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WO1998006707A1 - Carbamates et isoxazolidines cycliques antagonistes de l'iib/iiia - Google Patents

Carbamates et isoxazolidines cycliques antagonistes de l'iib/iiia Download PDF

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Publication number
WO1998006707A1
WO1998006707A1 PCT/US1997/013761 US9713761W WO9806707A1 WO 1998006707 A1 WO1998006707 A1 WO 1998006707A1 US 9713761 W US9713761 W US 9713761W WO 9806707 A1 WO9806707 A1 WO 9806707A1
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Prior art keywords
substituted
alkyl
acetyl
cis
phenyl
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PCT/US1997/013761
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English (en)
Inventor
Fuqiang Jin
Pasquale Nicholas Confalone
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Dupont Pharmaceuticals Company
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Publication date
Application filed by Dupont Pharmaceuticals Company filed Critical Dupont Pharmaceuticals Company
Priority to AU39706/97A priority Critical patent/AU3970697A/en
Priority to CA002263311A priority patent/CA2263311A1/fr
Priority to EP97937112A priority patent/EP0922039A1/fr
Priority to JP10509820A priority patent/JP2000516230A/ja
Publication of WO1998006707A1 publication Critical patent/WO1998006707A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D261/00—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings
    • C07D261/02—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/04—1,3-Oxazines; Hydrogenated 1,3-oxazines
    • C07D265/06—1,3-Oxazines; Hydrogenated 1,3-oxazines not condensed with other rings
    • C07D265/08—1,3-Oxazines; Hydrogenated 1,3-oxazines not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D265/10—1,3-Oxazines; Hydrogenated 1,3-oxazines not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with oxygen atoms directly attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • the present invention relates generally to cyclic carbamates and isoxazolidines which are useful as antagonists of the platelet glycoprotein Ilb/IIIa fibrinogen receptor complex, to pharmaceutical compositions containing such compounds, processes for preparing such compounds, and to methods of using these compounds for the inhibition of platelet aggregation, as thrombolytics, and/or for the treatment of thromboembolic disorders .
  • Hemostasis is the normal physiological process in which bleeding from an injured blood vessel is arrested. It is a dynamic and complex process in which platelets play a key role. Within seconds of vessel injury, resting platelets become activated and are bound to the exposed matrix of the injured area by a phenomenon called platelet adhesion. Activated platelets also bind to each other in a process called platelet aggregation to form a platelet plug. The platelet plug can stop bleeding quickly, but it must be reinforced by fibrin for long-term effectiveness, until the vessel injury can be permanently repaired.
  • Thrombosis may be regarded as the pathological condition wherein improper activity of the hemostatic mechanism results in intravascular thrombus formation.
  • Activation of platelets and the resulting platelet aggregation and platelet factor secretion has been associated with a variety of pathophysiological conditions including cardiovascular and cerebrovascular thromboembolic disorders, for example, the romboembolic disorders associated with unstable angina, myocardial infarction, transient lschemic attack, stroke, atherosclerosis and diabetes.
  • the contribution of platelets to these disease processes stems from their ability to form aggregates, or platelet thrombi, especially in the arterial wall following injury.
  • Platelets are activated by a wide variety of agonists resulting in platelet shape change, secretion of granular contents and aggregation. Aggregation of platelets serves to further focus clot formation by concentrating activated clotting factors at the site of injury.
  • endogenous agonists including adenosine diphosphate (ADP) , serotonin, arachidonic acid, thrombin, and collagen, have been identified. Because of the involvement of several endogenous agonists in activating platelet function and aggregation, an inhibitor which acts against all agonists would represent a more efficacious antiplatelet agent than currently available antiplatelet drugs, which are agonist-specific.
  • Current antiplatelet drugs are effective against only one type of agonist; these include aspirin, which acts against arachidonic acid; ticlopidme, which acts against ADP; thromboxane A ? synthetase inhibitors or receptor antagonists, which act against thromboxane A 2 ; and hirudm, which acts against thrombin.
  • GPIIb/IIIa platelet glycoprotei ⁇ Ilb/IIIa complex
  • GPIIb/IIIa platelet glycoprotei ⁇ Ilb/IIIa complex
  • a recent review of GPIIb/IIIa is provided by Phillips et al. Cell (1991) 65: 359-362.
  • the development of a GPIIb/IIIa antagonist represents a promising new approach for antiplatelet therapy.
  • GPIIb/IIIa does not bind soluble proteins on unstimulated platelets, but GPIIb/IIIa in activated platelets is known to bind four soluble adhesive proteins, namely fibrinogen, von Willebrand factor, fibronectin, and vitronectin.
  • the binding of fibrinogen and von Willebrand factor to GPIIb/IIIa causes platelets to aggregate.
  • the binding of fibrinogen is mediated in part by the Arg-Gly-Asp (RGD) recognition sequence which is common to the adhesive proteins that bind GPIIb/IIIa.
  • European Patent Application Publication Number 478328 relates to compounds having the general formula:
  • European Patent Application Publication Nu ber 525629 (corresponds to Canadian Patent Application Publication Number 2,074,685) discloses compounds having the general formula:
  • PCT Patent Application 9307867 relates to compounds having the general formula:
  • One aspect of this invention provides novel compounds of Formula I (described below) which are useful as antagonists of the platelet glycoprotem Ilb/Ilia complex.
  • the compounds of the present invention inhibit the binding of fibrinogen to platelet glycoprotein Ilb/IIIa complex and inhibit the aggregation of platelets.
  • the present invention also includes pharmaceutical compositions containing such compounds of Formula I, and methods of using such compounds for the inhibition of platelet aggregation, as thrombolytics, and/or for the treatment of thromboembolic disorders.
  • the present invention also includes methods of treating cardiovascular disease, thrombosis or harmful platelet aggregation, reocclusion following thrombolysis, reperfusion injury, or restenosis by administering a compound of Formula I alone or in combination with one or more additional therapeutic agents selected from: anti-coagulants such as warfarin or hepa ⁇ n; anti-platelet agents such as aspirin, piroxicam or ticlopidine; thrombin inhibitors such as boroarginme derivatives, hirudm or argatroban; or thrombolytic agents such as tissue plasmmogen activator, anistreplase, urokinase or streptokmase; or combinations thereof.
  • anti-coagulants such as warfarin or hepa ⁇ n
  • anti-platelet agents such as aspirin, piroxicam or ticlopidine
  • thrombin inhibitors such as boroarginme derivatives, hirudm or argatroban
  • thrombolytic agents such as tissue plasmmogen activator
  • kits comprising one or more containers containing pharmaceutical dosage units comprising a compound of Formula I, for the treatment of cell adhesion related disorders, including but not limited to thromboembolic disorders .
  • Z is selected from a bond (i.e. is absent), O, S, or
  • R 2 is selected from H, aryl (Ci-Cio alkoxy) carbonyl, or Ci-Cio alkoxycarbonyl, C 1 -C 4 alkyl, C 3 -C 6 alkenyl;
  • R 3 is selected from H, C1-C 6 alkyl, C 2 -C 6 alkenyl, C -C 6 alkynyl, C3-C7 cycloalkyl, - (phenyl) - substituted with 0-2 R 6a , or - (py ⁇ dyl) - substituted with 0- 2R 6 ;
  • R is selected from H or C1-C 1 0 alkyl substituted with 0-1 R 4b ;
  • R 4b is selected from Ci-C ⁇ alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C 3 -C7 cycloalkyl, C 7 -C 14 bicycloalkyl,
  • X is selected from -C(O)- or a single bond, i.e. X is absent; Y is selected from hydroxy, C to Cio alkyloxy, C 3 to Cn cycloalkyloxy, Q to Cio aryloxy, C 7 to Cn aralkyloxy, C 3 to Cio alkylcarbonyloxyalkyloxy, C 3 to Cio alkoxycarbonyloxyalkyloxy, C 2 to Cio alkoxycarbonylalkyloxy, C5 to C o cycloalkylcarbonyloxyalkyloxy, C 5 to Cio cycloalkoxycarbonyloxyalkyloxy, C 5 to Cio cycloalkoxycarbonylalkyloxy, C 7 to Cn aryloxycarbonylalkyloxy, C % to C 12 aryloxycarbonyloxyalkyloxy, Cg to C 1 2 arylcarbonyloxyalkyloxy, C 5 to Cio alkoxyalkyl
  • R 9 and R 10 are each independently selected from H, C -C ⁇ alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, C 3 -C 7 cycloalkyl, - (phenyl) - substituted with 0-2 R 6a , or - (pyridyl) - substituted with 0-2R 6a ; R 11 is
  • R 12 and R 13 are each independently selected from H, Ci-Cio alkyl, C -Cio alkoxycarbonyl, Ci-Cio alkylcarbonyl, C -Cio alkylsulfonyl, heteroaryl (C 1 -C 4 alkyl )sulfonyl, aryl(C ⁇ -C ⁇ o alkyl) sulfonyl, arylsulfonyl, aryl, heteroarylcarbonyl, heteroarylsulfonyl, or heteroarylalkylcarbonyl, wherein said aryls and heteroaryls are optionally substituted with 0-3 substituents selected from the group consisting of C 1 -C 4 alkyl, C 1 -C 4 alkoxy, halo, CF 3 , and N0 2 ;
  • heterocyclic ring system having 1-3 heteroatoms selected independently from 0,S, and N, said heterocyclic ring being substituted with 0-4
  • C1-C6 alkyl substituted with a 5-10 membered heterocyclic ring system having 1-3 heteroatoms selected independently from 0,S, and N, said heterocyclic ring being substituted with 0-4R15;
  • R 14b 1S R 14a or H
  • R 15 is H, halogen, CF 3 , CN, N0 2 , NR 12 R 13 , C ⁇ -C 8 alkyl, C 2 - C 6 alkenyl, C 3 -C 11 cycloalkyl, C 4 -C 11 cycloalkylakyl, aryl, aryl(C ⁇ -C 6 alkyl)-, C 1 -C 6 alkoxy, or C 1 -C4 alkoxycarbonyl; provided that m and n are chosen such that the number of atoms connecting R 1 and Y is m the range of 10-18.
  • Preferred compounds of the present invention are compounds wherein:
  • R 2 is selected from H, Ci-Cio alkoxycarbonyl, or C 1 -C 4 alkyl;
  • R 3 is selected from H, C1-C6 alkyl, or - (phenyl) - substituted with 0-2R 6 ;
  • R 5 is selected from H or C 1 -C 4 alkyl
  • Ar is -(phenyl)- substituted with 0-2 R 5a
  • R 6a is selected from C 1 -C 4 alkyl, C 1 -C 4 alkoxy, halo, CF 3 , NO 2 , Or NR 12 R 13
  • X is selected from -C(O)- or a single bond, i.e. X is absent
  • Y is selected from: hydroxy;
  • Ci Ci to Cio alkoxy; methylcarbonyloxymethoxy- ; ethylcarbonyloxymethoxy- ; t-butylcarbonyloxymethoxy- ; cyclohexylcarbonyloxymethoxy- ; 1- (methylcarbonyloxy) ethoxy- ; 1- (ethylcarbonyloxy) ethoxy-;
  • R 9 and R 10 each are H
  • R 12 is selected from H, Ci-C ⁇ alkyl, C 1 -C 4 alkoxycarbonyl, Ci-Ce alkylcarbonyl, C ⁇ -C 6 alkylsulfonyl, aryl(C ⁇ -C 4 alkyl) sulfonyl, heteroaryl (C1-C4 alkyl) sulfonyl, arylsulfonyl, heteroarylsulfonyl, aryl, pyridylcarbonyl or pyridylmethylcarbonyl , wherein said aryls and heteroaryls are optionally substituted with 0-3 substituents selected from the group consisting of: C1-C4 alkyl, C1-C4 alkoxy, halo, CF 3 , and N0 2 ; and
  • R 13 is H.
  • thromboembolic disorders as used herein includes conditions involving platelet activation and aggregation, such as arterial or venous cardiovascular or cerebrovascular thromboembolic disorders, including, for example, thrombosis, unstable angina, first or recurrent myocardial infarction, lschemic sudden death, transient ischemic attack, stroke, atherosclerosis, venous thrombosis, deep vein thrombosis, thrombophlebitis, arterial embolism, coronary and cerebral arterial thrombosis, myocardial infarction, cerebral embolism, kidney embolisms, pulmonary embolisms, or such disorders associated with diabetes, comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Formula I described above.
  • the compounds of the present invention are useful for inhibiting the binding of fibrinogen to blood platelets, inhibiting aggregation of blood platelets, treating thrombus formation or embolus formation, or preventing thrombus or embolus formation in a mammal.
  • the compounds of the invention may be used as a medicament for blocking fibrinogen from acting at its receptor site in a mammal.
  • Compounds of the invention may be administered to patients where prevention of thrombosis by inhibiting binding of fibrinogen to the platelet membrane glycoprotein complex Ilb/IIIa receptor is desired. They are useful in surgery on peripheral arteries (arterial grafts, carotid endarterectomy) and in cardiovascular surgery where manipulation of arteries and organs, and/or the interaction of platelets with artificial surfaces, leads to platelet aggregation and consumption, and where the aggregated platelets may form thrombi and thromboemboli.
  • the compounds of the present invention may be administered to these surgical patients to prevent the formation of thrombi and thromboemboli.
  • Extracorporeal circulation is routinely used during cardiovascular surgery in order to oxygenate blood. Platelets adhere to surfaces of the extracorporeal circuit.
  • Adhesion is dependent on the interaction between GPIIb/IIIa on the platelet membranes and fibrinogen adsorbed to the surface of the extracorporeal circuit. Platelets released from artificial surfaces show impaired homeostatic function.
  • the compounds of the invention may be administered to prevent such ex vivo adhesion.
  • the compounds of the present invention may be used for other ex vivo applications to prevent cellular adhesion in biological samples.
  • Other applications of these compounds include prevention of platelet thrombosis, thromboembolism, and reocclusion during and after thrombolytic therapy and prevention of platelet thrombosis, thromboembolism and reocclusion after angioplasty of coronary and other arteries and after coronary artery bypass procedures .
  • the compounds of the present invention may also be used to prevent myocardial infarction.
  • the compounds of the present invention are useful as thrombolytics for the treatment of thromboembolic disorders.
  • the compounds of the present invention can also be administered in combination with one or more additional therapeutic agents select from: anti-coagulant or coagulation inhibitory agents, such as heparin or warfarin; anti-platelet or platelet inhibitory agents, such as aspirin, piroxicam, or ticlopidine; thrombin inhibitors such as boropeptides, hirudin or argatroban; or thrombolytic or fibrinolytic agents, such as plasminogen activators, anistreplase, urokinase, or streptokinase.
  • anti-coagulant or coagulation inhibitory agents such as heparin or warfarin
  • anti-platelet or platelet inhibitory agents such as aspirin, piroxicam, or ticlopidine
  • thrombin inhibitors such as boropeptides, hirudin or argatroban
  • thrombolytic or fibrinolytic agents such as plasminogen activators, anistreplase, urokinase,
  • the compounds of Formula I of the present invention can be administered in combination with one or more of the foregoing additional therapeutic agents, thereby to reduce the doses of each drug required to achieve the desired therapeutic effect.
  • the combination treatment of the present invention permits the use of lower doses of each component, with reduced adverse, toxic effects of each component.
  • a lower dosage minimizes the potential of side effects of the compounds, thereby providing an increased margin of safety relative to the margin of safety for each component when used as a single agent.
  • Such combination therapies may be employed to achieve synergistic or additive therapeutic effects for the treatment of thromboembolic disorders.
  • terapéuticaally effective amount it is meant an amount of a compound of Formula I that when administered alone or in combination with an additional therapeutic agent to a cell or mammal is effective to prevent or ameliorate the thromboembolic disease condition or the progression of the disease.
  • administered combination or “combination therapy” it is meant that the compound of Formula I and one or more additional therapeutic agents are administered concurrently to the mammal being treated.
  • each component may be administered at the same time or sequentially m any order at different points in time. Thus, each component may be administered separately but sufficiently closely in time so as to provide the desired therapeutic effect.
  • anti-coagulant agents or coagulation inhibitory agents
  • agents that inhibit blood coagulation include warfarin (available as CoumadinTM) and heparm.
  • anti-platelet agents denotes agents that inhibit platelet function such as by inhibiting the aggregation, adhesion or granular secretion of platelets.
  • agents include the various known non-steroidal anti-inflammatory drugs (NSAIDS) such as aspirin, lbuprofen, naproxen, sulindac, mdomethacin, mefenamate, droxicam, diclofenac, sulfinpyrazone, and piroxicam, including pharmaceutically acceptable salts or prodrugs thereof.
  • NSAIDS non-steroidal anti-inflammatory drugs
  • aspirin acetylsalicyclic acid or ASA
  • piroxicam acetylsalicyclic acid
  • Piroxicam is commercially available from Pfizer Inc. (New York, NY) , as FeldaneTM.
  • Other suitable antiplatelet agents include ticlopidine, including pharmaceutically acceptable salts or prodrugs thereof. Ticlopidine is also a preferred compound since it is known to be gentle on the gastro-mtestinal tract in use.
  • Still other suitable platelet inhibitory agents include thromboxane-A2-receptor antagonists and thromboxane-A2-synthetase inhibitors, as well as pharmaceutically acceptable salts or prodrugs thereof.
  • thrombin inhibitors or anti-thrombin agents, as used herein, denotes inhibitors of the se me protease thrombin and other inhibitors of
  • thrombin synthesis such as Factor XA.
  • various thrombin-mediated processes such as thrombin-mediated platelet activation (that is, for example, the aggregation of platelets, and/or the granular secretion of plasminogen activator inhibitor-1 and/or serotonin) and/or fibrin formation are disrupted.
  • Such inhibitors include boroarginine derivatives and boropeptides, hirudin and argatroban, including pharmaceutically acceptable salts and prodrugs thereof.
  • Boroarginine derivatives and boropeptides include
  • N-acetyl and peptide derivatives of boronic acid such as C-terminal ⁇ -aminoboronic acid derivatives of lysine, ornithine, arginine, homoarginine and corresponding isothiouronium analogs thereof.
  • hirudin includes suitable derivatives or analogs of hirudin, referred to herein as hirulogs, such as disulfatohirudin.
  • Boropeptide thrombin inhibitors include compounds described in Kettner et al., U.S. Patent No. 5,187,157 and European Patent Application Publication Number 293 881 A2 , the disclosures of which are hereby incorporated herein by reference.
  • boroarginine derivatives and boropeptide thrombin inhibitors include those disclosed in PCT Application Publication Number 92/07869 and European Patent Application Publication Number 471 651 A2, the disclosures of which are hereby incorporated herein by reference, in their entirety.
  • thrombolytics or fibrinolytic agents
  • fibrinolytics or fibrinolytics agents that lyse blood clots (thrombi) .
  • agents include tissue plasminogen activator, anistreplase, urokinase or streptokinase, including pharmaceutically acceptable salts or prodrugs thereof.
  • Tissue plasminogen activator (tPA) is commercially available from Genentech Inc., South San Francisco, California.
  • anistreplase refers to anisoylated plasminogen streptokinase
  • urokinase as used herein, is intended to denote both dual and single chain urokinase, the latter also being referred to herein as prourokmase.
  • Administration of the compounds of Formula I of the invention combination with such additional therapeutic agent may afford an efficacy advantage over the compounds and agents alone, and may do so while permitting the use of lower doses of each.
  • a lower dosage minimizes the potential of side effects, thereby providing an increased margin of safety.
  • GPIIb/IIIa is known to be overexpressed in metastatic tumor cells.
  • the compounds or combination products of the present invention may also be useful for the treatment, including prevention, of metastatic cancer.
  • the compounds of the present invention are also useful as standard or reference compounds, for example as a quality standard or control, in tests or assays involving the binding of fibrinogen to platelet GPIIb/IIIa.
  • Such compounds may be provided m a commercial kit, for example, for use in pharmaceutical research involving GPIIb/IIIa.
  • the compounds of the present invention may also be used in diagnostic assays involving platelet GPIIb/IIIa.
  • [ 8 invention that contain asymmetrically substituted carbon atoms may be isolated in optically active or racemic forms. It is well known in the art how to prepare optically active forms, such as by resolution of racemic forms or by synthesis, from optically active starting materials. All chiral, diastereome ⁇ c, racemic forms and all geometric lsomeric forms of a structure are intended, unless the specific stereochemistry or isomer form is specifically indicated.
  • any variable for example but not limited to, R 2 , R 4b , R 6a , R 12 ,and R 13 , n, etc.
  • its definition on each occurrence is independent of its definition at every other occurrence.
  • said group may optionally be substituted with up to two R 4 and R 4 at each occurrence is selected independently from the defined list of possible R 4 .
  • substituent when a substituent is listed without indicating the atom via which such substituent is bonded to the rest of the compound of Formula I, then such substituent may be bonded via any atom in such substituent.
  • substituent when the substituent is piperidinyl, or morpholmyl, unless specified otherwise, said piperidinyl or morphol yl, tetrazolyl group may be bonded to the rest of the compound of Formula I via any atom in such piperidinyl or morpholinyl, tetrazolyl group.
  • stable compound or stable structure it is meant herein a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.
  • substituted means that any one or more hydrogen on the designated atom is replaced with a selection from the indicated group, provided that the designated atom's normal valency is not exceeded, and that the substitution results m a stable compound.
  • 2 hydrogens on the atom are replaced.
  • alkyl is intended to include both branched and straight -chain saturated aliphatic hydrocarbon groups having the specified number of carbon atoms (for example, "Ci-Cio” denotes alkyl having 1 to 10 carbon atoms); “alkoxy” represents an alkyl group of indicated number of carbon atoms attached through an oxygen bridge; “cycloalkyl” is intended to include saturated ring groups, including mono-, bi-, or poly- cyclic ring systems, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, and adamantyl; and “bicycloalkyl” is intended to include saturated bicyclic ring groups such as [3.3. O]bicyclooctane, [4.3.0]bicyclononane,
  • alkenyl is intended to include hydrocarbon chains of either a straight or branched configuration and one or more unsaturated carbon-carbon bonds which may occur in any stable point along the chain, such as ethenyl, propenyl and the like; and "alkynyl” is intended to include hydrocarbon chains of either a straight or branched configuration and one or more triple carbon-carbon bonds which may occur in any stable point along the chain, such as ethynyl, propynyl and the like.
  • alkylene alkenylene
  • phenylene phenylene
  • alkylene alkenylene
  • phenylene phenylene
  • alkyl alkenylene
  • phenylene phenylene
  • alkyl alkenylene
  • phenylene phenylene
  • Halo or "halogen” as used herein refers to fluoro, chloro, bromo and iodo; and "counterion” is used to represent a small, negatively charged species such as chloride, bromide, hydroxide, acetate, sulfate and the like.
  • aryl or “aromatic residue” is intended to mean phenyl or naphthyl optionally substituted with 0-3 groups independently selected from methyl, methoxy, amino, hydroxy, halogen, C ⁇ -C 6 alkoxy, C ⁇ -C 6 alkyl, CF , S(0) m CH 3 , -N(CH 3 ) 2 - C1-C4 haloalkyl, methylenedioxydiyl, ethylenedioxydiyl; the term “arylalkyl” represents an aryl group attached through an alkyl bridge.
  • heteroaryl refers to aromatic heterocyclic groups. Such heteroaryl groups are preferably 5-6 membered monocylic groups or 8-10 membered fused bicyclic groups. Examples of such heteroaryl groups include, but are not limited to pyridyl (pyridinyl), pyrimidinyl, furanyl (furyl), t iazolyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, indolyl, isoxazolyl, oxazolyl, pyrazinyl, pyridazinyl, benzofuranyl, benzothienyl, benzimidazolyl, quinolinyl, or isoquinolinyl.
  • chiral amine refers to any amine containing compound that also contains a chiral center.
  • Such compounds include, by way of example and without limitation, either enantiomer of cinchonidine, ephedrine, 2-phenylglycinol, 2-amino-3- methoxy-1-propanol, quinidine and pseudoephedrine.
  • pharmaceutically acceptable salts refer to derivatives of the disclosed compounds wherein the parent compound of Formula I is modified by making acid or base salts of the compound of Formula I.
  • pharmaceutically acceptable salts include, but are not limited to, mineral or organic acid salts of basic residues such as amines; alkali or organic salts of acidic residues such as carboxylic acids; and the like.
  • Prodrugs are considered to be any covalently bonded carriers which release the active parent drug according to Formula I in vi vo when such prodrug is administered to a mammalian subject.
  • Prodrugs of the compounds of Formula I are prepared by modifying functional groups present in the compounds in such a way that the modifications are cleaved, either in routine manipulation or in vivo, to the parent compounds.
  • Prodrugs include compounds of Formula I wherein hydroxyl, amino, sulfhydryl, or carboxyl groups are bonded to any group that, when administered to a mammalian subject, cleaves to form a free hydroxyl, amino, sulfhydryl, or carboxyl group respectively.
  • Examples of prodrugs include, but are not limited to, acetate, formate and benzoate derivatives of alcohol and amine functional groups in the compounds of Formula I, and the like.
  • prodrug forms of the compounds of the present invention include the following esters: methyl; ethyl; isopropyl; methylcarbonyloxymethyl-; ethylcarbonyloxymethyl-; t-butylcarbonyloxymethyl-; cyclohexylcarbonyloxymethyl- , ⁇
  • the pharmaceutically acceptable salts of the compounds of Formula I include the conventional non- toxic salts or the quaternary ammonium salts of the compounds of Formula I formed, for example, from non- toxic inorganic or organic acids.
  • such conventional non-toxic salts include those derived from inorganic acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like; and the salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxybenzo ⁇ c, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, lsethionic, and the like.
  • the pharmaceutically acceptable salts of the present invention can be synthesized from the compounds of Formula I which contain a basic or acidic moiety by conventional chemical methods. Generally, the salts are prepared by reacting the free base or acid with stoichiometnc amounts or with an excess of the desired salt- forming inorganic or organic acid or base in a suitable solvent or various combinations of solvents.
  • the pharmaceutically acceptable salts of the acids of Formula I can be formed with an appropriate amount of a base, such as an alkali or alkaline earth metal hydroxide e.g. sodium, potassium, lithium, calcium, or magnesium, or an organic base such as an amine, e.g., dibenzylethylenediamme, tri ethylamine, piperid e, pyrrolidine, benzylamme and the like, or a quaternary ammonium hydroxide such as tetramethylammoinum hydroxide and the like.
  • a base such as an alkali or alkaline earth metal hydroxide e.g. sodium, potassium, lithium, calcium, or magnesium
  • an organic base such as an amine, e.g., dibenzylethylenediamme, tri ethylamine, piperid e, pyrrolidine, benzylamme and the like, or a quaternary ammonium hydroxide such as tetramethylammoinum hydrox
  • pharmaceutically acceptable salts of the compounds of the invention can be prepared by reacting the free acid or base forms of these compounds with a stoichiometric amount of the appropriate base or acid, respectively, in water or in an organic solvent, or in a mixture of the two; generally, nonaqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile are preferred. Lists of suitable salts are found in Remington ' Pharmaceutical Sciences. 17th ed., Mack Publishing Company, Easton, PA, 1985, p. 1418, the disclosure of which is hereby incorporated by reference.
  • the compounds of the present invention can be prepared in a number of ways well known to one skilled in the art of organic synthesis.
  • the compounds of the present invention can be synthesized using the methods described below, together with synthetic methods known in the art of synthetic organic chemistry, or variations thereon as appreciated by those skilled in the art. Preferred methods include, but are not limited to, those described below. All references cited herein are hereby incorporated their entirety herein by reference.
  • a convenient method for the synthesis of the compounds of this invention utilizes a dipolar cycloaddition of nitrones with appropriate dipolarophiles to the isoxazolidine rings present in compounds of Formula I (for a review of 1,3 -dipolar cycloadditions of nitrones, see Org. React. 36, 1, Confalone, et al) .
  • Scheme I describes one synthetic sequence to the compounds of this invention.
  • An appropriately substituted nitrile aldehyde (1-1) is treated with hydroxyamine in the presence of K2C03 in CH2C12 to give a nitrone(I-2) , which undergoes a 1,3-dipolar cycloaddtion to a suitably substituted alkene to afford the isoxazolidine (1-3) .
  • Hydrolysis of the ester using conventional methods known to one skilled in the art of organic synthesis gives the desired acids.
  • Intermediates containing alkali-sensitive functionality, such as nitrile may be deesterified with excellent chemoselectivity using trimethylsilanolate according to the procedure of Laganis and Ehenard (Tetrahedron Lett.
  • racemic p-amino acids may be purchased commercially or, as is shown in Scheme II, Method 1, prepared from the appropriate aldehyde, malonic acid and ammonium acetate according to the procedure of Johnson and Livak (J. Am. Chem. Soc. 1936, 58, 299) .
  • Racemic ⁇ -substituted- ⁇ -amino esters may be prepared through the reaction of dialkylcuprates or alkyllithiums with 4-benzoyloxy-2-azetidinone followed by treatment with anhydrous ethanol (Scheme I, Method 2) or by reductive a ination of ⁇ -keto esters as is described in WO9316038. (Also see Rico et al., J.
  • Enantiomencally pure ⁇ - substituted- ⁇ -ammo acids can be obtained through the optical resolution of the racemic mixture or can be prepared using numerous methods, including: Arndt- Eistert homologation of the corresponding ⁇ -amino acids as shown in Scheme II, Method 3 (see Meier, and Zeller, An ⁇ ew. Chem. Int. Ed. En ⁇ l. 1975, IA, 32; Rodriguez, et al. Tetrahedron Lett. 1990, H, 5153; Greenlee, J. Med. Chem.
  • N 2 -subst ⁇ tuted diaminopropionic acid derivatives can be carried out via Hof fman rearrangement of a wide variety of asparagine derivatives as described in Synthesis, 266-267, (1981).
  • the dipolarophiles used to prepare the compounds of this invention may be prepared by numerous methods.
  • the ⁇ -alkenoic ester class of dipolarophile may be purchased commercially or prepared by oxidation of the corresponding ⁇ -alkenols by the method of Corey and Schmidt (Tetrahedron Let . 1979, 399, Scheme VI).
  • N 2 -Cbz-L-2,3-diaminopropionic acid (10 mmol, 2.39 g) was dissolved in 20 mL methanol and 20 mL 4 N HCl in dioxane and the solution was stirred for 4 hours and then concentrated to give a solid. The solid was washed with ether several times to give 2.50 g (87%) product.
  • Part F MPfhvl N ⁇ -Boc- (S) -2. -diaminopropionate Formic arid salt.
  • Dry HCl gas was bubled through a solution of Cis-3- [2- [2-methyl-3- (4-cyanophenyl) -isoxazolidin-5- yl] acetyl] amino-N- (3-methylphenylsulfonyl) -L-alanine methyl ester (580mg 1.16mmol) in dry CHCI3 containing anhydrous methanol (55mg, 1.7mmol), cooled with salt ice- water bath, at 0 °C for 5hrs. The resulting solution was then kept at 0 °C for 6hrs and at 15 °C for 12hrs.
  • Cis-3- [2- [2 -methyl -3- [4- (aminoiminomethyl ) phenyl ] - isoxazolidin-5-yl] -acetyl] amino-N- (3- methylphenylsulfonyl) -L-alanine methyl ester (lOO g, 0.19mmol) was dissolved in 3N HCl (3ml) .
  • the resulting solution was stirred at rt for 36hrs and then concentrated to yield the acid as an amorphous solid (90mg, 90% yield) .
  • the acid was further purified by reverse HPLC using water and 0.1% TFA in acetonitrile as eluent.
  • Dry HCl gas was bubled through a solution of Cis-3- [2- [2-benzyl-3- (4-cyanophenyl) -isoxazolidin-5- yl ] acetyl] amino-N- (3 -methylphenylsulfonyl ) -L-alanine methyl ester (510mg 1.13mmol) in dry CHCI3 containing anhydrous methanol (53mg, 1.7mmol), cooled with salt ice- water bath, at 0 °C for 5hrs. The resulting solution was then kept at 0 °C for 6hrs and at 15 °C for 12hrs.
  • This compound was prepared analogously to Example 1.
  • Cis-3- [2- [2-methyl-3- (4-cyanophenyl ) -isoxazolidin-5- yl] acetyl] amino-N- (3-methylphenylsulfonyl) -L-alanine methyl ester (600mg, 1.2mmol) was dissolved in aqueous acetic acid(16ml, v:v, 1:1) and zinc(1.17g, 18mmol) was added. The resulting suspension was stirred vigorously at rt for 2hrs, then was filtered. The filtration was evaporated to dryness. The residue was dissolved in aqueous NaHCO3(10ml) and the cloudy solution was evaporated to dyness again. The remaining solid was extracted with ethyl acetate. Removal of ethyl acetate gave the 1, 3-aminoalcohol as an oil, which was directly used in the next reaction.
  • the compounds of this invention possess antiplatelet efficacy, as evidenced by their activity in standard platelet aggregation assays or platelet fibrinogen binding assays, as described below.
  • a compound is considered to be active in these assays if it has an IC50 value of less than about 1 mM.
  • Platelet aggregation and fibrinogen binding assays which may be used to demonstrate the antiplatelet activity of the compounds of the invention are described below.
  • Platelet Aggregation Assay Venous blood was obtained from the arm of a healthy human donor who was drug- free and aspirin- free for at least two weeks prior to blood collection. Blood was collected into 10 mL citrated Vacutainer tubes. The blood was centrifuged for 15 minutes at 150 x g at room temperature, and platelet-rich plasma (PRP) was removed. The remaining blood was centrifuged for 15 minutes at 1500 x g at room temperature, and platelet-poor plasma (PPP) was removed. Samples were assayed on a aggregometer (PAP-4 Platelet Aggregation Profiler) , using PPP as the blank (100% transmittance) .
  • PAP-4 Platelet Aggregation Profiler PPP as the blank (100% transmittance
  • Ester prodrugs were preincubated (10 ⁇ 3 M F.C.) with 100 IU/mL Porcine liver esterase (Sigma Chemical Co. , St. Louis, MO, #E-3128) for 2 hours at 37 °C. Aliquots are then diluted in 0.1 M Tris, pH 7.4, to the desired concentrations. Aliquots of 20 ⁇ l of the esterase pretreated prodrugs are added to 200 ⁇ l of human
  • GPIIb/IIIa-fibrinogen binding ELISA purified GPIIb/IIIa (148.8 ⁇ g/mL) ; biotinylated fibrinogen ( ⁇ 1 mg/mL or 3000 nM) ; anti-biotm alkaline phosphatase conjugate (Sigma no. A7418) ; flat-bottom, high binding, 96-well plates (Costar
  • phosphatase substrate (Sigma 104) (40 mg capsules); bovine serum albumin (BSA) (Sigma no. A3294); Alkaline Phosphatase buffer - 0.1 M glycme-HCl, 1 mM MgCl 2 *6H 2 0, 1 mM ZnCl 2 , pH 10.4; Binding buffer - 20 mM T ⁇ s-HCl, 150 mM NaCl, 1 mM
  • Pipet 11 ⁇ L of test compound (10X the concentration to be tested in Dilution buffer) into duplicate wells. Pipet 11 ⁇ L Dilution buffer into non-specific and total binding wells. Add 100 ⁇ L Biotmylated fibrinogen (1/133 in Dilution buffer, final concentration 20 nM) to each well. Incubate plates for 3 hours at room temperature on a plate shaker. Discard assay solution and wash twice with 300 ⁇ L Binding buffer per well. Add 100 mL Anti-biotm alkaline phosphatase conjugate (1/1500 in Dilution buffer) to each well. Incubate plates for 1 hour at room temperature on plate shaker. Discard conjugate and wash twice with 300 51 Binding buffer per well.
  • Plat l t-Fibrinogen Binding Assay Binding of 125 I- fibrinogen to platelets was performed as described by Bennett et al. (1983) Proc. Natl. Acad. Sci. USA 80: 2417-2422, with some modifications as described below. Human PRP (h-PRP) was applied to a Sepharose column for the purification of platelet fractions. Aliquots of platelets (5 X 10 8 cells) along with 1 M calcium
  • U chloride were added to removable 96 well plates prior to the activation of the human gel purified platelets (h-GPP) .
  • Activation of the human gel purified platelets was achieved using ADP, collagen, arachidonate, epmephr e, and/or thrombin in the presence of the ligand, 125 I-f ⁇ bnnogen.
  • the 125 I-f ⁇ bnnogen oound to the activated platelets was separated from the free form by centrifugation and then counted on a gamma counter.
  • the test compounds were added at various concentrations prior to the activation of the platelets.
  • the compounds of Formula I of the present invention may also possess thrombolytic efficacy, that is, they are capable of lysing (breaking up) already formed platelet-rich fibrin blood clots, and thus are useful in treating a thrombus formation, as evidenced by their activity m the tests described below.
  • Preferred compounds of the present invention for use in thrombolysis include those compounds having an IC5 0 value (that is, the molar concentration of the compound capable of achieving 50% clot lysis) of less than about 1 ⁇ M, more preferably an IC 50 value of less than about 0.1 ⁇ M.
  • Thrombolytic Assav Venous blood was obtained from the arm of a healthy human donor who was drug-free and aspirin free for at least two weeks prior to blood collection, and placed into 10 ml citrated Vacutainer tubes. The blood was centrifuged for 15 minutes at 1500 x g at room temperature, and platelet rich plasma (PRP) was removed. To the PRP was then added 1 x 10 "3 M of the agonist ADP, epmephrme, collagen, arachidonate, serotonin or thrombin, or a mixture thereof, and the PRP incubated for 30 minutes. The PRP was centrifuged for 12 minutes at 2500 x g at room temperature. The supernatant was then poured off, and the platelets remaining in the test tube were resuspended in platelet poor plasma (PPP), which served as a plasminogen source.
  • PPP platelet poor plasma
  • the compounds of Formula I of the present invention are also useful for administration m combination with anti-coagulant agents such as warfarin or heparin, or antiplatelet agents such as aspirin, piroxicam or ticlopidine, or thrombin inhibitors such as boropeptides, hirudin or argatroban, or thrombolytic agents such as tissue plasminogen activator, anistreplase, urokinase or streptokinase, or combinations thereof.
  • anti-coagulant agents such as warfarin or heparin
  • antiplatelet agents such as aspirin, piroxicam or ticlopidine
  • thrombin inhibitors such as boropeptides, hirudin or argatroban
  • thrombolytic agents such as tissue plasminogen activator, anistreplase, urokinase or streptokinase, or combinations thereof.
  • the compounds of Formula I of the present invention may also be useful as antagonists of other tegrins such as for example, the a v / 3 or vitronectin receptor, a 4 b ⁇ or as i and as such may also have utility n the treatment and diagnosis of osteoporosis, cancer metastasis, diabetic ret opathy, rheumatoid arthritis, inflammation, and autoimmune disorders.
  • the compounds of Formula I of the present invention may be useful for the treatment or prevention of other diseases which involve cell adhesion processes, including, but not limited to, infanimation, bone degradation, rheumatoid arthritis, asthma, allergies, adult respiratory distress
  • ⁇ 8 syndrome graft versus host disease, organ transplantation, septic shock, psoriasis, eczema, contact dermatitis, osteoporosis, osteoarthritis, atherosclerosis, metastasis, wound healing, diabetic retinopathy, inflammatory bowel disease and other autoimmune diseases.
  • Table A sets forth the antiplatelet activity of representative compounds of the present invention.
  • the indicated compounds were tested for their ability to inhibit platelet aggregation (using platelet rich plasma (PRP) ) .
  • the IC 50 value (the concentration of antagonist which inhibits platelet aggregation by 50% relative to a control lacking the antagonist) is shown.
  • the compounds of the present invention can be administered in such oral dosage forms as tablets, capsules (each of which includes sustained release or timed release formulations), pills, powders, granules, elixirs, tinctures, suspensions, syrups, and emulsions. Likewise, they may also be administered in intravenous (bolus or infusion), intraperitoneal, subcutaneous, or intramuscular form, all using dosage forms well known to those of ordinary skill in the pharmaceutical arts. An effective but non-toxic amount of the compound desired can be employed as an anti-aggregation agent. Finally, the compounds of the invention may also be administered mtranasally.
  • the compounds of this invention can be administered by any means that produces contact of the active agent with the agent's site of action, glycoprote Ilb/IIIa (GPIIb/IIIa), in the body of a mammal. They can be administered by any conventional means available for use n conjunction with pharmaceuticals, either as individual therapeutic agents or in a combination of therapeutic agents, such as a second antiplatelet agent such as aspirin or ticlopidine which are agonist- specific. They can be administered alone, but generally administered with a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice.
  • a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice.
  • the dosage regimen for the compounds of the present invention will, of course, vary depending upon known factors, such as the pharmacodynamic characteristics of the particular agent and its mode and route of administration; the species, age, sex, health, medical condition, and weight of the recipient; the nature and extent of the symptoms; the kind of concurrent treatment; the frequency of treatment; the route of administration, the renal and hepatic function of the patient, and the effect desired.
  • An ordinarily skilled physician or veterinarian can readily determine and prescribe the effective amount of the drug required to prevent, counter, or arrest the progress of the condition.
  • the daily oral dosage of each active ingredient when used for the indicated effects, will range between about 0.001 to 1000 mg/kg of body weight, preferably between about 0.01 to 100 mg/kg of body weight per day, and most preferably between about 1.0 to 20 mg/kg/day.
  • the most preferred doses will range from about 1 to about 10 mg/kg/minute during a constant rate infusion.
  • compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three, or four times daily.
  • the compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal routes, using those forms of transdermal skin patches wall known to those of ordinary skill in that art.
  • the dosage administration will, of course, be continuous rather than intermittant throughout the dosage regimen.
  • the compounds herein described in detail can form the active ingredient, and are typically administered in admixture with suitable pharmaceutical diluents, excipients, or carriers (collectively referred to herein as carrier materials) suitably selected with respect to the intended form of administration, that is, oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional pharmaceutical practices.
  • the active drug component can be combined with an oral, non-toxic, pharmaceutically acceptable, inert carrier such as lactose, starch, sucrose, glucose, methyl callulose, magnesium stearate, dicalcium phosphate, calcium sulfate, mannitol, sorbitol and the like; for oral administration in liquid form, the oral drug components can be combined w th any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like.
  • suitable binders, lubricants, disintegrating agents, and coloring agents can also be incorporated into the mixture.
  • Suitable binders include starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth, or sodium algmate, carboxymethylcellulose, polyethylene glycol, waxes, and the like.
  • Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like.
  • Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.
  • the compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamallar vesicles, and multilamellar vesicles.
  • liposomes can be
  • ?2 formed from a variety of phospholipids, such as cholesterol, stearylamine, or phosphatidylcholines.
  • Compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers.
  • soluble polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxyethylaspartamidephenol, or polyethyleneoxide- polylysine substituted with palmitoyl residues.
  • the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacylates, and crosslinked or amphipathic block copolymers of hydrogels .
  • a drug for example, polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacylates, and crosslinked or amphipathic block copolymers of hydrogels .
  • Dosage forms suitable for administration may contain from about 1 milligram to about 100 milligrams of active ingredient per dosage unit.
  • the active ingredient will ordinarily be present in an amount of about 0.5-95% by weight based on the total weight of the composition.
  • the active ingredient can be administered orally in solid dosage forms, such as capsules, tablets, and powders, or in liquid dosage forms, such as elixirs, syrups, and suspensions. It can also be administered parenterally, in sterile liquid dosage forms.
  • Gelatin capsules may contain the active ingredient and powdered carriers, such as lactose, starch, cellulose derivatives, magnesium stearate, stearic acid, and the like. Similar diluents can be used to make compressed tablets . Both tablets and capsules can be manufactured as sustained release products to provide for continuous release of medication over a period of
  • Compressed tablets can be sugar coated or film coated to mask any unpleasant taste and protect the tablet from the atmosphere, or enteric coated for selective disintegration m the gastrointestinal tract.
  • Liquid dosage forms for oral administration can contain coloring and flavoring to increase patient acceptance.
  • water a suitable oil, saline, aqueous dextrose (glucose) , and related sugar solutions and glycols such as propylene glycol or polyethylene glycols are suitable carriers for parenteral solutions.
  • Solutions for parenteral administration preferably contain a water soluble salt of the active ingredient, suitable stabilizing agents, and if necessary, buffer substances.
  • Antioxidizmg agents such as sodium bisulfite, sodium sulfite, or ascorbic acid, either alone or combined, are suitable stabilizing agents.
  • citric acid and its salts and sodium EDTA are also used.
  • parenteral solutions can contain preservatives, such as benzalkonium chloride, methyl- or propyl -paraben, and chlorobutanol.
  • Suitable pharmaceutical carriers are described in Remington's Pharmaceutical . ⁇ IP ⁇ PS. Mack Publishing
  • a large number of unit capsules are prepared by filling standard two-piece hard gelatin capsules each with 1-20 milligrams of powdered active ingredient, 150 milligrams of lactose, 50 milligrams of cellulose, and 6 milligrams magnesium stearate.
  • a mixture of active ingredient m a digestable oil such as soybean oil, cottonseed oil or olive oil is prepared and injected by means of a positive
  • a large number of tablets are prepared by conventional procedures so that the dosage unit was 1-20 milligrams of active ingredient, 0.2 milligrams of colloidal silicon dioxide, 5 milligrams of magnesium stearate, 275 milligrams of microcrystalline cellulose, 11 milligrams of starch and 98.8 milligrams of lactose.
  • Appropriate coatings may be applied to increase palatability or delay absorption.
  • a parenteral composition suitable for administration by injection is prepared by stirring 1.5% by weight of active ingredient in 10% by volume propylene glycol and water. The solution is made isotonic with sodium chloride and sterilized.
  • An aqueous suspension is prepared for oral administration so that each 5 mL contain 1-20 mg of finely divided active ingredient, 200 mg of sodium carboxymethyl cellulose, 5 mg of sodium benzoate, 1.0 g of sorbitol solution, U.S. P., and 0.025 mL of vanillin.
  • the compounds of the present invention may be administered m combination with a second therapeutic agent selected from: an an i-coagulant agent such as warfarin or heparin; an anti-platelet agent such as aspirin, piroxicam or ticlopidine; a thrombin inhibitor such as a boropeptide thrombin inhibitor, or hirudin; or a thrombolytic agent such as plasminogen activators, such as tissue plasminogen activator, anistreplase, urokinase or streptokinase.
  • a second therapeutic agent selected from: an an i-coagulant agent such as warfarin or heparin; an anti-platelet agent such as aspirin, piroxicam or ticlopidine; a thrombin inhibitor such as a boropeptide thrombin inhibitor, or hirudin; or a thrombolytic agent such as plasminogen activators, such as tissue plasminogen activator, anistreplase, uro
  • the compound of Formula I may be formulated together with the second therapeutic agent in a single dosage unit (that is, combined together in one capsule, tablet, powder, or liquid, etc.).
  • the compound of Formula I and the second therapeutic agent may be administered essentially at the same time, or in any order ; for example the compound of Formula I may be administered first, followed by administration of the second agent (anti-coagulant agent, anti-platelet agent, thrombin inhibitor, and/or thrombolytic agent).
  • the administration of the compound of Formula I and the second therapeutic agent occurs less than about one hour apart .
  • a preferable route of administration of the compound of Formula I is oral.
  • the compound of Formula I and the second therapeutic agent are both administered by the same route (that is, for example, both orally) , if desired, they may each be administered by different routes and in different dosage forms (that is, for example, one component of the combination product may be administered orally, and another component may be administered intravenously) .
  • the dosage of the compound of Formula I when administered alone or in combination with a second therapeutic agent may vary depending upon various factors such as the phar acodynamic characteristics of the particular agent and its mode and route of administration, the age, health and weight of the
  • a daily dosage may be about 0.1 to 100 milligrams of the compound of Formula I and about 1 to 7.5 milligrams of the anticoagulant, per kilogram of patient body weight.
  • the novel compounds of this invention generally may be present in an amount of about 1 to 10 milligrams per dosage unit, and the anticoagulant in an amount of about 1 to 5 milligrams per dosage unit.
  • a daily dosage may be about 0.01 to 25 milligrams of the compound of Formula I and about 50 to 150 milligrams of the additional antiplatelet agent, preferably about 0.1 to 1 milligrams of the compound of Formula I and about 1 to 3 milligrams of antiplatelet agents, per kilogram of patient body weight .
  • a daily dosage may be about 0.1 to 1 milligrams of the compound of Formula I, per kilogram of patient body weight and, in the case of the thrombolytic agents, the usual dosage of the thrombolyic agent when administered alone may be reduced by about 70-80% when administered with a compound of Formula I .
  • the amount of each component in a typical daily dosage and typical dosage form may be reduced relative to the usual dosage of the agent when administered alone, in view of the additive or synergistic effect of the therapeutic agents when administered in combination.
  • one active ingredient may be enteric coated.
  • enteric coating one of the active ingredients it is possible not only to minimize the contact between the combined active ingredients, but also, it is possible to control the release of one of these components in the gastrointestinal tract such that one of these components is not released in the stomach but rather is released in the intestines.
  • One of the active ingredients may also be coated with a sustained- release material which effects a sustained-release throughout the gastrointestinal tract and also serves to minimize physical contact between the combined active ingredients.
  • the sustained-released component can be additionally enteric coated such that the release of this component occurs only m the intestine.
  • Still another approach would involve the formulation of a combination product in which the one component is coated with a sustained and/or enteric release polymer, and the other component is also coated with a polymer such as a lowviscosity grade of hydroxypropyl methylcellulose (HPMC) or other
  • kits useful for example, in the inhibition of platelet aggregation, the treatment of blood clots, and/or the treatment of thromboembolic disorders, which comprise one or more containers containing a pharmaceutical composition comprising a therapeutically effective amount of a compound of Formula I.
  • kits may further include, if desired, one or more of various conventional pharmaceutical kit components, such as, for example, containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art.
  • Instructions, either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and/or guidelines for mixing the components may also be mcluded in the kit.

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Abstract

L'invention porte d'une manière générale sur des carbamates et sur des hydroxylamines cycliques servant d'antagonistes du complexe récepteur de fibrinogène de la glycoprotéine plaquettaire IIB/IIIA, sur des préparations pharmaceutiques contenant lesdits composés, sur leur procédé de préparation et sur des procédés d'utilisation de ces composés pour empêcher l'agrégation plaquettaire, sur leur utilisation comme thrombolytiques et/ou pour le traitement de troubles d'ordre thromboemboliques.
PCT/US1997/013761 1996-08-15 1997-08-13 Carbamates et isoxazolidines cycliques antagonistes de l'iib/iiia WO1998006707A1 (fr)

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AU39706/97A AU3970697A (en) 1996-08-15 1997-08-13 Cyclic carbamates and isoxazolidines as iib/iiia antagonists
CA002263311A CA2263311A1 (fr) 1996-08-15 1997-08-13 Carbamates et isoxazolidines cycliques antagonistes de l'iib/iiia
EP97937112A EP0922039A1 (fr) 1996-08-15 1997-08-13 Carbamates et isoxazolidines cycliques antagonistes de l'iib/iiia
JP10509820A JP2000516230A (ja) 1996-08-15 1997-08-13 Ｉｉｂ／ｉｉｉａ拮抗薬としての環状カルバメート類およびイソオキサゾリジン類

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WO2000000481A1 (fr) * 1998-06-29 2000-01-06 Du Pont Pharmaceuticals Company Isoxazolidines et carbamates cycliques utilises comme antagonistes de iib/iiia
WO2001074774A1 (fr) 2000-04-05 2001-10-11 Daiichi Pharmaceutical Co., Ltd. Derives ethylenediamine
CN102803214A (zh) * 2009-06-24 2012-11-28 贝林格尔.英格海姆国际有限公司 新化合物、与之相关的药物组合物和方法

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WO1995014682A1 (fr) * 1993-11-24 1995-06-01 The Du Pont Merck Pharmaceutical Company Composes d'isoxazoline utiles en tant qu'antagonistes du recepteur fibrinogene
WO1996037482A1 (fr) * 1995-05-24 1996-11-28 The Du Pont Merck Pharmaceutical Company Isoxazolines ameliorees utilisees comme antagonistes du recepteur de fibrinogene

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WO2000000481A1 (fr) * 1998-06-29 2000-01-06 Du Pont Pharmaceuticals Company Isoxazolidines et carbamates cycliques utilises comme antagonistes de iib/iiia
US6265400B1 (en) 1998-06-29 2001-07-24 Dupont Pharmaceuticals Company Cyclic carbamates and isoxazolidines as IIb/IIIa antagonists
WO2001074774A1 (fr) 2000-04-05 2001-10-11 Daiichi Pharmaceutical Co., Ltd. Derives ethylenediamine
US7192968B2 (en) 2000-04-05 2007-03-20 Daiichi Pharmaceutical Co., Ltd. Ethylenediamine derivatives
JP4495896B2 (ja) * 2000-04-05 2010-07-07 第一三共株式会社 エチレンジアミン誘導体
US7935824B2 (en) 2000-04-05 2011-05-03 Daiichi Pharmaceutical Co., Ltd. Ethylenediamine derivatives
CN102803214A (zh) * 2009-06-24 2012-11-28 贝林格尔.英格海姆国际有限公司 新化合物、与之相关的药物组合物和方法

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CA2263311A1 (fr) 1998-02-19
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AU3970697A (en) 1998-03-06

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