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WO1998002536A3 - Preparation of pichia methanolica auxotrophic mutants - Google Patents

Preparation of pichia methanolica auxotrophic mutants Download PDF

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Publication number
WO1998002536A3
WO1998002536A3 PCT/US1997/012582 US9712582W WO9802536A3 WO 1998002536 A3 WO1998002536 A3 WO 1998002536A3 US 9712582 W US9712582 W US 9712582W WO 9802536 A3 WO9802536 A3 WO 9802536A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
culturing
culture medium
methanolica
preparation
Prior art date
Application number
PCT/US1997/012582
Other languages
French (fr)
Other versions
WO1998002536A2 (en
Inventor
Christopher K Raymond
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/703,808 external-priority patent/US5736383A/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to IL12807297A priority Critical patent/IL128072A0/en
Priority to EP97936103A priority patent/EP0946734B1/en
Priority to CA002261020A priority patent/CA2261020C/en
Priority to AU38856/97A priority patent/AU708572B2/en
Priority to DE69735369T priority patent/DE69735369T2/en
Priority to JP50632398A priority patent/JP2002514049A/en
Publication of WO1998002536A2 publication Critical patent/WO1998002536A2/en
Publication of WO1998002536A3 publication Critical patent/WO1998002536A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods for preparing Pichia methanolica cells having auxotrophic mutations are disclosed. The methods comprise the steps of: (a) exposing P. methanolica cells to mutagenizing conditions; (b) culturing the cells from step (a) in a rich medium to allow mutations to become established and replicated in at least a portion of the cells; (c) culturing the cells from step (b) in a culture medium deficient in assimilable nitrogen to deplete cellular nitrogen stores; (d) culturing the cells from step (c) in a defined culture medium comprising an inorganic nitrogen source and an amount of nystatin sufficient to kill growing P. methanolica cells to select for cells having a deficiency in a nutritional gene; and (e) culturing the selected cells from step (d) in a rich culture medium.
PCT/US1997/012582 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants WO1998002536A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
IL12807297A IL128072A0 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
EP97936103A EP0946734B1 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
CA002261020A CA2261020C (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants
AU38856/97A AU708572B2 (en) 1996-07-17 1997-07-14 Preparation of (pichia methanolica) auxotrophic mutants
DE69735369T DE69735369T2 (en) 1996-07-17 1997-07-14 PREPARATION OF AUXOTROPHIC MUTANES FROM PICHIA METHANOLICA
JP50632398A JP2002514049A (en) 1996-07-17 1997-07-14 Production of auxotrophic mutants of Pichia methanolica

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US68350096A 1996-07-17 1996-07-17
US08/683,500 1996-08-26
US08/703,808 1996-08-26
US08/703,808 US5736383A (en) 1996-08-26 1996-08-26 Preparation of Pichia methanolica auxotrophic mutants

Publications (2)

Publication Number Publication Date
WO1998002536A2 WO1998002536A2 (en) 1998-01-22
WO1998002536A3 true WO1998002536A3 (en) 1998-02-26

Family

ID=27103122

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/012582 WO1998002536A2 (en) 1996-07-17 1997-07-14 Preparation of pichia methanolica auxotrophic mutants

Country Status (6)

Country Link
JP (1) JP2002514049A (en)
CN (1) CN1238806A (en)
AU (1) AU708572B2 (en)
CA (1) CA2261020C (en)
IL (1) IL128072A0 (en)
WO (1) WO1998002536A2 (en)

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NZ510744A (en) 1998-09-23 2003-02-28 Zymogenetics Inc Cytokine receptor zalpha11
DE60005135T3 (en) 1999-01-07 2015-04-09 Zymogenetics, Inc. SOLUBLE RECEPTORS BR43X2 AND METHOD FOR THEIR THERAPEUTIC USE
US7833529B1 (en) 1999-01-07 2010-11-16 Zymogenetics, Inc. Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor
CN101575377B (en) 1999-03-09 2012-12-26 津莫吉尼蒂克斯公司 Human cytokine as ligand of zalpha receptor and use thereof
EP1860188B1 (en) 1999-07-07 2011-05-04 ZymoGenetics, Inc. Human cytokine receptor
ATE459716T1 (en) 1999-12-23 2010-03-15 Zymogenetics Inc CYTOKINE ZCYT018
DK1325115T3 (en) 2000-06-26 2016-11-21 Zymogenetics Inc CYTOKINRECEPTOR ZCYTOR17
WO2002066516A2 (en) 2001-02-20 2002-08-29 Zymogenetics, Inc. Antibodies that bind both bcma and taci
US20030103986A1 (en) 2001-05-24 2003-06-05 Rixon Mark W. TACI-immunoglobulin fusion proteins
EP2298354B1 (en) 2001-10-10 2014-03-19 ratiopharm GmbH Remodelling and glycoconjugation of interferon-beta
JP2005508179A (en) 2001-11-05 2005-03-31 ザイモジェネティクス，インコーポレイティド IL-21 antagonist
JP4511349B2 (en) 2002-01-18 2010-07-28 ザイモジェネティクス，インコーポレイティド Cytokine receptor zcytor17 multimer
ES2351678T3 (en) 2002-01-18 2011-02-09 Zymogenetics, Inc. BINDING CITOCINE FOR THE TREATMENT OF ASTHMA AND HYPER-SENSITIVITY OF RESPIRATORY ROUTES.
DE60335536D1 (en) 2002-04-19 2011-02-10 Bristol Myers Squibb Co METHOD FOR DETECTING OR MODULATING THE INTERACTION OF A CYTOKINE RECEPTOR WITH ITS LIGAND
NZ542586A (en) 2003-04-09 2009-11-27 Biogenerix Ag Method of forming a covalent conjugate between a G-CSF peptide and PEG
CA2834275A1 (en) 2004-08-02 2006-02-09 Basf Plant Science Gmbh Method for isolation of transcription termination sequences
CN100347287C (en) * 2004-09-30 2007-11-07 汪和睦 Recombinated multi shape ttansenula yeast, its structural method and application
EP1856156A2 (en) 2005-02-08 2007-11-21 ZymoGenetics, Inc. Anti-il-20, anti-il-22 and anti-il-22ra antibodies and binding partners and methods of using in inflammation
EP2397493A1 (en) 2005-05-12 2011-12-21 ZymoGenetics, Inc. Compositions and methods for modulating immune responses
US7842292B2 (en) 2005-08-09 2010-11-30 Ares Trading S.A. Methods for treating B-cell malignancies using a TACI-Ig fusion molecule
CA2618763A1 (en) 2005-08-09 2007-02-15 Zymogenetics, Inc. Methods for the treatment and prevention of abnormal cell proliferation using taci-fusion molecules
DE602006016965D1 (en) 2005-09-28 2010-10-28 Zymogenetics Inc IL-17A AND IL-17F ANTAGONISTS AND METHOD OF USE
NZ572373A (en) 2006-05-15 2012-02-24 Ares Trading Sa Methods for treating rheumatoid arthritis using a taci-ig fusion molecule
DK2225267T3 (en) 2007-11-21 2015-04-27 Roskilde Uni Polypeptides comprising a isbindende activity
NZ600205A (en) 2008-01-25 2013-12-20 Univ Aarhus Selective exosite inhibition of papp-a activity against igfbp-4
MX2011006483A (en) 2008-12-16 2011-07-13 Novartis Ag Yeast display systems.
US9771576B2 (en) 2012-02-01 2017-09-26 Synthetic Genomics, Inc. Materials and methods for the synthesis of error-minimized nucleic acid molecules
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides
CN111157680B (en) * 2019-12-31 2021-10-26 北京辰安科技股份有限公司 Indoor volatile substance leakage tracing method and device
CN116064597B (en) * 2023-01-17 2024-04-26 北京大学 Directed evolution and darwinian adaptation in mammalian cells by autonomous replication of RNA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017451A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997017451A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Production of gad65 in methylotrophic yeast
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica

Non-Patent Citations (7)

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Title
BIOLOGICAL ABSTRACTS, vol. BA64, Philadelphia, PA, US; abstract no. 50226, TOLSTORUKOV I I ET AL: "HYBRIDIZATION AND GENETIC ANALYSIS OF THE METHANOL UTILIZING YEAST PICHIA-PINUS." XP002051202 *
BIOLOGICAL ABSTRACTS, vol. BA85, Philadelphia, PA, US; abstract no. 37426, TOLSTORUKOV I I ET AL: "GENETIC CONTROL OF PURINE BIOSYNTHESIS IN THE YEAST PICHIA PINUS I. GENUS CONTROLLING THE VI AND VII STEPS." XP002051203 *
GENETIKA 13 (2). 1977 322-329. CODEN: GNKAA5 ISSN: 0016-6758 *
GENETIKA 23 (8). 1987. 1399-1406. CODEN: GNKAA5 ISSN: 0016-6758 *
LAWRENCE C.W.: "Classical mutagenesis techniques", METHODS IN ENZYMOLOGY, vol. 194, 1991, pages 273 - 281, XP002050288 *
SNOW R.: "An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'", NATURE, vol. 211, 9 July 1966 (1966-07-09), pages 206 - 207, XP002050287 *
TOLSTORUKOV I I ET AL: "MUTANTS OF THE METHYLOTROPHIC YEAST PICHIA PINUS DEFECTIVE IN C-2 METABOLISM.", YEAST 5 (3). 1989. 179-186. CODEN: YESTE3 ISSN: 0749-503X, XP002051201 *

Also Published As

Publication number Publication date
CN1238806A (en) 1999-12-15
AU708572B2 (en) 1999-08-05
AU3885697A (en) 1998-02-09
CA2261020A1 (en) 1998-01-22
WO1998002536A2 (en) 1998-01-22
JP2002514049A (en) 2002-05-14
CA2261020C (en) 2004-06-08
IL128072A0 (en) 1999-11-30

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