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WO1998008954A3 - Production of heme and recombinant hemoproteins - Google Patents

Production of heme and recombinant hemoproteins Download PDF

Info

Publication number
WO1998008954A3
WO1998008954A3 PCT/US1997/014165 US9714165W WO9808954A3 WO 1998008954 A3 WO1998008954 A3 WO 1998008954A3 US 9714165 W US9714165 W US 9714165W WO 9808954 A3 WO9808954 A3 WO 9808954A3
Authority
WO
WIPO (PCT)
Prior art keywords
heme
production
methods
hemoproteins
recombinant
Prior art date
Application number
PCT/US1997/014165
Other languages
French (fr)
Other versions
WO1998008954A2 (en
Inventor
Elaine A Best
Evie L Verderber
Louise J Lucast
Original Assignee
Somatogen Inc
Elaine A Best
Evie L Verderber
Louise J Lucast
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Somatogen Inc, Elaine A Best, Evie L Verderber, Louise J Lucast filed Critical Somatogen Inc
Priority to AU41495/97A priority Critical patent/AU4149597A/en
Publication of WO1998008954A2 publication Critical patent/WO1998008954A2/en
Publication of WO1998008954A3 publication Critical patent/WO1998008954A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to methods of enhancing the production of heme-containing proteins by increasing the accumulation of heme in heterologous host cells. The methods are accomplished by exposing the host cells to increased amounts of endogenous or exogenous δ-aminolevulinic acid (ALA) resulting in an increase in heme production. Enhancing the endogenous production of ALA can be accomplished by adding multiple copies of the hemA gene into the host cell. Methods of producing enhanced amounts of heme are also provided.
PCT/US1997/014165 1996-08-30 1997-08-29 Production of heme and recombinant hemoproteins WO1998008954A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU41495/97A AU4149597A (en) 1996-08-30 1997-08-29 Production of heme and recombinant hemoproteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US2581296P 1996-08-30 1996-08-30
US60/025,812 1996-08-30

Publications (2)

Publication Number Publication Date
WO1998008954A2 WO1998008954A2 (en) 1998-03-05
WO1998008954A3 true WO1998008954A3 (en) 1998-11-26

Family

ID=21828188

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/014165 WO1998008954A2 (en) 1996-08-30 1997-08-29 Production of heme and recombinant hemoproteins

Country Status (2)

Country Link
AU (1) AU4149597A (en)
WO (1) WO1998008954A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102016116794A1 (en) * 2016-09-08 2018-03-08 Universität Bielefeld Process and composition for the preparation of aminolevulinic acid
WO2022269550A1 (en) * 2021-06-24 2022-12-29 Reliance Industries Limited Genetically modified microorganism, method(s) and application(s) thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992013076A1 (en) * 1991-01-10 1992-08-06 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active eukaryotic cytochrome p450s in bacteria
US5635375A (en) * 1995-01-09 1997-06-03 Regents Of The University Of Colorado Method of increasing the yield and heme saturation of cystathione β-synthase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992013076A1 (en) * 1991-01-10 1992-08-06 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active eukaryotic cytochrome p450s in bacteria
US5635375A (en) * 1995-01-09 1997-06-03 Regents Of The University Of Colorado Method of increasing the yield and heme saturation of cystathione β-synthase

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ANNUAL MEETING OF THE AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, SAN FRANCISCO, CALIFORNIA, USA, MAY 21-25, 1995. *
CHEN W ET AL: "5- Aminolevulinic acid synthesis in Escherichia coli requires expression of hemA.", JOURNAL OF BACTERIOLOGY, (1994 MAY) 176 (9) 2743-6., XP002054090 *
CHEN W ET AL: "Over-expression of glutamyl -tRNA reductase in Escherichia coli.", FASEB JOURNAL 9 (6). PAGE A1352. ABSTRACT 559, 24 April 1995 (1995-04-24), XP002054089 *
DARIE S ET AL: "Effect of heme and oxygen availability on hemA gene expression in Escherichia coli: role of the fnr, arcA, and himA gene products.", JOURNAL OF BACTERIOLOGY, (1994 SEP) 176 (17) 5270-6., XP002054092 *
HARRIS III, W. ET AL.: "Enhancement of bacterial porphyrin biosynthesis by exogenous aminolevulinic acid and isomer specificity of the products.", BIOORGANIC CHEMISTRY, vol. 21, 1993, pages 209 - 220, XP002054093 *
LOOKER D ET AL: "A HUMAN RECOMBINANT HAEMOGLOBIN DESIGNED FOR USE AS A BLOOD SUBSTITUTE", NATURE, vol. 356, no. 6366, 19 March 1992 (1992-03-19), pages 258 - 260, XP000606891 *
VERDERBER E ET AL: "Role of the hemA gene product and delta- aminolevulinic acid in regulation of Escherichia coli heme synthesis.", JOURNAL OF BACTERIOLOGY, (1997 JUL) 179 (14) 4583-90., XP002054094 *
VERKAMP E ET AL: "Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene.", JOURNAL OF BACTERIOLOGY, (1989 SEP) 171 (9) 4728-35., XP002054091 *

Also Published As

Publication number Publication date
WO1998008954A2 (en) 1998-03-05
AU4149597A (en) 1998-03-19

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