WO1998038336B1 - Procede pour identifier le site d'action d'agents chimiques xenobiotiques - Google Patents
Procede pour identifier le site d'action d'agents chimiques xenobiotiquesInfo
- Publication number
- WO1998038336B1 WO1998038336B1 PCT/US1998/003684 US9803684W WO9838336B1 WO 1998038336 B1 WO1998038336 B1 WO 1998038336B1 US 9803684 W US9803684 W US 9803684W WO 9838336 B1 WO9838336 B1 WO 9838336B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- detector
- bacteria
- stressor
- growth
- genotoxic
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 8
- 239000002676 xenobiotic agent Substances 0.000 title abstract 3
- 230000002034 xenobiotic effect Effects 0.000 title abstract 2
- 239000000126 substance Substances 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims abstract 25
- 231100000024 genotoxic Toxicity 0.000 claims abstract 10
- 230000001738 genotoxic effect Effects 0.000 claims abstract 10
- 108700008625 Reporter Genes Proteins 0.000 claims abstract 5
- 150000001875 compounds Chemical class 0.000 claims abstract 5
- 238000004020 luminiscence type Methods 0.000 claims abstract 4
- 235000016709 nutrition Nutrition 0.000 claims abstract 4
- 230000002068 genetic effect Effects 0.000 claims abstract 2
- 238000004448 titration Methods 0.000 claims abstract 2
- 108090000623 proteins and genes Proteins 0.000 claims 12
- 230000004927 fusion Effects 0.000 claims 6
- 230000002401 inhibitory effect Effects 0.000 claims 6
- 238000012544 monitoring process Methods 0.000 claims 6
- 235000015097 nutrients Nutrition 0.000 claims 6
- 231100000804 nongenotoxic Toxicity 0.000 claims 2
- 101100465873 Dictyostelium discoideum psiN gene Proteins 0.000 claims 1
- 101100285838 Escherichia coli (strain K12) hslV gene Proteins 0.000 claims 1
- 101100071977 Escherichia coli (strain K12) ibpA gene Proteins 0.000 claims 1
- 101100340221 Escherichia coli (strain K12) ibpB gene Proteins 0.000 claims 1
- 101100297484 Escherichia coli (strain K12) phnD gene Proteins 0.000 claims 1
- 101100463961 Escherichia coli (strain K12) phoH gene Proteins 0.000 claims 1
- 101100509674 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) katG3 gene Proteins 0.000 claims 1
- 101100278084 Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) dnaK1 gene Proteins 0.000 claims 1
- 101100091878 Plasmodium falciparum (isolate 3D7) rpoC2 gene Proteins 0.000 claims 1
- 101100084022 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) lapA gene Proteins 0.000 claims 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims 1
- 101100117145 Synechocystis sp. (strain PCC 6803 / Kazusa) dnaK2 gene Proteins 0.000 claims 1
- 101100123420 Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) hacB gene Proteins 0.000 claims 1
- 230000004075 alteration Effects 0.000 claims 1
- 101150036359 clpB gene Proteins 0.000 claims 1
- 101150074451 clpP gene Proteins 0.000 claims 1
- 101150043719 clpP1 gene Proteins 0.000 claims 1
- 101150102296 clpP2 gene Proteins 0.000 claims 1
- 101150052825 dnaK gene Proteins 0.000 claims 1
- 108010064177 glutamine synthetase I Proteins 0.000 claims 1
- -1 groESL Proteins 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 101150053330 grpE gene Proteins 0.000 claims 1
- 101150099805 htpG gene Proteins 0.000 claims 1
- 101150112675 htpX gene Proteins 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 claims 1
- 101150013110 katG gene Proteins 0.000 claims 1
- 101150110767 lysS gene Proteins 0.000 claims 1
- 101150112482 lysU gene Proteins 0.000 claims 1
- 101150029821 lysX gene Proteins 0.000 claims 1
- 101150064613 pepN gene Proteins 0.000 claims 1
- 101150009573 phoA gene Proteins 0.000 claims 1
- 101150058164 phoE gene Proteins 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 101150068531 psiD gene Proteins 0.000 claims 1
- 101150010280 psiE gene Proteins 0.000 claims 1
- 101150066994 psiF gene Proteins 0.000 claims 1
- 101150117145 psiH gene Proteins 0.000 claims 1
- 101150049598 psiK gene Proteins 0.000 claims 1
- 101150089853 psiR gene Proteins 0.000 claims 1
- 230000003362 replicative effect Effects 0.000 claims 1
- 101150029016 rpo3 gene Proteins 0.000 claims 1
- 101150102864 rpoD gene Proteins 0.000 claims 1
- 101150117326 sigA gene Proteins 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 238000005415 bioluminescence Methods 0.000 abstract 1
- 230000029918 bioluminescence Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
Abstract
Cette invention se rapporte à un procédé permettant de découvrir le site d'action de composés xénobiotiques. Ce procédé consiste à soumettre une cellule détecteur comprenant un promoteur sensible aux agents génotoxiques liés opérationnellement à un complexe de gènes reporteurs luminescents. L'exposition des bactéries détecteurs à des composés qui sont génotoxiques produit un accroîssement de la luminescence. Ces composés sont groupés et peuvent être analysés pour que leur activité pharmaceutique soit déterminée. L'exposition des bactéries détecteurs à des agents xénobiotiques actifs qui ne sont pas génotoxiques produit une inhibition de la bioluminescence ou de la luminescence stable. En soumettant les bactéries détecteurs à un tri, tel qu'un titrage génétique ou d'inversion nutritionnelle, on dévoile le site d'action du composé en question.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IL13133698A IL131336A0 (en) | 1997-02-28 | 1998-02-26 | A method for identifying the site of action of xenobiotic chemicals |
| EP98907633A EP0972074A1 (fr) | 1997-02-28 | 1998-02-26 | Procede pour identifier le site d'action d'agents chimiques xenobiotiques |
| CA002279576A CA2279576A1 (fr) | 1997-02-28 | 1998-02-26 | Procede pour identifier le site d'action d'agents chimiques xenobiotiques |
| JP53781298A JP2001514501A (ja) | 1997-02-28 | 1998-02-26 | 生体異物化学品の作用部位の同定のための方法 |
| AU63390/98A AU6339098A (en) | 1997-02-28 | 1998-02-26 | A method for identifying the site of action of xenobiotic chemicals |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3958297P | 1997-02-28 | 1997-02-28 | |
| US60/039,582 | 1997-02-28 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1998038336A1 WO1998038336A1 (fr) | 1998-09-03 |
| WO1998038336B1 true WO1998038336B1 (fr) | 1998-11-05 |
Family
ID=21906239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1998/003684 WO1998038336A1 (fr) | 1997-02-28 | 1998-02-26 | Procede pour identifier le site d'action d'agents chimiques xenobiotiques |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0972074A1 (fr) |
| JP (1) | JP2001514501A (fr) |
| AU (1) | AU6339098A (fr) |
| CA (1) | CA2279576A1 (fr) |
| IL (1) | IL131336A0 (fr) |
| WO (1) | WO1998038336A1 (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ580248A (en) | 2004-04-30 | 2012-01-12 | Dow Agrosciences Llc | Plants comprising herbicide resistance gene encoding aryloxyalkanoate dioxygenase |
| DE102004026286B4 (de) * | 2004-05-28 | 2007-10-04 | Rene Girod Gmbh | Motorradständer |
| CA2628263C (fr) | 2005-10-28 | 2016-12-13 | Dow Agrosciences Llc | Genes de resistance aux herbicides aryloxyalkanoate auxin et leurs utilisations |
| JP2007312750A (ja) * | 2006-05-29 | 2007-12-06 | Daikin Ind Ltd | 改良された化学物質の検出方法 |
| JP2009039132A (ja) * | 2008-10-14 | 2009-02-26 | Daikin Ind Ltd | 改良された化学物質の検出方法 |
| KR20220019763A (ko) * | 2019-06-11 | 2022-02-17 | 포테페스트 리미티드 | 신규한 비코딩 헤테로사이클릭 아미노산(nchaa) 및 그것의 제초제로서의 용도 |
| CN110241129B (zh) * | 2019-06-18 | 2022-11-15 | 山东大学 | 一种发光报告系统及其在抗生素发现筛选中的应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5786162A (en) * | 1991-03-01 | 1998-07-28 | Vito | Fused genes and their use for determining the presence of metals or of xenobiotic compounds |
| IL107815A (en) * | 1992-12-04 | 2003-12-10 | Du Pont | Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing |
| AU692434B2 (en) * | 1993-01-21 | 1998-06-11 | President And Fellows Of Harvard College | Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound |
| DE69413491T2 (de) * | 1993-10-22 | 1999-03-11 | Kabushiki Kaisha Toyota Chuo Kenkyusho, Aichi | Verfahren zum Nachweis von Mutagenen unter Verwendung eines Lumineszensgens |
| JPH10509049A (ja) * | 1994-11-23 | 1998-09-08 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 毒物検出のための凍結乾燥生物発光細菌試薬 |
| DE19549417C2 (de) * | 1995-11-29 | 1998-08-13 | Deutsch Zentr Luft & Raumfahrt | Verfahren zur luminometrischen Bestimmung der mutagenen und/oder karzinogenen Potenz einer zu untersuchenden Probe oder eines zu untersuchenden Faktors (Agens) |
| JP4371432B2 (ja) * | 1996-04-25 | 2009-11-25 | ヴラームス インステリング ヴール テクノロギシュ オンデルゾーク （ヴイアイティーオー） | サンプルの遺伝毒性及び変異原性の両方、及び遺伝毒性の速度を測定するための組換え核酸配列及び方法 |
| WO1998021347A1 (fr) * | 1996-11-15 | 1998-05-22 | E.I. Du Pont De Nemours And Company | Procede tres sensible de detection sur de faibles volumes des atteintes a l'environnement |
-
1998
- 1998-02-26 CA CA002279576A patent/CA2279576A1/fr not_active Abandoned
- 1998-02-26 EP EP98907633A patent/EP0972074A1/fr not_active Withdrawn
- 1998-02-26 AU AU63390/98A patent/AU6339098A/en not_active Abandoned
- 1998-02-26 IL IL13133698A patent/IL131336A0/xx unknown
- 1998-02-26 JP JP53781298A patent/JP2001514501A/ja active Pending
- 1998-02-26 WO PCT/US1998/003684 patent/WO1998038336A1/fr not_active Application Discontinuation
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