+

WO1999041386A3 - Nouvelle endo-xylogalacturonase - Google Patents

Nouvelle endo-xylogalacturonase Download PDF

Info

Publication number
WO1999041386A3
WO1999041386A3 PCT/EP1999/000860 EP9900860W WO9941386A3 WO 1999041386 A3 WO1999041386 A3 WO 1999041386A3 EP 9900860 W EP9900860 W EP 9900860W WO 9941386 A3 WO9941386 A3 WO 9941386A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
xylogalacturonase
disclosed
novel
activity
Prior art date
Application number
PCT/EP1999/000860
Other languages
English (en)
Other versions
WO1999041386A2 (fr
Inventor
Petrus Johannes Alber Meeuwsen
Der Vlugt-Bergmans Cecile Van
Jean Paul Vincken
Gerrit Beldman
Alphons Gerard Joseph Voragen
Margareta Adriana Herweijer
Ooijen Albert Johannes Jos Van
Original Assignee
Dsm Nv
Petrus Johannes Alber Meeuwsen
Vlugt Bergmans Cecile Johanna
Jean Paul Vincken
Gerrit Beldman
Alphons Gerard Joseph Voragen
Margareta Adriana Herweijer
Ooijen Albert Johannes Joseph
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv, Petrus Johannes Alber Meeuwsen, Vlugt Bergmans Cecile Johanna, Jean Paul Vincken, Gerrit Beldman, Alphons Gerard Joseph Voragen, Margareta Adriana Herweijer, Ooijen Albert Johannes Joseph filed Critical Dsm Nv
Priority to BR9907831-7A priority Critical patent/BR9907831A/pt
Priority to EP99907529A priority patent/EP1054978A2/fr
Priority to AU27247/99A priority patent/AU2724799A/en
Publication of WO1999041386A2 publication Critical patent/WO1999041386A2/fr
Publication of WO1999041386A3 publication Critical patent/WO1999041386A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01171—Rhamnogalacturonan hydrolase (3.2.1.171), i.e. rhamnogalacturonase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/231—Pectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01099—Arabinan endo-1,5-alpha-L-arabinosidase (3.2.1.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

L'invention concerne des polypeptides possédant une nouvelle activité, notamment une activité d'endo-xylogalacturonase. Ces polypeptides peuvent dégrader la pectine trouvée dans des extraits de plantes et matières végétales, et notamment dans les régions 'velues' de polymères de pectine. Ces polypeptides peuvent notamment cliver un polymère d'acide galacturonique, au niveau de liaisons glycocidiques internes. L'invention concerne une nouvelle enzyme XghA, la séquence aminée de celle-ci ainsi que la séquence d'ADN codant pour celle-ci. On a fait s'exprimer ce polypeptide dans ces cellules de levure et on l'a utilisé pour traiter une matière végétale, notamment du soja et des jus de fruits, dans la préparation de denrées alimentaires comestibles.
PCT/EP1999/000860 1998-02-10 1999-02-09 Nouvelle endo-xylogalacturonase WO1999041386A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR9907831-7A BR9907831A (pt) 1998-02-10 1999-02-09 Endo-xilogalacturonase
EP99907529A EP1054978A2 (fr) 1998-02-10 1999-02-09 Nouvelle endo-xylogalacturonase
AU27247/99A AU2724799A (en) 1998-02-10 1999-02-09 Novel endo-xylogalacturonase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98300952.3 1998-02-10
EP98300952 1998-02-10

Publications (2)

Publication Number Publication Date
WO1999041386A2 WO1999041386A2 (fr) 1999-08-19
WO1999041386A3 true WO1999041386A3 (fr) 2000-01-20

Family

ID=8234656

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000860 WO1999041386A2 (fr) 1998-02-10 1999-02-09 Nouvelle endo-xylogalacturonase

Country Status (5)

Country Link
EP (1) EP1054978A2 (fr)
CN (1) CN1294633A (fr)
AU (1) AU2724799A (fr)
BR (1) BR9907831A (fr)
WO (1) WO1999041386A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2538793A4 (fr) * 2010-02-26 2014-07-23 Novozymes As Prétraitement enzymatique pour obtenir des fruits séchés
CN102762104B (zh) * 2010-02-26 2014-08-06 诺维信公司 用于制备果干的酶预处理
WO2014170498A1 (fr) 2013-04-19 2014-10-23 Danmarks Tekniske Universitet Production enzymatique de polysaccharides à partir de gomme adragante
CN107916268B (zh) * 2017-09-30 2021-10-22 武汉轻工大学 聚半乳糖醛酸裂解酶基因、重组表达载体、菌株、聚半乳糖醛酸裂解酶及其制备方法
CN114292832A (zh) * 2022-01-27 2022-04-08 大连海洋大学 一种嗜热耐高温多聚半乳糖醛酸酶MlPG28B、编码基因及制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0421919A2 (fr) * 1989-09-02 1991-04-10 Ciba-Geigy Ag Système d'expression fongique encodant la polygalacturonase
WO1994014966A1 (fr) * 1992-12-24 1994-07-07 Gist-Brocades N.V. CLONAGE ET EXPRESSION DU GENE DE L'EXO-POLYGALACTURONASE DE L'$i(ASPERGILLUS)
WO1995034223A1 (fr) * 1994-06-15 1995-12-21 Novo Nordisk A/S Stabilite des extraits troubles

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0421919A2 (fr) * 1989-09-02 1991-04-10 Ciba-Geigy Ag Système d'expression fongique encodant la polygalacturonase
WO1994014966A1 (fr) * 1992-12-24 1994-07-07 Gist-Brocades N.V. CLONAGE ET EXPRESSION DU GENE DE L'EXO-POLYGALACTURONASE DE L'$i(ASPERGILLUS)
WO1995034223A1 (fr) * 1994-06-15 1995-12-21 Novo Nordisk A/S Stabilite des extraits troubles

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN W P ET AL.: "Purification and some properties of beta-1,3-xylanases from Aspergillus terreus A-07. Endo-1,3-beta-D-xylanase isolation and characterization", AGRICULTURAL AND BIOLOGICAL CHEMISTRY., vol. 50, no. 5, 1986, TOKYO., JP, pages 1183 - 1194, XP002111869, ISSN: 0002-1369 *
RENARD C.M.G.C. ET AL.: "The xylose-rich pectins from pae hulls", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 21, no. 1-2, August 1997 (1997-08-01), AMSTERDAM NL, pages 155 - 162, XP002112536 *
SCHOLS H A ET AL: "A xylogalacturonan subunit present in the modified hairy regions of apple pectin", CARBOHYDRATE RESEARCH, vol. 279, 27 December 1995 (1995-12-27), amsterdam nl, pages 265-279, XP004018802, ISSN: 0008-6215 *

Also Published As

Publication number Publication date
BR9907831A (pt) 2000-10-24
AU2724799A (en) 1999-08-30
WO1999041386A2 (fr) 1999-08-19
EP1054978A2 (fr) 2000-11-29
CN1294633A (zh) 2001-05-09

Similar Documents

Publication Publication Date Title
AU2001262116A1 (en) Talaromyces emersonii beta-glucanases
JP2003527843A5 (fr)
CA2268043A1 (fr) Ameliorations dans la teneur de plantes en amidon ou la concernant
AU4208797A (en) Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
DE69509669D1 (de) Herstellung von gefrorenen fermentierten nahrungsmitteln mit gefrierschutz-polypeptide expremierenden mikroorganismen
IE830252L (en) Preparation of protein hydrolysates.
CN110951811B (zh) 一种糖基化蜂王浆抗氧化肽及其制备方法
IE851337L (en) Cell surface protein
WO1999041386A3 (fr) Nouvelle endo-xylogalacturonase
CN109260076A (zh) 一种樱桃发酵液及其制备方法和在化妆品中的应用
Salazar-Vega et al. Antihypertensive and antioxidant effects of functional foods containing chia (Salvia hispanica) protein hydrolysates
CA2192150A1 (fr) .beta.-fructofuranosidase; preparation et utilisation
AU1315299A (en) Use of liquid carbohydrate fermentation product in foods
EP0528822B1 (fr) Procede cohydrolytique pour la production de nouveaux extraits de proteines de levure et de proteines d'origine autre que la levure
Tchorbanov et al. Enzymatic hydrolysis of cell proteins in green algae Chlorella and Scenedesmus after extraction with organic solvents
RU93014928A (ru) Способ производства овощных лактоферментированных соков
EP0106309A3 (fr) Extraits biologiquement actifs, procédé pour leur obtention, agents pharmaceutiques et cosmétiques les contenant et leur utilisation comme additifs pour des produits alimentaires et stimulants
Cheetham Biotransformations: new routes to food ingredients
KR101489480B1 (ko) 고체상 발효를 통한 주름개선 활성을 갖는 녹용추출물 및 그 제조방법
RU97111688A (ru) Способ комплексного использования растительного сырья при производстве лечебно-профилактических продуктов для людей и животных
KR920004229B1 (ko) 감귤과피를 이용한 사료의 제조방법
KR102147106B1 (ko) 파프리카 함유 화장료 조성물 및 그 제조방법
PL334074A1 (en) Turbid fruit juices and methods of obtaining them
KR102260080B1 (ko) 탈지미세조류 효소분해물을 유효성분으로 포함하는 조성물
KR20200047359A (ko) 탈지 난황 단백질 가수분해물 제조 방법 및 이를 포함하는 항산화용 조성물

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99804431.8

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: PA/a/2000/007787

Country of ref document: MX

NENP Non-entry into the national phase

Ref country code: KR

WWE Wipo information: entry into national phase

Ref document number: 1999907529

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999907529

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 09601852

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: CA

WWW Wipo information: withdrawn in national office

Ref document number: 1999907529

Country of ref document: EP

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载