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WO1999041614A3 - Method for detecting intimin producing microorganisms - Google Patents

Method for detecting intimin producing microorganisms Download PDF

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Publication number
WO1999041614A3
WO1999041614A3 PCT/GB1999/000467 GB9900467W WO9941614A3 WO 1999041614 A3 WO1999041614 A3 WO 1999041614A3 GB 9900467 W GB9900467 W GB 9900467W WO 9941614 A3 WO9941614 A3 WO 9941614A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
intimin
detecting
producing microorganisms
well
Prior art date
Application number
PCT/GB1999/000467
Other languages
French (fr)
Other versions
WO1999041614A2 (en
Inventor
Miranda Batchelor
Gordon Dougan
Gad Frankel
Original Assignee
Imperial College
Miranda Batchelor
Gordon Dougan
Gad Frankel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial College, Miranda Batchelor, Gordon Dougan, Gad Frankel filed Critical Imperial College
Priority to EP99905052A priority Critical patent/EP1057035A2/en
Priority to CA002320753A priority patent/CA2320753A1/en
Priority to AU25356/99A priority patent/AU2535699A/en
Publication of WO1999041614A2 publication Critical patent/WO1999041614A2/en
Publication of WO1999041614A3 publication Critical patent/WO1999041614A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/1228—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K16/1232—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia from Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
    • C07K16/1228—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G01N2333/425—Zeins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods of detecting and/or diagnosing intimin-expressing microorganisms such as enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli, C. rodentium and/or RDEC-1, as well as kits for use in such methods are provided. In particular, methods based antisera raised against conserved regions are described, as well as polypeptide regions useful in the production of antisera. Vaccines based on such peptides are also described herein. In addition, methods of typing/classification of such bacteria are also described herein. Finally, methods for the isolation of intimin-expressing microorganisms are also provided.
PCT/GB1999/000467 1998-02-16 1999-02-16 Method for detecting intimin producing microorganisms WO1999041614A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99905052A EP1057035A2 (en) 1998-02-16 1999-02-16 Method for detecting intimin producing microorganisms
CA002320753A CA2320753A1 (en) 1998-02-16 1999-02-16 Method for detecting intimin producing microorganisms
AU25356/99A AU2535699A (en) 1998-02-16 1999-02-16 Method for detecting intimin producing microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9803322.8 1998-02-16
GBGB9803322.8A GB9803322D0 (en) 1998-02-16 1998-02-16 Methods

Publications (2)

Publication Number Publication Date
WO1999041614A2 WO1999041614A2 (en) 1999-08-19
WO1999041614A3 true WO1999041614A3 (en) 1999-10-07

Family

ID=10827122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/000467 WO1999041614A2 (en) 1998-02-16 1999-02-16 Method for detecting intimin producing microorganisms

Country Status (5)

Country Link
EP (1) EP1057035A2 (en)
AU (1) AU2535699A (en)
CA (1) CA2320753A1 (en)
GB (1) GB9803322D0 (en)
WO (1) WO1999041614A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9908525D0 (en) * 1999-04-14 1999-06-09 Imperial College Methods
CA2339436A1 (en) 2001-03-15 2002-09-15 Josee Harel Production of antibodies against virulence factors associated with strains of escherichia coli (aeec), and their use
AU2002251202A1 (en) * 2001-03-29 2002-10-15 Imperial College Innovations Limited Intimins for the prevention or treatment of infections: i
WO2003052143A2 (en) * 2001-12-19 2003-06-26 Angles D Auriac Marc B New primers for the detection and identification of bacterial indicator groups and virulence factors
CN1253571C (en) * 2004-03-12 2006-04-26 天津生物芯片技术有限责任公司 Nucleotide peculiar to 0-antigen of 0114 type bacillus coil

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2078716A1 (en) * 1992-09-21 1994-03-22 Joyce De Azavedo Attaching and effacing protein of enterohemorrhagic e. coli
WO1997040161A1 (en) * 1996-04-19 1997-10-30 Henry M. Jackson Foundation For The Advancement Of Military Medecine Histidine-tagged intimin and methods of using intimin to stimulate an immune response and as an antigen carrier with targeting capability
WO1997040177A1 (en) * 1996-04-19 1997-10-30 Henry M. Jackson Foundation For The Advancement Of Military Medicine Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens
US5747293A (en) * 1995-03-23 1998-05-05 Imperial College Of Science, Technology & Medicine Intimin-like proteins of E. coli

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2078716A1 (en) * 1992-09-21 1994-03-22 Joyce De Azavedo Attaching and effacing protein of enterohemorrhagic e. coli
US5747293A (en) * 1995-03-23 1998-05-05 Imperial College Of Science, Technology & Medicine Intimin-like proteins of E. coli
WO1997040161A1 (en) * 1996-04-19 1997-10-30 Henry M. Jackson Foundation For The Advancement Of Military Medecine Histidine-tagged intimin and methods of using intimin to stimulate an immune response and as an antigen carrier with targeting capability
WO1997040177A1 (en) * 1996-04-19 1997-10-30 Henry M. Jackson Foundation For The Advancement Of Military Medicine Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
A.E. JERSE ET AL.: "A genetic locus of enteropathogenic Escherchia coli necessary for the production of attaching and effacing lesions on tissue culture cells.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 87, October 1990 (1990-10-01), pages 7839 - 7843 *
DATABASE SWISSPROT 1 January 1900 (1900-01-01), XP002111948, Database accession no. P19809 *
DATABASE SWISSPROT 1 January 1900 (1900-01-01), XP002111950, Database accession no. I41193 *
DATABASE TREMBL PROTEIN SEQUE 1 January 1900 (1900-01-01), XP002111949, Database accession no. P77075 *
FRANKEL G ET AL: "CHARACTERIZATION OF THE C-TERMINAL DOMAINS OF INTIMIN-LIKE PROTEINSOF ENTEROPATHOGENIC AND ENTROHEMORRHAGIC ESCHERICHIA COLI, CITROBACTER FREUNDII, AND HAFNIA ALVEI", INFECTION AND IMMUNITY, vol. 62, no. 5, 1 May 1994 (1994-05-01), pages 1835 - 1842, XP002040895 *
FRANKEL G ET AL: "MOLECULAR CHARACTERIZATION OF A CARBOXY-TERMINAL EUKARYOTIC-CELL BINDING DOMAIN OF INTIMIN FROM ENTEROPATHOGENIC ESCHERICHIA COLI", INFECTION AND IMMUNITY, vol. 63, no. 11, 1 November 1995 (1995-11-01), pages 4323 - 4328, XP002040896 *
G. BEEBAKHEE, ET AL.: "Cloning and nucleotide sequence of the eae gene homolohue from enterohemorrhagic Escherichia coli serotype O157:H7", FEMS MICROBIOLOGY LETTERS, vol. 91, 1992, pages 63-68, XP002040899 *
J. ADU-BOBIE ET AL.: "Detection of intimins alpha, beta, gamma and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens.", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 36, no. 3, March 1998 (1998-03-01), pages 662 - 668, XP002111947 *
J. YU AND J.B. KAPER: "Cloning and characterization of the eae gene of enterohemorhagic Escherichia coli O157:H7", MOLECULAR MICROBIOLOGY, vol. 6, no. 3, 1992, pages 411 - 417, XP002040900 *
JERSE A E ET AL: "THE EAE GENE OF ENTEROPATHOGENIC ESCHERICHIA COLI ENCODES A 94 -KILODALTON MEMBRANE PROTEIN, THE EXPRESSION OF WHICH IS INFLUENCED BY THE EAF PLASMID", INFECTION AND IMMUNITY, vol. 59, no. 12, 1 December 1991 (1991-12-01), pages 4302 - 4309, XP002040903 *

Also Published As

Publication number Publication date
GB9803322D0 (en) 1998-04-15
AU2535699A (en) 1999-08-30
EP1057035A2 (en) 2000-12-06
WO1999041614A2 (en) 1999-08-19
CA2320753A1 (en) 1999-08-19

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