+

WO1999061651A3 - Methods for producing a polypeptide by modifying the copy number of a gene - Google Patents

Methods for producing a polypeptide by modifying the copy number of a gene Download PDF

Info

Publication number
WO1999061651A3
WO1999061651A3 PCT/US1999/011816 US9911816W WO9961651A3 WO 1999061651 A3 WO1999061651 A3 WO 1999061651A3 US 9911816 W US9911816 W US 9911816W WO 9961651 A3 WO9961651 A3 WO 9961651A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
polypeptide
acid sequence
cell
methods
Prior art date
Application number
PCT/US1999/011816
Other languages
French (fr)
Other versions
WO1999061651A2 (en
Inventor
Debbie S Yaver
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Priority to AU42139/99A priority Critical patent/AU4213999A/en
Priority to JP2000551031A priority patent/JP2002516116A/en
Priority to EP99925956A priority patent/EP1080210A2/en
Publication of WO1999061651A2 publication Critical patent/WO1999061651A2/en
Publication of WO1999061651A3 publication Critical patent/WO1999061651A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant cell under conditions conducive for production of the polypeptide, wherein (i) the mutant cell is related to a parent cell, which parent cell comprises at least two tandem copies of a nucleic acid sequence encoding the polypeptide or a nucleic acid sequence encoding the polypeptide, which nucleic acid sequence comprises repeat sequences at the 5' and 3' ends of the nucleic acid sequence, by the introduction of a nucleic acid construct into the genome of the parent cell at a locus which is within or not within the nucleic acid sequence(s), wherein the introduction of the nucleic acid construct into the locus modifies the copy number of the nucleic acid sequence(s) and the modification of the copy number is not a result of selective pressure; and (ii) the mutant cell produces more or less of the polypeptide than the parent cell when both cells are cultivated under the same conditions; and (b) recovering the poylpeptide from the cultivation medium. The present invention also relates to methods for obtaining a mutant cell and mutant cells.
PCT/US1999/011816 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene WO1999061651A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU42139/99A AU4213999A (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene
JP2000551031A JP2002516116A (en) 1998-05-27 1999-05-27 Method for producing a polypeptide by changing the copy number of a gene
EP99925956A EP1080210A2 (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8550598A 1998-05-27 1998-05-27
US09/085,505 1998-05-27

Publications (2)

Publication Number Publication Date
WO1999061651A2 WO1999061651A2 (en) 1999-12-02
WO1999061651A3 true WO1999061651A3 (en) 2000-03-02

Family

ID=22192064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011816 WO1999061651A2 (en) 1998-05-27 1999-05-27 Methods for producing a polypeptide by modifying the copy number of a gene

Country Status (5)

Country Link
EP (1) EP1080210A2 (en)
JP (1) JP2002516116A (en)
CN (1) CN1307642A (en)
AU (1) AU4213999A (en)
WO (1) WO1999061651A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2755300A (en) * 1999-02-02 2000-08-25 Novozymes Biotech, Inc. Methods for producing polypeptides in fungal cells
CN1312260A (en) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 Human proteinase regulating protein 10 as one new polypeptide and polynucleotides encoding this polypeptide
WO2002036830A2 (en) * 2000-11-06 2002-05-10 Pepgen Corporation Method of determining gene copy number
ATE460488T1 (en) 2002-04-22 2010-03-15 Novozymes Inc METHOD FOR PRODUCING VARIANTS OF A DNA SEQUENCE IN FILAMENTOUS FUNGI
EP1622921B1 (en) 2003-05-02 2010-06-16 Novozymes Inc. Variants of beta-glucosidases
ATE493489T1 (en) * 2003-08-22 2011-01-15 Suntory Holdings Ltd METHOD FOR GROWING A LIPID PRODUCING MUSHROOM
US20050048619A1 (en) 2003-08-25 2005-03-03 Novozymes Biotech, Inc. Variants of glycoside hydrolases
CN100543141C (en) * 2003-09-16 2009-09-23 周琪 Method for producing gene engineering recombination 192 peptide human growth hormone
DK1682656T3 (en) 2003-10-28 2013-11-18 Novozymes Inc Polypeptides with beta-glucosidase activity and polynucleotides encoding them
ATE489457T1 (en) 2004-01-16 2010-12-15 Novozymes Inc METHOD FOR DEGRADING LIGNOCELLULOSE MATERIALS
DK2314605T3 (en) 2004-01-30 2014-06-16 Novozymes Inc Polypeptides with cellulolytic enhancing activity and polynucleotides encoding them
CN1993475A (en) 2004-05-27 2007-07-04 诺维信股份有限公司 Methods for transforming and expression screening of filamentous fungal cells with a DNA library
WO2006116682A2 (en) 2005-04-27 2006-11-02 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
NZ589570A (en) 2005-09-30 2012-06-29 Novozymes Inc Methods for enhancing the degradation or conversion of cellulosic material
EP2004817A2 (en) 2006-03-30 2008-12-24 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
FR2927089B1 (en) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
ES2526867T3 (en) 2008-11-20 2015-01-16 Novozymes Inc. Polypeptide having amylolytic enhancer activity and polynucleotides encoding it
WO2010080527A1 (en) 2008-12-19 2010-07-15 Novozymes, Inc. Methods for determining cellulolytic enhancing activity of a polypeptide
US8604277B2 (en) 2009-01-28 2013-12-10 Novozymes, Inc. Polypeptides having beta-glucosidase activity and polynucleotides encoding same
US8563268B2 (en) 2009-03-17 2013-10-22 Novozymes A/S Polypeptide having tyrosinase activity
EP2491122A1 (en) 2009-10-23 2012-08-29 Novozymes Inc. Cellobiohydrolase variants and polynucleotides encoding same
CA2780198A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Polypeptides having xylanase activity and polynucleotides encoding same
CN103068976A (en) 2009-11-06 2013-04-24 诺维信股份有限公司 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP2496694B1 (en) 2009-11-06 2017-04-19 Novozymes, Inc. Compositions for saccharification of cellulosic material
ES2594528T3 (en) 2010-09-30 2016-12-20 Novozymes, Inc. Variants of polypeptides with cellulolytic enhancement activity and polynucleotides that encode them
EP2622070B1 (en) 2010-09-30 2016-08-03 Novozymes, Inc. Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US9688975B2 (en) 2010-10-01 2017-06-27 Novozymes, Inc. Beta-glucosidase variants and polynucleotides encoding same
US9676830B2 (en) 2010-11-18 2017-06-13 Novozymes, Inc. Chimeric polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20140080182A1 (en) 2011-03-31 2014-03-20 Novozymes, Inc. Cellulose Binding Domain Variants and Polynucleotides Encoding Same
MX2013012325A (en) 2011-04-28 2013-11-01 Novozymes Inc Polypeptides having endoglucanase activity and polynucleotides encoding same.
BR112013027463A2 (en) 2011-04-29 2017-09-26 Novozymes Inc methods for degrading or converting, and for fermenting a cellulolysis material, for producing a fermentation product, and for cleaning or washing a hard surface or dirty laundry, and detergent composition
US20140329284A1 (en) 2011-09-30 2014-11-06 Novozymes, Inc. Chimeric Polypeptides Having Beta-Glucosidase Activity and Polynucleotides Encoding Same
WO2013096603A2 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
EP4363598A1 (en) * 2021-06-29 2024-05-08 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes in fermentation

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533A1 (en) * 1984-04-27 1985-10-31 Centre Nat Rech Scient METHOD FOR AMPLIFYING EXPRESSION OF A GENE DETERMINED IN BACILLUS SUBTILIS AND OBTAINED STRAINS
WO1988003169A1 (en) * 1986-10-27 1988-05-05 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
EP0343783A2 (en) * 1988-04-22 1989-11-29 Btg Usa Inc Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533A1 (en) * 1984-04-27 1985-10-31 Centre Nat Rech Scient METHOD FOR AMPLIFYING EXPRESSION OF A GENE DETERMINED IN BACILLUS SUBTILIS AND OBTAINED STRAINS
WO1988003169A1 (en) * 1986-10-27 1988-05-05 Whitehead Institute For Biomedical Research Gene amplification using retrotransposons
EP0343783A2 (en) * 1988-04-22 1989-11-29 Btg Usa Inc Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1992001800A1 (en) * 1990-07-20 1992-02-06 Chiron Corporation Method for integrative transformation of yeast using dispersed repetitive elements
WO1995031560A1 (en) * 1994-05-13 1995-11-23 Transkaryotic Therapies, Inc. Dna construct for effecting homologous recombination and uses thereof
WO1998011203A1 (en) * 1996-09-13 1998-03-19 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide

Also Published As

Publication number Publication date
JP2002516116A (en) 2002-06-04
CN1307642A (en) 2001-08-08
EP1080210A2 (en) 2001-03-07
AU4213999A (en) 1999-12-13
WO1999061651A2 (en) 1999-12-02

Similar Documents

Publication Publication Date Title
WO1999061651A3 (en) Methods for producing a polypeptide by modifying the copy number of a gene
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
CN106460000B (en) Targeted mutagenesis
KR920701449A (en) Α-amylase of variant microorganisms with increased stability to temperature, acid and / or alkali
NZ335392A (en) Method for the recombinant production of 1,3-propanediol
WO1999043835A3 (en) Methods for producing a polypeptide in a bacillus cell
IL162302A (en) Methods of producing hyaluronic acid in a bacillus host cell
WO2004090155A3 (en) Methods for producing biological substances in enzyme-deficient mutants of aspergillus niger
DE69939947D1 (en) METHOD OF PREPARING POLYPEPTIDES IN MUTATED ASPERGILLUS CELLS
WO1999060137A3 (en) Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
KR890700675A (en) New proteolytic enzymes
AU3307797A (en) A method for increasing hemoprotein production in filamentous fungi
RU95115686A (en) PREMERSACIDINE, PROMERSACIDINE, DNA, VECTOR, HOST CELL, METHOD FOR PRODUCING
WO1994000463A3 (en) Production of hyaluronic acid by transeformed microorganisms
NZ507607A (en) Method of producing a transgenic plant by transforming witha 1-SST enzyme (sucrose:surcose 1-fructosyltransferase) and/or a 1-FFT enzyme (fructan:fructan 1-fructosyltransferase)
WO2004011609A3 (en) Methods for producing biological substances in pigment-deficient mutants of bacillus cells
WO2000046375A3 (en) Methods for producing polypeptides in fungal cells
NO20052209L (en) Excellence for the production of new and natural avermectin
RU99119890A (en) STREPTOMYCES AVERMITILIS REGULATORY GENES, ENSURING AN INCREASED PRODUCTION OF AVERMECTIN
EP2298911A3 (en) Methods for producing polypeptides in respiratory-deficient cells
JPH05260979A (en) Production of d-ribose
JP2002539834A5 (en)
WO2001051646A3 (en) Methods for producing a polypeptide using a crippled translational initiator sequence
CA2394792A1 (en) Promoter and intron from maize actin depolymerizing factor
AU2857192A (en) Gene coding for esterase and novel microorganism containing said gene

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99808098.5

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AU BA BB BG BR CA CN CU CZ EE GD GE HR HU ID IL IN IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO SG SI SK SL TR TT UA UZ VN YU ZA

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AU BA BB BG BR CA CN CU CZ EE GD GE HR HU ID IL IN IS JP KP KR LC LK LR LT LV MG MK MN MX NO NZ PL RO SG SI SK SL TR TT UA UZ VN YU ZA

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999925956

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2000 551031

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: KR

WWP Wipo information: published in national office

Ref document number: 1999925956

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999925956

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载