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WO1999009180A3 - Procede de production enzymatique de guanosindiphosphat-6-desoxyhexoses et leur utilisation pour la production d'oligosaccharides - Google Patents

Procede de production enzymatique de guanosindiphosphat-6-desoxyhexoses et leur utilisation pour la production d'oligosaccharides Download PDF

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Publication number
WO1999009180A3
WO1999009180A3 PCT/EP1998/005242 EP9805242W WO9909180A3 WO 1999009180 A3 WO1999009180 A3 WO 1999009180A3 EP 9805242 W EP9805242 W EP 9805242W WO 9909180 A3 WO9909180 A3 WO 9909180A3
Authority
WO
WIPO (PCT)
Prior art keywords
producing
mannose
gdp
desoxyhexoses
enzymatically
Prior art date
Application number
PCT/EP1998/005242
Other languages
German (de)
English (en)
Other versions
WO1999009180A2 (fr
Inventor
Wolfgang Piepersberg
Juergen Distler
Christoph Albermann
Original Assignee
Roche Diagnostics Gmbh
Wolfgang Piepersberg
Juergen Distler
Christoph Albermann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Wolfgang Piepersberg, Juergen Distler, Christoph Albermann filed Critical Roche Diagnostics Gmbh
Priority to JP2000509844A priority Critical patent/JP2001514896A/ja
Priority to EP98943894A priority patent/EP1005554A2/fr
Publication of WO1999009180A2 publication Critical patent/WO1999009180A2/fr
Publication of WO1999009180A3 publication Critical patent/WO1999009180A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un procédé destiné à la production enzymatique de GDP-6-désoxyhexoses à partir d'un GDP-D-mannose, d'un mannose-1-phosphate ou d'un mannose-6-phosphate en présence d'enzymes adéquats tels qu'une GDP-D-mannose-4,6-déhydratase et éventuellement une GDP-4-kéto-6-désoxy-D-mannose-3,5-épimérase-4-réductase ou une GDP-4-kéto-6-désoxy-D-mannose-4-réductase. L'invention concerne également un procédé permettant de coupler les hexoses activés par GDP obtenus à des oligosaccharides ou polysaccharides par des glycosyltransférases, p.ex. des fucsyltransférases.
PCT/EP1998/005242 1997-08-19 1998-08-18 Procede de production enzymatique de guanosindiphosphat-6-desoxyhexoses et leur utilisation pour la production d'oligosaccharides WO1999009180A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2000509844A JP2001514896A (ja) 1997-08-19 1998-08-18 グアノシン二リン酸−６−デオキシヘキソースの酵素的生産方法およびオリゴ糖製造のためのその使用
EP98943894A EP1005554A2 (fr) 1997-08-19 1998-08-18 Procede de production enzymatique de guanosindiphosphat-6-desoxyhexoses et leur utilisation pour la production d'oligosaccharides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19735994.9 1997-08-19
DE1997135994 DE19735994A1 (de) 1997-08-19 1997-08-19 Verfahren zur enzymatischen Synthese von Guanosindiphosphat-6-desoxyhexosen und deren Verwendung zur Herstellung von Oligosacchariden

Publications (2)

Publication Number Publication Date
WO1999009180A2 WO1999009180A2 (fr) 1999-02-25
WO1999009180A3 true WO1999009180A3 (fr) 1999-04-15

Family

ID=7839464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/005242 WO1999009180A2 (fr) 1997-08-19 1998-08-18 Procede de production enzymatique de guanosindiphosphat-6-desoxyhexoses et leur utilisation pour la production d'oligosaccharides

Country Status (4)

Country Link
EP (1) EP1005554A2 (fr)
JP (1) JP2001514896A (fr)
DE (1) DE19735994A1 (fr)
WO (1) WO1999009180A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6500661B1 (en) 1998-01-15 2002-12-31 Neose Technologies, Inc. Enzymatic conversion of GDP-mannose to GDP-fucose
FR2796082B1 (fr) * 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
US6875591B1 (en) * 1999-08-10 2005-04-05 Kyowa, Hakko Kogyo Co., Ltd. Process for producing GDP-fucose
JP2001112488A (ja) * 1999-08-10 2001-04-24 Kyowa Hakko Kogyo Co Ltd Ｇｄｐ−フコースの製造法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0395217A2 (fr) * 1989-04-28 1990-10-31 The Biomembrane Institute Synthèse bio-organique de LeX (difucosyle y2; III3FucV3FucnLc6Cer) dimère et leurs analogues
US5728568A (en) * 1996-11-22 1998-03-17 Genetics Institute, Inc. Human GDP-mannose 4,6 dehydratase
EP0870841A1 (fr) * 1996-09-17 1998-10-14 Kyowa Hakko Kogyo Co., Ltd. Procedes de production de nucleotides de sucre et de glucides complexes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0380470B1 (fr) * 1986-03-24 1995-02-15 Texaco Development Corporation Procede de synthese de saccharo-nucleotides par des techniques d'adn recombinant
JP3107847B2 (ja) * 1991-03-29 2000-11-13 寳酒造株式会社 ポリペプチド
US6127153A (en) * 1995-06-07 2000-10-03 Neose Technologies, Inc. Method of transferring at least two saccharide units with a polyglycosyltransferase, a polyglycosyltransferase and gene encoding a polyglycosyltransferase
WO1997037682A1 (fr) * 1996-04-10 1997-10-16 Cytel Corporation Acides nucleiques codant une guanosine diphospho-fucose pyrophosphorylase

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0395217A2 (fr) * 1989-04-28 1990-10-31 The Biomembrane Institute Synthèse bio-organique de LeX (difucosyle y2; III3FucV3FucnLc6Cer) dimère et leurs analogues
EP0870841A1 (fr) * 1996-09-17 1998-10-14 Kyowa Hakko Kogyo Co., Ltd. Procedes de production de nucleotides de sucre et de glucides complexes
US5728568A (en) * 1996-11-22 1998-03-17 Genetics Institute, Inc. Human GDP-mannose 4,6 dehydratase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BONIN C P ET AL: "The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP -D- mannose -4,6- dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (1997 MAR 4) 94 (5) 2085-90. JOURNAL CODE: PV3. ISSN: 0027-8424., United States, XP002087190 *
GORDON STEVENSON ET AL.: "Organization of the Escherichia coli K-12 gene cluster responsible for production of th extracellular polysaccharide colanic acid", JOURNAL OF BACTERIOLOGY, vol. 178, no. 16, August 1996 (1996-08-01), pages 4885 - 4893, XP002087864 *
STROEHER U H ET AL: "A putative pathway for perosamine biosynthesis is the first function encoded within the rfb region of Vibrio cholerae O1", GENE, vol. 166, no. 1, 1 January 1995 (1995-01-01), pages 33-42, XP004043107 *
STURLA L ET AL: "Expression, purification and characterization of GDP -D- mannose 4,6- dehydratase from Escherichia coli.", FEBS LETTERS, (1997 JUL 21) 412 (1) 126-30. JOURNAL CODE: EUH. ISSN: 0014-5793., Netherlands, XP002087189 *
SULIE CHANG ET AL.: "An Epimerase-Reductase in L-Fucose synthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 263, no. 4, 5 February 1988 (1988-02-05), MD US, pages 1693 - 1697, XP002087191 *
SULLIVAN F X ET AL: "Molecular cloning of human GDP - mannose 4,6- dehydratase and reconstitution of GDP-fucose biosynthesis in vitro.", JOURNAL OF BIOLOGICAL CHEMISTRY, (1998 APR 3) 273 (14) 8193-202. JOURNAL CODE: HIV. ISSN: 0021-9258., United States, XP002087192 *

Also Published As

Publication number Publication date
JP2001514896A (ja) 2001-09-18
EP1005554A2 (fr) 2000-06-07
WO1999009180A2 (fr) 1999-02-25
DE19735994A1 (de) 1999-02-25

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