+

WO1998032847A2 - Caracterisation du transcriptome de levure - Google Patents

Caracterisation du transcriptome de levure Download PDF

Info

Publication number
WO1998032847A2
WO1998032847A2 PCT/US1998/001216 US9801216W WO9832847A2 WO 1998032847 A2 WO1998032847 A2 WO 1998032847A2 US 9801216 W US9801216 W US 9801216W WO 9832847 A2 WO9832847 A2 WO 9832847A2
Authority
WO
WIPO (PCT)
Prior art keywords
gene
group
norf
phase
yeast
Prior art date
Application number
PCT/US1998/001216
Other languages
English (en)
Other versions
WO1998032847A3 (fr
Inventor
Victor E. Velculescu
Bert Vogelstein
Kenneth W. Kinzler
Original Assignee
The Johns Hopkins University School Of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Johns Hopkins University School Of Medicine filed Critical The Johns Hopkins University School Of Medicine
Priority to CA002278645A priority Critical patent/CA2278645A1/fr
Priority to JP53211798A priority patent/JP2001509017A/ja
Priority to AU59280/98A priority patent/AU749606C/en
Priority to EP98902680A priority patent/EP0970202A2/fr
Publication of WO1998032847A2 publication Critical patent/WO1998032847A2/fr
Publication of WO1998032847A3 publication Critical patent/WO1998032847A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces

Definitions

  • Locus and Description denote the locus name, and functional description of each ORF, respectively; Copies/cell represents the abundance of each transcript in the SAGE library, assuming 15,000 total transcripts per cell and 60,633 ascertained transcripts.
  • ORF Nonannotated ORF
  • nocodazole 15ug/ml was added to early log phase cells and the culture was incubated for an additional 100 minutes at 30°C.
  • Harvested cells were washed once with water prior to freezing at -70 °C.
  • the growth states of the harvested cells were confirmed by microscopic and flow cytometric analyses (Basrai et al., 1996).
  • GAL3 carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL 10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases. Mol Cell Biol 8, 3439-3447.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne des gènes de levure qui sont exprimés de manière différentielle au cours du cycle cellulaire. On peut les utiliser pour étudier, modifier et surveiller le cycle cellulaire d'une cellule eucaryote; pour obtenir des homologues humains impliqués dans la régulation du cycle cellulaire, et pour identifier des agents antifongiques. On peut les former sous forme de réseaux sur des supports solides pour étudier un transcriptome de cellule dans diverses conditions.
PCT/US1998/001216 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure WO1998032847A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002278645A CA2278645A1 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure
JP53211798A JP2001509017A (ja) 1997-01-23 1998-01-22 イーストトランスクリプトームのキャラクタリゼーション
AU59280/98A AU749606C (en) 1997-01-23 1998-01-22 Characterization of the yeast transcriptome
EP98902680A EP0970202A2 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3591797P 1997-01-23 1997-01-23
US60/035,917 1997-01-23

Publications (2)

Publication Number Publication Date
WO1998032847A2 true WO1998032847A2 (fr) 1998-07-30
WO1998032847A3 WO1998032847A3 (fr) 1998-11-26

Family

ID=21885540

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/001216 WO1998032847A2 (fr) 1997-01-23 1998-01-22 Caracterisation du transcriptome de levure

Country Status (5)

Country Link
EP (1) EP0970202A2 (fr)
JP (1) JP2001509017A (fr)
AU (1) AU749606C (fr)
CA (1) CA2278645A1 (fr)
WO (1) WO1998032847A2 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000077214A3 (fr) * 1999-06-16 2001-05-03 Univ Johns Hopkins Caracterisation du transcriptome d'une levure
FR2821087A1 (fr) * 2001-02-16 2002-08-23 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
US7270977B2 (en) 2001-12-11 2007-09-18 Bayer Cropscience Ag Polypeptides for identifying fungicidally active compounds
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
US8283158B2 (en) 2002-11-25 2012-10-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005263993B2 (en) * 2004-07-23 2009-11-12 Ge Healthcare Uk Limited Cell cycle reporting cell line
EP3370744A4 (fr) * 2015-11-02 2019-04-17 Orig3N, Inc. Le blocage du cycle cellulaire améliore l'efficacité de production de cellules souches pluripotentes induites

Non-Patent Citations (17)

* Cited by examiner, † Cited by third party
Title
A. GOFFEAU ET AL.: "Life with 6000 genes" SCIENCE, vol. 274, 25 October 1996, AAAS,WASHINGTON,DC,US, pages 546-567, XP002070912 cited in the application *
BISHOP D K ET AL: "DMC1: A MEIOSIS-SPECIFIC YEAST HOMOLOG OF E. COLI RECA REQUIRED FORRECOMBINATION, SYNAPTONEMAL COMPLEX FORMATION, AND CELL CYCLE PROGRESSION" CELL, vol. 69, 1 May 1992, pages 439-456, XP000676406 *
BURNS N ET AL: "LARGE-SCALE ANALYSIS OF GENE EXPRESSION, PROTEIN LOCALIZATION, AND GENE DISRUPTION IN SACCHAROMYCES CERECISIAE" GENES AND DEVELOPMENT, vol. 8, no. 9, 1 May 1994, pages 1087-1105, XP000569086 *
C. FRANKHAUSER ET AL.: "The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitose" CELL, vol. 82, 11 August 1995, CELL PRESS,CAMBRIDGE,MA,US;, pages 435-444, XP002070907 *
C. PRICE ET AL.: "A general approach to the isolation of cell cycle-regulated genes in the budding yeast, Saccharomyces cerevisiae" J. MOL. BIOL., vol. 218, no. 3, 5 April 1991, ACADEMIC PRESS,US, pages 543-556, XP002070908 *
J.H. TOYN ET AL.: "The cell-cycle-regulated yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control" GENE, vol. 104, 1991, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 63-70, XP002070905 *
K. NASMYTH: "Control of the yeast cell cycle by the Cdc28 protein kinase" CURRENT OPINION IN CELL BIOLOGY, vol. 5, no. 2, April 1993, CURRENT BIOLOGY, LTD., US, pages 166-179, XP002071095 *
LOCKHART D J ET AL: "EXPRESSION MONITORING BY HYBRIDIZATION TO HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS" BIO/TECHNOLOGY, vol. 14, no. 13, December 1996, pages 1675-1680, XP002022521 cited in the application *
M. SCHENA ET AL.: "Paralel human genome analysis: Microarray-based expression monitoring of 1000 genes" PROC. NATL. ACAD. SCI., vol. 93, October 1996, NATL. ACAD. SCI.,WASHINGTON,DC,US;, pages 10614-10619, XP002070911 *
R.M. GOLDSTEYN ET AL.: "Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5" J. CELL SCIENCE, vol. 107, no. 6, 1994, THE COMPANY OF BIOLOGISTS LIMITED, GB, pages 1509-1517, XP002070910 *
S.J. ELLEDGE AND R.W. DAVIS: "DNA damage induction of ribonucleotide reductase" MOL. CELL. BIOL., vol. 9, no. 11, November 1989, ASM WASHINGTON, DC,US, pages 4932-4940, XP002070906 cited in the application *
SCHENA M ET AL: "QUANTITATIVE MONITORING OF GENE EXPRESSION PATTERNS WITH A COMPLEMENTARY DNA MICROARRAY" SCIENCE, vol. 270, no. 5235, 20 October 1995, pages 467-470, XP000644675 cited in the application *
SHALON D ET AL: "A DNA MICROARRAY SYSTEM FOR ANALYZING COMPLEX DNA SAMPLES USING TWO-COLOR FLUORESCENT PROBE HYBRIDIZATION" GENOME RESEARCH, vol. 6, no. 7, 1 July 1996, pages 639-645, XP000597095 *
T. YASUGI AND P.M. HOWLEY: "Identification of the structural and functional human homolog of the yeast ubiquitin conjugated enzyme UBC9" NUCLEIC ACIDS RESEARCH, vol. 24, no. 11, 1996, IRL PRESS LIMITED,OXFORD,ENGLAND, pages 2005-2010, XP002070909 cited in the application *
V.E.VCELCULESCU ET AL.: "Characterization of the yeast transcriptome" CELL, vol. 88, 24 January 1997, CELL PRESS,CAMBRIDGE,MA,US;, pages 243-251, XP002070903 *
VELCULESCU V E ET AL: "SERIAL ANALYSIS OF GENE EXPRESSION" SCIENCE, vol. 270, 20 October 1995, pages 484-487, XP002053721 cited in the application *
Y. KYU JANG ET AL.: "Differential expression of the rhp51+ gene, a recA and RAD51 homolog from the fission yeast Schizosaccharomyces pombe" GENE, vol. 169, 1996, ELSEVIER SCIENCE PUBLISHERS,B.V.,AMSTERDAM,NL;, pages 125-130, XP002070904 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7504493B2 (en) 1997-01-23 2009-03-17 The John Hopkins University Characterization of the yeast transcriptome
WO2000077214A3 (fr) * 1999-06-16 2001-05-03 Univ Johns Hopkins Caracterisation du transcriptome d'une levure
FR2821087A1 (fr) * 2001-02-16 2002-08-23 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
WO2002066676A3 (fr) * 2001-02-16 2002-10-17 Centre Nat Rech Scient Procede d'analyse qualitative et quantitative d'une population d'acides nucleiques contenus dans un echantillon
US7270977B2 (en) 2001-12-11 2007-09-18 Bayer Cropscience Ag Polypeptides for identifying fungicidally active compounds
US8283158B2 (en) 2002-11-25 2012-10-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array

Also Published As

Publication number Publication date
CA2278645A1 (fr) 1998-07-30
AU749606C (en) 2007-05-17
AU749606B2 (en) 2002-06-27
JP2001509017A (ja) 2001-07-10
EP0970202A2 (fr) 2000-01-12
AU5928098A (en) 1998-08-18
WO1998032847A3 (fr) 1998-11-26

Similar Documents

Publication Publication Date Title
Velculescu et al. Characterization of the yeast transcriptome
US7504493B2 (en) Characterization of the yeast transcriptome
Brewster et al. An osmosensing signal transduction pathway in yeast
EP0692025B1 (fr) Cellules de levure traitees pour produire des substituts de proteines du systeme de pheromones, et leurs emplois
Kasten et al. Identification of the Saccharomyces cerevisiae genes STB1–STB5 encoding Sin3p binding proteins
Tolkacheva et al. Cloning of a Cryptococcus neoformans gene, GPA1, encoding a G-protein alpha-subunit homolog
Nugent et al. Gene expression during Ustilago maydis diploid filamentous growth: EST library creation and analyses
EP1190044A2 (fr) Caracterisation du transcriptome d'une levure
AU749606C (en) Characterization of the yeast transcriptome
Mosrin et al. The RPC31 gene of Saccharomyces cerevisiae encodes a subunit of RNA polymerase C (III) with an acidic tail
US20030073163A1 (en) Libraries of expressible gene sequences
Vollmer et al. [15] High expression cloning, purification, and assay of Ypt—GTPase-activating proteins
Schlecht et al. Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast
Naitou et al. Expression profiles of transcripts from 126 open reading frames in the entire chromosome VI of Saccharomyces cerevisiae by systematic northern analyses
Kang et al. Ordered differential display from Cryphonectria parasitica
Dai et al. Characterization of a novel gene encoding zinc finger domains identified from expressed sequence tags (ESTs) of a human heart cDNA database
Boles Yeast as a model system for studying glucose transport
WO2000009695A2 (fr) Cibles de medicaments dans le candida albicans
JP2002525073A (ja) Ｃ．アルビカンス由来の必須遺伝子及び当該遺伝子を用いる抗真菌性物質のスクリーニング方法
AU759732B2 (en) Method for screening antimycotic substances using essential genes from S. cerevisiae
KR100239143B1 (ko) 전사조절 매개체 단백질(Med6p), 그것의 돌연변이 단백질(med6p)과 이 돌연변이 단백질을 코딩하는 유전자 및 야생형 및 돌연변이 유전자에 의해 형질전환된 균주
Pereira Aromatic amino acid biosynthesis in Candida albicans
Gutiérrez Ilabaca Identification of unstable transcripts in Arabidopsis by cDNA microarray analysis: Rapid decay is associated with a group of touch-and specific clock-controlled genes
Hall et al. An HMG Protein, Hmo1, Associates with
US20040229276A1 (en) Method for screening antimycotic substances substances using essential genes from S. cerevisiae

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM GW HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase in:

Ref country code: CA

Ref document number: 2278645

Kind code of ref document: A

Format of ref document f/p: F

Ref document number: 2278645

Country of ref document: CA

ENP Entry into the national phase in:

Ref country code: JP

Ref document number: 1998 532117

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 59280/98

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1998902680

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1998902680

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 59280/98

Country of ref document: AU

点击 这是indexloc提供的php浏览器服务，不要输入任何密码和下载