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WO1999006565A3 - Antifreeze proteins, dna and expression systems - Google Patents

Antifreeze proteins, dna and expression systems Download PDF

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Publication number
WO1999006565A3
WO1999006565A3 PCT/CA1998/000745 CA9800745W WO9906565A3 WO 1999006565 A3 WO1999006565 A3 WO 1999006565A3 CA 9800745 W CA9800745 W CA 9800745W WO 9906565 A3 WO9906565 A3 WO 9906565A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
antifreeze
dna
chitinase
expression systems
Prior art date
Application number
PCT/CA1998/000745
Other languages
French (fr)
Other versions
WO1999006565A2 (en
Inventor
Choy Hew
Fei Xiong
Barbara Moffatt
Marilyn Griffith
Original Assignee
Ice Biotech Inc
Choy Hew
Fei Xiong
Barbara Moffatt
Marilyn Griffith
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ice Biotech Inc, Choy Hew, Fei Xiong, Barbara Moffatt, Marilyn Griffith filed Critical Ice Biotech Inc
Priority to CA002299620A priority Critical patent/CA2299620A1/en
Priority to AU86206/98A priority patent/AU8620698A/en
Priority to EP98937376A priority patent/EP1002101A2/en
Publication of WO1999006565A2 publication Critical patent/WO1999006565A2/en
Publication of WO1999006565A3 publication Critical patent/WO1999006565A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

§he winter rye, upon cold-induction or aclimation, produces a family of antifreeze proteins that are similar to pathogen-related proteins. Two of these proteins, both of which are chitinase-like proteins, are cloned using molecular biology techniques and are expressed in bacterial and yeast (Pichia) systems and Arabidopsis thaliana. The recombinant proteins showed both chitinase and antifreeze activities. The invention includes the DNA and protein sequences of the chitinase-like antifreeze proteins, any modifications of the said sequences, the expression of these proteins and their appplication in agriculture, food industry and medicine.
PCT/CA1998/000745 1997-07-31 1998-07-31 Antifreeze proteins, dna and expression systems WO1999006565A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002299620A CA2299620A1 (en) 1997-07-31 1998-07-31 Antifreeze proteins, dna and expression systems
AU86206/98A AU8620698A (en) 1997-07-31 1998-07-31 Antifreeze proteins, dna and expression systems
EP98937376A EP1002101A2 (en) 1997-07-31 1998-07-31 Antifreeze proteins, dna and expression systems

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90387297A 1997-07-31 1997-07-31
US08/903,872 1997-07-31

Publications (2)

Publication Number Publication Date
WO1999006565A2 WO1999006565A2 (en) 1999-02-11
WO1999006565A3 true WO1999006565A3 (en) 1999-06-03

Family

ID=25418191

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1998/000745 WO1999006565A2 (en) 1997-07-31 1998-07-31 Antifreeze proteins, dna and expression systems

Country Status (5)

Country Link
EP (1) EP1002101A2 (en)
CN (1) CN1273604A (en)
AU (1) AU8620698A (en)
CA (1) CA2299620A1 (en)
WO (1) WO1999006565A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000049044A1 (en) * 1999-02-19 2000-08-24 Astrazeneca Ab Expression of helicobacter polypeptides in pichia pastoris
JP3234897B2 (en) * 1999-03-25 2001-12-04 農林水産省北海道農業試験場長 Cold-expressed chitinase gene and method for isolating the same
EP1181865A1 (en) * 2000-08-23 2002-02-27 Universite Catholique De Louvain Cryoprotective solutions
WO2003055320A1 (en) * 2002-01-04 2003-07-10 Unilever Nv Antifreeze proteins in vegetables
EP1556492A4 (en) 2002-09-09 2007-03-28 Genesis Res & Dev Corp Ltd Antifreeze proteins isolated from forage grasses and methods for their use
US7968767B2 (en) 2002-09-09 2011-06-28 Jeroen Demmer Antifreeze proteins isolated from forage grasses and methods for their use
KR100543063B1 (en) * 2004-03-16 2006-01-20 (주)넥스젠 Preparation and isolation of recombinant protein
ES2334741B1 (en) * 2008-06-26 2011-02-17 Consejo Superior De Investigaciones Cientificas (Csic) CRYOPROTECTING PROTEIN WITH CHITINASE ACTIVITY AT LOW TEMPERATURES, PROCEDURE FOR OBTAINING AND APPLICATIONS.
ES2334743B1 (en) * 2008-06-27 2011-02-17 Consejo Superior De Investigaciones Cientificas (Csic) CRYOPROTECTING PROTEIN WITH ACTIVITY 1-3 B - LOW TEMPERATURE GLUCANASE, PROCEDURE FOR OBTAINING AND APPLICATIONS.
CN104975034B (en) * 2015-06-25 2018-11-27 吉林省农业科学院 Application of the sheep's hay chitinase LcChi2 gene in terms of improving plant cold tolerance
WO2017005880A1 (en) * 2015-07-07 2017-01-12 Seqirus UK Limited Influenza potency assays
CN111471082A (en) * 2019-01-24 2020-07-31 华东师范大学 A kind of extraction method of radish seedling apoplast antifreeze protein
BR112023026284A2 (en) * 2021-06-15 2024-03-05 Fraunhofer Ges Forschung PLANT PROTECTION AGENTS BASED ON CHITINOLYTIC ENZYMES
CN116171980A (en) * 2023-01-16 2023-05-30 合肥工业大学 Use of DNA nanostructures as antifreeze agents
CN117164674B (en) * 2023-10-24 2024-02-02 上海水大技术转移有限公司 Antifreeze protein, gene, yeast engineering bacteria and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992022581A1 (en) * 1991-06-13 1992-12-23 University Of Waterloo Cold tolerances in plants
US5633451A (en) * 1995-06-07 1997-05-27 University Of Notre Dame Du Lac Transgenic plants having a nucleic acid sequence encoding a dendroides antifreeze protein
US5633450A (en) * 1984-03-26 1997-05-27 Dna Plant Technology Corporation Chitinase-producing plants
WO1998004148A2 (en) * 1996-07-26 1998-02-05 Unilever N.V. Frozen confectionery products

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5633450A (en) * 1984-03-26 1997-05-27 Dna Plant Technology Corporation Chitinase-producing plants
WO1992022581A1 (en) * 1991-06-13 1992-12-23 University Of Waterloo Cold tolerances in plants
US5633451A (en) * 1995-06-07 1997-05-27 University Of Notre Dame Du Lac Transgenic plants having a nucleic acid sequence encoding a dendroides antifreeze protein
WO1998004148A2 (en) * 1996-07-26 1998-02-05 Unilever N.V. Frozen confectionery products

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 1 November 1994 (1994-11-01), BRYNGELSSON T. ET AL.: "Purification, characterization and cDNA sequence of a basic chitinase from barley infected with powdery mildew.", XP002090241 *
HON W.C. ET AL.: "Antifreeze proteins in winter rye are similylar to pathogenesis-related proteins.", PLANT PHYSIOLOGY, vol. 103, no. 3, 1995, pages 879 - 889, XP002090240 *

Also Published As

Publication number Publication date
CA2299620A1 (en) 1999-02-11
CN1273604A (en) 2000-11-15
WO1999006565A2 (en) 1999-02-11
EP1002101A2 (en) 2000-05-24
AU8620698A (en) 1999-02-22

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