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WO1999064583A3 - Compositions et procedes permettant de moduler la degradation proteolytique de cibles intracellulaires - Google Patents

Compositions et procedes permettant de moduler la degradation proteolytique de cibles intracellulaires Download PDF

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Publication number
WO1999064583A3
WO1999064583A3 PCT/US1999/013301 US9913301W WO9964583A3 WO 1999064583 A3 WO1999064583 A3 WO 1999064583A3 US 9913301 W US9913301 W US 9913301W WO 9964583 A3 WO9964583 A3 WO 9964583A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
intracellular targets
proteolytic degradation
binding proteins
Prior art date
Application number
PCT/US1999/013301
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English (en)
Other versions
WO1999064583A2 (fr
Inventor
Mary Jo Mulligan-Kehoe
Original Assignee
Us Health
Mulligan Kehoe Mary Jo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, Mulligan Kehoe Mary Jo filed Critical Us Health
Priority to AU52041/99A priority Critical patent/AU5204199A/en
Publication of WO1999064583A2 publication Critical patent/WO1999064583A2/fr
Publication of WO1999064583A3 publication Critical patent/WO1999064583A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne une protéine à liaison ciblée inactivée, par exemple une cible intracellulaire exemplaire de glucose-6-phosphate déhydrogénase (G6PD), au moyen d'un mécanisme faisant intervenir une dégradation protéosomale. L'invention concerne également des bibliothèques de protéines recombinantes de liaison à affichage de phages, qui renferment des séquences relatives au transport de protéines jusqu'à des protéosomes et à la modulation du taux de protéolyse. De plus, l'invention porte sur des techniques d'inactivation de cibles intracellulaires au moyen de protéines de liaison recombinantes.
PCT/US1999/013301 1998-06-12 1999-06-11 Compositions et procedes permettant de moduler la degradation proteolytique de cibles intracellulaires WO1999064583A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU52041/99A AU5204199A (en) 1998-06-12 1999-06-11 Compositions and methods for modulating proteolytic degradation of intracellulartargets

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9689998A 1998-06-12 1998-06-12
US09/096,899 1998-06-12

Publications (2)

Publication Number Publication Date
WO1999064583A2 WO1999064583A2 (fr) 1999-12-16
WO1999064583A3 true WO1999064583A3 (fr) 2000-02-17

Family

ID=22259627

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/013301 WO1999064583A2 (fr) 1998-06-12 1999-06-11 Compositions et procedes permettant de moduler la degradation proteolytique de cibles intracellulaires

Country Status (2)

Country Link
AU (1) AU5204199A (fr)
WO (1) WO1999064583A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103088135B (zh) * 2013-01-23 2014-12-24 厦门大学 一种葡萄糖-6-磷酸脱氢酶基因突变荧光pcr熔解曲线检测试剂盒
CN117517657B (zh) * 2024-01-08 2024-04-09 中国农业科学院北京畜牧兽医研究所 Lnx1基因或蛋白在调控禽类先天免疫应答反应中的应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002406A2 (fr) * 1986-10-02 1988-04-07 Massachusetts Institute Of Technology Procede de regulation de la stabilite metabolique de proteines
WO1989009829A2 (fr) * 1988-04-07 1989-10-19 Massachusetts Institute Of Technology Procedes de production de residus a terminaison amino dans des proteines
WO1994021680A1 (fr) * 1993-03-17 1994-09-29 The Government Of The United States Of America As Chimeres immunogenes comportant des sequences d'acide nucleique codant des peptides de sequence-signal de reticulum endoplasmique et au moins un autre peptide, et leur utilisation dans les vaccins et le traitement de maladies
WO1995021267A1 (fr) * 1994-02-04 1995-08-10 California Institute Of Technology Module n-degron thermoinductible
WO1996035781A1 (fr) * 1995-05-09 1996-11-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Procede d'inhibition de l'activite d'un constituant intracellulaire
WO1997006685A1 (fr) * 1995-08-18 1997-02-27 Sloan-Kettering Institute For Cancer Research Procede de traitement du cancer et de maladies infectieuses et compositions utiles pour ledit traitement

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002406A2 (fr) * 1986-10-02 1988-04-07 Massachusetts Institute Of Technology Procede de regulation de la stabilite metabolique de proteines
WO1989009829A2 (fr) * 1988-04-07 1989-10-19 Massachusetts Institute Of Technology Procedes de production de residus a terminaison amino dans des proteines
WO1994021680A1 (fr) * 1993-03-17 1994-09-29 The Government Of The United States Of America As Chimeres immunogenes comportant des sequences d'acide nucleique codant des peptides de sequence-signal de reticulum endoplasmique et au moins un autre peptide, et leur utilisation dans les vaccins et le traitement de maladies
WO1995021267A1 (fr) * 1994-02-04 1995-08-10 California Institute Of Technology Module n-degron thermoinductible
WO1996035781A1 (fr) * 1995-05-09 1996-11-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Procede d'inhibition de l'activite d'un constituant intracellulaire
WO1997006685A1 (fr) * 1995-08-18 1997-02-27 Sloan-Kettering Institute For Cancer Research Procede de traitement du cancer et de maladies infectieuses et compositions utiles pour ledit traitement

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
A. VARSHAVSKY: "The N-end rule", CELL, vol. 69, 29 May 1992 (1992-05-29), CELL PRESS,CAMBRIDGE,MA,US;, pages 725 - 735, XP002125276 *
BACHMAIR A ET AL: "THE DEGRADATION SIGNAL IN A SHORT-LIVED PROTEIN", CELL,US,CELL PRESS, CAMBRIDGE, NA, vol. 56, no. 6, pages 1019-1032, XP000050688, ISSN: 0092-8674 *
BARBAS C F ET AL: "ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES: THE GENE III SITE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 88, pages 7978-7982, XP000652426, ISSN: 0027-8424 *
E.S. JOHNSON ET AL.: "Cis-trans recognition and subunit specific degradation of short lived proteins", NATURE, vol. 346, 19 July 1990 (1990-07-19), MACMILLAN JOURNALS LTD., LONDON,UK, pages 287 - 291, XP002125275 *
RECHSTEINER M ET AL: "PEST sequences and regulation by proteolysis", TIBS TRENDS IN BIOCHEMICAL SCIENCES,EN,ELSEVIER PUBLICATION, CAMBRIDGE, vol. 21, no. 7, pages 267-271, XP004050882, ISSN: 0968-0004 *
ROGERS S ET AL: "AMINO ACID SEQUENCES COMMON TO RAPIDLY DEGRADED PROTEINS: THE PEST HYPOTHESIS", SCIENCE,XX,XX, vol. 234, pages 364-368, XP002052319, ISSN: 0036-8075 *
SALAMA S R ET AL: "G1 CYCLIN DEGRADATION: THE PEST MOTIF OF YEAST CLN2 IS NECESSARY, BUT NOT SUFFICIENT, FOR RAPID PROTEIN TURNOVER", MOLECULAR AND CELLULAR BIOLOGY,US,WASHINGTON, DC, vol. 14, no. 12, pages 7953-7966, XP002046707, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
WO1999064583A2 (fr) 1999-12-16
AU5204199A (en) 1999-12-30

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