Pluripotential cell derived from an embryonic stem cell and a nucleus of a somatic cell Download PDF

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Publication number
WO2000049137A3
WO2000049137A3 PCT/GB2000/000576 GB0000576W WO0049137A3 WO 2000049137 A3 WO2000049137 A3 WO 2000049137A3 GB 0000576 W GB0000576 W GB 0000576W WO 0049137 A3 WO0049137 A3 WO 0049137A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
nucleus
embryonic stem
pluripotential
somatic
Prior art date
Application number
PCT/GB2000/000576
Other languages
French (fr)
Other versions
WO2000049137B1 (en
WO2000049137A2 (en
Inventor
Peter Andrews
Paul Kemp
Original Assignee
Intercytex Ltd
Peter Andrews
Paul Kemp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intercytex Ltd, Peter Andrews, Paul Kemp filed Critical Intercytex Ltd
Priority to JP2000599863A priority Critical patent/JP2003514509A/en
Priority to CA002371900A priority patent/CA2371900A1/en
Priority to EP00903886A priority patent/EP1153123A2/en
Priority to AU25636/00A priority patent/AU2563600A/en
Publication of WO2000049137A2 publication Critical patent/WO2000049137A2/en
Publication of WO2000049137A3 publication Critical patent/WO2000049137A3/en
Publication of WO2000049137B1 publication Critical patent/WO2000049137B1/en
Priority to HK02102269.8A priority patent/HK1040741A1/en
Priority to HK02102672.9A priority patent/HK1042112A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/04—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from germ cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Transplantation (AREA)
  • Psychiatry (AREA)
  • Obesity (AREA)
  • Urology & Nephrology (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychology (AREA)

Abstract

The invention relates to isolated pluripotential cells, comprising at least part of the cytoplasm derived from an embryonic stem cell/embryonic germ cell and a nucleus of a somatic cell. The invention also relates to methods to prepare such cell and therapeutic composition comprising said cells.
PCT/GB2000/000576 1999-02-20 2000-02-18 Pluripotential cell derived from an embryonic stem cell and a nucleus of a somatic cell WO2000049137A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2000599863A JP2003514509A (en) 1999-02-20 2000-02-18 Pluripotent functional cells-2
CA002371900A CA2371900A1 (en) 1999-02-20 2000-02-18 Pluripotential cell derived from an embryonic stem cell and a nucleus of a somatic cell
EP00903886A EP1153123A2 (en) 1999-02-20 2000-02-18 Pluripotential cells-2
AU25636/00A AU2563600A (en) 1999-02-20 2000-02-18 Pluripotential cells-2
HK02102269.8A HK1040741A1 (en) 1999-02-20 2002-03-26 Pluripotential cells-2
HK02102672.9A HK1042112A1 (en) 1999-02-20 2002-04-10 Pluripotential cells-2

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9903804.4 1999-02-20
GBGB9903804.4A GB9903804D0 (en) 1999-02-20 1999-02-20 Pluripotential cells-2

Publications (3)

Publication Number Publication Date
WO2000049137A2 WO2000049137A2 (en) 2000-08-24
WO2000049137A3 true WO2000049137A3 (en) 2001-03-08
WO2000049137B1 WO2000049137B1 (en) 2001-05-17

Family

ID=10848084

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/000576 WO2000049137A2 (en) 1999-02-20 2000-02-18 Pluripotential cell derived from an embryonic stem cell and a nucleus of a somatic cell

Country Status (7)

Country Link
EP (1) EP1153123A2 (en)
JP (1) JP2003514509A (en)
AU (1) AU2563600A (en)
CA (1) CA2371900A1 (en)
GB (1) GB9903804D0 (en)
HK (2) HK1040741A1 (en)
WO (1) WO2000049137A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002038741A1 (en) * 2000-11-09 2002-05-16 Bresagen Limited Cell reprogramming
CA2461185A1 (en) * 2001-09-21 2003-04-03 Japan Science And Technology Corporation Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs
JP2004248505A (en) * 2001-09-21 2004-09-09 Norio Nakatsuji Undifferentiated fusion cell of somatic cell derived from es cell deficient in part or all of transplantation antigen and method for producing the same
US7510876B2 (en) 2003-12-23 2009-03-31 Cythera, Inc. Definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
SG169359A1 (en) 2006-01-31 2011-03-30 Univ Keio A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
JP5149791B2 (en) 2006-04-28 2013-02-20 第一三共株式会社 Method for inducing differentiation of cardiomyocytes from pluripotent stem cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997007669A1 (en) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Quiescent cell populations for nuclear transfer

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FULKA J ET AL: "CLONING BY SOMATIC CELL NUCLEAR TRANSFER", MICROBIOLOGICAL REVIEWS,US,AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, vol. 20, 1998, pages 847 - 851, XP002900701, ISSN: 0146-0749 *
J A THOMSON ET AL: "EMBRYONIC STEM CELL LINES DERIVED FROM HUMAN BLASTOCYSTS", SCIENCE,AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,,US, vol. 282, 6 November 1998 (1998-11-06), pages 1145 - 1147, XP002121340, ISSN: 0036-8075 *
WANGH LAWRENCE J ET AL: "Efficient reactivation of Xenopus erythrocyte nuclei in Xenopus egg extracts.", JOURNAL OF CELL SCIENCE, vol. 108, no. 6, 1995, pages 2187 - 2196, XP000939319, ISSN: 0021-9533 *
YEOM ET AL: "Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells", DEVELOPMENT,GB,COLCHESTER, ESSEX, vol. 122, 1996, pages 881 - 894, XP002094592, ISSN: 0950-1991 *

Also Published As

Publication number Publication date
CA2371900A1 (en) 2000-08-24
GB9903804D0 (en) 1999-04-14
HK1040741A1 (en) 2002-06-21
EP1153123A2 (en) 2001-11-14
HK1042112A1 (en) 2002-08-02
JP2003514509A (en) 2003-04-22
AU2563600A (en) 2000-09-04
WO2000049137B1 (en) 2001-05-17
WO2000049137A2 (en) 2000-08-24

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