WO1999038013A3 - Verfahren zur gleichzeitigen identifizierung von proteinen und ihren bindungspartnern - Google Patents
Verfahren zur gleichzeitigen identifizierung von proteinen und ihren bindungspartnern Download PDFInfo
- Publication number
- WO1999038013A3 WO1999038013A3 PCT/DE1999/000220 DE9900220W WO9938013A3 WO 1999038013 A3 WO1999038013 A3 WO 1999038013A3 DE 9900220 W DE9900220 W DE 9900220W WO 9938013 A3 WO9938013 A3 WO 9938013A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- proteins
- combinatory
- bank
- isolated
- bonding partners
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 13
- 102000004169 proteins and genes Human genes 0.000 title abstract 13
- 238000000034 method Methods 0.000 title abstract 2
- 108010058683 Immobilized Proteins Proteins 0.000 abstract 1
- 238000001311 chemical methods and process Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Die Erfindung betrifft Verfahren zur gleichzeitigen Identifizierung eines Proteins und seines Bindungspartners, das dadurch gekennzeichnet ist, daß man a) Proteine oder Proteinaggregate aus einer biologischen Quelle isoliert und auftrennt; b) die aufgetrennten Proteine oder Proteinaggregate auf einer Oberfläche immobilisiert; c) eine kombinatorische Bank mit den auf einer Oberfläche immobilisierten Proteinen oder Proteinaggregaten inkubiert; d) diejenigen Mitglieder der kombinatorischen Bank, die an die immobilisierten Proteine binden, von nicht-gebundenen Mitgliedern der Bank trennt; e) die an die Oberfläche gebundenen Komplexe aus Protein und Bindungspartner aus der kombinatorischen Bank isoliert; f) die Proteine in den so isolierten Komplexen mit einer Kombination aus einem physikalisch-chemischen Verfahren identifiziert; und g) gegebenenfalls die isolierten Bindungspartner anreichert. Die vorliegende Erfindung erlaubt somit die gleichzeitige Identifizierung von Proteinen mit und ohne vorherige Reinigung, sowie die Auswahl von Mitgliedern kombinatorischer Banken, die mit diesen Proteinen wechselwirken. Dadurch läßt sich auf einfache Weise die Funktion der Proteine über ihre spezifischen Bindungspartner ermitteln.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU29199/99A AU2919999A (en) | 1998-01-23 | 1999-01-22 | Method for simultaneous identification of proteins and bonding partners |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19802576.9 | 1998-01-23 | ||
| DE1998102576 DE19802576B4 (de) | 1998-01-23 | 1998-01-23 | Verfahren zur gleichzeitigen Identifizierung von Proteinen und ihren Bindungspartnern |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1999038013A2 WO1999038013A2 (de) | 1999-07-29 |
| WO1999038013A3 true WO1999038013A3 (de) | 1999-10-14 |
Family
ID=7855501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/DE1999/000220 WO1999038013A2 (de) | 1998-01-23 | 1999-01-22 | Verfahren zur gleichzeitigen identifizierung von proteinen und ihren bindungspartnern |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2919999A (de) |
| DE (1) | DE19802576B4 (de) |
| WO (1) | WO1999038013A2 (de) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6377732B1 (en) | 1999-01-22 | 2002-04-23 | The Whitaker Corporation | Planar waveguide devices and fiber attachment |
| AU2001253462A1 (en) * | 2000-04-13 | 2001-11-12 | Thermo Finnigan, Llc | Proteomic analysis by parallel mass spectrometry |
| EP2330219A3 (de) | 2000-04-14 | 2011-11-23 | Metabolon, Inc. | Verfahren zur Arzneimittelentdeckung, Krankheitsbehandlung und Diagnose mithilfe eines Metaboloms |
| US7329489B2 (en) | 2000-04-14 | 2008-02-12 | Matabolon, Inc. | Methods for drug discovery, disease treatment, and diagnosis using metabolomics |
| SE0001670D0 (sv) * | 2000-05-04 | 2000-05-04 | Forskarpatent I Syd Ab | Mass-spectrometry-based biosensor |
| IL137905A0 (en) * | 2000-08-16 | 2001-10-31 | Nathan Citri | Direct selection of protein complexes relevant to the physiological or diagnostic context of a biological specimen |
| AU2002216614A1 (en) | 2000-10-03 | 2002-04-15 | Minerva Biotechnologies Corporation | Electronic detection of interaction based on the interruption of flow |
| DE10054055A1 (de) | 2000-10-31 | 2002-05-23 | Nmi Univ Tuebingen | Verfahren zur Analyse von Proteinen |
| AU2002219971A1 (en) * | 2000-11-01 | 2002-05-15 | Minerva Biotechnologies Corporation | Detection of binding species with colloidal and non-colloidal structures |
| DE60111906D1 (de) * | 2000-11-07 | 2005-08-18 | Advanced Gene Technology Corp | Verfahren zum Screenen von Pflanzenextrakten nach Wirkstoffen |
| DE10157336B4 (de) * | 2000-11-24 | 2007-07-19 | Bioserv Analytik Und Medizinprodukte Gmbh | Verfahren zur Herstellung eines Testsystems und Diagnoseverfahren zur Erkennung von Pankreasfunktionsstörungen |
| DE60117637D1 (de) * | 2001-06-05 | 2006-05-04 | Advanced Gene Technology Corp | Chip mit Pflanzenextrakten auf der Oberfläche |
| US7618788B2 (en) | 2002-05-10 | 2009-11-17 | Millipore Corporation | Proteome epitope tags and methods of use thereof in protein modification analysis |
| US7460960B2 (en) | 2002-05-10 | 2008-12-02 | Epitome Biosystems, Inc. | Proteome epitope tags and methods of use thereof in protein modification analysis |
| US20050255491A1 (en) * | 2003-11-13 | 2005-11-17 | Lee Frank D | Small molecule and peptide arrays and uses thereof |
| US7855057B2 (en) | 2006-03-23 | 2010-12-21 | Millipore Corporation | Protein splice variant/isoform discrimination and quantitative measurements thereof |
| US8849577B2 (en) | 2006-09-15 | 2014-09-30 | Metabolon, Inc. | Methods of identifying biochemical pathways |
| EP3701066B1 (de) * | 2017-10-23 | 2024-10-16 | Nautilus Subsidiary, Inc. | Verfahren und systeme zur proteinidentifikation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995031723A1 (en) * | 1994-05-11 | 1995-11-23 | Lars Frykberg | PHAGE/PHAGEMID DISPLAY LIBRARY COMPRISING RANDOMLY CLEAVED cDNA/GENOMIC DNA OR CLONED GENE |
| WO1998015833A1 (en) * | 1996-10-08 | 1998-04-16 | Universiteit Utrecht | Methods and means for selecting peptides and proteins having specific affinity for a target |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5143854A (en) * | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
-
1998
- 1998-01-23 DE DE1998102576 patent/DE19802576B4/de not_active Expired - Fee Related
-
1999
- 1999-01-22 WO PCT/DE1999/000220 patent/WO1999038013A2/de active Application Filing
- 1999-01-22 AU AU29199/99A patent/AU2919999A/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995031723A1 (en) * | 1994-05-11 | 1995-11-23 | Lars Frykberg | PHAGE/PHAGEMID DISPLAY LIBRARY COMPRISING RANDOMLY CLEAVED cDNA/GENOMIC DNA OR CLONED GENE |
| WO1998015833A1 (en) * | 1996-10-08 | 1998-04-16 | Universiteit Utrecht | Methods and means for selecting peptides and proteins having specific affinity for a target |
Also Published As
| Publication number | Publication date |
|---|---|
| DE19802576A1 (de) | 1999-09-09 |
| AU2919999A (en) | 1999-08-09 |
| WO1999038013A2 (de) | 1999-07-29 |
| DE19802576B4 (de) | 2004-10-28 |
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