+

WO1999061623A3 - Genetic method for producing riboflavin - Google Patents

Genetic method for producing riboflavin Download PDF

Info

Publication number
WO1999061623A3
WO1999061623A3 PCT/EP1999/003196 EP9903196W WO9961623A3 WO 1999061623 A3 WO1999061623 A3 WO 1999061623A3 EP 9903196 W EP9903196 W EP 9903196W WO 9961623 A3 WO9961623 A3 WO 9961623A3
Authority
WO
WIPO (PCT)
Prior art keywords
riboflavin
organisms
nucleic acid
seq
genetic method
Prior art date
Application number
PCT/EP1999/003196
Other languages
German (de)
French (fr)
Other versions
WO1999061623A2 (en
Inventor
Henning Althoefer
Harald Seulberger
Oskar Zelder
Doval Jose Luis Revuelta
Original Assignee
Basf Ag
Henning Althoefer
Harald Seulberger
Oskar Zelder
Doval Jose Luis Revuelta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Henning Althoefer, Harald Seulberger, Oskar Zelder, Doval Jose Luis Revuelta filed Critical Basf Ag
Priority to EP99924924A priority Critical patent/EP1082438A2/en
Priority to AU41409/99A priority patent/AU4140999A/en
Priority to JP2000551007A priority patent/JP2002516108A/en
Priority to KR1020007013352A priority patent/KR20010043867A/en
Publication of WO1999061623A2 publication Critical patent/WO1999061623A2/en
Publication of WO1999061623A3 publication Critical patent/WO1999061623A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P25/00—Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a genetic method for producing riboflavin. The production of riboflavin in organisms is increased by specially selecting genes of the riboflavin biosynthesis or of the combination thereof in organisms, especially in bacteria, fungi, yeasts and plants, and of the expression thereof. The invention also relates to a nucleic acid fragment containing genes with the sequences SEQ ID No. 1, SEQ ID No. 3 or SEQ ID No. 5 or the functional equivalents thereof, to expression vectors containing the nucleic acid fragments, and to organisms containing at least one nucleic acid fragment or at least one vector.
PCT/EP1999/003196 1998-05-28 1999-05-10 Genetic method for producing riboflavin WO1999061623A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP99924924A EP1082438A2 (en) 1998-05-28 1999-05-10 Genetic method for producing riboflavin
AU41409/99A AU4140999A (en) 1998-05-28 1999-05-10 Genetic method for producing riboflavin
JP2000551007A JP2002516108A (en) 1998-05-28 1999-05-10 Genetic production of riboflavin
KR1020007013352A KR20010043867A (en) 1998-05-28 1999-05-10 Genetic Method for Producing Riboflavin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19823834.7 1998-05-28
DE19823834A DE19823834A1 (en) 1998-05-28 1998-05-28 Genetic process for the production of riboflavin

Publications (2)

Publication Number Publication Date
WO1999061623A2 WO1999061623A2 (en) 1999-12-02
WO1999061623A3 true WO1999061623A3 (en) 2000-01-27

Family

ID=7869161

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/003196 WO1999061623A2 (en) 1998-05-28 1999-05-10 Genetic method for producing riboflavin

Country Status (9)

Country Link
EP (1) EP1082438A2 (en)
JP (1) JP2002516108A (en)
KR (1) KR20010043867A (en)
CN (1) CN1303434A (en)
AU (1) AU4140999A (en)
DE (1) DE19823834A1 (en)
ID (1) ID27073A (en)
RU (1) RU2000133310A (en)
WO (1) WO1999061623A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU783697B2 (en) 1999-07-01 2005-11-24 Paik Kwang Industrial Co., Ltd. Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
MXPA01012842A (en) 1999-06-25 2002-07-09 Basf Ag Corynebacterium glutamicum.
EP1702980A1 (en) 1999-07-01 2006-09-20 Basf Aktiengesellschaft Corynebacterium glutamicum gene encoding Hpr of phosphoenolpyruvate:sugar phosphotransferase system
US7009045B2 (en) 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
EP1332207B1 (en) * 2000-07-14 2008-10-29 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
DE10046870A1 (en) 2000-09-20 2002-03-28 Basf Ag Genetic manipulation of corynebacteria, useful for preparing fine chemicals, using a non-replicable vector that is not recognized as foreign
CN1298855C (en) 2001-04-04 2007-02-07 金克克国际有限公司 Methods for prodn. of products in host cells
ATE513036T1 (en) 2001-04-04 2011-07-15 Danisco Us Inc UNCOUPLED ANABOLIC AND CATABOLIC METABOLIC PATHWAYS IN HOST CELLS
EP1414962A1 (en) * 2001-07-27 2004-05-06 Basf Aktiengesellschaft Novel genetic products of ashbya gossypii, associated with the mechanisms of signal transduction and especially with the improvement of vitamin b2 production
KR20040027959A (en) * 2001-08-23 2004-04-01 바스프 악티엔게젤샤프트 Novel Metabolism-Associated Gene Products of Ashbya gossypii
DE10159396A1 (en) * 2001-12-04 2003-06-12 Basf Ag Genetic strain optimization for improved production of riboflavin
DE10209363A1 (en) * 2002-03-02 2003-09-11 Basf Ag Process for the preparation of riboflavin
JP5565992B2 (en) * 2005-09-28 2014-08-06 興人ライフサイエンス株式会社 Transformation method of yeast Candida utilis
JP5498651B2 (en) * 2006-07-28 2014-05-21 花王株式会社 Method for producing dipicolinic acid or a salt thereof
CN104531745B (en) * 2014-12-09 2018-05-04 江南大学 A kind of structure of new r plasmid and its utilization in riboflavin produces bacterium
CN113755551A (en) * 2021-09-30 2021-12-07 天津科技大学 A kind of fermentation method for improving riboflavin yield
CN114317557B (en) * 2022-01-06 2023-07-07 河南农业大学 Application of Maize ZmRIBA1 Gene in High Lysine Maize Breeding
CN116463305B (en) * 2023-06-15 2023-10-17 北京易醒生物科技有限公司 Method for improving expression level of alcohol oxidase for ethanol oxidation and optimized riboflavin biosynthesis gene

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0405370A1 (en) * 1989-06-22 1991-01-02 F. Hoffmann-La Roche Ag Riboflavinoverproducing strains of bacteria
WO1993004180A1 (en) * 1991-08-22 1993-03-04 Basf Aktiengesellschaft Process for purposefully modifying the genetic characteristics of ashbya gossypii
EP0569806A2 (en) * 1992-05-11 1993-11-18 BASF Aktiengesellschaft DNA-compounds and recombinant DNA expression vectors coding for S. cerevisiae riboflavine synthetase activity
DE4420785A1 (en) * 1994-03-25 1995-10-05 Basf Ag Riboflavin biosynthesis in fungi
WO1997003208A1 (en) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Riboflavin-production process by means of micro-organisms with modified isocitratlyase activity
EP0821063A2 (en) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Improved riboflavin production

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0405370A1 (en) * 1989-06-22 1991-01-02 F. Hoffmann-La Roche Ag Riboflavinoverproducing strains of bacteria
WO1993004180A1 (en) * 1991-08-22 1993-03-04 Basf Aktiengesellschaft Process for purposefully modifying the genetic characteristics of ashbya gossypii
EP0569806A2 (en) * 1992-05-11 1993-11-18 BASF Aktiengesellschaft DNA-compounds and recombinant DNA expression vectors coding for S. cerevisiae riboflavine synthetase activity
DE4420785A1 (en) * 1994-03-25 1995-10-05 Basf Ag Riboflavin biosynthesis in fungi
WO1997003208A1 (en) * 1995-07-13 1997-01-30 Basf Aktiengesellschaft Riboflavin-production process by means of micro-organisms with modified isocitratlyase activity
EP0821063A2 (en) * 1996-07-24 1998-01-28 F. Hoffmann-La Roche Ag Improved riboflavin production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BROWN D H JR ET AL: "Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme - mediated integration.", MOLECULAR AND GENERAL GENETICS, (1996 APR 24) 251 (1) 75-80., XP002123511 *

Also Published As

Publication number Publication date
KR20010043867A (en) 2001-05-25
JP2002516108A (en) 2002-06-04
RU2000133310A (en) 2002-12-27
ID27073A (en) 2001-02-22
AU4140999A (en) 1999-12-13
CN1303434A (en) 2001-07-11
WO1999061623A2 (en) 1999-12-02
EP1082438A2 (en) 2001-03-14
DE19823834A1 (en) 1999-12-02

Similar Documents

Publication Publication Date Title
WO1999061623A3 (en) Genetic method for producing riboflavin
WO2000032757A3 (en) Plant vitamin e biosynthetic enzymes
WO2003008614A3 (en) Process for the preparation of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced suca or sucb gene
WO2001064898A3 (en) Genes encoding denitrification enzymes
WO2004085607A3 (en) Texturizing lactic bacteria
WO2001019976A3 (en) Plant regulatory sequences for control of gene expression
WO2001016307A3 (en) Plant promoter sequences from maize for selective control of gene expression
WO2003000913A3 (en) Method for the fermentative production of pyruvate
WO1999049058A3 (en) Tryptophan biosynthetic enzymes
WO2000014207A3 (en) Plant farnesyltransferases
WO2000004162A3 (en) Genes encoding sulfate assimilation proteins
AU2002333878A1 (en) A process for the production of l-amino acids using strains of the enterobacteriaceae family which contain an enhanced fadr or iclr gene
WO1997006259A3 (en) Resistance against wilt inducing fungi
CA2173115A1 (en) Genes for butyrobetaine/crotonobetaine-l-carnitine metabolism and their use for the microbiological production of l-carnitine
WO2003070907A3 (en) Novel fungal lipase
WO1999036432A3 (en) Orotidine 5'-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof
WO2000005353A3 (en) Chorismate synthase from plants
AU6727000A (en) Process for producing coenzyme q10
WO2000060087A3 (en) Cyclin dependent kinase inhibitor genes from plants
WO1999048486A3 (en) Plant cell cyclin genes
WO2000028006A3 (en) Plant nadp-specific glutamate dehydrogenase
WO2002050293A3 (en) Plants with improved resistance
WO2000037652A3 (en) Flavonoid biosynthetic enzymes
WO2001034818A3 (en) Sorghum dwarfing genes and methods of use
WO1999049047A3 (en) Plant 1,3-beta-d-glucan synthase and brittle-1 encoding sequences

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99806749.0

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AL AU BG BR BY CA CN CZ GE HU ID IL IN JP KR KZ LT LV MK MX NO NZ PL RO RU SG SI SK TR UA US ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AU BG BR BY CA CN CZ GE HU ID IL IN JP KR KZ LT LV MK MX NO NZ PL RO RU SG SI SK TR UA US ZA

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999924924

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09700454

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 1020007013352

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2000/843/CHE

Country of ref document: IN

WWP Wipo information: published in national office

Ref document number: 1999924924

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1020007013352

Country of ref document: KR

NENP Non-entry into the national phase

Ref country code: CA

WWW Wipo information: withdrawn in national office

Ref document number: 1999924924

Country of ref document: EP

WWR Wipo information: refused in national office

Ref document number: 1020007013352

Country of ref document: KR

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载