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WO1998021329A1 - Nouvelle proteine secretoire de parotides humaines - Google Patents

Nouvelle proteine secretoire de parotides humaines Download PDF

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Publication number
WO1998021329A1
WO1998021329A1 PCT/US1997/020651 US9720651W WO9821329A1 WO 1998021329 A1 WO1998021329 A1 WO 1998021329A1 US 9720651 W US9720651 W US 9720651W WO 9821329 A1 WO9821329 A1 WO 9821329A1
Authority
WO
WIPO (PCT)
Prior art keywords
hpsp
leu
sequence
sequences
seq
Prior art date
Application number
PCT/US1997/020651
Other languages
English (en)
Inventor
Olga Bandman
Surya K. Goli
Original Assignee
Incyte Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Incyte Pharmaceuticals, Inc. filed Critical Incyte Pharmaceuticals, Inc.
Priority to AU54355/98A priority Critical patent/AU5435598A/en
Publication of WO1998021329A1 publication Critical patent/WO1998021329A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4713—Autoimmune diseases, e.g. Insulin-dependent diabetes mellitus, multiple sclerosis, rheumathoid arthritis, systemic lupus erythematosus; Autoantigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • stringent conditions is the “stringency” which occurs within a range from about Tm-5°C (5°C below the melting temperature (Tm) of the probe) to about 20°C to 25 °C below Tm.
  • Tm melting temperature
  • the stringency of hybridization may be altered in order to identify or detect identical or related polynucleotide sequences.
  • “Alterations” in the polynucleotide of SEQ ID NO: 2, as used herein, comprise any alteration in the sequence of polynucleotides encoding HPSP including deletions, insertions, and point mutations that may be detected using hybridization assays.
  • antibody refers to intact molecules as well as fragments thereof, such as Fa, F(ab') 2 , and Fv, which are capable of binding the epitopic determinant.
  • humanized antibody refers to antibody molecules in which amino acids have been replaced in the non-antigen binding regions in order to more closely resemble a human antibody, while still retaining the original binding ability.
  • nucleotide sequences which encode HPSP and its variants are preferably capable of hybridizing to the nucleotide sequence of the naturally occurring HPSP under appropriately selected conditions of stringency, it may be advantageous to produce nucleotide sequences encoding HPSP or its derivatives possessing a substantially different codon usage. Codons may be selected to increase the rate at which expression of the peptide occurs in a particular prokaryotic or eukaryotic host in accordance with the frequency with which particular codons are utilized by the host.
  • Altered nucleic acid sequences encoding HPSP which are encompassed by the invention include deletions, insertions, or substitutions of different nucleotides resulting in a polynucleotide that encodes the same or a functionally equivalent HPSP.
  • the encoded protein may also contain deletions, insertions, or substitutions of amino acid residues which produce a silent change and result in a functionally equivalent HPSP. Deliberate amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues as long as the biological activity of HPSP is retained.
  • the basis of the search is the product score which is defined as: % sequence identity x % maximum BLAST score
  • Step 2 94° C for 20 sec
  • Step 5 Repeat steps 2-4 for an additional 29 cycles Step 6 72° C for 180 sec
  • HPSP expression of HPSP is accomplished by subcloning the cDNAs into appropriate vectors and transforming the vectors into host cells.
  • the cloning vector, pSport previously used for the generation of the cDNA library is used to express HPSP in E. coli. Upstream of the cloning site, this vector contains a promoter for ⁇ -galactosidase, followed by sequence containing the amino-terminal Met, and the subsequent seven residues of ⁇ -galactosidase. Immediately following these eight residues is a bacteriophage promoter useful for transcription and a linker containing a number of unique restriction sites.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Rheumatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Rehabilitation Therapy (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne une protéine sécrétoire de parotides humaines (HPSP) et des polynucléotides qui identifient et codent ladite protéine. L'invention porte également sur des vecteurs d'expression mis au point par génie génétique, sur des cellules hôtes renfermant les séquences nucléotidiques codant HPSP et sur un procédé de préparation de HPSP. L'invention a également trait à des agonistes, des anticorps, ou des antagonistes se liant spécifiquement à HPSP, et leur utilisation pour la prévention et le traitement des maladies associées à l'expression de HPSP. En outre, l'invention concerne l'utilisation de molécules antisens des polynucléotides codant HPSP pour le traitement des maladies associées à l'expression de HPSP. L'invention porte enfin sur des méthodes diagnostiques utilisant le polynucléotide, des fragments ou le complément dudit polynucléotide, et des anticorps se liant spécifiquement à HPSP.
PCT/US1997/020651 1996-11-14 1997-11-07 Nouvelle proteine secretoire de parotides humaines WO1998021329A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU54355/98A AU5435598A (en) 1996-11-14 1997-11-07 Novel human parotid secretory protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74928896A 1996-11-14 1996-11-14
US08/749,288 1996-11-14

Publications (1)

Publication Number Publication Date
WO1998021329A1 true WO1998021329A1 (fr) 1998-05-22

Family

ID=25013108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/020651 WO1998021329A1 (fr) 1996-11-14 1997-11-07 Nouvelle proteine secretoire de parotides humaines

Country Status (2)

Country Link
AU (1) AU5435598A (fr)
WO (1) WO1998021329A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008138001A2 (fr) * 2007-05-08 2008-11-13 University Of Louisville Research Foundation Peptides synthétiques et mimétiques peptidiques

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
H.O.MADSEN AND J.P. HJORTH: "Molecular cloning of mouse PSP mRNA", NUCLEIC ACIDS RESEARCH, vol. 13, no. 1, 11 January 1985 (1985-01-11), OXFORD GB, pages 1 - 13, XP002058086 *
K.N. PRASAD ET AL.: "Characterization of human and rat immortalized clones of parotid acinar cells with respect to specific proteins and their mRNAs, and receptor-linked adenylate cyclase", IN VITRO CELL DEV.BIOL. ANIM., vol. 31, no. 10, November 1995 (1995-11-01), ISSN 1071-2690, pages 767 - 772, XP002058351 *
L. MIRELS AND W.D. BALL: "Neonatal rat submandibular gland protein SMG-A and parotid secretory protein are alternatively regulated members of a salivary protein multigene family", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 4, 5 February 1992 (1992-02-05), MD US, pages 2679 - 2687, XP002058087 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008138001A2 (fr) * 2007-05-08 2008-11-13 University Of Louisville Research Foundation Peptides synthétiques et mimétiques peptidiques
WO2008138001A3 (fr) * 2007-05-08 2008-12-24 Univ Louisville Res Found Peptides synthétiques et mimétiques peptidiques
US8569449B2 (en) 2007-05-08 2013-10-29 University Of Louisville Research Foundation, Inc. Synthetic peptides and peptide mimetics
US9914750B2 (en) 2007-05-08 2018-03-13 University Of Louisville Research Foundation Synthetic peptides and peptide mimetics

Also Published As

Publication number Publication date
AU5435598A (en) 1998-06-03

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