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WO1999052941A3 - Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses - Google Patents

Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses Download PDF

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Publication number
WO1999052941A3
WO1999052941A3 PCT/GB1999/001096 GB9901096W WO9952941A3 WO 1999052941 A3 WO1999052941 A3 WO 1999052941A3 GB 9901096 W GB9901096 W GB 9901096W WO 9952941 A3 WO9952941 A3 WO 9952941A3
Authority
WO
WIPO (PCT)
Prior art keywords
rho
proteins
gtpases
assays
binding
Prior art date
Application number
PCT/GB1999/001096
Other languages
English (en)
Other versions
WO1999052941B1 (fr
WO1999052941A2 (fr
Inventor
Leland Herries Johnston
Richard Henry Treisman
Original Assignee
Medical Res Council
Imp Cancer Res Tech
Leland Herries Johnston
Richard Henry Treisman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council, Imp Cancer Res Tech, Leland Herries Johnston, Richard Henry Treisman filed Critical Medical Res Council
Priority to AU34326/99A priority Critical patent/AU3432699A/en
Priority to EP99915903A priority patent/EP1068234A2/fr
Publication of WO1999052941A2 publication Critical patent/WO1999052941A2/fr
Publication of WO1999052941A3 publication Critical patent/WO1999052941A3/fr
Publication of WO1999052941B1 publication Critical patent/WO1999052941B1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La famille Rho des GTPases est impliquée dans de nombreux processus cellulaires eukaryotes, certaines protéines effectrices étant connues pour se fixer à ces GTPases, afin de provoquer différents effets, ces protéines étant notamment la ROCK-I/p160ROCK, la ROCKII, et la kinectine. La présente invention concerne également une nouvelle région de fixation à Rho à vingt-trois acides aminés, conservée dans un éventail de protéines de fixation à Rho. D'autres protéines, notamment la protéine de levure Skn7, contiennent des régions homologues à cette région conservée et interagissent avec les Rho GTPases. L'invention concerne enfin des analyses permettant d'identifier les molécules qui altèrent la croissance cellulaire, par une modulation de l'interaction Rho GTPase/protéines effectrices.
PCT/GB1999/001096 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses WO1999052941A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU34326/99A AU3432699A (en) 1998-04-09 1999-04-09 Polypeptide domains capable of binding rho protein and their use in assays
EP99915903A EP1068234A2 (fr) 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9807848.8A GB9807848D0 (en) 1998-04-09 1998-04-09 Assay method and means
GB9807848.8 1998-04-09

Publications (3)

Publication Number Publication Date
WO1999052941A2 WO1999052941A2 (fr) 1999-10-21
WO1999052941A3 true WO1999052941A3 (fr) 1999-12-02
WO1999052941B1 WO1999052941B1 (fr) 2000-01-06

Family

ID=10830272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001096 WO1999052941A2 (fr) 1998-04-09 1999-04-09 Domaines polypeptidiques capables de se fixer aux proteines rho et leur utilisation dans des analyses

Country Status (4)

Country Link
EP (1) EP1068234A2 (fr)
AU (1) AU3432699A (fr)
GB (1) GB9807848D0 (fr)
WO (1) WO1999052941A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1088898A1 (fr) * 1999-09-16 2001-04-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Un nouveau système de détection de substances utiles dans la thérapie du cancer et des maladies infectieuses
EP1254912A1 (fr) * 2001-04-30 2002-11-06 Boehringer Ingelheim International GmbH Polypeptides de CYK-4, ADN molecules condant et leur utilisation pour de procédé de criblage
WO2001097608A2 (fr) * 2000-06-19 2001-12-27 Boehringer Ingelheim International Gmbh Polypeptides cyk-4, molecules d'adn codant ces polypeptides, et utilisation de ceux-ci dans des procedes de criblage
WO2004065959A2 (fr) * 2003-01-23 2004-08-05 Eirx Therapeutics Limited Kinases et recepteurs gpcr impliques dans l'apoptose
FR2862983B1 (fr) * 2003-11-28 2006-04-07 Centre Nat Rech Scient Moyens de criblage pour l'identification de regulateurs de l'activite gtpase

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021252A2 (fr) * 1994-02-01 1995-08-10 The Board Of Trustees Of The Leland Stanford Junior University Peptides derives de wd-40 et leurs utilisations
WO1997005249A2 (fr) * 1995-07-31 1997-02-13 The Government Of The United States Of America, Represented By The Secretary, Department Of Healt H And Human Services Prolongement d'une surface d'interaction proteine-proteine afin d'inactiver le fonctionnement d'une proteine cellulaire
WO1998020127A1 (fr) * 1996-11-06 1998-05-14 Onyx Pharmaceuticals, Inc. Facteur d'echange de guanine de rho gtpase et acide nucleique qui le code
WO1998038331A1 (fr) * 1997-02-28 1998-09-03 Medical Research Council Interactions du gene skn7 et utilisation dudit gene dans des procedes de dosage

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021252A2 (fr) * 1994-02-01 1995-08-10 The Board Of Trustees Of The Leland Stanford Junior University Peptides derives de wd-40 et leurs utilisations
WO1997005249A2 (fr) * 1995-07-31 1997-02-13 The Government Of The United States Of America, Represented By The Secretary, Department Of Healt H And Human Services Prolongement d'une surface d'interaction proteine-proteine afin d'inactiver le fonctionnement d'une proteine cellulaire
WO1998020127A1 (fr) * 1996-11-06 1998-05-14 Onyx Pharmaceuticals, Inc. Facteur d'echange de guanine de rho gtpase et acide nucleique qui le code
WO1998038331A1 (fr) * 1997-02-28 1998-09-03 Medical Research Council Interactions du gene skn7 et utilisation dudit gene dans des procedes de dosage

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BROWN ET AL.: "Putative transcription factor SKN7 (POS9 protein)", SWISSPROT SEQUENCE DATA BASE, 1 February 1995 (1995-02-01), XP002118152 *
BROWN ET AL.: "Yeast Skn7p functions in a eukaryotic two-component regulatory pathway", EMBO J, vol. 13, 1994, pages 5186 - 5194, XP002069763 *
FLEISCHMANN ET AL.: "Hypothetical protein HI1717", SWISSPROT SEQUENCE DATA BASE, 1 November 1995 (1995-11-01), XP002118148 *
LEUNG ET AL.: "Kinectin 1 (coiled-coil protein)", EMBL SEQUENCE DATABASE, 1 November 1996 (1996-11-01), HEIDELBERG DE, XP002118149 *
NAKAGAWA ET AL.: "Rho-associated, coil-coil forming protein kinase P160 ROCK-1", EMBL SEQUENCE DATABASE, 1 February 1997 (1997-02-01), HEIDELBERG DE, XP002118150 *
NAKAGAWA ET AL.: "Rho-associated, coiled-coil forming protein kinase P160 ROCK-2", EMBL SEQUENCE DATABASE, 1 February 1997 (1997-02-01), HEIDELBERG DE, XP002118151 *
NAKAGAWA ET AL.: "ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice", FEBS LETT, vol. 392, 1996, pages 189 - 193 *
SKINNER ET AL.: "Cloning of novel kinectin splice variants with alternative C-termini: structure, distribution and evolution of mouse kinectin", IMMUNOL CELL BIOL, vol. 74, 1996, pages 421 - 433 *

Also Published As

Publication number Publication date
GB9807848D0 (en) 1998-06-10
WO1999052941B1 (fr) 2000-01-06
AU3432699A (en) 1999-11-01
WO1999052941A2 (fr) 1999-10-21
EP1068234A2 (fr) 2001-01-17

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