+

WO1999058699A3 - Transgenic plant-derived human blood coagulation factors - Google Patents

Transgenic plant-derived human blood coagulation factors Download PDF

Info

Publication number
WO1999058699A3
WO1999058699A3 PCT/US1999/010732 US9910732W WO9958699A3 WO 1999058699 A3 WO1999058699 A3 WO 1999058699A3 US 9910732 W US9910732 W US 9910732W WO 9958699 A3 WO9958699 A3 WO 9958699A3
Authority
WO
WIPO (PCT)
Prior art keywords
coagulation factors
plant
transgenic plant
human blood
derived human
Prior art date
Application number
PCT/US1999/010732
Other languages
French (fr)
Other versions
WO1999058699A2 (en
Inventor
Brian S Hooker
Jianwei Gao
Daniel B Anderson
Ziyu Dai
Original Assignee
Battelle Memorial Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute filed Critical Battelle Memorial Institute
Priority to CA002328493A priority Critical patent/CA2328493A1/en
Priority to JP2000548490A priority patent/JP2002514433A/en
Priority to EP99921969A priority patent/EP1076698A2/en
Publication of WO1999058699A2 publication Critical patent/WO1999058699A2/en
Publication of WO1999058699A3 publication Critical patent/WO1999058699A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A composition is provided for transgenic plants and transgenic plant derived human coagulation factors capable of eliciting an activation response in human blood clotting pathways and therefore useful for the treatment of human beings diagnosed to be deficient in blood clotting factor proteins. Such proteins may be manufactured by methods resulting in viral free production using both whole plants and plant cell cultures. Also provided are expression vectors for the proper transformation of plant tissue for the production of such factors, as well as transformed plant cells and processes for producing human coagulation factors using plant molecular biology techniques.
PCT/US1999/010732 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors WO1999058699A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002328493A CA2328493A1 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors
JP2000548490A JP2002514433A (en) 1998-05-14 1999-05-14 Human clotting factor from transgenic plants
EP99921969A EP1076698A2 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8000398A 1998-05-14 1998-05-14
US09/080,003 1998-05-14
US30684799A 1999-05-06 1999-05-06
US09/306,847 1999-05-06

Publications (2)

Publication Number Publication Date
WO1999058699A2 WO1999058699A2 (en) 1999-11-18
WO1999058699A3 true WO1999058699A3 (en) 2000-04-06

Family

ID=26762686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010732 WO1999058699A2 (en) 1998-05-14 1999-05-14 Transgenic plant-derived human blood coagulation factors

Country Status (4)

Country Link
EP (1) EP1076698A2 (en)
JP (1) JP2002514433A (en)
CA (1) CA2328493A1 (en)
WO (1) WO1999058699A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2272648C2 (en) * 2000-05-10 2006-03-27 Ново Нордиск Хелт Кэр Аг Pharmaceutical composition comprising factor viia and factor xiii
JP2004505016A (en) * 2000-05-10 2004-02-19 ノボ　ノルディスク　アクティーゼルスカブ Pharmaceutical composition comprising factor VIIa and factor XIII
ES2640613T3 (en) * 2000-07-21 2017-11-03 Revance Therapeutics, Inc. Multi-component biological agent transport systems
DE60231210D1 (en) 2001-07-06 2009-04-02 Chemo Sero Therapeut Res Inst PROCESS FOR THE MANUFACTURE OF HUMAN THROMBIN BY GENE MODIFICATION TECHNOLOGY
EP1446149A1 (en) * 2001-11-09 2004-08-18 Novo Nordisk Health Care AG Pharmaceutical composition comprising factor vii polypeptides and factor v polypeptides
US7125846B2 (en) 2001-11-09 2006-10-24 Novo Nordisk Healthcare A/G Pharmaceutical composition comprising factor VII polypeptides and factor V polypeptides
WO2005123928A1 (en) * 2004-06-08 2005-12-29 Battelle Memorial Institute Production of human coagulation factor viii from plant cells and whole plants
US20070292490A1 (en) * 2004-06-25 2007-12-20 Valentin Negrouk Production of tissue factor in plants
TW202417621A (en) * 2022-10-18 2024-05-01 博唯弘展生技股份有限公司 Production of recombinant human α-thrombin in plant-based system and the application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (en) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression of biologically active factor XIII
WO1991002066A1 (en) * 1989-07-26 1991-02-21 Mogen International N.V. Production of heterologous proteins in plants and plant cells
WO1993013208A1 (en) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Methods for producing thrombin
WO1994017415A1 (en) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Novel anticoagulant cofactor activity
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (en) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression of biologically active factor XIII
WO1991002066A1 (en) * 1989-07-26 1991-02-21 Mogen International N.V. Production of heterologous proteins in plants and plant cells
WO1993013208A1 (en) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Methods for producing thrombin
WO1994017415A1 (en) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Novel anticoagulant cofactor activity
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CRAMER C L ET AL: "Bioproduction of human enzymes in transgenic tobacco.", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, (1996 MAY 25) 792 62-71. REF 50, XP000856387 *
DAI Z Y (REPRINT) ET AL: "Expression of human coagulation factor VIII gene in tobacco.", PLANT PHYSIOLOGY, (JUL 1997) VOL. 114, NO. 3, SUPP. [S], PP. 1584-1584. PUBLISHER: AMER SOC PLANT PHYSIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, M 20855. ISSN: 0032-0889., BATTELLE MEM INST, PACIFIC NW LABS, BIOPROC GRP, RICHLAND, WA 99352, XP002123654 *
KANE W H ET AL: "Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region.", BIOCHEMISTRY, (1990 JUL 24) 29 (29) 6762-8., XP002123653 *

Also Published As

Publication number Publication date
JP2002514433A (en) 2002-05-21
WO1999058699A2 (en) 1999-11-18
EP1076698A2 (en) 2001-02-21
CA2328493A1 (en) 1999-11-18

Similar Documents

Publication Publication Date Title
AR004938A1 (en) PROTEIN DERIVED FROM ALPHA-HORDOTIONINE WITH HIGH METIONIN CONTENT, NUCLEOTIDE, RNA AND DNA SEQUENCES, EXPRESSION CASSETE, BACTERIAL TRANSFORMATION VECTOR, AND TRANSFORMED VEGETABLE METHODS FROM YIDROID, CELLULA, AND CROPS OF TEID
ATE526818T1 (en) GENETICALLY PRODUCED DUSTWASH
EP2169069A3 (en) Phosphatase stress-related proteins and methods of use in plants
IE940815L (en) Plant tissue transformation
SG2013021217A (en) Production of high mannose proteins in plant culture
WO2001045492A3 (en) Protein kinase stress-related proteins and methods of use in plants
WO2003007873A3 (en) Plasma protein matrices and methods for their preparation
WO2001038514A3 (en) Methods, media and apparatus for the introduction of molecules into plant cells and bacteria using aerosol beams
WO2004022696A3 (en) Fibrin cell supports and methods of use thereof
WO1999058699A3 (en) Transgenic plant-derived human blood coagulation factors
SE8800157D0 (en) TRANSFORMATION AND FOREIGN GENE EXPRESSION IN BRASSICA SPECIES
NO20053357L (en) Culture medium, culture method and the obtained myoblasts and their use.
DE60014635D1 (en) METHOD FOR EXPRESSING AND ISOLATING RECOMBINANT PROTEINS AND RECOMBINANT PROTEIN PRODUCTS OF PLANTS, VEGETABLE TISSUES OR CULTIVATED PLANT CELLS
WO2000078927A3 (en) Spheroid preparation
EP1770167A3 (en) Regeneration
CA2275974A1 (en) Methods for producing parthenocarpic or female sterile transgenic plants
JP2004500809A5 (en)
RU2002111666A (en) METHOD FOR PRODUCING PROTEIN SUBSTANCES
CN104911142B (en) A kind of in vitro separation and culture method and application of deer corium hair papilla cell
CN108670942A (en) A kind of anti-ageing anti-wrinkle Essence and preparation method thereof prepared based on stem cells technology
WO1999013083A3 (en) Method and means for modulating plant cell cycle proteins and their use in controlling plant cell growth
ES2132027A1 (en) Development of an artificial skin by means of keratinocyte culture on a base of fibrin and human fibroblasts, and method for preparing this skin for transplantation
WO2004000213A3 (en) Method for growing human organs, treating diseases, and increasing longevity
BR9808472A (en) Method of production of heterologous protein
CA2156181A1 (en) Method for obtaining an increased production of a producing celline by using a fusion protocol

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

ENP Entry into the national phase

Ref document number: 2328493

Country of ref document: CA

Ref country code: CA

Ref document number: 2328493

Kind code of ref document: A

Format of ref document f/p: F

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 548490

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 1999921969

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999921969

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1999921969

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载