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WO1999043787A3 - A living chimeric skin replacement - Google Patents

A living chimeric skin replacement Download PDF

Info

Publication number
WO1999043787A3
WO1999043787A3 PCT/US1999/003859 US9903859W WO9943787A3 WO 1999043787 A3 WO1999043787 A3 WO 1999043787A3 US 9903859 W US9903859 W US 9903859W WO 9943787 A3 WO9943787 A3 WO 9943787A3
Authority
WO
WIPO (PCT)
Prior art keywords
epithelial cells
dermal
skin
replacement
component
Prior art date
Application number
PCT/US1999/003859
Other languages
French (fr)
Other versions
WO1999043787A2 (en
Inventor
Gail K Naughton
R Emmett Pinney
Jonathan N Mansbridge
Original Assignee
Advanced Tissue Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Tissue Sciences Inc filed Critical Advanced Tissue Sciences Inc
Priority to EP99936092A priority Critical patent/EP1062322A2/en
Priority to AU33077/99A priority patent/AU760470B2/en
Priority to KR1020007009299A priority patent/KR20010072553A/en
Priority to JP2000533527A priority patent/JP2002504412A/en
Priority to CA002324452A priority patent/CA2324452A1/en
Priority to NZ506510A priority patent/NZ506510A/en
Publication of WO1999043787A2 publication Critical patent/WO1999043787A2/en
Publication of WO1999043787A3 publication Critical patent/WO1999043787A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention comprises skin replacements for improved wound healing. In one embodiment, the skin replacement is chimeric and comprises harvesting autologous epithelial cells from a patient and seeding them onto a biocompatible substrate containing allogeneic epithelial cells cultured in vitro. The living chimeric skin replacement may be implanted over a permanent dermal replacement previously implanted at the wound site. In another embodiment the skin replacement is a composite comprising an inner, middle and outer component, comprising (a) an inner component comprising a biocompatible dermal construct having a biodegradable or removable scaffold as a base; (b) a middle component comprising epithelial cells; and (c) an outer component comprising epithelial cells cultured in vitro on a dermal construct comprising a dermal portion having a biodegradable or removable scaffold as a base, said dermal portion being combined with a transitional covering and facing inward toward said middle component of epithelial cells. The invention further comprises methods of making and implanting the various skin replacements of the invention.
PCT/US1999/003859 1998-02-24 1999-02-23 A living chimeric skin replacement WO1999043787A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP99936092A EP1062322A2 (en) 1998-02-24 1999-02-23 A living chimeric skin replacement
AU33077/99A AU760470B2 (en) 1998-02-24 1999-02-23 A living chimeric skin replacement
KR1020007009299A KR20010072553A (en) 1998-02-24 1999-02-23 A Living Chimeric Skin Replacement
JP2000533527A JP2002504412A (en) 1998-02-24 1999-02-23 Live chimera skin replacement
CA002324452A CA2324452A1 (en) 1998-02-24 1999-02-23 A living chimeric skin replacement
NZ506510A NZ506510A (en) 1998-02-24 1999-02-23 A permanent skin replacement comprising allogenic epithelial cells cultured in vitro and autologous epithelial cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7570498P 1998-02-24 1998-02-24
US60/075,704 1998-02-24

Publications (2)

Publication Number Publication Date
WO1999043787A2 WO1999043787A2 (en) 1999-09-02
WO1999043787A3 true WO1999043787A3 (en) 1999-11-25

Family

ID=22127475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/003859 WO1999043787A2 (en) 1998-02-24 1999-02-23 A living chimeric skin replacement

Country Status (7)

Country Link
EP (1) EP1062322A2 (en)
JP (1) JP2002504412A (en)
KR (1) KR20010072553A (en)
AU (1) AU760470B2 (en)
CA (1) CA2324452A1 (en)
NZ (1) NZ506510A (en)
WO (1) WO1999043787A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9511094B2 (en) 2001-12-07 2016-12-06 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of stroke and related diseases and disorders

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
DE19949290A1 (en) * 1999-10-12 2001-04-26 Albrecht Bettermann Particulate construct for use in transplantation medicine
DE10109136A1 (en) * 2001-02-26 2002-09-12 Cytotools Gmbh Using inhibitor of thrombospondin-1 interaction for promoting wound healing, also for treating living skin replacements, inhibits apoptosis of wound-repair cells
EP1390754B1 (en) * 2001-04-24 2007-08-01 Wisconsin Alumni Research Foundation Method and composition for skin grafts
US20050095228A1 (en) 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
US7771716B2 (en) 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US7741116B2 (en) 2002-03-06 2010-06-22 University Of Cincinnati Surgical device for skin therapy or testing
GB0305976D0 (en) * 2003-03-14 2003-04-23 Wild Andrew M Surgical clip
JP5219030B2 (en) * 2005-11-24 2013-06-26 満 明石 Stimulus-responsive degradation gel
WO2009067601A1 (en) * 2007-11-20 2009-05-28 Pioneer Surgical Orthobiologics, Inc. Cryopreservation of cells using cross-linked bioactive hydrogel matrix particles
WO2010021993A1 (en) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
CA2963468A1 (en) * 2014-10-03 2016-04-07 Cytori Therapeutics, Inc. Use of regenerative cells in mitigating burn progression and improving skin graft incorporation and healing
JP7258298B2 (en) * 2015-10-29 2023-04-17 エルジー ハウスホールド アンド ヘルスケア リミテッド Novel occlusive composition

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460939A (en) * 1986-04-18 1995-10-24 Advanced Tissue Sciences, Inc. Temporary living skin replacement
WO1997008295A1 (en) * 1995-08-25 1997-03-06 Lifecell Corporation Reconstituted skin
US5610007A (en) * 1993-01-21 1997-03-11 Universite Laval Chimeric sheets of epithelial cells
US5693332A (en) * 1995-08-11 1997-12-02 The Regents Of The University Of California Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460939A (en) * 1986-04-18 1995-10-24 Advanced Tissue Sciences, Inc. Temporary living skin replacement
US5610007A (en) * 1993-01-21 1997-03-11 Universite Laval Chimeric sheets of epithelial cells
US5693332A (en) * 1995-08-11 1997-12-02 The Regents Of The University Of California Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure
US5693332C1 (en) * 1995-08-11 2001-01-09 Univ California Human keratinocytes supported on a hydrophilic membrane and methods of using same to effect wound closure
WO1997008295A1 (en) * 1995-08-25 1997-03-06 Lifecell Corporation Reconstituted skin

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ROUABHIA, MAHMOUD: "Skin replacement using chimeric ( allogeneic -syngeneic) cultured epidermal sheets.", M-S (MEDECINE SCIENCES), (1996) VOL. 12, NO. 12, PP. 1370-1377., XP002116665 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9511094B2 (en) 2001-12-07 2016-12-06 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of stroke and related diseases and disorders

Also Published As

Publication number Publication date
AU3307799A (en) 1999-09-15
JP2002504412A (en) 2002-02-12
KR20010072553A (en) 2001-07-31
NZ506510A (en) 2003-03-28
EP1062322A2 (en) 2000-12-27
WO1999043787A2 (en) 1999-09-02
AU760470B2 (en) 2003-05-15
CA2324452A1 (en) 1999-09-02

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