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WO1999023250A3 - Spezifisches und sensitives nukleinsäurenachweisverfahren - Google Patents

Spezifisches und sensitives nukleinsäurenachweisverfahren Download PDF

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Publication number
WO1999023250A3
WO1999023250A3 PCT/EP1998/006961 EP9806961W WO9923250A3 WO 1999023250 A3 WO1999023250 A3 WO 1999023250A3 EP 9806961 W EP9806961 W EP 9806961W WO 9923250 A3 WO9923250 A3 WO 9923250A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequence
nucleic acid
bind
specific
nucleic acids
Prior art date
Application number
PCT/EP1998/006961
Other languages
English (en)
French (fr)
Other versions
WO1999023250A2 (de
Inventor
Christoph Kessler
Gerd Haberhausen
Knut Bartl
Henrik Orum
Original Assignee
Roche Diagnostics Gmbh
Christoph Kessler
Gerd Haberhausen
Knut Bartl
Henrik Orum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19748690A external-priority patent/DE19748690A1/de
Priority claimed from DE19814001A external-priority patent/DE19814001A1/de
Priority claimed from DE19814828A external-priority patent/DE19814828A1/de
Application filed by Roche Diagnostics Gmbh, Christoph Kessler, Gerd Haberhausen, Knut Bartl, Henrik Orum filed Critical Roche Diagnostics Gmbh
Priority to EP98965653A priority Critical patent/EP1029084A2/de
Priority to CA002308762A priority patent/CA2308762A1/en
Priority to JP2000519105A priority patent/JP2002505071A/ja
Priority to AU21521/99A priority patent/AU741141B2/en
Publication of WO1999023250A2 publication Critical patent/WO1999023250A2/de
Publication of WO1999023250A3 publication Critical patent/WO1999023250A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Gegenstand der Erfindung ist ein Verfahren zum Nachweis einer Nukleinsäure enthaltend die Schritte Herstellung einer Vielzahl von Amplifikaten eines Teilstücks dieser Nukleinsäure mit einer Länge von weniger als 100 Nukleotiden mit Hilfe zweier Primer, von denen einer an eine erste Bindesequenz (A) eines Strangs der Nukleinsäure binden kann und von denen der andere an eine zweite Bindesequenz (C'), die zu einer mit (A) nicht überlappenden, in 3'-Richtung von (A) gelegenen Sequenz (C) im wesentlichen komplementär ist, binden kann, in Anwesenheit einer Sonde mit einer Bindesequenz (D), welche an die zwischen den Sequenzen (A) und (C) gelegene dritte Sequenz (B) oder das Komplement (B') davon binden kann, wobei diese Sonde eine Reportergruppe und eine Quenchergruppe enthält, unter Verwendung einer Polymerase mit 5'-Nukleaseaktivität und Nachweis der Nukleinsäure durch Messung eines Signals, welches durch die Freisetzung der Reportergruppe bedingt ist.
PCT/EP1998/006961 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren WO1999023250A2 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP98965653A EP1029084A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
CA002308762A CA2308762A1 (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method
JP2000519105A JP2002505071A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
AU21521/99A AU741141B2 (en) 1997-11-04 1998-11-03 Specific and sensitive method for detecting nucleic acids

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE19748690A DE19748690A1 (de) 1997-11-04 1997-11-04 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19748690.8 1997-11-04
DE19814001.0 1998-03-28
DE19814001A DE19814001A1 (de) 1998-03-28 1998-03-28 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828A DE19814828A1 (de) 1998-04-02 1998-04-02 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828.3 1998-04-02

Publications (2)

Publication Number Publication Date
WO1999023250A2 WO1999023250A2 (de) 1999-05-14
WO1999023250A3 true WO1999023250A3 (de) 1999-07-22

Family

ID=27217887

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/EP1998/006951 WO1999024606A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
PCT/EP1998/006952 WO1999023249A2 (de) 1997-11-04 1998-11-03 Spezifisches und empfindliches nukleinsäurenachweisverfahren
PCT/EP1998/006961 WO1999023250A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren

Family Applications Before (2)

Application Number Title Priority Date Filing Date
PCT/EP1998/006951 WO1999024606A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren
PCT/EP1998/006952 WO1999023249A2 (de) 1997-11-04 1998-11-03 Spezifisches und empfindliches nukleinsäurenachweisverfahren

Country Status (6)

Country Link
US (1) US7105318B2 (de)
EP (3) EP1029084A2 (de)
JP (3) JP2002509694A (de)
AU (3) AU2152099A (de)
CA (3) CA2308762A1 (de)
WO (3) WO1999024606A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
ES2246546T3 (es) * 1997-12-12 2006-02-16 Digene Corporation Valoracion de enfermedades relacionadas con el virus de papiloma humano.
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7807802B2 (en) 2002-11-12 2010-10-05 Abbott Lab Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae
WO2008080029A2 (en) 2006-12-21 2008-07-03 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US8617816B2 (en) * 2007-03-16 2013-12-31 454 Life Sciences, A Roche Company System and method for detection of HIV drug resistant variants
GB0714389D0 (en) 2007-07-21 2007-09-05 Barry Callebaut Ag Process and product
EP2262911B1 (de) * 2008-04-17 2016-10-12 QIAGEN Gaithersburg, Inc. Zusammensetzungen, verfahren und kits mit synthetischen sonden zum nachweis der anwesenheit einer target-nukleinsäure
US8288520B2 (en) 2008-10-27 2012-10-16 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and system
WO2010088292A1 (en) * 2009-01-28 2010-08-05 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
EP2425019B1 (de) * 2009-05-01 2014-03-19 QIAGEN Gaithersburg, Inc. Ungezieltes verstärkungsverfahren zur erkennung von rna-spleissformen in einer probe
EP2449132B1 (de) 2009-07-01 2015-05-13 Gen-Probe Incorporated Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
AU2010291990B2 (en) 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
CN102822189B (zh) 2010-01-29 2016-06-29 奇亚根盖瑟斯堡股份有限公司 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物
AU2011210748A1 (en) 2010-01-29 2012-08-09 Qiagen Gaithersburg, Inc. Method of determining and confirming the presence of HPV in a sample
WO2011146629A2 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
WO2011149897A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
CN103597095B (zh) 2011-02-24 2016-08-17 奇亚根盖瑟斯堡股份有限公司 用于检测hpv核酸的材料和方法
JP2024097096A (ja) * 2021-04-28 2024-07-18 バイオニクス株式会社 光学測定装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229701A2 (de) * 1986-01-10 1987-07-22 F. Hoffmann-La Roche Ag Nachweis von Viren durch Verstärkung und Hybridisation
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr
WO1995002690A1 (en) * 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5527898A (en) * 1988-09-09 1996-06-18 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES286841Y (es) 1985-05-20 1988-10-01 Vileda Gmbh Dispositivo de acoplamiento de un mango a un util de limpieza
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
KR100242252B1 (ko) 1989-07-11 2000-03-02 다니엘 엘. 캐시앙 핵산서열의 증폭방법
US5453355A (en) * 1990-01-26 1995-09-26 Abbott Laboratories Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CZ288720B6 (cs) * 1991-05-08 2001-08-15 Chiron Corporation Nukleová kyselina, kompozice s jejím obsahem, způsob tvorby hybridizačního produktu a způsob detekce genotypů HCV
ATE161291T1 (de) 1991-08-27 1998-01-15 Hoffmann La Roche Verfahren und reagenzien zum nachweis von hepatitis c
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
DE69733282T2 (de) * 1996-06-04 2006-01-19 University Of Utah Research Foundation, Salt Lake City Überwachung der Hybridisierung während PCR
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
DE69942444D1 (de) * 1998-02-04 2010-07-15 Life Technologies Corp Bestimmung des genotyps eines amplifikationsproduktes an mehreren allelen stellen
US5948648A (en) * 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0229701A2 (de) * 1986-01-10 1987-07-22 F. Hoffmann-La Roche Ag Nachweis von Viren durch Verstärkung und Hybridisation
US5527898A (en) * 1988-09-09 1996-06-18 Hoffmann-La Roche Inc. Detection of human papillomavirus by the polymerase chain reaction
WO1991010675A1 (en) * 1990-01-19 1991-07-25 Stichting Researchfonds Pathologie Primers and process for detecting human papillomavirus genotypes by pcr
WO1995002690A1 (en) * 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe

Also Published As

Publication number Publication date
CA2308762A1 (en) 1999-05-14
JP2002509694A (ja) 2002-04-02
JP2002505071A (ja) 2002-02-19
US7105318B2 (en) 2006-09-12
EP1029083A2 (de) 2000-08-23
AU741141B2 (en) 2001-11-22
EP1029084A2 (de) 2000-08-23
JP2001521765A (ja) 2001-11-13
WO1999024606A2 (de) 1999-05-20
CA2312779A1 (en) 1999-05-20
AU1232099A (en) 1999-05-24
AU2152099A (en) 1999-05-31
WO1999024606A3 (de) 1999-07-22
WO1999023249A3 (de) 1999-09-10
CA2308368A1 (en) 1999-05-14
WO1999023249A2 (de) 1999-05-14
AU2152199A (en) 1999-05-24
WO1999023250A2 (de) 1999-05-14
EP1029077A2 (de) 2000-08-23
US20030175765A1 (en) 2003-09-18
CA2308368C (en) 2009-01-20

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