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WO1999002647A3 - Klonierungsvektoren für die herstellung von adenoviralen minimalviren - Google Patents

Klonierungsvektoren für die herstellung von adenoviralen minimalviren Download PDF

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Publication number
WO1999002647A3
WO1999002647A3 PCT/DE1998/001940 DE9801940W WO9902647A3 WO 1999002647 A3 WO1999002647 A3 WO 1999002647A3 DE 9801940 W DE9801940 W DE 9801940W WO 9902647 A3 WO9902647 A3 WO 9902647A3
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WO
WIPO (PCT)
Prior art keywords
adenoviral
possibly
viruses
inverted terminal
packaging signal
Prior art date
Application number
PCT/DE1998/001940
Other languages
English (en)
French (fr)
Other versions
WO1999002647A2 (de
Inventor
Moritz Hillgenberg
Peter Loeser
Frank Schnieders
Volker Sandig
Michael Strauss
Original Assignee
Hepavec Ag Fuer Gentherapie
Moritz Hillgenberg
Peter Loeser
Frank Schnieders
Volker Sandig
Michael Strauss
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19744768A external-priority patent/DE19744768C2/de
Application filed by Hepavec Ag Fuer Gentherapie, Moritz Hillgenberg, Peter Loeser, Frank Schnieders, Volker Sandig, Michael Strauss filed Critical Hepavec Ag Fuer Gentherapie
Priority to EP98944984A priority Critical patent/EP1003895A2/de
Priority to JP2000502148A priority patent/JP2001509375A/ja
Publication of WO1999002647A2 publication Critical patent/WO1999002647A2/de
Publication of WO1999002647A3 publication Critical patent/WO1999002647A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/38—Vector systems having a special element relevant for transcription being a stuffer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Die Erfindung betrifft Klonierungsvektoren für die Herstellung von Adenoviralen Minimalviren, bestehend aus a) zwei adenoviralen terminalen invertierten Replikationssequenzen (ITR, inverted terminal repeats), die von ab) zwei Schnittstellen für eine Restriktionsendonuklease mit einer länger als 8 Bp langen Erkennungssequenz flankiert werden, und ac) ein adenovirales Verpackungssignal, ad) eine Multiple Klonierungsstelle zur Insertion von therapeutischen DNA-Fragmenten, in welche ggf. zusätzlich nichtkodierende chromosomale Säugetier-DNA einkloniert ist, sowie ae) ggf. eine Erkennungsstelle für eine Rekombinase, die zwischen einer der invertierten terminalen Replikationssequenzen und dem adenoviralen Verpackungssignal liegt, af) ggf. eine Reportergenkassette umrahmen, sowie b) einem bakteriellen Plasmidrückgrat mit Replikationsursprung und bakteriellem Resistenzgen, in das ba) ein Verpackungssignal eines Bakteriophagen einkloniert ist.
PCT/DE1998/001940 1997-07-10 1998-07-06 Klonierungsvektoren für die herstellung von adenoviralen minimalviren WO1999002647A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98944984A EP1003895A2 (de) 1997-07-10 1998-07-06 Klonierungsvektoren für die herstellung von adenoviralen minimalviren
JP2000502148A JP2001509375A (ja) 1997-07-10 1998-07-06 最小アデノウイルスベクターを作製するためのクローニングベクター

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19729571.1 1997-07-10
DE19729571 1997-07-10
DE19744768A DE19744768C2 (de) 1997-07-10 1997-10-10 Klonierungsvektoren für die Herstellung von adenoviralen Minimalviren
DE19744768.6 1997-10-10

Publications (2)

Publication Number Publication Date
WO1999002647A2 WO1999002647A2 (de) 1999-01-21
WO1999002647A3 true WO1999002647A3 (de) 1999-04-15

Family

ID=26038196

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/001940 WO1999002647A2 (de) 1997-07-10 1998-07-06 Klonierungsvektoren für die herstellung von adenoviralen minimalviren

Country Status (3)

Country Link
EP (1) EP1003895A2 (de)
JP (1) JP2001509375A (de)
WO (1) WO1999002647A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020136708A1 (en) 1993-06-24 2002-09-26 Graham Frank L. System for production of helper dependent adenovirus vectors based on use of endonucleases
WO2000049166A2 (en) * 1999-02-18 2000-08-24 Merck & Co., Inc. Production of helper dependent adenovirus vectors based on use of endonucleases
PT1811036E (pt) * 1999-02-22 2011-07-11 Univ Georgetown Imunolipossomas dirigidos por fragmento de anticorpo para distribuição sistémica de genes
DE10006886A1 (de) * 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Nichthumaner helferabhängiger Virusvektor
WO2002022080A2 (en) * 2000-09-15 2002-03-21 Merck & Co., Inc. Enhanced first generation adenovirus vaccines expressing codon optimized hiv1-gag, pol, nef and modifications
US6573092B1 (en) 2000-10-10 2003-06-03 Genvec, Inc. Method of preparing a eukaryotic viral vector
US20050090010A1 (en) * 2001-03-02 2005-04-28 Yoshihide Hayashizaki Cloning vectors and method for molecular cloning
EP1359217B1 (de) * 2002-04-29 2006-12-13 The Trustees of The University of Pennsylvania Methode für die direkte Gewinnung und Amplifikation von integrierten Viren aus zellulärer Gewebe-DNA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023582A1 (en) * 1993-04-08 1994-10-27 Genetic Therapy, Inc. Adenoviral vectors including dna encoding lung surfactant protein

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023582A1 (en) * 1993-04-08 1994-10-27 Genetic Therapy, Inc. Adenoviral vectors including dna encoding lung surfactant protein

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEN H.-H. ET AL.: "Persistance in muscle of an adenoviral vector that lacks all viral genes.", PROC. NATL. ACAD. SCI. USA, vol. 94, March 1997 (1997-03-01), pages 1645 - 1650, XP002092801 *
GHOSH-CHOUDHURY G ET AL: "HUMAN ADENOVIRUS CLONING VECTORS BASED ON INFECTIOUS BACTERIAL PLASMIDS", GENE, vol. 50, no. 1/03, 1 January 1986 (1986-01-01), pages 161 - 171, XP002004335 *
GOSSEN M. ET AL.: "TIGHT CONTROL OF GENE EXPRESSION IN MAMMALIAN CELLS BY TETRACYCLINE-RESPONSIVE PROMOTERS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 89, no. 12, 15 June 1992 (1992-06-15), pages 5547 - 5551, XP000564458 *
HARDY S. ET AL.: "CONSTRUCTION OF ADENOVIRUS VECTORS THROUGH CRE-LOX RECOMBINATION", JOURNAL OF VIROLOGY, vol. 71, no. 3, March 1997 (1997-03-01), pages 1842 - 1849, XP000670223 *
NEERING S. J. ET AL.: "TRANSDUCTION OF PRIMITIVE HUMAN HEMATOPOIETIC CELLS WITH RECOMBINANT ADENOVIRUS VECTORS", BLOOD, vol. 88, no. 4, 15 August 1996 (1996-08-15), pages 1147 - 1155, XP000674228 *
VIRET J.-F.: "MEGANUCLEASE I-SCEL AS A TOOL FOR THE EASY SUBCLONING OF LARGE DNA FRAGMENTS DEVOID OF SELECTION MARKER", BIOTECHNIQUES, vol. 14, no. 3, 1 March 1993 (1993-03-01), pages 325/326, XP000571595 *

Also Published As

Publication number Publication date
JP2001509375A (ja) 2001-07-24
WO1999002647A2 (de) 1999-01-21
EP1003895A2 (de) 2000-05-31

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