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WO1998032843A3 - Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants - Google Patents

Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants Download PDF

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Publication number
WO1998032843A3
WO1998032843A3 PCT/US1998/000738 US9800738W WO9832843A3 WO 1998032843 A3 WO1998032843 A3 WO 1998032843A3 US 9800738 W US9800738 W US 9800738W WO 9832843 A3 WO9832843 A3 WO 9832843A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
plants
flower formation
expression
Prior art date
Application number
PCT/US1998/000738
Other languages
French (fr)
Other versions
WO1998032843A2 (en
Inventor
Michael G Zwick
James A Mcswiggen
Original Assignee
Ribozyme Pharm Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ribozyme Pharm Inc filed Critical Ribozyme Pharm Inc
Priority to AU59183/98A priority Critical patent/AU5918398A/en
Publication of WO1998032843A2 publication Critical patent/WO1998032843A2/en
Publication of WO1998032843A3 publication Critical patent/WO1998032843A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/122—Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

An enzymatic nucleic acid molecule with RNA cleaving activity, wherein the nucleic acid molecule modulates the expression of a gene involved in the biosynthesis of alkaloid compounds and flower formation in a plant. A transgenic plant comprising nucleic acids encoding for an enzymatic nucleic acid molecule with RNA cleaving activity, wherein the nucleic acid molecule modulates the expression of a gene involved in the biosynthesis of alkaloid compounds of flower formation in a plant. An enzymatic nucleic acid molecule with RNA cleaving activity, wherein the nucleic acid molecule modulates the expression of solanidine UDP-glucose glucosyl-transferase gene or citrate synthase in plants.
PCT/US1998/000738 1997-01-28 1998-01-14 Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants WO1998032843A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU59183/98A AU5918398A (en) 1997-01-28 1998-01-14 Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US3654597P 1997-01-28 1997-01-28
US3659997P 1997-01-28 1997-01-28
US60/036,545 1997-01-28
US60/036,599 1997-01-28
US08/979,416 1997-11-26
US97941697A 1997-11-27 1997-11-27

Publications (2)

Publication Number Publication Date
WO1998032843A2 WO1998032843A2 (en) 1998-07-30
WO1998032843A3 true WO1998032843A3 (en) 1998-11-19

Family

ID=27365053

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/000738 WO1998032843A2 (en) 1997-01-28 1998-01-14 Compositions and method for modulation of alkaloid biosynthesis and flower formation in plants

Country Status (2)

Country Link
AU (1) AU5918398A (en)
WO (1) WO1998032843A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP795198A0 (en) 1998-12-24 1999-01-28 Commonwealth Scientific And Industrial Research Organisation Miniribozymes active at low magnesium ion concentrations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010328A2 (en) * 1995-07-13 1997-03-20 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997010328A2 (en) * 1995-07-13 1997-03-20 Ribozyme Pharmaceuticals, Inc. Compositions and method for modulation of gene expression in plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BOROVKOV, A.Y. ET AL.: "Effect of expression of UDP-glucose pyrophosphorylase ribozyme and antisense RNAs on the enzyme activity and carbohydrate composition on field-grown transgenic potato plants.", J. PLANT PHYSIOL., vol. 147, 1996, pages 644 - 651, XP002068476 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US7408098B2 (en) 1997-06-12 2008-08-05 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US7425670B2 (en) 1997-06-12 2008-09-16 North Carolina State University Methods and compositions for protein production in tobacco plants with reduced nicotine
US7605308B2 (en) 1997-06-12 2009-10-20 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US6907887B2 (en) 2001-06-08 2005-06-21 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco

Also Published As

Publication number Publication date
WO1998032843A2 (en) 1998-07-30
AU5918398A (en) 1998-08-18

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