+

WO1998035058A3 - Procede ameliore de detection et de quantification de molecules d'acides nucleiques - Google Patents

Procede ameliore de detection et de quantification de molecules d'acides nucleiques Download PDF

Info

Publication number
WO1998035058A3
WO1998035058A3 PCT/US1998/001471 US9801471W WO9835058A3 WO 1998035058 A3 WO1998035058 A3 WO 1998035058A3 US 9801471 W US9801471 W US 9801471W WO 9835058 A3 WO9835058 A3 WO 9835058A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
quantification
detection
improved process
Prior art date
Application number
PCT/US1998/001471
Other languages
English (en)
Other versions
WO1998035058A2 (fr
Inventor
James D Thompson
Original Assignee
Ribozyme Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribozyme Pharm Inc filed Critical Ribozyme Pharm Inc
Priority to AU63173/98A priority Critical patent/AU6317398A/en
Publication of WO1998035058A2 publication Critical patent/WO1998035058A2/fr
Publication of WO1998035058A3 publication Critical patent/WO1998035058A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de mesure des quantités relatives de deux molécules d'acides nucléiques différentes ou davantage dans un système biologique au moyen d'une amplification en chaîne par polymérase multiplex compétitive.
PCT/US1998/001471 1997-02-07 1998-01-27 Procede ameliore de detection et de quantification de molecules d'acides nucleiques WO1998035058A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU63173/98A AU6317398A (en) 1997-02-07 1998-01-27 Improved process for detection and quantification of nucleic acid molecules

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3784197P 1997-02-07 1997-02-07
US60/037,841 1997-02-07
US99373197A 1997-12-18 1997-12-18
US08/993,731 1997-12-18

Publications (2)

Publication Number Publication Date
WO1998035058A2 WO1998035058A2 (fr) 1998-08-13
WO1998035058A3 true WO1998035058A3 (fr) 1998-09-17

Family

ID=26714548

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001471 WO1998035058A2 (fr) 1997-02-07 1998-01-27 Procede ameliore de detection et de quantification de molecules d'acides nucleiques

Country Status (1)

Country Link
WO (1) WO1998035058A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964847B1 (en) 1999-07-14 2005-11-15 Packard Biosciences Company Derivative nucleic acids and uses thereof
EP1194592B1 (fr) * 1999-07-14 2008-09-24 Packard Bioscience Company Acides nucleiques modifies et utilisations associees
TWI237663B (en) * 2000-12-12 2005-08-11 Masumi Abe Method of analyzing expression of gene
US20060035222A1 (en) * 2002-01-15 2006-02-16 Knut Rudi Methods of nucleic acid amplification
FI20040723A0 (fi) 2004-05-26 2004-05-26 Orpana Aarne Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
EP0628640A1 (fr) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Amplification simultanée de cibles multiples

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive
EP0628640A1 (fr) * 1993-06-04 1994-12-14 Becton, Dickinson and Company Amplification simultanée de cibles multiples

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BABU ET AL.: "LIMITATIONS AND MODIFICATIONS OF QUANTITATIVE POLYMERASE CHAN REACTION", J. IMMUN. METH., vol. 165, 1993, pages 207 - 216, XP002071877 *
CELI F S ET AL: "A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR", NUCLEIC ACIDS RESEARCH, vol. 21, no. 4, 25 February 1993 (1993-02-25), pages 1047, XP000349930 *
FERRE F ET AL: "QUANTITATIVE PCR: AN OVERVIEW", 1994, POLYMERASE CHAIN REACTION, PAGE(S) 67 - 88, MULLIS K B;FERRE F; GIBBS R A, XP002017884 *
KELLER AND MANAK: "DNA PROBES", 1994, STOCKTON PRESS, US, NY, XP002071878, 208660 *
REISCHL U ET AL: "QUANTITATIVE PCR A SURVEY OF THE PRESENT TECHNOLOGY", MOLECULAR BIOTECHNOLOGY, vol. 3, 1995, pages 55 - 71, XP000600241 *
SECCHIERO P ET AL: "QUANTITATIVE PCR FOR HUMAN HERPESVIRUSES 6 AND 7", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 33, no. 8, 1 August 1995 (1995-08-01), pages 2124 - 2130, XP000564243 *
SIEBERT AND LARRICK: "COMPETITVE PCR", NATURE, vol. 359, 1992, pages 557 - 558, XP002071876 *
ZIMMERMANN K ET AL: "TECHNICAL ASPECTS OF QUANTITATIVE COMPETITIVE PCR", BIOTECHNIQUES, vol. 21, no. 2, 1 August 1996 (1996-08-01), pages 268 - 270, 272, 274 - 279, XP000597689 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7125660B2 (en) 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same

Also Published As

Publication number Publication date
WO1998035058A2 (fr) 1998-08-13

Similar Documents

Publication Publication Date Title
EP0733905A3 (fr) Système pour un essai diagnostique d'acide nucléique
EP0639647A3 (fr) Procédé pour déterminer des séquences d'acides nucléiques.
AU3199699A (en) Modified nucleotides and methods useful for nucleic acid sequencing
CA2182517A1 (fr) Extension d'amorces ligase/polymerase-a mediation de polymorphismes de mononucleotides et son utilisation dans des analyses genetiques
CA2039517A1 (fr) Amplification du signal d'une sonde d'adn
WO1996013612A3 (fr) Compositions et procedes de detection et de quantification simultanees de sequences specifiques multiples d'acide nucleique
ZA200107497B (en) Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays.
EP0742287B8 (fr) Sondes d'acide nucleique modifiés
CA2307177A1 (fr) Procedes de detection de contamination dans le diagnostic moleculaire utilisant la reaction pcr
HUP9802024A3 (en) Altered thermostable dna polymerases for sequencing
CA2067909A1 (fr) Methodes homogenes d'amplification et de detection de l'acide nucleique
EP0986651A4 (fr) Polymerases pour l'analyse et le typage de fragments d'acide nucleique polymorphes et leurs utilisations
AU6412799A (en) Reverse displacement assay for detection of nucleic acid sequences
AU6256896A (en) Methods for nucleic acid detection, sequencing, and cloning using exonuclease
FI953990A0 (fi) Menetelmä nukleiinihappomonistusreaktiotuotteen tekemiseksi kykenemät-tömäksi olemaan lisämonistuksen kohteena, diagnostinen määritysmenetelmä, jossa tätä menetelmää käytetään, ja menetelmän tai määrityksen toteutukseen sopiva testipakkaus ja säiliö
AU7595896A (en) Homogeneous dna probe titration assay
WO1997007235A3 (fr) Methode integrale d'amplification d'acide nucleique
WO1998014611A3 (fr) Detection et identification d'enterovirus non poliovirus
WO1998035058A3 (fr) Procede ameliore de detection et de quantification de molecules d'acides nucleiques
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
AU7813698A (en) Nucleic acid detection
SE9500589D0 (sv) Single nucleic acid base detection on solid substrates in single molecule DNA sequencing
WO2004000995A3 (fr) Procedes, sondes et molecules accessoires permettant de detecter des polymorphismes nucleotidiques uniques
CA2222513A1 (fr) Agents s'agglutinant specifiquement a la biotine pour detecter des bases endommagees de l'acide nucleique
WO1999066077A3 (fr) Methodes pour detecter des acides nucleiques

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP MX

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: PAT. BUL. 32/98 UNDER (30) REPLACE "NOT FURNISHED" BY "08/993731"

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: JP

Ref document number: 1998534760

Format of ref document f/p: F

122 Ep: pct application non-entry in european phase
点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载