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WO1999061581A3 - Clonage, expression et procedes d'utilisation de la sphingosine kinase - Google Patents

Clonage, expression et procedes d'utilisation de la sphingosine kinase Download PDF

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Publication number
WO1999061581A3
WO1999061581A3 PCT/US1999/011521 US9911521W WO9961581A3 WO 1999061581 A3 WO1999061581 A3 WO 1999061581A3 US 9911521 W US9911521 W US 9911521W WO 9961581 A3 WO9961581 A3 WO 9961581A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cloning
expression
sphingosine kinase
sphk
Prior art date
Application number
PCT/US1999/011521
Other languages
English (en)
Other versions
WO1999061581A2 (fr
Inventor
Sarah Spiegel
Original Assignee
Dean Of Res And Graduate Educa
Sarah Spiegel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dean Of Res And Graduate Educa, Sarah Spiegel filed Critical Dean Of Res And Graduate Educa
Priority to EP99924494A priority Critical patent/EP1235913A2/fr
Priority to AU40979/99A priority patent/AU4097999A/en
Priority to CA002329124A priority patent/CA2329124A1/fr
Publication of WO1999061581A2 publication Critical patent/WO1999061581A2/fr
Publication of WO1999061581A3 publication Critical patent/WO1999061581A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne des acides nucléiques codant la sphingosine kinase (SPHK), ainsi que des procédés d'utilisation de celle-ci dans la production de sphingosine-1-phosphate (SPP), dans le criblage des inhibiteurs de SPHK et en tant qu'agent thérapeutique.
PCT/US1999/011521 1998-05-26 1999-05-25 Clonage, expression et procedes d'utilisation de la sphingosine kinase WO1999061581A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99924494A EP1235913A2 (fr) 1998-05-26 1999-05-25 Clonage, expression et procedes d'utilisation de la sphingosine kinase
AU40979/99A AU4097999A (en) 1998-05-26 1999-05-25 Sphingosine kinase, cloning, expression and methods of use
CA002329124A CA2329124A1 (fr) 1998-05-26 1999-05-25 Clonage, expression et procedes d'utilisation de la sphingosine kinase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8665798P 1998-05-26 1998-05-26
US60/086,657 1998-05-26
US9604998P 1998-08-11 1998-08-11
US60/096,049 1998-08-11

Publications (2)

Publication Number Publication Date
WO1999061581A2 WO1999061581A2 (fr) 1999-12-02
WO1999061581A3 true WO1999061581A3 (fr) 2002-07-11

Family

ID=26775005

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/011521 WO1999061581A2 (fr) 1998-05-26 1999-05-25 Clonage, expression et procedes d'utilisation de la sphingosine kinase

Country Status (4)

Country Link
EP (1) EP1235913A2 (fr)
AU (1) AU4097999A (fr)
CA (1) CA2329124A1 (fr)
WO (1) WO1999061581A2 (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6423527B1 (en) 1997-09-29 2002-07-23 Children's Hospital Medical Center Of Northern California Sphingosine-1-phosphate lyase polypeptides, polynucleotides and modulating agents and methods of use therefor
NZ515132A (en) 1999-05-13 2005-01-28 Johnson & Johnson Pharm Res Sphingosine kinase enzyme
AU780746B2 (en) * 1999-05-13 2005-04-14 Johnson & Johnson Pharmaceutical Research & Development Llc Sphingosine kinase enzyme
MXPA02004294A (es) * 1999-10-28 2002-10-31 Warner Lambert Co Gen de esfingosina cinasa de humano.
PL365523A1 (en) * 2000-04-03 2005-01-10 Sankyo Company, Limited Mammalian sphingosine kinase type 2 isoforms, cloning, expression and methods of use thereof
CA2404965A1 (fr) 2000-04-03 2001-10-11 Sankyo Company, Limited Isoformes de la sphingosine kinase mammalienne de type 2, clonage, expression et methodes associees
AUPQ744700A0 (en) * 2000-05-11 2000-06-01 Medvet Science Pty. Ltd. A method of treatment and agents useful for same
WO2002000887A1 (fr) * 2000-06-28 2002-01-03 Medvet Science Pty Ltd Nouveaux variants moleculaires et leurs utilisations
EP1195604A1 (fr) * 2000-09-29 2002-04-10 Warner-Lambert Company Procedés et compositions pour la sondage de modulateurs de lipide kinases
US6610534B2 (en) * 2000-10-05 2003-08-26 Novartis Ag Induction of blood vessel formation through administration of polynucleotides encoding sphingosine kinases
US6858383B2 (en) 2000-12-22 2005-02-22 Medlyte, Inc. Compositions and methods for the treatment and prevention of cardiovascular diseases and disorders, and for identifying agents therapeutic therefor
US20050100547A1 (en) * 2001-06-07 2005-05-12 Pu Xia Etal Sphingosine kinase interacts with traf2 and modulates tumor necrosis factor-induced cellular activity
WO2003014299A2 (fr) * 2001-08-06 2003-02-20 Exelixis, Inc. Sphingosine kinase (sphk) en tant que modificateurs de la voie de passage p53 et methodes d'utilisation
EP1517989B1 (fr) * 2002-01-17 2009-07-15 Children's Hospital & Research Center at Oakland Procédés permettant la détection du métabolisme des sphingolipides
US7674580B2 (en) 2002-01-17 2010-03-09 Children's Hospital & Research Center At Oakland Compositions and methods for the modulation of sphingolipid metabolism and/or signaling
JP2006514828A (ja) * 2002-10-14 2006-05-18 メドベット・サイエンス・プロプライエタリー・リミテッド スフィンゴシンキナーゼの機能的レベルを調節することによって上皮細胞活性を調節する方法
NL1022443C2 (nl) 2003-01-20 2004-07-22 Tno Sphingolipiden voor verbetering van de samenstelling van de darmflora.
ATE427106T1 (de) 2003-01-20 2009-04-15 Tno Die verwendung von sphingolipiden zur senkung der cholesterin- und triglyzeridspiegel im plasma.
EP1670807A1 (fr) * 2003-09-29 2006-06-21 Novartis AG Sphingosines marquees par fluorescence
EP1618876A1 (fr) * 2004-07-19 2006-01-25 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Utilisation de sphingolipides pour la prévention et le traitement de l'athérosclérose
AU2005310341A1 (en) 2004-11-30 2006-06-08 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Sphingolipids in treatment and prevention of steatosis and of steatosis or of hepatotoxicity and its sequelae
EP1910523A4 (fr) * 2005-06-21 2009-05-27 Medvet Science Pty Ltd Modulation de la signalisation de l'egfr par modulation de la signalisation de sphingosine kinase
US20080138334A1 (en) 2006-05-31 2008-06-12 Sabbadini Roger A Immune-Derived Moieties Reactive Against Bioactive Lipids, and Methods of Making and Using Same
US9274130B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Prevention and treatment of pain using antibodies to lysophosphatidic acid
US9274129B2 (en) 2006-05-31 2016-03-01 Lpath, Inc. Methods and reagents for detecting bioactive lipids
CA2667574A1 (fr) 2006-10-27 2008-06-12 Roger A. Sabbadini Compositions et procedes permettant une liaison sphingosine-1-phosphate
US8871202B2 (en) 2008-10-24 2014-10-28 Lpath, Inc. Prevention and treatment of pain using antibodies to sphingosine-1-phosphate

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CUVILLIER OLIVIER ET AL: "Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate.", NATURE (LONDON), vol. 381, no. 6585, 1996, pages 800 - 803, XP002183247, ISSN: 0028-0836 *
DATABASE EMBL [online] 25 June 1997 (1997-06-25), MARRA M. ET AL.: "mp27b05.r1 Life Tech mouse embryo 8 5dpc 10664019 Mus musculus cDNA clone", XP002183249, Database accession no. AA389187 *
DATABASE EMBL [online] 6 November 1996 (1996-11-06), MARRA M. ET AL.: "mp04a08.r1 Life Tech mouse embryo 8 5dpc 10664019 Mus musculus cDNA clone", XP002183248, Database accession no. AA107862 *
DICKSON ROBERT ET AL: "Saccharomyces contain two sphingoid long chain base kinases that define a new family of lipid kinases with the potential to regulate signal transduction pathways and lipid synthesis.", FASEB JOURNAL, vol. 12, no. 8, 24 April 1998 (1998-04-24), Meeting of the American Society for Biochemistry and Molecular Biology;Washington, D.C., USA; May 16-20, 1998, pages A1281, XP002183245, ISSN: 0892-6638 *
KOHAMA ET AL: "Molecular Cloning and Functional Characterization of Murine Sphingosine Kinase", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 37, 11 September 1998 (1998-09-11), pages 23722 - 23728, XP002150781, ISSN: 0021-9258 *
OLIVERA ANA ET AL: "Sphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS mitogens.", NATURE (LONDON), vol. 365, no. 6446, 1993, pages 557 - 560, XP002183246, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO1999061581A2 (fr) 1999-12-02
CA2329124A1 (fr) 1999-12-02
EP1235913A2 (fr) 2002-09-04
AU4097999A (en) 1999-12-13

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