+

WO1999043833A1 - Plantes transgeniques resistantes aux maladies - Google Patents

Plantes transgeniques resistantes aux maladies Download PDF

Info

Publication number
WO1999043833A1
WO1999043833A1 PCT/IB1998/000232 IB9800232W WO9943833A1 WO 1999043833 A1 WO1999043833 A1 WO 1999043833A1 IB 9800232 W IB9800232 W IB 9800232W WO 9943833 A1 WO9943833 A1 WO 9943833A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
pyruvate decarboxylase
plants
sequence
expression
Prior art date
Application number
PCT/IB1998/000232
Other languages
English (en)
Inventor
Cornelius Jan Kuhlemeier
Million Tadege
Isabelle Dupuis
Marcel Bucher
Original Assignee
Universität Bern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universität Bern filed Critical Universität Bern
Priority to AU60031/98A priority Critical patent/AU6003198A/en
Priority to PCT/IB1998/000232 priority patent/WO1999043833A1/fr
Publication of WO1999043833A1 publication Critical patent/WO1999043833A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Definitions

  • the present invention relates to plants and methods for their production, said plants being disease resistant due to transformation with pyruvate decarboxylase (PDC) encoding nucleic acid sequences.
  • PDC pyruvate decarboxylase
  • Preferred plants are those of the solanaceae family, and within said family preferred plants are potatoes.
  • the disease resistance comprises resistance to Phytoph tora infestans and resistance to the potato virus Y.
  • the plants' defense response to incompatible pathogen interactions is often manifested as rapid localized host cell death termed the hypersensitive response (HR) .
  • HR hypersensitive response
  • HR is thought to contribute to the containment of the pathogen and is associated with most but not all incompatible host-pathogen interactions and disease resistance (Dangl et al., 1996; Hammond-Kosack and Jones, 1996) .
  • a battery of defense reactions are initiated by the plant.
  • Purified bacterial elicitors can induce HR cell death and disease resistance response when applied locally to a plant " (He et al . , 1993) indicating that HR is a preset genetic program that can be activated by external factors .
  • the dominant Lesl and the recessive lethal leaf spot ( llsl ) mutations mimic stereotypic symp- toms of Helminthospori um maydi s and Helminthospori um car- bonum infections on susceptible maize, respectively (Neuffer and Calvert, 1975; Walbot et al . , 1983).
  • the goal of the present invention was to provide a method for stimulating defense response in plants leading to disease resistance, as well as disease resistant plants.
  • pyruvate decarboxylase for example such of bacterial origin, like the one described by Conway T. et al . (1987), stimulates the defense response of plants transformed with the respective gene.
  • one subject of the present invention is a method for stimulating the defense response of plants and plant cells leading to disease resistance, by in- creasing the average pyruvate decarboxylase level.
  • Another subject of the present invention is to provide a method for producing a plant or plant cell or reproduction material of said plant comprising a pyruvate decarboxylase encoding DNA sequence incorporated in the genome of said plant, plant cell or reproduction material in a non-natural environment allowing the expression of said pyruvate decarboxylase.
  • transgenic as it is used herein does not only refer to plants or plant cells or reproduction material or pyruvate decarboxylase production comprising or induced by a heterologous gene, but also by a homologous gene in a non-natural environment. However, due to possible down-regulation rather than overexpression of the target gene in the case of homologous genes, heterologous genes are preferred.) It was found that the defense response was induced in the transgenic plants, even in the absence of visible lesions.
  • transgenic plants even those with lit- tie or no visible lesion development showed markedly enhanced resistance against pathogen infection, as for example infection with bacteria such as Erwinia, Pseudo- monas; fungi such as Fusari um, Al ternaria, Phyti um, Phytophtora and in particular Phytoph tora infestans; viruses such as Potexvirus, e.g. Potato Virus X and Potyvirus, in particular Potato Virus Y; mycoplasmas; nematodes; insects. For example a 10 to 300 fold decrease in the number of Phytophtora infestans sporangia was found compared to the wild-type. Also an infection with viruses such as the potato virus Y (PVYO803) showed a resistance of transgenic plants.
  • bacteria such as Erwinia, Pseudo- monas
  • fungi such as Fusari um, Al ternaria, Phyti um, Phytophtora and in particular
  • the pyruvate decarboxylase gene Due to the fact that the transgenic plants comprising the pyruvate decarboxylase gene have a more or less strong tendency to form lesions, it is preferred that the pyruvate decarboxylase gene is present in a form providing minimal pyruvate decarboxylase related lesions with maximum pathogen resistance under the desired or most likely environmental conditions.
  • the transgenics usually produce higher (generally about 5 to 12-fold higher) levels of acetaldehyde and they usually export a higher (generally about 2 to 10-fold higher) level of sucrose from the leaves compared to the wild- type.
  • Starch content on the contrary, can be drastically reduced in the high level PDC expressing plants such as potatoes.
  • Analysis of physiological and molecular markers of hypersensitive cell death revealed that defense reactions similar to the HR were initiated in the potato transgenic plants. These included: deposition of callose in the leaf tissue, high level induction of pathogenesis- related (PR) protein encoding transcripts, and heightened resistance to pathogens such as fungus or viruses, e.g. P. infestans and potato virus Y.
  • PR pathogenesis-related
  • the present invention is not limited to a specific pyruvate decarboxylase, e.g. of Zymomonas mobi - lis nor to specific plants, although potatoes - because of their great relevance as nutrient - are preferred plants.
  • any pyruvate decarboxylase encoding DNA sequence with the ability to enhance the naturally present pyruvate decar- boxylase level (without killing the plant) is suitable.
  • Suitable DNA sequences are e.g. all sequences encoding a pyruvate decarboxylase or a fragment or mutation thereof 6
  • DNA sequences can be naturally occurring genomic sequences or cDNA sequences, sequences that are identical with such sequences but for the degeneracy of the genetic code as well as sequences encoding fragments and mutations provided that they stimulate defense response.
  • Said encoding sequences can either be introduced into a suitable environment of the genome of the plant to be transformed ena- bling the expression of pyruvate decarboxylase or they can be introduced into the genome of the plant together with their natural or adapted regulatory sequences.
  • the nucleotide sequence can be introduced into the plant, plant cells, parts of plants such as tissue or reproduc- tion material by any transformation method leading to incorporation of the nucleic acid sequence into the genome of the plant, plant cells, plant tissue or reproduction material.
  • Reproduction materials include stems, tubers, leafs, and cells, with tubers being preferred for pota- toes, but also calli.
  • a first interpretation of the results obtained might be that the response is triggered by an interaction of acetaldehyde with a component of the cell death machinery.
  • the physical and chemical properties of acetaldehyde will determine its interaction with endogenous molecules.
  • potatoes incubated under anoxia to enhance pyruvate decarboxylase activity, and exposed back to air produced high acetaldehyde levels, but no lesions could be observed.
  • an enhanced pyruvate decarboxylase activity might lead to lesion formation and pathogen resistance.
  • transgenic plants were measured, it was found that acetaldehyde accumulated to higher levels in the transgen- ics grown at 18 °C compared to the wild-type, and that L-8 produced as much acetaldehyde in the 25°C as any other of the transgenic potatoes at the 18°C growth condition.
  • the transgenic potatoes showed lesions at 18 °C, whereas L-8 at 25°C was virtually unaffected.
  • non-transformed potatoes incubated under anoxia and exposed back to air it was found that they produced high levels of acetaldehyde equivalent to L-8 under normoxia at 18 °C, but no lesions were observed.
  • another or other factors could also be relevant for the lesion formation and the defense response.
  • an increased amount of pyruvate decarboxylase in the cell in comparison with the wild type over a sufficient long time to activate defense response is likely to be the main factor.
  • any other method to increase said amount is also within the scope of the present invention, i.e. the application of pyruvate 'decarboxylase in any kind of carrier enabling the introduction of PDC into plant cells.
  • the lesion formation is largely reduced to ensure a good harvest with at the same time enhanced pathogen resistance.
  • This can e.g. be obtained by selection of the minimal lesion forming plants showing resistance or by variation of the promoter, e.g. by selecting a promoter that needs specific activation by a specific inducer.
  • Figure 1 shows the Zymomonas PDC expression and in vi tro enzymatic activity
  • Figure 1A representing the Zymomonas PDC protein expression in potato leaves, whereby twenty micro- grams of total soluble protein were loaded per lane and probed with anti- Zymomonas PDC antibody
  • Figure IB representing the PDC enzymatic activity, whereby the PDC activity was measured in the leaf 9
  • Figure 2 shows the acetaldehyde concentration in potato leaf tissue
  • Figure 2A showing chromatograms of control experiments, with the peaks representing: blk, water blank; con, control in which the wild-type leaf extract was incubated at 37 °C for 1 h; wt, wild-type leaf untreated; anx, wild-type leaf incubated under anoxia for 2 h and 10 in air; std, acetaldehyde standard (l ⁇ M), and
  • Figure 2B representing the acetaldehyde from plant leaves grown at 18 ⁇ 3°C
  • Figure 2C representing the acetaldehyde from plant leaves grown at 25+2 °C, whereby the acetaldehyde was measured as a fluorescent adduct with 1, 3-cyclohex- anedione in a perchloric acid extract of 4 week old leaves by HPLC, and whereby the values represent the mean + SE of 6 independent measurements.
  • Figure 3 shows the accumulation of PR gene transcripts in transgenic potato leaves, whereby total RNA (10 ⁇ g) from healthy (-lesion) or lesioned (+lesion) leaves of line 25 was loaded per lane and probed with specific cDNA probes indicated on the left side.
  • Figure 4 shows soluble sugars and starch in potato leaves, whereby the values represent the mean ⁇ SE of 5 measurements of individual plants, and whereby
  • Figure 4A represents the concentration of starch and soluble sugars in the leaf tissue from plants grown at 18 ⁇ 3°C
  • Figure 4B represents the concentration of soluble sugars in the petiole exudates of plants grown at 18 ⁇ 3°C
  • Figure 4C represents the concentration of soluble sugars in the petiole exudates of plants grown at 25+2 °C.
  • Figure 5 shows resistance tests to the fungus
  • Phytophtora infestans particularly the sporulation efficiency 6 days post-infection, whereby the values represent the mean ⁇ SE of 3 leaves of 8 individual plants for each line.
  • Figure 6 shows resistance tests to the PVY, particularly the virus titer measured by ELISA in three different wt and transgenic (L-17) plants.
  • the PDC gene from the obligate anaerobe Zymomonas mobilis was in- serted between the alfalfa mosaic virus (7 ⁇ MV) transla- tional enhancer and the nos terminator under the control of the 35S promoter (Odell J.T. et al . , (1985)) as de ⁇ scribed in Bucher et al . , (1994).
  • Transgene expression was variable and 4 lines accumulating the PDC protein to different levels (Figure 1A) were maintained in tissue culture by clonal propagation.
  • the in vi tro PDC enzymatic activity correlated with the level of protein accumulation, and showed more than a 6-fold increase in the case of highest expression in line 8 (L-8) as compared to the wild-type (wt) ( Figure IB) .
  • the transgenic plants displayed a lesion mimic phenotype, the severity of which correlated with the expression level of the ZymPOC protein, the highest expressors showing the most extensive lesions. Lesions began to develop about 4 weeks after planting in soil. In most cases, the lesion started at the tip of source leaves near the midrib as small brownish spots, and spread in all directions, primarily along the midrib and the veins. Within 3 to 5 days of the start of lesion formation, the lesion encompassed most of the leaf area leading to a dry, grey to brownish, and shrunken appearance. Finally, the whole leaf collapsed and abscised. The lesion continued to the next fully expanded source leaf, and proceeded to the one above even before the complete collapse of the one below it.
  • the timing and progression of the lesion showed dependence on the level of the transgene expression.
  • the lines that expressed the PDC protein at highest levels were the first to show the lesion appearance.
  • the difference in symptom appearance between the highest expressor (L-8) and the least expressors (L-21 and L-17) was at least 6-10 days.
  • the progression of the lesion was also dependent on the PDC protein levels.
  • L-8 and L-25 the lesion spread was fast and uncontrolled, leading to the death of the entire plant within 2-3 weeks after the onset of lesion formation.
  • L-17 and L-21 the lesions were localized, spread slowly to consume the entire leaf, but remained restricted to only a few source leaves, and never reached the top of the plant.
  • the transgenic plants were not reduced in height before lesion formation, and L-17 and L-21 grew to 12
  • Example 3 Acetaldehyde level in leaf tissue of transgenic potatoes
  • PDC is known to catalyze the first step in ethanolic fermentation, a decarboxylation of pyruvate yielding acetaldehyde and C0 2 .
  • ethanolic fermentation occurs only during oxygen limitation and some other stresses.
  • An attempt for constitutive high level expression of bacterial PDC in tobacco leaves did neither lead to measurable acetaldehyde production nor to visible lesion formation in the presence of oxygen as de- 13
  • Healthy leaf discs (2 per plant) were snap frozen in liquid nitrogen from leaf number 3 and 4 as counted from the top of a 4 week old potato.
  • the leaves were extracted with 6% perchloric acid at 4°C and incubated on ice for 2 h. After incubation, the samples were spun at maximum microfuge speed for 10 min at 4°C.
  • the supernatant was neutralized to pH 6.0-6.5 with 5M K 2 C0 3 on ice.
  • the neutralized extract was spun again for 10 min as above, and the supernatant was transferred to a precooled Eppendorf and kept on ice until derivatization.
  • Acetaldehyde in this extract was measured as a fluorescent adduct formed by a reaction with 1, 3-cyclohexanedione (CHD) essentially according to Helander et al . (1993).
  • the reaction mixture contained 150 ⁇ l ammonium acetate (20%,w/v, in water), 150 ⁇ l thiourea (6%, w/v, in water), 50 ⁇ l CHD 1.25%, w/v, in water), and 150 ⁇ l extract added to a 2 ml glass bottle in this order. Each bottle was immediately sealed after adding the extract, and all samples were incubated at 60°C in a gently shaking water both for 1 h. The samples were cooled on ice and 20 ⁇ l aliquots were analyzed by high- performance liquid chromatography (HPLC) .
  • HPLC high- performance liquid chromatography
  • Rheodyne RH 7010 injector with a 100 ⁇ l sample loop (Beckmann, Nyon, Switzerland)
  • Nucleosil 100-5 C18 reversed-phase analytical " column 40 x 250 mm i.d., 5 ⁇ m particle size; Macherey-Nagel, Oensingen, Switzerland
  • the column was eluted isocratically at a flow rate of 1.0 ml/min at ambient temperature with a mobile phase consisting of methanol-water (40: 60, v/v) .
  • a Kon- tron Model SFM-25 fluorescent detector (Kontron, Zurich, Switzerland) was used with excitation and emission wave- lengths of 366 and 440 nm, respectively.
  • This method allowed the determination of low levels of acetaldehyde in actively respiring normoxic po- tato leaves (see Figure 2) .
  • Figure 2A shows chromatograms of control experiments used to authenticate the assay conditions.
  • a small background peak appeared which has the same retention time as the acetaldehyde adduct.
  • a similar background peak was observed by Helander et al . (1993) .
  • wild-type potato leaves were incubated under anoxia, in which more than a 10-fold increase in acetaldehyde peak area was observed compared to the untreated wild-type ( Figure 2A) .
  • Figures 2B and C shows chromatograms of control experiments used to authenticate the assay conditions.
  • L-21 at 18°C and L-8 at 25°C have comparable acetaldehyde levels, yet L-21 developed severe symptoms at 18°C, whereas L-8 at 25°C was virtually unaf- fected. This clearly indicates that in addition to acetaldehyde concentration other physiological and environmental factors must contribute to lesion formation.
  • Example 4 Correlation between lesion formation and induction of the plant defense response
  • Callose deposition represents one of the earliest plant defense responses (Bradley et al., 1992), al- though it is not an exclusive marker of HR.
  • Callose deposition was detected as follows: Leaf discs for callose examination were bleached in a series of 50, 75 and 96% ethanol overnight. The cleared leaves were rinsed in water and stained for 1 h at room temperature in a humid chamber in a 0.05% (w/v) solution of aniline blue in 0.15 M K 2 HP0 4 . Stained leaves were examined under UV-light using excitation filter, 365 nm; dichromic mirror, 396 nm; and barrier filter, 420 nm. After treatment with aniline blue fluores- cence could be observed in transgenic leaves. 16
  • Leaf discs (2 per plant) were collected in liquid nitrogen from fully expanded source leaves after 8h of the light period. Most of the L-8 and L-25 plants growing at the 18 °C showed lesions at the time of sam- pling (4-5 weeks after planting to soil), but samples were collected from healthy looking leaves. Leaves were homogenised in an Eppendorf tube with 80% ethanol (v/v) and extracted for 90 min at 70 °C. Samples were spun at 13 000 rpm for 10 min, and the supernatant was stored at - 20°C for soluble sugars (sucrose, glucose, and fructose) determination. The pellet was washed with 1 ml of 17
  • sucrose translocation is a component of the developmentally regulated cell death initiation and exe- cution process.
  • Example 6 Phytophtora infestans resistance of transgenic potatoes
  • the response of the PDC transgenics to a virulent fungal inoculation was examined to determine whether the transgenics exhibit disease resistance.
  • Transgenic and wild-type potato leaves were infected with the fungal pathogen Phytoph tora infestans , causative agent of late blight disease, to which the wild-type Desiree variety is susceptible. Both the trans- genie and wild-type leaves were completely healthy at the time of infection.
  • An inoculum of Phytophtora infestans strain 94-18 was prepared by adding 15 ml of ice cold 0.5% glucose to a 3 weeks old culture on rye A medium (Ribeiro, 1978) . After 3 h incubation at 4°C to allow for the release of the zoospores, spores were counted and the concentration was adjusted to 25,000 per ml. Eight plants per line were grown in the greenhouse for 3 weeks at 24 +_ 2°C and transferred to 17°C for the infection test. On each plant, 3 leaves were infected with 4 droplets of 5 ⁇ l of spore suspension per leaf. Mock inoculations were 19
  • Example 7 Resistance test of wild type and transgenic PDC potato plants (line 17) to infection by potato virus Y (PVY O803) .
  • Wt plants showed symptoms of viral infection in the infected leaf but also showed PVY symptoms in the upper leaves of the plant.
  • the transgenic plants showed symptoms in the inoculated leaf, but no le- sions developed in the rest of the plant.
  • the inoculated leaf was found to contain viruses in both the wt and the transgenic plants 13 dpi. In the wt plants, the titer was high in the upper leaf 17 and 20 dpi. However the virus could not be detected in the upper leaves in the transgenic plants (Fig. 6) .
  • transgenic plants showed a resistance to potato virus Y, both at the macroscopic level, with absence of any visible lesions, and at the ELISA analysis level, where the virus can not be detected in the upper leaf analyzed. While there are shown and described presently preferred embodiments of the invention, it is to be distinctly understood that the invention is not limited 21
  • Pseudomonas syringae pv. syringae harpins Pss A protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. CeJJ 73, 1255-1266.
  • Salicylic acid-independent induction of pathogenesis-related protein transcripts by sugars is dependent on leaf developmental age.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Méthode de stimulation de la réaction de défense et de la résistance aux maladies de plantes, notamment de pommes de terre, par augmentation du niveau de pyruvate décarboxylase intracellulaire, et méthode de production de plantes capables d'exprimer un niveau accru de pyruvate décarboxylase et plantes respectives etc.
PCT/IB1998/000232 1998-02-26 1998-02-26 Plantes transgeniques resistantes aux maladies WO1999043833A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU60031/98A AU6003198A (en) 1998-02-26 1998-02-26 Disease resistant transgenic plants
PCT/IB1998/000232 WO1999043833A1 (fr) 1998-02-26 1998-02-26 Plantes transgeniques resistantes aux maladies

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB1998/000232 WO1999043833A1 (fr) 1998-02-26 1998-02-26 Plantes transgeniques resistantes aux maladies

Publications (1)

Publication Number Publication Date
WO1999043833A1 true WO1999043833A1 (fr) 1999-09-02

Family

ID=11004689

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1998/000232 WO1999043833A1 (fr) 1998-02-26 1998-02-26 Plantes transgeniques resistantes aux maladies

Country Status (2)

Country Link
AU (1) AU6003198A (fr)
WO (1) WO1999043833A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005003362A3 (fr) * 2003-03-10 2005-04-21 Athenix Corp Methodes destinees a conferer une resistance aux herbicides
US7807881B2 (en) 2003-03-10 2010-10-05 Athenix Corp. Methods to confer herbicide resistance
CN118562777A (zh) * 2024-08-05 2024-08-30 中国热带农业科学院三亚研究院 一种具有抗病功能的ScPDC1蛋白及其编码基因和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002193A1 (fr) * 1988-08-31 1990-03-08 University Of Florida Production d'ethanol par des souches d'escherichia coli mises au point genetiquement
WO1997027295A1 (fr) * 1996-01-23 1997-07-31 Horticulture Research International Genes associes au murissement des fruits

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990002193A1 (fr) * 1988-08-31 1990-03-08 University Of Florida Production d'ethanol par des souches d'escherichia coli mises au point genetiquement
WO1997027295A1 (fr) * 1996-01-23 1997-07-31 Horticulture Research International Genes associes au murissement des fruits

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BUCHER M ET AL: "Ethanolic fermentation in transgenic tobacco expressing *Zymomonas* *mobilis* *pyruvate* *decarboxylase*.", EMBO J, JUN 15 1994, 13 (12) P2755-63, ENGLAND, XP002071433 *
CONWAY, T. ET AL.: "Promoter and nucleotide sequence of the Zymomonas mobilis pyruvate decarboxylase", J. OF BACTERIOLOGY, vol. 169, 1987, pages 949 - 954, XP002071434 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005003362A3 (fr) * 2003-03-10 2005-04-21 Athenix Corp Methodes destinees a conferer une resistance aux herbicides
US7807881B2 (en) 2003-03-10 2010-10-05 Athenix Corp. Methods to confer herbicide resistance
CN118562777A (zh) * 2024-08-05 2024-08-30 中国热带农业科学院三亚研究院 一种具有抗病功能的ScPDC1蛋白及其编码基因和应用
CN118562777B (zh) * 2024-08-05 2024-11-01 中国热带农业科学院三亚研究院 一种具有抗病功能的ScPDC1蛋白及其编码基因和应用

Also Published As

Publication number Publication date
AU6003198A (en) 1999-09-15

Similar Documents

Publication Publication Date Title
Tadege et al. Activation of plant defense responses and sugar efflux by expression of pyruvate decarboxylase in potato leaves
Herbers et al. Systemic acquired resistance mediated by the ectopic expression of invertase: possible hexose sensing in the secretory pathway.
Hunt et al. Systemic acquired resistance signal transduction
Li et al. Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance
Xia et al. An extracellular aspartic protease functions in Arabidopsis disease resistance signaling
Dewdney et al. Three unique mutants of Arabidopsis identify eds loci required for limiting growth of a biotrophic fungal pathogen
Johnson et al. Wound-inducible potato inhibitor II genes: enhancement of expression by sucrose
Wu et al. Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants.
Beffa et al. Cholera toxin elevates pathogen resistance and induces pathogenesis‐related gene expression in tobacco.
LeBrasseur et al. Local and systemic wound‐induction of RNase and nuclease activities in Arabidopsis: RNS1 as a marker for a JA‐independent systemic signaling pathway
Karban et al. Induced resistance against pathogens and herbivores: an overview
Helliwell et al. Ethylene biosynthesis and signaling is required for rice immune response and basal resistance against Magnaporthe oryzae infection
Bienert et al. A pleiotropic drug resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta
Gandía et al. Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus
Sun et al. A novel Medicago truncatula calmodulin‐like protein (MtCML42) regulates cold tolerance and flowering time
US8163979B2 (en) Antifungal plant proteins and methods of their use
PL178652B1 (pl) Zrekombinowana cząsteczka dwuniciowego DNA, sposób wytwarzania genetycznie stransformowanych, odpornych na choroby roślin, oraz genetycznie stransformowana komórka roślinna ziemniaka lub pszenicy
Manoharan et al. Expression of 3-OH trichothecene acetyltransferase in barley (Hordeum vulgare L.) and effects on deoxynivalenol
O'mahony et al. The involvement of ubiquitin in vegetative desiccation tolerance
Simmons et al. The maize lethal leaf spot 1 mutant has elevated resistance to fungal infection at the leaf epidermis
CA2298882A1 (fr) Plantes transgeniques employant le gene de tdc (tryptophane decarboxylase) en vue d'une amelioration des cultures
WO1999043833A1 (fr) Plantes transgeniques resistantes aux maladies
EP1018553A1 (fr) Plantes transgéniques avec les gènes divergents SCaM4 et SCaM5 pour obtenir une résistance aux maladies multiples
Bolingue et al. The MtSNF4b subunit of the sucrose non‐fermenting‐related kinase complex connects after‐ripening and constitutive defense responses in seeds of Medicago truncatula
WO2014028426A1 (fr) Compositions et procédés d'amélioration de la résistance des plantes contre les parasites

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
NENP Non-entry into the national phase

Ref country code: KR

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载