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WO1998007867A3 - Metabolically engineered lactic acid bacteria and means for providing same - Google Patents

Metabolically engineered lactic acid bacteria and means for providing same Download PDF

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Publication number
WO1998007867A3
WO1998007867A3 PCT/DK1997/000336 DK9700336W WO9807867A3 WO 1998007867 A3 WO1998007867 A3 WO 1998007867A3 DK 9700336 W DK9700336 W DK 9700336W WO 9807867 A3 WO9807867 A3 WO 9807867A3
Authority
WO
WIPO (PCT)
Prior art keywords
lactic acid
genes
acid bacteria
metabolically engineered
manufacturing
Prior art date
Application number
PCT/DK1997/000336
Other languages
French (fr)
Other versions
WO1998007867A2 (en
Inventor
Jose Arnau
Hans Israelsen
Astrid Vrang
Flemming Joergensen
Soeren Michael Madsen
Original Assignee
Bioteknologosk Inst
Jose Arnau
Hans Israelsen
Astrid Vrang
Flemming Joergensen
Soeren Michael Madsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioteknologosk Inst, Jose Arnau, Hans Israelsen, Astrid Vrang, Flemming Joergensen, Soeren Michael Madsen filed Critical Bioteknologosk Inst
Priority to EP97934442A priority Critical patent/EP0938566A2/en
Priority to NZ334294A priority patent/NZ334294A/en
Priority to CA002262418A priority patent/CA2262418A1/en
Priority to AU37659/97A priority patent/AU721803B2/en
Publication of WO1998007867A2 publication Critical patent/WO1998007867A2/en
Publication of WO1998007867A3 publication Critical patent/WO1998007867A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The complete DNA sequences for adhE and pf1 genes of Lactococcus lactis, recombinant replicons comprising one or both of these genes or comprising mutants or variants hereof including mutants in which the genes are inactivated, and recombinant lactic acid bacteria comprising such a replicon are provided. The gene sequences and/or sequences regulating the expression of the genes can be modified to provide metabolically engineered lactic acid bacteria which have an enhanced or reduced production of one or more metabolites resulting from citrate and/or sugar fermentation. Such metabolically modified cells are useful as starter cultures in the manufacturing of food products and animal feed having improved flavour and/or shelf life, including dairy products, or they can be used directly in the manufacturing of a lactic acid bacterial metabolite.
PCT/DK1997/000336 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same WO1998007867A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP97934442A EP0938566A2 (en) 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same
NZ334294A NZ334294A (en) 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same
CA002262418A CA2262418A1 (en) 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same
AU37659/97A AU721803B2 (en) 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70145896A 1996-08-22 1996-08-22
US08/701,458 1996-08-22

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US98109797A A-371-Of-International 1997-12-17 1997-12-17
US10/267,989 Continuation US20030199035A1 (en) 1997-12-17 2002-10-10 Metabolically engineered lactic acid bacteria and means for providing same

Publications (2)

Publication Number Publication Date
WO1998007867A2 WO1998007867A2 (en) 1998-02-26
WO1998007867A3 true WO1998007867A3 (en) 1998-05-22

Family

ID=24817464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK1997/000336 WO1998007867A2 (en) 1996-08-22 1997-08-20 Metabolically engineered lactic acid bacteria and means for providing same

Country Status (5)

Country Link
EP (1) EP0938566A2 (en)
AU (1) AU721803B2 (en)
CA (1) CA2262418A1 (en)
NZ (1) NZ334294A (en)
WO (1) WO1998007867A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9724627D0 (en) 1997-11-20 1998-01-21 Genencor Int Bv Gram positive microorganism formate pathway
KR20160013069A (en) * 2013-05-10 2016-02-03 바이오위시 테크놀로지스, 인크. Compositions comprising a mixture of bacteria comprising pediococcus and lactobacillus and methods for decreasing the effects of alcohols
JP7012663B2 (en) * 2016-12-15 2022-02-14 株式会社カネカ New host cell and method for producing the target protein using it
CN112601808B (en) * 2018-08-10 2025-05-02 协和发酵生化株式会社 Microorganism producing eicosapentaenoic acid and method for producing eicosapentaenoic acid
CN111471660B (en) * 2020-03-12 2023-11-24 广州辉园苑医药科技有限公司 Acetaldehyde dehydrogenase recombinant gene, lactic acid bacteria carrier and application thereof

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DOROTHEA KESSLER ET AL.: "Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE", FEBS LETTERS, vol. 281, no. 1,2, 9 April 1991 (1991-04-09), AMSTERDAM NL, pages 59 - 63, XP002045430 *
GERHARD WEIDNER & GARY SAWERS: "Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme of Clostridium pasteurianum", JOURNAL OF BACTERIOLOGY, vol. 178, no. 8, April 1996 (1996-04-01), pages 2440 - 2444, XP002055590 *
JOACHIM KNAPPE ET AL.: "A radical-chemical route to acetyl-CoA: the anaerobically induced pyruvate formate-lyase system of Escherichia coli", FEMS MICROBIOLOGY REVIEWS, vol. 75, no. 4, August 1990 (1990-08-01), pages 383 - 398, XP002045432 *
JOSÉ ARNAU ET AL.: "Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase", JOURNAL OF BACTERIOLOGY, vol. 179, no. 18, September 1997 (1997-09-01), pages 5884 - 5891, XP002055591 *
RAMESH V. NAIR ET AL.: "Molecular characterization of an aldehyde/alcohol dehydroganase gene from Clostridium acetobutylicum ATCC 824", JOURNAL OF BACTERIOLOGY, vol. 176, no. 3, February 1994 (1994-02-01), pages 871 - 885, XP002045431 *
VALERIE M. MARSHALL ET AL: "Threonine aldolase and alcohol dehydrogenase activities in Lactobacillus bulgaricus and Lactobacillus acidophilus and their contribution to flavour production in fermented milks", JOURNAL DAIRY RESEARCH, vol. 50, no. 3, 1983, pages 375 - 379, XP000677656 *
WENGANG YANG ET AL: "Entamoeba histolytica has an alcohol dehydrogenase homologous to the multifunctional adhE gene product of Escherichia coli", MOLECULAR AND BIOCHEMICAL PARASITOLOGY, vol. 64, no. 2, 1994, pages 253 - 260, XP000646353 *
YASUHITO YAMAMOTO ET AL.: "Cloning and sequence analysis of the pfl gene encoding pyruvate formate-lyase from Streptococcus mutans", INFECTION AND IMMUNITY, vol. 64, no. 2, February 1996 (1996-02-01), WASHINGTON US, pages 385 - 391, XP002045550 *
YU-MEI CHEN ET AL.: "Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 173, no. 24, December 1991 (1991-12-01), pages 8009 - 8013, XP002045433 *

Also Published As

Publication number Publication date
CA2262418A1 (en) 1998-02-26
WO1998007867A2 (en) 1998-02-26
NZ334294A (en) 2000-02-28
AU3765997A (en) 1998-03-06
EP0938566A2 (en) 1999-09-01
AU721803B2 (en) 2000-07-13

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