+

WO1999005266A3 - Transfert de noyau entre des especes differentes - Google Patents

Transfert de noyau entre des especes differentes Download PDF

Info

Publication number
WO1999005266A3
WO1999005266A3 PCT/US1998/015387 US9815387W WO9905266A3 WO 1999005266 A3 WO1999005266 A3 WO 1999005266A3 US 9815387 W US9815387 W US 9815387W WO 9905266 A3 WO9905266 A3 WO 9905266A3
Authority
WO
WIPO (PCT)
Prior art keywords
nuclear transfer
trans
donor cells
species nuclear
oocytes
Prior art date
Application number
PCT/US1998/015387
Other languages
English (en)
Other versions
WO1999005266A2 (fr
WO1999005266B1 (fr
Inventor
Neal L First
Tanja Dominko
Maissam Mitalipoua
Original Assignee
Wisconsin Alumni Res Found
Neal L First
Tanja Dominko
Maissam Mitalipoua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, Neal L First, Tanja Dominko, Maissam Mitalipoua filed Critical Wisconsin Alumni Res Found
Priority to AU85875/98A priority Critical patent/AU8587598A/en
Publication of WO1999005266A2 publication Critical patent/WO1999005266A2/fr
Publication of WO1999005266A3 publication Critical patent/WO1999005266A3/fr
Publication of WO1999005266B1 publication Critical patent/WO1999005266B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

On décrit un procédé permettant de produire des embryons clonés par transfert nucléaire à partir de cellules donneuses différenciées. Le procédé consiste à cultiver des cellules donneuses différenciées dans un milieu pauvre en sérum et à utiliser en tant qu'ovocyte récepteur des ovocytes de bovins pour des cellules donneuses provenant d'espèces différentes. On peut utiliser ce procédé pour produire des animaux génétiquement identiques et des animaux transgéniques.
PCT/US1998/015387 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes WO1999005266A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU85875/98A AU8587598A (en) 1997-07-26 1998-07-24 Trans-species nuclear transfer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5310397P 1997-07-26 1997-07-26
US60/053,103 1997-07-26

Publications (3)

Publication Number Publication Date
WO1999005266A2 WO1999005266A2 (fr) 1999-02-04
WO1999005266A3 true WO1999005266A3 (fr) 1999-04-15
WO1999005266B1 WO1999005266B1 (fr) 1999-09-23

Family

ID=21981947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/015387 WO1999005266A2 (fr) 1997-07-26 1998-07-24 Transfert de noyau entre des especes differentes

Country Status (2)

Country Link
AU (1) AU8587598A (fr)
WO (1) WO1999005266A2 (fr)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
CN1230989A (zh) * 1996-08-19 1999-10-06 马萨诸塞大学 通过种间核移植制备胚胎细胞或干细胞样细胞系
US7696404B2 (en) 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
CN1299408A (zh) * 1998-03-02 2001-06-13 马萨诸塞大学 通过异种间的核移植产生的胚细胞或干细胞样细胞系
CA2324009A1 (fr) * 1998-03-16 1999-09-23 Relag Pty Ltd. Transfert de noyau de cellules porcines
CA2334953A1 (fr) * 1999-06-30 2001-01-04 Woo-Suk Hwang Procede d'obtention d'embryons humains clones au moyen de techniques de transplantation inter-especes de noyaux
US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
AU753209B2 (en) * 1999-06-30 2002-10-10 Seoul National University Industry Foundation Method for producing cloned tigers by employing inter-species nuclear transplantation technique
JP3652308B2 (ja) * 1999-06-30 2005-05-25 ソウル ナショナル ユニバーシティー インダストリー ファウンデーション クローンウシの生産方法
MXPA01013450A (es) 1999-06-30 2003-09-04 Advanced Cell Tech Inc Transferencia citoplasmica para celulas receptoras de-diferenciacion.
EP1214404A4 (fr) * 1999-09-14 2003-09-03 Univ Massachusetts Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees et technique destinee a renforcer le developpement embryonnaire par modification genetique de cellules donneuses ou par culture de tissus.
NZ519346A (en) * 1999-12-20 2004-02-27 Univ Massachusetts Embryonic or stem-like cells produced by cross species nuclear transplantation
CA2659945C (fr) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Procedes ameliores de reprogrammation de cellules somatiques animales
WO2013010045A1 (fr) 2011-07-12 2013-01-17 Biotime Inc. Nouveaux procédés et formulations pour thérapie cellulaire orthopédique
RS58255B1 (sr) 2013-04-16 2019-03-29 Regeneron Pharma Ciljana modifikacija genoma pacova
RU2685914C1 (ru) 2013-12-11 2019-04-23 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
LT3080279T (lt) 2013-12-11 2018-10-25 Regeneron Pharmaceuticals, Inc. Būdai ir kompozicijos, skirti genomo tikslinei modifikacijai
MX385689B (es) 2014-06-06 2025-03-18 Regeneron Pharma Métodos y composiciones para modificar un locus dirigido.
SMT201800652T1 (it) 2014-06-26 2019-01-11 Regeneron Pharma Metodi e composizioni per modificazioni genetiche mirate e metodi di utilizzo
ES3013183T3 (en) 2014-11-21 2025-04-11 Regeneron Pharma Methods for targeted genetic modification using paired guide rnas
ES2760508T3 (es) 2014-12-19 2020-05-14 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida a través de la transformación múltiple en una sola etapa
CA2978096C (fr) 2015-03-16 2022-06-28 Regeneron Pharmaceuticals, Inc. Animal non humain presentant une baisse de la fonction des neurones moteurs superieurs et inferieurs et de la perception sensorielle
AU2016270587B2 (en) 2015-05-29 2020-12-24 Regeneron Pharmaceuticals, Inc. Non-human animals having a disruption in a C9ORF72 locus
EP3416479A1 (fr) 2016-02-16 2018-12-26 Regeneron Pharmaceuticals, Inc. Animaux non humains ayant un gène de kynuréninase mutant
MX2018014172A (es) 2016-05-20 2019-08-22 Regeneron Pharma Métodos para romper la tolerancia inmunológica usando múltiples arn guías.
EP3490373B1 (fr) 2016-07-29 2023-10-25 Regeneron Pharmaceuticals, Inc. Souris comprenant des mutations qui conduisent à l'expression de fibrillin-1 tronquee en c-terminal
KR102527979B1 (ko) 2016-09-30 2023-04-28 리제너론 파마슈티칼스 인코포레이티드 C9orf72 유전자좌에서 헥사뉴클레오티드 반복 확장을 갖는 비인간 동물
RU2019125818A (ru) 2017-02-27 2021-03-29 Регенерон Фармасьютикалс, Инк. Модели ретиношизиса на животных, отличных от человека
IL319209A (en) 2017-06-27 2025-04-01 Regeneron Pharma Non-human animals containing a humanized ASGR1 locus
WO2019028032A1 (fr) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Cellules souches embryonnaires de souris transgéniques cas et souris et leurs utilisations
MX2020001177A (es) 2017-07-31 2020-09-25 Regeneron Pharma Animales no humanos reporteros de crispr y usos de los mismos.
EP3585161A1 (fr) 2017-07-31 2020-01-01 Regeneron Pharmaceuticals, Inc. Évaluation de la recombinaison induite par crispr/cas avec un acide nucléique donneur exogène in vivo
CN111163633B (zh) 2017-09-29 2022-09-09 瑞泽恩制药公司 包含人源化ttr基因座的非人类动物及其使用方法
US20190141966A1 (en) 2017-11-10 2019-05-16 Regeneron Pharmaceuticals, Inc. Non-Human Animals Comprising SLC30A8 Mutation And Methods Of Use
SG11202002456WA (en) 2017-11-30 2020-04-29 Regeneron Pharma Non-human animals comprising a humanized trkb locus
AU2019239880B2 (en) 2018-03-19 2023-11-30 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using CRISPR/Cas systems
US20220053741A1 (en) 2018-09-13 2022-02-24 Regeneron Pharmaceuticals, Inc. Complement Factor H Gene Knockout Rat as a Model of C3 Glomerulopathy
CN113423831B (zh) 2018-12-20 2023-03-10 瑞泽恩制药公司 核酸酶介导的重复扩增
CN117178959A (zh) 2019-04-04 2023-12-08 瑞泽恩制药公司 包括人源化凝血因子12基因座的非人动物
CA3137761A1 (fr) 2019-06-04 2020-12-10 Regeneron Pharmaceuticals, Inc. Animaux non humains comprenant un locus ttr humanise ayant une mutation beta-slip et procedes d'utilisation
BR112021022722A2 (pt) 2019-06-07 2022-01-04 Regeneron Pharma Animal não humano, célula de animal não humana, genoma de animal não humano, gene de albumina animal não humana humanizada, vetor de alvejamento, método de avaliação da atividade de um reagente, e, método de otimização da atividade de um reagente
US20200404890A1 (en) 2019-06-27 2020-12-31 Regeneron Pharmaceuticals, Inc. Modeling tdp-43 proteinopathy
WO2021108363A1 (fr) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Régulation à la hausse médiée par crispr/cas d'un allèle ttr humanisé
CA3169272A1 (fr) 2020-01-28 2021-08-05 Regeneron Pharmaceuticals, Inc. Animaux non humains comprenant un locus pnpla3 humanise et procedes d'utilisation
EP4099821A1 (fr) 2020-02-07 2022-12-14 Regeneron Pharmaceuticals, Inc. <smallcaps/>? ? ?klkb1? ? ? ? ?animaux non humains comprenant un locushumanisé et procédés d'utilisation
US20230102342A1 (en) 2020-03-23 2023-03-30 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
EP4171215A2 (fr) 2020-06-26 2023-05-03 Regeneron Pharmaceuticals, Inc. Animaux non humains comprenant un locus d'ace2 humanisé
CN118488784A (zh) 2021-11-04 2024-08-13 瑞泽恩制药公司 包含经修饰的cacng1基因座的非人动物
CN118632622A (zh) 2021-12-08 2024-09-10 瑞泽恩制药公司 突变型肌纤蛋白疾病模型及其用途
CN113943712B (zh) * 2021-12-20 2022-03-22 南京岚轩生物科技有限公司 一种电融合缓冲液、其制备方法及电融合方法
US20250049006A1 (en) 2021-12-20 2025-02-13 C/O Regeneron Pharmaceuticals, Inc. Non-human animals comprising humanized ace2 and tmprss loci
US20250194571A1 (en) 2022-02-07 2025-06-19 Regeneron Pharmaceuticals, Inc. Compositions and methods for defining optimal treatment timeframes in lysosomal disease
KR20240144138A (ko) 2022-02-11 2024-10-02 리제너론 파마슈티칼스 인코포레이티드 4r 타우 표적화제를 스크리닝하기 위한 조성물 및 방법
WO2023235677A1 (fr) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Modèle animal de protéinopathie tdp-43
IL317261A (en) 2022-07-29 2025-01-01 Regeneron Pharma Non-human animals containing a modified transferrin receptor locus
CN119562964A (zh) 2022-08-05 2025-03-04 瑞泽恩制药公司 tdp-43的抗聚集变体
US20240224964A9 (en) 2022-09-29 2024-07-11 Regeneron Pharmaceuticals, Inc. Correction of hepatosteatosis in humanized liver animals through restoration of il6/il6r/gp130 signaling in human hepatocytes
WO2024163650A1 (fr) 2023-02-01 2024-08-08 Regeneron Pharmaceuticals, Inc. Animaux comprenant un locus klhdc7b modifié
WO2025006963A1 (fr) 2023-06-30 2025-01-02 Regeneron Pharmaceuticals, Inc. Procédés et compositions pour augmenter la réparation dirigée par homologie
WO2025122754A1 (fr) 2023-12-07 2025-06-12 Regeneron Pharmaceuticals, Inc. Animaux non humains knockout pour gaa

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1998007841A1 (fr) * 1996-08-19 1998-02-26 University Of Massachusetts Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
WO1998030683A2 (fr) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Transfert nucleaire au moyen de cellules donneuses foetales et adultes differentiees

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1998007841A1 (fr) * 1996-08-19 1998-02-26 University Of Massachusetts Lignees de cellules embryonnaires ou de type souche produites par transplantation nucleaire croisee d'especes
WO1998030683A2 (fr) * 1997-01-10 1998-07-16 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts Transfert nucleaire au moyen de cellules donneuses foetales et adultes differentiees

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHESNE P ET AL: "NUCLEAR TRANSFER IN CATTLE: BIRTH OF CLONED CALVES AND ESTIMATION OF BLASTOMERE TOTIPOTENCY IN MORULAE USED AS A SOURCE OF NUCLEI", LIFE SCIENCES, vol. 316, 1993, pages 487 - 491, XP000197855 *
HEYMAN Y ET AL: "CLONING OF DOMESTIC SPECIES", ANIMAL REPRODUCTION SCIENCE, vol. 42, no. 1/04, 1996, pages 427 - 436, XP000671884 *
SIMS M ET FIRST: "PRODUCTION OF CALVES BY TRANSFER OF NUCLEI FROM CULTURED INNER CELL MASS CELLS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, no. 13, June 1993 (1993-06-01), pages 6143 - 6147, XP000604993 *
STICE S L ET AL: "PLURIPOTENT BOVINE EMBRYONIC CELL LINES DIRECT EMBRYONIC DEVELOPMENT FOLLOWING NUCLEAR TRANSFER", BIOLOGY OF REPRODUCTION, vol. 54, no. 1, January 1996 (1996-01-01), pages 100 - 110, XP000671718 *
WOLFE, B.A. ET AL.: "Embryos produced by the transfer of caprine nuclei to enucleated bovine oocytes are capable of cleavage but not to develop to blastocysts", BIOLOGY OF REPRODUCTION, vol. 50, no. suppl. 1, 1994 - 1972, pages 72, XP002092259 *
ZAWADA, W.M. ET AL.: "Somatic cell cloned transgenic bovine neurons for transplantation in Parkinsonian rats", NATURE MEDICINE, vol. 4, no. 5, May 1998 (1998-05-01), pages 569 - 574, XP002092260 *

Also Published As

Publication number Publication date
WO1999005266A2 (fr) 1999-02-04
WO1999005266B1 (fr) 1999-09-23
AU8587598A (en) 1999-02-16

Similar Documents

Publication Publication Date Title
WO1999005266A3 (fr) Transfert de noyau entre des especes differentes
WO1998030683A3 (fr) Transfert nucleaire au moyen de cellules donneuses foetales et adultes differentiees
AU1595195A (en) Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
EP1017423A4 (fr) Clonage de porcs a l&#39;aide de noyaux donneurs a partir de cellules differenciees
EP0938550A4 (fr) Lignees de cellules a bouton embryonnaire cultive, derivees d&#39;embryons d&#39;ongules
WO2000031237A3 (fr) Procede de clonage de porcins
GB2350619B (en) Sex-specific insemination of mammals with low number of sperm cells
DK1200556T3 (da) Fremgangsmåde til fremstilling af en ikke-differentieret kultur af fugleceller ved hjælp af primordiale könceller
AU4059089A (en) In vitro propagation of embryonic stem cells
CA2263729A1 (fr) Procede d&#39;obtention de mammiferes presentant des proprietes genetiques definies
NZ502712A (en) Medium and method for long term culturing of avian primordial germ cells (PGC) in a tissue culture comprising growth factors
AU695398B2 (en) Method of production of transgenic plants wholly transformed into To generation, from meristems
WO2001068831A3 (fr) Reprogrammation effective de materiel nucleaire chez des mammiferes
NO20006545D0 (no) Fremgangsmåte for in vitro modning av humane kjönnsceller
AU570954B2 (en) Propagation of proliferating cell aggregates
Camous et al. In vitro culture of early bovine embryos with trophoblastic vesicles: cleavage through the block stage, followed by pregnancy after transfer
AU3927599A (en) Source of nuclei for nuclear transfer
AU6292898A (en) Efficient nuclear transfer using primordial germ cells
WO2002045490A3 (fr) Methode de clonage d&#39;animaux
CA2329283A1 (fr) Methodes de reduction des agents adventices et des toxines et reactifs pour culture de cellules ainsi produits
NZ331714A (en) Method of initiating and maturing embryonic tissue from immature conifer embryos using medium comprising amino acids and/or abscisic acid
MX9603037A (es) Materiales y metodos para manejar rechazo hiperagudo en xenotrasplante humano.
JPS5774085A (en) Culture medium for cells originating from lymphocytes
EP0229485A3 (fr) Milieu promoteur de croissance pour culture de cellules et procédé pour la culture de cellules utilisant ce milieu
UA12063A1 (uk) Спосіб культивуваhhя острівцевих клітиh підшлуhкової залози

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: B1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: B1

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 09463276

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载