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WO1999007841A3 - Regulatory mutants of adp-glucose pyrophosphorylase and related compositions and methods - Google Patents

Regulatory mutants of adp-glucose pyrophosphorylase and related compositions and methods Download PDF

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Publication number
WO1999007841A3
WO1999007841A3 PCT/US1998/016551 US9816551W WO9907841A3 WO 1999007841 A3 WO1999007841 A3 WO 1999007841A3 US 9816551 W US9816551 W US 9816551W WO 9907841 A3 WO9907841 A3 WO 9907841A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
increased
adp
mutants
related compositions
Prior art date
Application number
PCT/US1998/016551
Other languages
French (fr)
Other versions
WO1999007841A2 (en
Inventor
Thomas Okita
Thomas W Greene
Mary Laughlin
Michael Kahn
Hiroyuki Ito
Original Assignee
Univ Washington
Thomas Okita
Thomas W Greene
Mary Laughlin
Michael Kahn
Hiroyuki Ito
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Washington, Thomas Okita, Thomas W Greene, Mary Laughlin, Michael Kahn, Hiroyuki Ito filed Critical Univ Washington
Priority to AU91972/98A priority Critical patent/AU9197298A/en
Publication of WO1999007841A2 publication Critical patent/WO1999007841A2/en
Publication of WO1999007841A3 publication Critical patent/WO1999007841A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Up-regulated allosteric mutants of plant ADPG-PP enzymes having (1) higher sensitivity to allosteric activators (2) lower sensitivity to allosteric inhibitors; (3) increased starch production; (4) increased yield; (5) increased plant size; (6) increased growth rate and (7) increased number of seeds, and methods for efficiently producing and identifying such mutants.
PCT/US1998/016551 1997-08-07 1998-08-07 Regulatory mutants of adp-glucose pyrophosphorylase and related compositions and methods WO1999007841A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU91972/98A AU9197298A (en) 1997-08-07 1998-08-07 Regulatory mutants of adp-glucose pyrophosphorylase and related compositions andmethods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5541097P 1997-08-07 1997-08-07
US60/055,410 1997-08-07

Publications (2)

Publication Number Publication Date
WO1999007841A2 WO1999007841A2 (en) 1999-02-18
WO1999007841A3 true WO1999007841A3 (en) 1999-06-03

Family

ID=21997607

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/016551 WO1999007841A2 (en) 1997-08-07 1998-08-07 Regulatory mutants of adp-glucose pyrophosphorylase and related compositions and methods

Country Status (2)

Country Link
AU (1) AU9197298A (en)
WO (1) WO1999007841A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030226176A1 (en) 1998-04-03 2003-12-04 Exseed Genetics Llc Plant like starches and the method of making them in hosts
FR2828694B1 (en) * 2001-08-17 2003-12-12 Agronomique Inst Nat Rech USE OF ASSOCIATIONS BETWEEN AT LEAST ONE NUCLEIC SEQUENCE POLYMORPHISM OF THE SH2 GENE AND AT LEAST ONE SEED QUALITY CHARACTERISTIC, IN PLANT SELECTION PROCESSES
EP2275550A3 (en) 2001-12-03 2011-07-06 University of Florida Research Variants of ADP-glucose pyrophosphorylase affecting phosphate sensitivity and other parameters
US8030540B2 (en) 2004-04-21 2011-10-04 Basf Plant Science Gmbh Transgenic corn having enhanced nutritional qualities
CN108728478B (en) * 2017-04-20 2021-08-27 中国科学院分子植物科学卓越创新中心 Method for improving yield and starch property by using hydrogen ion pyrophosphorylase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019806A1 (en) * 1990-06-18 1991-12-26 Monsanto Company Increased starch content in plants
WO1994028149A1 (en) * 1993-05-28 1994-12-08 Monsanto Company Method of improving the quality of stored potatoes
EP0634491A1 (en) * 1993-07-12 1995-01-18 Monsanto Company Modified oil content in seeds

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019806A1 (en) * 1990-06-18 1991-12-26 Monsanto Company Increased starch content in plants
WO1994028149A1 (en) * 1993-05-28 1994-12-08 Monsanto Company Method of improving the quality of stored potatoes
EP0634491A1 (en) * 1993-07-12 1995-01-18 Monsanto Company Modified oil content in seeds

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BALLICORA M A ET AL: "ADENOSINE 5'-DIPHOSPHATE-GLUCOSE PYROPHOSPHORYLASE FROM POTATO TUBER", PLANT PHYSIOLOGY, vol. 109, 1995, pages 245 - 251, XP002060466 *
GIROUX M J ET AL: "A SINGLE GENE MUTATION THAT INCREASES MAIZE SEED WEIGHT", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 93, no. 12, June 1996 (1996-06-01), pages 5824 - 5829, XP000652281 *
GREENE T W ET AL: "MUTAGENESIS OF THE POTATO ADPGLUCOSE PYROPHOSPHORYLASE AND CHARACTERIZATION OF AN ALLOSTERIC MUTANT DEFECTIVE IN 3- PHOSPHOGLYCERATE ACTIVATION", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 93, February 1996 (1996-02-01), pages 1509 - 1513, XP002060467 *
K. BALL AND J. PREISS: "Allosteric sites of the large subunit of the spinach leaf ADPglucose pyrophosphorylase", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 40, 1994, pages 24706 - 24711, XP002097297 *
M.J. LAUGHLIN ET AL.,: "Substrate binding mutants of the higher plant ADP-glucose pyrophosphorylase", PHYTOCHEMISTRY, vol. 47, no. 4, 1 February 1998 (1998-02-01), pages 621 - 629, XP002096425 *
T.W. GREENE ET AL.,: "Aspartic acid 413 is important fro the normal allosteric functioning of ADP-glucose pyrophosphorylase", PLANT PHYSIOLOGY, vol. 112, 1996, pages 1315 - 1320, XP002097296 *
T.W. GREENE ET AL.,: "Generation of up-regulated allosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 17, 1 August 1998 (1998-08-01), pages 10322 - 10327, XP002096426 *

Also Published As

Publication number Publication date
AU9197298A (en) 1999-03-01
WO1999007841A2 (en) 1999-02-18

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