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WO1999057993A1 - Utilisation d'enzymes de reticulation de proteine dans des aliments pour ruminants - Google Patents

Utilisation d'enzymes de reticulation de proteine dans des aliments pour ruminants Download PDF

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Publication number
WO1999057993A1
WO1999057993A1 PCT/EP1999/003356 EP9903356W WO9957993A1 WO 1999057993 A1 WO1999057993 A1 WO 1999057993A1 EP 9903356 W EP9903356 W EP 9903356W WO 9957993 A1 WO9957993 A1 WO 9957993A1
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WO
WIPO (PCT)
Prior art keywords
protein
ruminant
feed
enzyme
document
Prior art date
Application number
PCT/EP1999/003356
Other languages
English (en)
Inventor
Robert Franciscus Beudeker
Original Assignee
Dsm N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm N.V. filed Critical Dsm N.V.
Priority to AU42623/99A priority Critical patent/AU4262399A/en
Priority to EP99950323A priority patent/EP1077614A1/fr
Priority to CA002331659A priority patent/CA2331659A1/fr
Publication of WO1999057993A1 publication Critical patent/WO1999057993A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
    • A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants

Definitions

  • rumen-protected amino acids are commercially available, and are used on a large - 3 - scale, the formulation to protect them against rumen proteolysis is an expensive process.
  • gastrointestinal tract of the animals examples comprise phytases, alpha-amylases, proteases and various plant cell wall degrading enzymes.
  • Transglutaminase is added to the meat of domestic fowl
  • transglutaminase has been reviewed recently in Zhu, Y, Rinzema, A., Tramper, and Bol, J. Applied Microbiology
  • transglutaminase is used in vitro to deaminate casein in
  • composition comprising an edible (to the ruminant) protein-containing feed
  • invention aims to cross-link the digestible protein to reduce protein (a nitrogen
  • Protein degradation in silage can be reduced by a rapid drop in pH resulting in an
  • enzymatic proteolytic activity during ensilage can provide a solution to the problem of increasing protein bio-availability to a ruminant.
  • the cross-linking enzyme can be employed as a ruminant feed ingredient or
  • cross-linked proteins in the feed substance can result from the use of the
  • Microbial proteolysis either inside the ruminant (e.g. in the
  • linked protein is degraded by enzymes inside the animal (e.g. proteases) to amino
  • the rumen has high microbial enzyme activity and bacteria are the most important
  • proteases such as serine
  • proteases and metalloproteases, as well as various types of peptidases are involved in
  • the feed substance may be derived from meat (which includes fish) such as meat, bone or fish meal.
  • meat which includes fish
  • the substance may comprise only plant
  • the feed can be devoid of meat and/or fish.
  • silage (fermentated fodder or grass), forage
  • concentrates or compound feed may be free of dairy products, e.g. casein.
  • One can thus provide one or more protein cross-linking enzymes e.g., one or more protein cross-linking enzymes (e.g., one or more protein cross-linking enzymes).
  • transglutaminase as an ingredient for the feed composition, such as silage or
  • the enzyme(s) can be (e.g. naturally)derived from
  • microorganisms plants and animals or can be synthetic or recombinant.
  • enzyme will be in an active form, if present, that is to say it is capable of cross-linking
  • the cross-linking enzyme e.g. transglutaminase
  • the cross-linking enzyme is preferably recombinantly
  • the enzyme may be produced by a (wild-type) strain of e.g. a Streptoverticillium species.
  • a Streptoverticillium species include Streptoverticillium
  • griseocarneum LFO 1277b Streptoverticillium cinnamoneum sub sp. cinnamoneum LFO
  • the enzyme may be produced (e.g. recombinantly by expression of a
  • heterologous gene by a micro-organism such as a bacteria, yeast or fungus (e.g.
  • filamentous fungi Preferably the organism is of the genus Streptomyces, Bacillus,
  • production organisms are E. coli, Streptomyces e.g. Streptomyces livi ⁇ ans, Bacillus e.g.
  • the enzyme can be produced by fermentation
  • transglutaminases One suitable group of protein cross-linking enzymes are the transglutaminases.
  • Transglutaminase (EC 2.3.3.13; protein-glutamine gamma-glutamyltransferase) is an
  • transglutaminase is now possible by cloning a (microbial) gene encoding
  • Protein disulfide isomerase (EC 5.3.4.1), for example,
  • Sulphydryl oxidase (EC 1.8.3.2) or thiol oxidase is capable of catalyzing the oxidation
  • animals such as e.g. mammals, insects, fish, molluscs, nematodes, plants, and
  • the enzyme catalyses the oxidation of phenols to ortho-diphenols
  • ortho-diquinones react with sulphydryl groups or with amine groups to form
  • Lysyl oxidase (EC 1.4.3.13) or lysyl-protein-6-oxidase is an important enzyme in the
  • Peroxidase (EC 1.11.1.7) is capable of catalysing a large number of reactions in
  • linking enzymes other than peroxidase(s) can be used depending on the
  • Lipoxygenase (EC 1.13.11.12, also lipoxydase or linolate:oxygen oxidoreductase)
  • reactive molecules cause protein cross-linking.
  • Glucose oxidase (EC 1.1.3.4) for example, catalyses the oxidation
  • proteins and can be employed although it can be omitted in some embodiments (e.g.
  • the feed substance comprises silage
  • the protein cross-linking enzyme(s) used in the invention will usually be either one
  • enzyme can be added to the feed composition separately from the feed substance(s),
  • the - 10 - enzyme can be an integral part of one of the feed substances.
  • the form of a supplement may be added to the feed substance, e.g. silage, or directly to the feed composition such as after other feed substance(s) have been added or
  • enzyme additive to ruminant feed is to add the enzyme as or in transgenic plant
  • the enzyme may thus have been synthesized through
  • appropriate plant expression signals e.g. a tissue specific promoter, such as a seed
  • the enzyme can be included in the feed, either as such or after further processing.
  • the enzyme can be included in the feed, either as such or after further processing.
  • transgenic plant may be present in a seed of the transgenic plant or it may be present in other plant
  • parts such as roots, stems, leaves, wood, flowers, bark and/or fruit.
  • transglutaminase or other cross-linking enzymes for expression of transglutaminase or other cross-linking enzymes in transgenic plant
  • transgenic plant material e.g. transgenic seed containing the e.g. transglutaminase
  • transgenic seed containing the e.g. transglutaminase may require the processing of the plant
  • processing techniques may include various milling and grinding techniques or
  • thermomechanical treatments such as extrusion or expansion.
  • the protein cross-linking enzyme such as transglutaminase may be added to the feed
  • composition (which includes silage) at concentration which varies as a function of
  • diet composition and target animal species Generally between 1 and 1,000,000 units
  • enzyme per kg protein in the feed composition is suitable.
  • Example 1 Usually the amount of protein in the feed composition is from 15-25%
  • the ruminant is a cow, sheep, deer or goat.
  • the ruminant is a cow, sheep, deer or goat.
  • ruminant is preferably a lactating animal (or at least capable of lactating).
  • Streptomyces lividans expressing the Streptoverticillium transglutaminase gene were selected from the group consisting of Streptomyces lividans expressing the Streptoverticillium transglutaminase gene.
  • transglutaminase activities ranging from 1-10 U/ml.
  • the microorganism was
  • the liquid containing the enzyme was concentrated by means of ultrafiltration (cut
  • unit of enzyme activity is defined as the amount of enzyme catalysing the formation
  • Rumen fluid collection and preparation of rumen protease was as follows.
  • a rumen-fistulated, lactating cow of 700 kg weight was fed on a mixture of maize
  • Rumen fluid was collected 3 hours after morning feeding and sieved through a 1 mm
  • the sieved fluid was centrifuged for 5 min at 1000 g at 4°C to remove solids
  • the supernatant liquid is referred to as rumen protein.
  • the liquid is referred to as rumen protein.
  • crude rumen extracts from the concentrated rumen microorganisms are referred to as crude rumen
  • Rumen protein was analysed for crude rumen protease activity as follows. Rumen
  • transglutaminase resulted in a marked inhibition of rumen protease - 15 activity (Table 1).
  • Negative control (no n.a. 0.3 crude rumen protease, no transglutaminase)
  • Glucose oxidase as a typical example of a protein cross-linking oxidase, was tested - 16 - for its effects on the inhibition of proteolysis in the rumen. Glucose oxidase was
  • Sarett Units per gram One Sarett unit is the amount of enzyme that will cause an
  • Negative control (no n.a. 0.3 crude rumen protease, no glucose oxidase)
  • Forage grass was harvested with a self-propelled metered chopper equipped with grinding plates to damage the kernels.
  • the crop contained 32% dry matter and 24% of this dry matter consisted of crude protein.
  • Transglutaminase was added to the silage at 10 and 10,000 U/kg silage protein by spraying the liquid enzyme on to the crop after wilting while mixing in a concrete mixer. Controls received equal amounts of water. - 18
  • transglutaminase on milk yield and feed conversion ratio (milk/ dry matter intake).
  • Feed conversion ratio was improved by 5% at the higher enzyme dose.
  • Feed conversion ration (milk/dry 1.67 1.71 1.75 matter intake) - 20 -
  • a ruminant feed composition comprising an edible
  • protein-containing feed substance where the protein has been cross-linked and/or the composition additionally comprises a protein cross-linking enzyme.
  • cross-linking enzyme comprises protein disulphide isomerase, protein disulphide
  • reductase sulphydryl oxidase, polyphenol oxidase, lysyl oxidase, peroxidase, glucose
  • protein cross-linking enzyme is derived from an animal, a plant or a microorganism.
  • the protein cross-linking enzyme is of microbial origin and/or is a recombinant
  • the protein cross-linking enzyme comprises a transglutaminase.
  • microorganism is of the genus Streptomyces, Bacillus, Escherichia, Saccharomyces,
  • Penicillium Penicillium, Mucor, Fusarium or Humicola.

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

Des enzymes de réticulation de protéine, telle la transglutaminase, sont utilisées dans des aliments pour ruminants afin d'améliorer l'utilisation de protéines et d'acides aminés par le ruminant. Des aliments ordinaires pour ruminants, tels qu'un ensilage, sont soumis à une protéolyse microbienne à la fois avant d'être apportés à l'animal (en d'autres termes au cours de la fermentation de l'ensilage ou du fourrage), et dans la panse, ce qui a pour conséquence une dégradation de protéines de valeur qui présentent un bénéfice nutritionnel pour le ruminant. Afin de réduire la dégradation de ces protéines et permettre ainsi à une proportion plus élevée de celles-ci d'atteindre le petit intestin du ruminant, les protéines sont réticulées dans les aliments avant que ceux-ci ne soient apportés à l'animal, ou les aliments pour ruminants sont enrichis en une enzyme active de réticulation de protéine.
PCT/EP1999/003356 1998-05-14 1999-05-14 Utilisation d'enzymes de reticulation de proteine dans des aliments pour ruminants WO1999057993A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU42623/99A AU4262399A (en) 1998-05-14 1999-05-14 Use of protein cross-linking enzymes in ruminant feed
EP99950323A EP1077614A1 (fr) 1998-05-14 1999-05-14 Utilisation d'enzymes de reticulation de proteine dans des aliments pour ruminants
CA002331659A CA2331659A1 (fr) 1998-05-14 1999-05-14 Utilisation d'enzymes de reticulation de proteine dans des aliments pour ruminants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98303795 1998-05-14
EP98303795.3 1998-05-14

Publications (1)

Publication Number Publication Date
WO1999057993A1 true WO1999057993A1 (fr) 1999-11-18

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EP (1) EP1077614A1 (fr)
AU (1) AU4262399A (fr)
CA (1) CA2331659A1 (fr)
WO (1) WO1999057993A1 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003007728A3 (fr) * 2001-07-18 2003-12-18 Dsm Ip Assets Bv Denree alimentaire proteique vegetarienne
CN104782900A (zh) * 2015-04-15 2015-07-22 浙江大学 低氮排放的后备奶牛日粮
CN106519010A (zh) * 2016-11-07 2017-03-22 上海海洋大学 黄体生成素重组蛋白饲料添加剂的制备及其使用方法
US20180168192A1 (en) * 2015-08-14 2018-06-21 Russell W. Day Ruminal protection of lipids, lipid-bearing materials, and bioactive aliments
CN108244353A (zh) * 2016-12-28 2018-07-06 上海交通大学 用于促进繁殖母羊氧化还原平衡的饲料添加剂及其应用
US20230180790A1 (en) * 2018-11-21 2023-06-15 Dennis R. Stucker Method for increasing rate and extent of fiber digestion in ruminants
EP4356750A3 (fr) * 2021-07-30 2024-06-19 Glasport Bio Limited Compositions et procédés

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116326686B (zh) * 2022-05-30 2025-02-07 中国科学院东北地理与农业生态研究所 一种改善青贮玉米饲料发酵品质的添加剂及其制备方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5828234A (ja) * 1981-08-08 1983-02-19 Snow Brand Milk Prod Co Ltd タンパク素材の製造方法
EP0108568A1 (fr) * 1982-11-02 1984-05-16 Suomen Sokeri Oy Composition et méthode pour l'ensilage de fourrage et de grain
JPS6167497A (ja) * 1984-09-11 1986-04-07 Ajinomoto Co Inc タンパク素材の製造法
EP0346909A2 (fr) * 1988-06-17 1989-12-20 Cultor Oy Procédé par fermentation et additif pour la conservation de fourrage
JPH067091A (ja) * 1992-04-08 1994-01-18 Ajinomoto Co Inc 栄養価が改良されたタンパク質素材の製造法
JPH06261692A (ja) * 1993-03-18 1994-09-20 Ajinomoto Co Inc 動物用飼料の製造法
AT398362B (de) * 1990-06-06 1994-11-25 Erber Erich Ing Futtermittelzusatz sowie verfahren zu seiner herstellung
JPH08332032A (ja) * 1995-06-12 1996-12-17 Showa Denko Kk 動物経口物用酵素剤及びその用途

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5828234A (ja) * 1981-08-08 1983-02-19 Snow Brand Milk Prod Co Ltd タンパク素材の製造方法
EP0108568A1 (fr) * 1982-11-02 1984-05-16 Suomen Sokeri Oy Composition et méthode pour l'ensilage de fourrage et de grain
JPS6167497A (ja) * 1984-09-11 1986-04-07 Ajinomoto Co Inc タンパク素材の製造法
EP0346909A2 (fr) * 1988-06-17 1989-12-20 Cultor Oy Procédé par fermentation et additif pour la conservation de fourrage
AT398362B (de) * 1990-06-06 1994-11-25 Erber Erich Ing Futtermittelzusatz sowie verfahren zu seiner herstellung
JPH067091A (ja) * 1992-04-08 1994-01-18 Ajinomoto Co Inc 栄養価が改良されたタンパク質素材の製造法
JPH06261692A (ja) * 1993-03-18 1994-09-20 Ajinomoto Co Inc 動物用飼料の製造法
JPH08332032A (ja) * 1995-06-12 1996-12-17 Showa Denko Kk 動物経口物用酵素剤及びその用途

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* Cited by examiner, † Cited by third party
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DATABASE WPI Section Ch Week 8313, Derwent World Patents Index; Class D13, AN 83-30831K, XP002082729 *
DATABASE WPI Section Ch Week 8620, Derwent World Patents Index; Class D13, AN 86-128938, XP002082727 *
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PATENT ABSTRACTS OF JAPAN vol. 018, no. 204 (C - 1189) 11 April 1994 (1994-04-11) *
PATENT ABSTRACTS OF JAPAN vol. 018, no. 662 (C - 1287) 14 December 1994 (1994-12-14) *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003007728A3 (fr) * 2001-07-18 2003-12-18 Dsm Ip Assets Bv Denree alimentaire proteique vegetarienne
CN104782900A (zh) * 2015-04-15 2015-07-22 浙江大学 低氮排放的后备奶牛日粮
US20180168192A1 (en) * 2015-08-14 2018-06-21 Russell W. Day Ruminal protection of lipids, lipid-bearing materials, and bioactive aliments
JP2018522557A (ja) * 2015-08-14 2018-08-16 ラッセル ダブリュー デイＤＡＹ， Ｒｕｓｓｅｌｌ， Ｗ． 脂質、脂質含有材料及び生物活性栄養成分の第一胃内保護
CN106519010A (zh) * 2016-11-07 2017-03-22 上海海洋大学 黄体生成素重组蛋白饲料添加剂的制备及其使用方法
CN108244353A (zh) * 2016-12-28 2018-07-06 上海交通大学 用于促进繁殖母羊氧化还原平衡的饲料添加剂及其应用
US20230180790A1 (en) * 2018-11-21 2023-06-15 Dennis R. Stucker Method for increasing rate and extent of fiber digestion in ruminants
US12171248B2 (en) * 2018-11-21 2024-12-24 Impetus Llc Method for increasing rate and extent of fiber digestion in ruminants
EP4356750A3 (fr) * 2021-07-30 2024-06-19 Glasport Bio Limited Compositions et procédés

Also Published As

Publication number Publication date
CA2331659A1 (fr) 1999-11-18
EP1077614A1 (fr) 2001-02-28
AU4262399A (en) 1999-11-29

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