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WO2000044906A3 - Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli - Google Patents

Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli Download PDF

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Publication number
WO2000044906A3
WO2000044906A3 PCT/US2000/002200 US0002200W WO0044906A3 WO 2000044906 A3 WO2000044906 A3 WO 2000044906A3 US 0002200 W US0002200 W US 0002200W WO 0044906 A3 WO0044906 A3 WO 0044906A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
proliferation
screen
proteins
coli
Prior art date
Application number
PCT/US2000/002200
Other languages
English (en)
Other versions
WO2000044906A2 (fr
Inventor
Judith Zyskind
Kari L Ohlsen
John D Trawick
R Allyn Forsyth
Jamie M Froelich
Grant J Carr
Robert T Yamamoto
H Howard Xu
Original Assignee
Elitra Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc filed Critical Elitra Pharmaceuticals Inc
Priority to AU35845/00A priority Critical patent/AU3584500A/en
Priority to EP00914458A priority patent/EP1149166A2/fr
Priority to NZ512835A priority patent/NZ512835A/xx
Priority to KR1020017009547A priority patent/KR20010101861A/ko
Priority to CA002360085A priority patent/CA2360085A1/fr
Priority to JP2000596147A priority patent/JP2002535007A/ja
Publication of WO2000044906A2 publication Critical patent/WO2000044906A2/fr
Publication of WO2000044906A3 publication Critical patent/WO2000044906A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Communicable Diseases (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne des séquences d'acides nucléiques codant pour des protéines qui interviennent dans la prolifération cellulaire de E. coli. Ces acides nucléiques peuvent être utilisés pour exprimer des protéines ou des parties de celles-ci, afin de produire des anticorps capables de se lier spécifiquement aux protéines exprimées, et d'utiliser lesdites protéines exprimées comme crible pour isoler des molécules candidates pour des programmes de découverte de médicaments rationnels. Lesdits acides nucléiques peuvent également être utilisés pour cribler des gènes homologues nécessaires à la prolifération cellulaire dans des micro-organisme autres que E. coli. Les acides nucléiques peuvent, en outre, être utilisés pour concevoir des vecteurs d'expression et des vecteurs de sécrétion. Ils sont également utilisés dans différents systèmes d'analyse afin de cribler des gènes de prolifération requis dans d'autres organismes et pour cribler des agents antimicrobiens.
PCT/US2000/002200 1999-01-27 2000-01-27 Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli WO2000044906A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU35845/00A AU3584500A (en) 1999-01-27 2000-01-27 Genes identified as required for proliferation in escherichia coli
EP00914458A EP1149166A2 (fr) 1999-01-27 2000-01-27 Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli
NZ512835A NZ512835A (en) 1999-01-27 2000-01-27 Genes identified as required for proliferation in escherichia coli
KR1020017009547A KR20010101861A (ko) 1999-01-27 2000-01-27 대장균 증식을 위한 유전자
CA002360085A CA2360085A1 (fr) 1999-01-27 2000-01-27 Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli
JP2000596147A JP2002535007A (ja) 1999-01-27 2000-01-27 Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉの増殖に必要であると同定された遺伝子

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11740599P 1999-01-27 1999-01-27
US60/117,405 1999-01-27

Publications (2)

Publication Number Publication Date
WO2000044906A2 WO2000044906A2 (fr) 2000-08-03
WO2000044906A3 true WO2000044906A3 (fr) 2001-02-01

Family

ID=22372748

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/002200 WO2000044906A2 (fr) 1999-01-27 2000-01-27 Genes identifies intervenant dans la proliferation cellulaire d'escherichia coli

Country Status (7)

Country Link
EP (1) EP1149166A2 (fr)
JP (1) JP2002535007A (fr)
KR (1) KR20010101861A (fr)
AU (1) AU3584500A (fr)
CA (1) CA2360085A1 (fr)
NZ (1) NZ512835A (fr)
WO (1) WO2000044906A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1130094A3 (fr) * 1999-07-08 2001-11-21 Helix Research Institute Amorces pour la synthèse de cADN de pleine longueur et leur utilisation
EP1220903A1 (fr) * 1999-10-13 2002-07-10 Pantheco A/S Selection de genes utilisant des sequences de pna
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
WO2002079467A2 (fr) * 2001-03-29 2002-10-10 Københavns Universitet Selection de souche bacterienne sans antibiotique a l'aide de molecules antisens
US20030082591A1 (en) * 2001-07-24 2003-05-01 Donald Awrey Methods for gene disruption and uses thereof
FR2842210B1 (fr) * 2002-07-09 2012-12-14 Mutabilis Determinants de pathogenicite utilisables comme cibles pour l'elaboration de moyens de prevention et de controle d'infections bacteriennes et/ou de la dissemination systemique
WO2007013411A1 (fr) * 2005-07-25 2007-02-01 Kaneka Corporation Acide nucléique antisens dérivé d'un organisme
US8080632B2 (en) * 2005-09-01 2011-12-20 The Feinstein Institute For Medical Research Peptide mimics of melanocyte stimulating hormone
DK2482836T3 (en) 2009-10-02 2016-06-06 Univ Oslo Hf Antibacterial polypeptides and the use thereof
US9090928B2 (en) 2010-10-07 2015-07-28 Yale University Site-specific incorporation of phosphoserine into proteins in Escherichia coli
US11788111B2 (en) 2011-07-11 2023-10-17 Yale University Compositions and methods for making selenocysteine containing polypeptides
US10876142B2 (en) 2011-07-11 2020-12-29 Yale University Compositions and methods for making selenocysteine containing polypeptides
WO2013009868A1 (fr) 2011-07-11 2013-01-17 Yale University Compositions et procédés de fabrication de polypeptides contenant de la sélénocystéine
US10240158B2 (en) 2011-07-11 2019-03-26 Yale University Compositions and methods for making selenocysteine containing polypeptides
BR112021014805A2 (pt) * 2019-01-31 2022-01-04 Spiber Inc Métodos de produção para uma proteína recombinante e para aumentar o volume de produção
CN113917140B (zh) * 2021-10-28 2023-08-18 生工生物工程（上海）股份有限公司 一种快速筛选原核蛋白表达菌的方法
CN116218890A (zh) * 2021-12-06 2023-06-06 深圳华大生命科学研究院 基因串联表达盒、多位点基因编辑系统及应用
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (fr) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Procede d'identification de genes
WO1999002673A2 (fr) * 1997-07-10 1999-01-21 Genesense Technologies, Inc. Sequences oligonucleotidiques antisens servant d'inhibiteurs de micro-organismes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (fr) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Procede d'identification de genes
WO1999002673A2 (fr) * 1997-07-10 1999-01-21 Genesense Technologies, Inc. Sequences oligonucleotidiques antisens servant d'inhibiteurs de micro-organismes

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AUSTIN A.E. ET AL.: "Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli k12 insertion mutagenesis of the RFA locus", JOURNAL OF BACTERIOLOGY, vol. 172, 1990, WASHINGTON US, pages 5312 - 5325, XP000926028 *
BLATTNER F R ET AL: "THE COMPLETE GENOME SEQUENCE OF ESCHERICHIA COLI K-12", SCIENCE., vol. 277, 5 September 1997 (1997-09-05), LANCASTER, PA., US, pages 1453 - 1462, XP002923023, ISSN: 0036-8075 *
LEE N.G. ET AL.: "Molecular cloning and characterization of the nontypable Haemophilus influenzae-2019 rfaE gene required for lipopolysaccharide biosynthesis", INFECTION AND IMMUNITY., vol. 63, no. 3, 1995, WASHINGTON., US, pages 818 - 824, XP000953326, ISSN: 0019-9567 *
POST L E ET AL: "NUCLEOTIDE SEQUENCE OF THE RIBOSOMAL PROTEIN GENE CLUSTER ADJACENT TO THE GENE FOR RNA POLYMERASE SUBUNIT BETA IN ESCHERICHIA COLI", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA,US,NEW YORK, NY, vol. 76, no. 4, 1 April 1979 (1979-04-01), pages 1697 - 1701, XP000574791 *
VALVANO M.A. ET AL.: "The rfaE gene from Escherichia coli encodes a bifunctional protein involved in biosynthesis of the lipopolysaccharide core precursor ADP-L-glycero-D-manno-heptose.", JOURNAL OF BACTERIOLOGY, vol. 182, January 2000 (2000-01-01), WASHINGTON US, pages 488 - 497, XP000926030 *
VAN HEESWIJK W.C. ET AL.: "The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli", MOLECULAR MICROBIOLOGY, vol. 9, 1993, OXFORD GB, pages 443 - 457, XP000926027 *

Also Published As

Publication number Publication date
KR20010101861A (ko) 2001-11-15
AU3584500A (en) 2000-08-18
NZ512835A (en) 2004-02-27
EP1149166A2 (fr) 2001-10-31
WO2000044906A2 (fr) 2000-08-03
CA2360085A1 (fr) 2000-08-03
JP2002535007A (ja) 2002-10-22

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