WO1998020899A9 - Mutant de serpulina hyodysenteriae a virulence attenuee utile dans la prophylaxie de la dysenterie porcine - Google Patents
Mutant de serpulina hyodysenteriae a virulence attenuee utile dans la prophylaxie de la dysenterie porcineInfo
- Publication number
- WO1998020899A9 WO1998020899A9 PCT/US1997/021199 US9721199W WO9820899A9 WO 1998020899 A9 WO1998020899 A9 WO 1998020899A9 US 9721199 W US9721199 W US 9721199W WO 9820899 A9 WO9820899 A9 WO 9820899A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hyodysenteriae
- vira
- mutant
- dna sequence
- serotype
- Prior art date
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Definitions
- the present invention relates to Serpulina hyodysenteriae. and more particularly, to the formulation of S. hyodysenteriae mutant strains through the specific disruption of a gene involved in virulence and the mutant strains so produced.
- the present invention also relates to a live cell vaccine for swine dysentery employing the S. hyodysenteriae mutant strains according to the present invention and a method of increasing the resistance against dysentery infection in animals by administering the vaccine according to the present invention.
- mice After exposure to CO 2 , the mice were immediately processed for necropsy, as previously described (Mysore and Duhamel, 1994; Zhang et al., 1995). Cecal gross changes were recorded and sections of ceca were collected for histopathologic and bacteriologic examinations. For histopathologic examination, transverse and longitudinal sections of the cecum from each mouse were placed in PLP fixative (1 mM sodium m-periodate, 75 mM lysine, and 2 percent paraformaldehyde in 37 mM phosphate buffer, pH 7.4; McLean and Nolsane, 1974), embedded in paraffin, sectioned at 5 ⁇ m and stained with hematoxylin and eosin.
- PLP fixative 1 mM sodium m-periodate, 75 mM lysine, and 2 percent paraformaldehyde in 37 mM phosphate buffer, pH 7.4; McLean and Nolsane, 1974
- the dosage regimen may include one or more administrations, with multiple inoculations being administered from 1 to about 14 days apart.
- the vaccine administration may be provided in conjunction with other antigens capable of eliciting an immune response, either by multiple administrations or by combination in a single formulation.
- antigens include killed cells of a strain of S. hyodysenteriae. such as a virulent strain, for example, B204 or B234.
- Other antigens include S. hyodysenteriae hemolysin, cytotoxin, outer membrane proteins and lipopolysaccharides, for example.
- Antigenic proteins may be harvested and/or purified from cell cultures or may be produced by recombinant techniques.
- ADDRESSEE SUITER AND ASSOCIATES
- PC B
- STREET 11516 NICHOLAS STREET
Abstract
On a produit des mutants de Serpulina hyodysenteriae à virulence atténuée en éliminant spécifiquement un gène déterminé comme étant impliqué dans la virulene de S. hyodysenteriae. Les souches de mutants atténués de S. hyodysenteriae ainsi produites sont utiles en tant que vaccins atténués vivants permettant d'accroître la résistance des porcs à l'infection par la dysenterie.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU53592/98A AU5359298A (en) | 1996-11-12 | 1997-11-12 | Attenuated (serpulina hyodysenteriae) mutant for prevention of swine dysentery |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US3066296P | 1996-11-12 | 1996-11-12 | |
| US60/030,662 | 1996-11-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1998020899A1 WO1998020899A1 (fr) | 1998-05-22 |
| WO1998020899A9 true WO1998020899A9 (fr) | 1998-08-27 |
Family
ID=21855333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/021199 WO1998020899A1 (fr) | 1996-11-12 | 1997-11-12 | Mutant de serpulina hyodysenteriae a virulence attenuee utile dans la prophylaxie de la dysenterie porcine |
Country Status (2)
| Country | Link |
|---|---|
| AU (1) | AU5359298A (fr) |
| WO (1) | WO1998020899A1 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010054437A1 (fr) * | 2008-11-14 | 2010-05-20 | Spirogene Pty Ltd | Souches de vaccin de brachyspira hyodysenteriae |
| WO2014096013A1 (fr) | 2012-12-21 | 2014-06-26 | Boehringer Ingelheim Vetmedica Gmbh | Nouvelles protéines membranaires externes recombinées issues de brachyspira hyodysenteriae et leurs utilisations |
| RU2018127200A (ru) | 2016-01-07 | 2020-02-07 | Университейт Гент | Вакцинные штаммы brachyspira hyodysenteriae |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL103530A0 (en) * | 1991-10-25 | 1993-03-15 | Duphar Int Res | Treponema hyodysenteriae vaccine |
| US5382425A (en) * | 1992-01-13 | 1995-01-17 | Syntro Corporation | Recombinant swinepox virus |
| US5698394A (en) * | 1994-06-01 | 1997-12-16 | Board Of Regents Of The University Of Nebraska | Nucleotide sequences and methods for detection of Serpulina hyodysenteriae |
-
1997
- 1997-11-12 WO PCT/US1997/021199 patent/WO1998020899A1/fr active Application Filing
- 1997-11-12 AU AU53592/98A patent/AU5359298A/en not_active Abandoned
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