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WO1998012321A1 - Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster - Google Patents

Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster Download PDF

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Publication number
WO1998012321A1
WO1998012321A1 PCT/GB1997/002059 GB9702059W WO9812321A1 WO 1998012321 A1 WO1998012321 A1 WO 1998012321A1 GB 9702059 W GB9702059 W GB 9702059W WO 9812321 A1 WO9812321 A1 WO 9812321A1
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WO
WIPO (PCT)
Prior art keywords
plant
plant cell
expression vector
domain
protein
Prior art date
Application number
PCT/GB1997/002059
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English (en)
Inventor
Peter Meyer
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The University Of Leeds
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of Leeds filed Critical The University Of Leeds
Priority to AU37036/97A priority Critical patent/AU3703697A/en
Priority to CA002265898A priority patent/CA2265898A1/fr
Priority to EP97933800A priority patent/EP0939811A1/fr
Publication of WO1998012321A1 publication Critical patent/WO1998012321A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43577—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies
    • C07K14/43581—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies from Drosophila
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • the invention relates to recombinantly engineered plant cells which provide for plants with improved vigour; plants including such plant cells; and seeds derived from such plants.
  • the chromosomes of higher eukaryotes are composed of euchromatin and heterochromatin.
  • Heterochromatin is distinguished from euchromatin in that it remains condensed through interphase, replicates late in the cell cycle, is enriched for repetitive sequences and is relatively poor in the number of genes.
  • HPI is a heterochromatin protein
  • Pc is part of a complex that maintains the repressed state of homeotic genes.
  • GFP green fluorescent protein
  • transgenic plants expressing the HPI chromo domain Moreover further experimentation with transgenic plants expressing the HPI chromo domain has indicated that the transgene may be able to protect plants from environmental stress.
  • HPI transgene has protective properties and enables seedlings to tolerate elevated levels of CdS0 4 in growth medium.
  • a plant cell including, in expressionable form, the gene, or part thereof, encoding at least the chromo domain of heterochromatin protein 1, or a gene, or part thereof, having greater than 60% homology therewith.
  • HPI and Pc are very similar they differ by approximately 18 amino acids representing a variation of approximately 60% homology (ie 27/45). Since the functional effectiveness of the two proteins, in terms of plant yield, is so distinct it follows that the variations are significant and that at least one of the differences between the two proteins confers a distinct advantage in terms of plant growth. Thus although the two proteins have 27 identical amino acids and five amino acids from the same group, this similarity is not sufficient to confer desirable growth promoting properties on both proteins. It therefore follows that a protein identical to HPI or having greater than 60% and ideally 70% homology therewith, or more ideally, greater than 70% homology therewith, can be used in the invention to advantageously affect plant growth.
  • said gene is derived from the genus Drosophila.
  • a construct comprising a gene, or part thereof, encoding at least the chromo domain of heterochromatin protein 1, or a gene, or part thereof, having greater than 60% homology therewith, coupled to a nuclear targeting domain so as to ensure that said HPI gene is imported into the nucleus of the target cell.
  • a plasmid or vector suitable for effecting transformation of a plant cell with the construct of the invention which vector or plasmid includes the construct of the invention.
  • a plant including at least one plant cell according to the invention.
  • seeds obtained from a plant including at least one plant cell according to the invention.
  • a method for improving plant productivity of a selected plant comprising transforming a plant cell from said selected plant with an agent including a gene, or part thereof, encoding at least the chromo domain of heterochromatin protein 1, or a gene, or part thereof, having greater than 60% homology therewith; and culturing said transformed plant cell, under suitable culture conditions, so as to provide for the growth of a corresponding plant.
  • said gene, or part thereof is further coupled to a nuclear targeting domain so as to ensure that the gene, or part thereof, is imported into the nucleus of the target cells.
  • Figure 1 shows the DNA sequence structure and corresponding amino acid sequence structure of the chromo domain of the heterochromatin protein 1 and the chromo domain is represented by sequence 603-738.
  • Figure IB shows the amino acid sequence structure of the chromo domains of the Drosophila polycomb protein and heterochromatin protein 1;
  • Figure 2 shows the design of the protein fusion construct used in the methods described herein;
  • Figure 3 illustrates location of the fusion protein in the nucleus of a target cell
  • Figure 4A shows improved vigour of expressing line 855/3 compared to wide type SRI
  • Figure 4B shows improved vigour of strong expressing line 855/3-4 compared to the weak expressing line 873/2-3;
  • Figure 5 shows improved growth under salt stress conditions
  • Figure 6 shows improved growth of various strains either expressing or not expressing the transgene encoding the chromo domain of heterochromatin protein 1 ;
  • Figure 7 shows the vector used for the transfer of the chromo domains of polycomb and heterochromatin protein 1 into plant cells
  • Table 2 shows improved biomass yield of seedlings expressing the HPI transgeneic (EXP) and non-expressing seedlings (NONEXP) cultured in 0.5 x Ms medium, 50 ⁇ M CdS0 4 .
  • Havana SRI (Maliga et al. 1973) grown on LS medium were used for protoplasts isolation.
  • the basal medium used for isolation and incubation of protoplasts was M3, a K3 medium (Nagy and Maliga 1976) supplemented with vitamins, organic acids, sugars and sugar alcohols and vitamin-free casamino acid according to Kao and Michayluk (1975).
  • leaf halves without midribs were soaked in M3 medium containing 0.4 M sucrose and cut into thin sections. 2 g of leaf material were incubated in the dark in 30 ml enzyme solution ( 1.3 % Cellulase "Onozuka R- 10" (Serva), Heidelberg, FRG), 0.55% Mazerozyme R-10 (Serva) in M3 medium 0.4 M sucrose) for 20-22 h at room temperature. After lh or moderate swirling the digest was filtered through at lOO ⁇ m mesh filter.
  • Protoplasts were floated by centrifugation at 100 g for 10 min and washed twice with osmoticum (0.16 M CaC , 0.5 % w/v 2(n-Morpholino)-ethanesul- phonic acid (MES), 250 mM mannitol, pH 5.6).
  • osmoticum 0.16 M CaC , 0.5 % w/v 2(n-Morpholino)-ethanesul- phonic acid (MES), 250 mM mannitol, pH 5.6).
  • the vector used to transfer the chromo domains of polycomb and heterochromatin protein 1 is shown in Figure 7.
  • the vector is referenced 35shgfp.
  • the restriction sites are clearly shown.
  • the vector is based on an EcoRI-Sall fragment from pBR322 containing the bacterial origin of replication and the amp resistance gene for bacterial transformation.
  • the plant selectable marker is a HPT cassette (gift from Dr David Wing and Dr Czaba Concz, Max-Planck-Institute for Breeding Research, Cologne, Germany) which contains a nopaline synthase promoter, a hygromycin resistance gene and a gene 4 polyadenylation region.
  • the GFP construct is inserted between a 35S promoter and terminator.
  • Protoplast transformation The fusion method of Hein et al. (1983) was adapted for DNA uptake. About 250,000 protoplasts were washed with osmoticum and resuspended in a final volume of 120 l osmoticum. 10-30 ⁇ g of DNA dissolved in 20 ⁇ l sterile water were added. ⁇ A0u ⁇ PEG solution (0.5% N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid (Hepes), 25% PEG 6000, 0.45 M Mannitol, 0.1M Ca(N0 3 ) 2 pH 9.5) were added slowly.
  • Hepes N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
  • Protoplasts were cultured using the bead type technique of Shillito et al. (1983) which was modified as follows: one week after transformation 3 ml protoplast solution was mixed with 3 ml M3 (0.3 M sucrose, 1 mg/1 NAA, 0.2 mg/ 1 kinetin) containing 1 %
  • Seedlings were derived from crosses of HPI transgeneic lines with SP1 wild- type and grown on MS medium containing 150 mM NaCl or 0.5 x MS medium containing 120 mM NaCl. Non-transgeneic controlled plants were grown on corresponding MS medium but lacking NaCl. Seedling growth was monitored periodically at 11 days post sowing, 19 days post sowing and 24 days post sowing as indicated in Figure 5.
  • the protein to be tested in this case either the chromo domain of heterochromatin protein 1 or polycomb was linked to a nuclear targeting domain, so as to ensure that the fusion construct was targeted to the nucleus of the plant cell, and also a green fluorescent protein to allow visualisation of the transgene product within a transformed cell.
  • Transformants were self pollinated and seeds were germinated in the greenhouse and after 5 weeks transformed plants whose cells contained the fusion construct were compared with wide type plants.
  • FIG. 4B Shown in Figure 4B is a comparison of what we have termed our strong expressing cell line 855/3 with what we have termed a weak expressing cell line 873/2-3. It can be seen that the strongly expressing cell line shows more growth than the weakly expressing cell line. Moreover, it can also be seen that both cell lines show improved vigour vis-a-vis the wide type.
  • salt stress and desertification is a major limitation for the use of soil in agriculture, indeed, each year 21 million hectares of farm land provide no economic return because of desertification.
  • HPI transgenic plant cell-line 855/3-6 was cultured in 0.5 x MS containing 50 mM CdS0 4 .
  • Table 2 represents two experiments showing the protective properties of HPI in the presence of CdS0 4 . Those seedlings expressing the HP l/GFP fusion protein show a 1.7 - 4 fold increase in biomass yield when compared to non-expressing controlled seedlings indicating that transgene product expression has protective properties in the presence of cadmium.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une plante ou une cellule végétale contenant le domaine 'chromo' de la protéine hétérochromatine 1, idéalement dérivée du genre Drosophila, ledit domaine conférant un avantage sélectif à ladite plante ou à ladite cellule.
PCT/GB1997/002059 1996-09-20 1997-08-01 Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster WO1998012321A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU37036/97A AU3703697A (en) 1996-09-20 1997-08-01 Recombinant plant cells expressing heterochromatin 1 (HP1) of drosophila melan ogaster
CA002265898A CA2265898A1 (fr) 1996-09-20 1997-08-01 Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster
EP97933800A EP0939811A1 (fr) 1996-09-20 1997-08-01 Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9619633.2A GB9619633D0 (en) 1996-09-20 1996-09-20 Recombinant plant cells
GB9619633.2 1996-09-20

Publications (1)

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WO1998012321A1 true WO1998012321A1 (fr) 1998-03-26

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PCT/GB1997/002059 WO1998012321A1 (fr) 1996-09-20 1997-08-01 Cellules vegetales recombinees exprimant la proteine heterochromatine 1 (hp1) du drosophila melanogaster

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EP (1) EP0939811A1 (fr)
CN (1) CN1230992A (fr)
AU (1) AU3703697A (fr)
CA (1) CA2265898A1 (fr)
GB (1) GB9619633D0 (fr)
WO (1) WO1998012321A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001047975A1 (fr) * 1999-12-27 2001-07-05 Shanghai Biowindow Gene Development Inc. Nouveau polypeptide, proteine 10 contenant un domaine chromo, et polynucleotide codant pour ce polypeptide
WO2001070784A1 (fr) * 2000-03-07 2001-09-27 Biowindow Gene Development Inc. Shanghai Nouveau polypeptide, proteine humaine 17 contenant un domaine de structure chromo, et polynucleotide codant pour ce polypeptide

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006651A1 (fr) * 1989-10-31 1991-05-16 Queen's University At Kingston Gene conferant a des cellules la capacite de tolerer les sels
WO1995025787A1 (fr) * 1994-03-24 1995-09-28 Rutgers University Procede engendrant la sterilite male cytoplasmique dans des vegetaux et son emploi pour la production de semences hybrides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006651A1 (fr) * 1989-10-31 1991-05-16 Queen's University At Kingston Gene conferant a des cellules la capacite de tolerer les sels
WO1995025787A1 (fr) * 1994-03-24 1995-09-28 Rutgers University Procede engendrant la sterilite male cytoplasmique dans des vegetaux et son emploi pour la production de semences hybrides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MCCUE K F ET AL: "DROUGHT AND SALT TOLERANCE: TOWARDS UNDERSTANDING AND APPLICATION", TRENDS IN BIOTECHNOLOGY, vol. 8, December 1990 (1990-12-01), pages 358 - 362, XP002034432 *
PLATERO J. ET AL.: "Functional analysis of the chromo domain of HP1", THE EMBO JOURNAL, vol. 14, no. 16, - 15 August 1995 (1995-08-15), pages 3977 - 3986, XP002047784 *
SINGH P. ET AL.: "A sequence motif found in Drosophila heterochromatin protein is conserved in animals and plants", NUCLEIC ACIDS RESEARCH, vol. 19, no. 4, 25 February 1991 (1991-02-25), pages 789 - 794, XP002047785 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001047975A1 (fr) * 1999-12-27 2001-07-05 Shanghai Biowindow Gene Development Inc. Nouveau polypeptide, proteine 10 contenant un domaine chromo, et polynucleotide codant pour ce polypeptide
WO2001070784A1 (fr) * 2000-03-07 2001-09-27 Biowindow Gene Development Inc. Shanghai Nouveau polypeptide, proteine humaine 17 contenant un domaine de structure chromo, et polynucleotide codant pour ce polypeptide

Also Published As

Publication number Publication date
EP0939811A1 (fr) 1999-09-08
GB9619633D0 (en) 1996-11-06
CA2265898A1 (fr) 1998-03-26
CN1230992A (zh) 1999-10-06
AU3703697A (en) 1998-04-14

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