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WO1999051629A3 - New compounds for dna-transfection - Google Patents

New compounds for dna-transfection Download PDF

Info

Publication number
WO1999051629A3
WO1999051629A3 PCT/EP1999/002361 EP9902361W WO9951629A3 WO 1999051629 A3 WO1999051629 A3 WO 1999051629A3 EP 9902361 W EP9902361 W EP 9902361W WO 9951629 A3 WO9951629 A3 WO 9951629A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
dna
new compounds
chain
basic
Prior art date
Application number
PCT/EP1999/002361
Other languages
German (de)
French (fr)
Other versions
WO1999051629A2 (en
Inventor
Eric Argirios Kitas
Ernst-Juergen Schlaeger
Original Assignee
Roche Diagnostics Gmbh
Eric Argirios Kitas
Schlaeger Ernst Juergen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Eric Argirios Kitas, Schlaeger Ernst Juergen filed Critical Roche Diagnostics Gmbh
Priority to JP2000542350A priority Critical patent/JP2002510706A/en
Priority to EP99919208A priority patent/EP1068225A2/en
Priority to CA002327367A priority patent/CA2327367A1/en
Priority to AU37065/99A priority patent/AU3706599A/en
Publication of WO1999051629A2 publication Critical patent/WO1999051629A2/en
Publication of WO1999051629A3 publication Critical patent/WO1999051629A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)

Abstract

The invention relates to conjugates of lipids and basic, modified membrane disturbing peptides, characterized in that the peptides comprises a reversed amide backbone, particularly compounds of formula (I), wherein R?1 and R2¿ are a hydrocarbyl moiety of a straight-chain or branched-chain, saturated or unsaturated aliphatic carboxylic acid or a phospholipid moiety, R3 is a basic, modified membrane disturbing peptide with a reversed amide backbone, Y is C¿2-10? alkylene, X is -C(O)-NH- or -S-S-, and salts thereof.
PCT/EP1999/002361 1998-04-07 1999-04-07 New compounds for dna-transfection WO1999051629A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000542350A JP2002510706A (en) 1998-04-07 1999-04-07 Novel compounds for DNA transfection
EP99919208A EP1068225A2 (en) 1998-04-07 1999-04-07 New compounds for dna-transfection
CA002327367A CA2327367A1 (en) 1998-04-07 1999-04-07 New compounds for dna-transfection
AU37065/99A AU3706599A (en) 1998-04-07 1999-04-07 New compounds for dna-transfection

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP98106302 1998-04-07
EP98106302.7 1998-04-07
EP98124837 1998-12-30
EP98124837.0 1998-12-30

Publications (2)

Publication Number Publication Date
WO1999051629A2 WO1999051629A2 (en) 1999-10-14
WO1999051629A3 true WO1999051629A3 (en) 1999-12-23

Family

ID=26149151

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/002361 WO1999051629A2 (en) 1998-04-07 1999-04-07 New compounds for dna-transfection

Country Status (5)

Country Link
EP (1) EP1068225A2 (en)
JP (1) JP2002510706A (en)
AU (1) AU3706599A (en)
CA (1) CA2327367A1 (en)
WO (1) WO1999051629A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19925143A1 (en) * 1999-06-02 2000-12-07 Aventis Pharma Gmbh New liposomal vector complexes and their use for gene therapy
GB9915074D0 (en) * 1999-06-28 1999-08-25 Cortecs Plc Ligand-binding composition
WO2001051092A2 (en) * 2000-01-07 2001-07-19 University Of Washington Enhanced transport of agents using membrane disruptive agents
CN1313327A (en) * 2000-03-15 2001-09-19 上海博德基因开发有限公司 Polypeptide-human junctional adhesion molecule 12 and polynucleotide for coding it
CA2352645A1 (en) * 2000-07-28 2002-01-28 F. Hoffmann-La Roche Ag Compositions for dna transfection
GB0024428D0 (en) * 2000-10-05 2000-11-22 King S College Absorption enhancers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0784984A2 (en) * 1996-01-17 1997-07-23 F. Hoffmann-La Roche Ag Transfection competent molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0784984A2 (en) * 1996-01-17 1997-07-23 F. Hoffmann-La Roche Ag Transfection competent molecules

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; SCHLAEGER, E.-J. ET AL: "Synergistic enhancement of transient expression by dioleoyl -melittin (DOM) and polyethylenimine (PEI) in mammalian cells in suspension culture", XP002116436, retrieved from STN Database accession no. 130:61776 *
DEMPSEY C E: "The Actions of Mellitin on Membranes", BIOCHIMICA ET BIOPHYSICA ACTA. MR. REVIEWS ON BIOMEMBRANES, vol. 1031, 1 January 1990 (1990-01-01), pages 143 - 161, XP002088446, ISSN: 0304-4157 *
JUVVADI, PADMAJA ET AL: "Synthetic Melittin, Its Enantio, Retro, and Retroenantio Isomers, and Selected Chimeric Analogs: Their Antibacterial, Hemolytic, and Lipid Bilayer Action", J. AM. CHEM. SOC. (1996), 118(38), 8989-8997, 1996, XP002116435 *
LEGENDRE J Y ET AL: "Short-Chain Phospholipids Enhance Amphipatic Peptide-Mediated Gene Transfer", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 217, no. 1, 5 December 1995 (1995-12-05), pages 179 - 185, XP002088443, ISSN: 0006-291X *
LEGENDRE J Y: "Dioleoylmellitin as a Novel Serum- Insensitive Reagent for Efficient Transfection of Mammalian Cells", BIOCONJUGATE CHEMISTRY, vol. 8, 1 January 1997 (1997-01-01), pages 57 - 63, XP002088445, ISSN: 1043-1802 *
NEW DEV. NEW APPL. ANIM. CELL TECHNOL., PROC. ESACT MEET., 15TH (1998), MEETING DATE 1997, 117-120. EDITOR(S): MERTEN, OTTO-WILHELM;PERRIN, PIERRE; GRIFFITHS, BRYAN. PUBLISHER: KLUWER, DORDRECHT, NETH. *

Also Published As

Publication number Publication date
WO1999051629A2 (en) 1999-10-14
EP1068225A2 (en) 2001-01-17
AU3706599A (en) 1999-10-25
JP2002510706A (en) 2002-04-09
CA2327367A1 (en) 1999-10-14

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