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WO1999051749A3 - Recombinant potyvirus construct and use thereof - Google Patents

Recombinant potyvirus construct and use thereof Download PDF

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Publication number
WO1999051749A3
WO1999051749A3 PCT/IL1999/000184 IL9900184W WO9951749A3 WO 1999051749 A3 WO1999051749 A3 WO 1999051749A3 IL 9900184 W IL9900184 W IL 9900184W WO 9951749 A3 WO9951749 A3 WO 9951749A3
Authority
WO
WIPO (PCT)
Prior art keywords
construct
potyvirus
plants
present
relates
Prior art date
Application number
PCT/IL1999/000184
Other languages
French (fr)
Other versions
WO1999051749A2 (en
Inventor
Amit Gal-On
Original Assignee
Israel State
Gal On Amit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Israel State, Gal On Amit filed Critical Israel State
Priority to AU30525/99A priority Critical patent/AU3052599A/en
Priority to JP2000542461A priority patent/JP2002536956A/en
Priority to CA002327445A priority patent/CA2327445A1/en
Priority to EP99912037A priority patent/EP1068330A2/en
Publication of WO1999051749A2 publication Critical patent/WO1999051749A2/en
Publication of WO1999051749A3 publication Critical patent/WO1999051749A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/34011—Potyviridae
    • C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a recombinant potyvirus infectious nucleic acid, preferably cDNA or RNA, construct useful for plant cross protection, comprising a full length clone characterized in that its HC-Pro gene conserved FRNK box sequence contains a substitution, preferably a substitution of Arg. Preferably, the construct further contains a substitution which effectively abolishes aphid transmissibility. The present invention further relates to a method for the production of a mild strain of potyvirus utilizing the above mentioned construct and to a method for protecting plants from viral infection and for transient expression of foreign nucleic acid (genes) in plants, using said construct. Preferably, the present invention relates to a method for cross protection of curcurbits against ZYMV infection. The present invention also relates to a method for providing protection against viral infection in plants comprising inoculating plants, by mechanical inoculation or by bombardment, with the recombinant potyvirus infectious construct.
PCT/IL1999/000184 1998-04-07 1999-03-30 Recombinant potyvirus construct and use thereof WO1999051749A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU30525/99A AU3052599A (en) 1998-04-07 1999-03-30 Recombinant potyvirus construct and use thereof
JP2000542461A JP2002536956A (en) 1998-04-07 1999-03-30 Recombinant potyvirus constructs and uses thereof
CA002327445A CA2327445A1 (en) 1998-04-07 1999-03-30 Recombinant potyvirus construct and use thereof
EP99912037A EP1068330A2 (en) 1998-04-07 1999-03-30 Recombinant potyvirus construct and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL123994 1998-04-07
IL12399498 1998-04-07

Publications (2)

Publication Number Publication Date
WO1999051749A2 WO1999051749A2 (en) 1999-10-14
WO1999051749A3 true WO1999051749A3 (en) 1999-12-09

Family

ID=11071404

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL1999/000184 WO1999051749A2 (en) 1998-04-07 1999-03-30 Recombinant potyvirus construct and use thereof

Country Status (7)

Country Link
EP (1) EP1068330A2 (en)
JP (1) JP2002536956A (en)
CN (1) CN1303433A (en)
AU (1) AU3052599A (en)
CA (1) CA2327445A1 (en)
TR (1) TR200002909T2 (en)
WO (1) WO1999051749A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108823174B (en) * 2018-06-28 2021-07-20 山东农业大学 Development and Application of Attenuated Vaccine of Papaya Ringspot Virus Watermelon Strain

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001034823A2 (en) * 1999-11-12 2001-05-17 University Of South Carolina Method for enhancing resistance in plants
EP1425395A4 (en) * 2000-11-28 2006-03-01 Virogene Ltd Systems and methods for delivering interferon to a subject
EP1364035A4 (en) * 2001-01-24 2005-05-04 Virogene Ltd Vectors capable of imparting herbicide resistance and viral cross protection and methods
JP2002238574A (en) * 2001-02-16 2002-08-27 Japan International Research Center For Agricultural Services Full-length genomic RNA of papaya malformed leaf mosaic virus
KR101103705B1 (en) * 2007-12-26 2012-01-11 서울여자대학교 산학협력단 Highly Infectious Cayenne Pepper Virus Nucleic Acid Molecules and Plant Virus Vector Derived from the
CN102277376B (en) * 2010-06-10 2013-09-25 叶锡东 Gene transfer vector providing crop broad-spectrum virus resistance and containing pawpaw ringspot virus helper component protease gene and application thereof
EP2514304A1 (en) * 2011-04-20 2012-10-24 Syngenta Participations AG Cucurbita plant resistant to potyvirus
CN102379305B (en) * 2011-09-28 2013-11-20 中国农业大学 Novel applications of corn ferredoxin
CN102796739B (en) * 2012-08-16 2014-02-19 北京农业生物技术研究中心 Application of TuMV-CP gene fragment-mediated RNAi carrier in cultivation of anti-TuMV transgenic plant
CN104531756A (en) * 2014-10-15 2015-04-22 湖南省烟草公司长沙市公司 Method for providing tobacco with potato Y virus resistance and VIGS carrier
CN107964551A (en) * 2017-12-07 2018-04-27 山东农业大学 The structure of watermelon mosaic virus infectious clone expression vector and application
CN111118216B (en) * 2020-01-18 2022-09-20 广西壮族自治区农业科学院 PCR primer group and kit for detecting sweet pepper vein mottle virus and application of PCR primer group and kit
CN116675751B (en) * 2023-06-08 2024-01-26 山东农业大学 Application of SWEET1g protein and encoding gene thereof in resisting potato viruses

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995012669A1 (en) * 1993-11-01 1995-05-11 The Texas A & M University System Expression of foreign genes using a replicating polyprotein producing virus vector

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995012669A1 (en) * 1993-11-01 1995-05-11 The Texas A & M University System Expression of foreign genes using a replicating polyprotein producing virus vector

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
BLANC, S., ET AL.: "A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus", VIROLOGY, vol. 231, 1997, pages 141 - 147, XP002118205 *
FUCHS, M., ET AL.: "Management of virus diseases by classical and engineered protection", MOLECULAR PLANT PATHOLOGY ON-LINE [HTTP://WWW.BSPP.ORG.UK/MPPOL/] 1997/0116FUCHS, 1997, pages 1 - 19, XP002118200, Retrieved from the Internet <URL:http://www.bspp.org.uk./mppol/1997/0116fuchs/> [retrieved on 19991008] *
GAL-ON, A., ET AL.: "A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on amplification", JOURNAL OF GENERAL VIROLOGY, vol. 73, 1992, pages 2183 - 2187, XP002118198 *
GAL-ON, A., ET AL.: "Particle bombardment drastically increases the infectivity of cloned DNA of zucchini yellow mosaic potyvirus", JOURNAL OF GENERAL VIROLOGY, vol. 76, 1995, pages 3223 - 3227, XP002118207 *
GRANIER, F., ET AL.: "Helper component - zucchini yellow mosaic virus (strain E15-PAT)", PIR ACCESSION NO:JQ2396, 30 September 1993 (1993-09-30), XP002118203 *
GRANIER, F., ET AL.: "Mutations in zucchini yellow mosaic virus helper component protein associated with loss of aphid transmissibility", JOURNAL OF GENERAL VIROLOGY, vol. 74, 1993, pages 2737 - 2742, XP002118202 *
HUET, H., ET AL.: "Zucchini yellow mosaic virus (ZYMV) HC-PRO gene", EMBL ACCESSION NO: X77756, 25 April 1994 (1994-04-25), XP002118197 *
HUET, H., ET AL: "Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility", JOURNAL OF GENERAL VIROLOGY, vol. 75, 1994, pages 1407 - 1414, XP002118196 *
LECOQ, H., ET AL.: "Characterization of a zucchini yellow mosaic virus isolate with a deficient helper component", PHYTOPATHOLOGY, vol. 81, 1991, pages 1087 - 1091, XP002118204 *
LECOQ, H., ET AL.: "Control of zucchini yellow mosaic virus in squash by cross protection", PLANT DISEASE, vol. 75, 1991, pages 208 - 211, XP002118201 *
LIVNEH, ORNA., ET AL.: "Plants transformed with the first (nonstructural) three cistrons of potato virus Y are resistant to potato virus infection", TRANSGENICS (1995), 1(5), 565-71, XP002118206 *
MAIA, I.G., ET AL.: "Potyviral HC-PRO: a multifunctional protein", JOURNAL OF GENERAL VIROLOGY, vol. 77, 1996, pages 1335 - 1341, XP002118199 *
PENG, Y.-H., ET AL.: "Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions", JOURNAL OF GENERAL VIROLOGY, vol. 79, April 1998 (1998-04-01), pages 897 - 904, XP002118215 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108823174B (en) * 2018-06-28 2021-07-20 山东农业大学 Development and Application of Attenuated Vaccine of Papaya Ringspot Virus Watermelon Strain

Also Published As

Publication number Publication date
JP2002536956A (en) 2002-11-05
AU3052599A (en) 1999-10-25
WO1999051749A2 (en) 1999-10-14
TR200002909T2 (en) 2001-02-21
EP1068330A2 (en) 2001-01-17
CN1303433A (en) 2001-07-11
CA2327445A1 (en) 1999-10-14

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