WO1998000697A1 - Analyseur de dosages immunologiques automatise - Google Patents
Analyseur de dosages immunologiques automatise Download PDFInfo
- Publication number
- WO1998000697A1 WO1998000697A1 PCT/US1997/011245 US9711245W WO9800697A1 WO 1998000697 A1 WO1998000697 A1 WO 1998000697A1 US 9711245 W US9711245 W US 9711245W WO 9800697 A1 WO9800697 A1 WO 9800697A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tube
- sample
- bead
- chamber
- reagent
- Prior art date
Links
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00457—Dispensing or evacuation of the solid phase support
- B01J2219/00459—Beads
- B01J2219/00468—Beads by manipulation of individual beads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00435—Refrigerated reagent storage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0403—Sample carriers with closing or sealing means
- G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0451—Rotary sample carriers, i.e. carousels composed of interchangeable sectors
Definitions
- a sample is mixed with a reagent and a solid support having a bound antigen or antibody, the sample is incubated such that the corresponding antigen or antibody in the sample and a labeled antigen or antibody provided in the reagent can be bound to the antigen or antibody on the solid support, then the solid support is thoroughly washed and the label (fluorescent, radioactive, chemiluminescent, or the like) is detected by an appropriate mechanism, and finally the analyte of interest (antigen or antibody) is quantified from the detected label.
- the label fluorescent, radioactive, chemiluminescent, or the like
- the removable dilution well cover 613 has a central opening 614 for pipette access. Concentric projections 614' and 614" extending from the lower side 613' of the cover 613 help direct a pipette into the mouth 631 of the dilution well 625 and channel waste fluids during well cleaning, respectively.
- the chamber body 615 includes flange 617 retaining O-ring 616 which forms a seal with the rim 618 of the cover 613.
- the dilution well 625 shown as a test tube-like insert configuration in the FIG., is nested in the rotatable spindle 622 during a dilution and mixing mode and a cleaning high speed spinning mode, but it is a separate removable piece from the system in a preferred embodiment.
- the spindle 622 and nested dilution well 625 are centered in the chamber 611 relative to imaginary longitudinal axis ⁇ .
- the dilution well 625 is a non-wettable material, such as polypropylene, to facilitate water removal from the well 625.
- the dilution well 625 can be formed integrally with the spindle 622.
- exit opening 708 of the dispenser device is positioned directly over a mouth of a reaction tube, then an external force (not shown) is applied to the outer end of section 702e of plunger 702 that is sufficient in magnitude to overcome the internal opposing biasing force of spring 704, so as to displace the plunger 702 leftward (in the view of FIG. 7A) a distance sufficient to align bead chamber 702b of the plunger with exit opening 708, at which point the bead contained and waiting in bead chamber 702b drops by the force of gravity out of the bead chamber, through exit opening 708, and into a reaction tube (or to intermediary means such as tubing used to transport the bead to a reaction tube) .
- FIG. 8A there generally is shown a tube washing system 810 provided with a high speed spinning station 820 having an angled, grooved chuck 822 housed within and surrounded by a waste chamber 823. Further details on the configuration of chuck 822 will be developed hereinafter where suitable.
- the high speed spinning station 820 is shown in its nonengaged position relative to tube 840.
- the waste chamber 823 serves as a receptacle for collecting and draining wash water fluid spun out of a tube 840.
- the tube 840 is rotated on its vertical axes z-z by driving the chuck 822 in rotation while the tube is supported at the bottom of the tube (847, 849) via freely rotatable holder 851 which rotates as dictated by the movement of the chuck.
- the tube spin rate will generally range from about 3,000 to about 10,000 rpm.
- the analytical instrument of this invention produces reportable assay results through the processing of specimens and various other components of the chemistry system. This processing involves the control and timing of various internal operations as well as the acquisition and processing of data generated internally or through interaction with an external computer system such as LIS.
- the analytic instrument is an integrated electromechanical apparatus which processes specimens in order to generate test results. It is comprised of all the mechanical hardware, electronic hardware and software required to perform immunoassays described herein. It is anticipated that many different constituents in the sample can be tested by immunoassay by the inventive instrument depending on the selection of the biomaterial bound to the inert support (e.g., bead) in the assay tube.
- the automated design allows reduced user interface (e.g., tests are performed automatically from computer input) including the ability to order, perform and reassay tests reflexively based on test results without operator intervention.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
L'invention a pour objet un instrument (10) d'analyse de dosages immunologiques automatisé, à haut rendement, susceptible de réaliser des tests en grandes quantités sur une large gamme d'analytes, tout en opérant une sélection parmi un ensemble de divers dosages immunologiques pour chaque échantillon donné. L'instrument (10) d'analyse comporte un sous-système, lequel consiste en un système de distribution de billes (203, 203a-c), permettant le stockage et l'attribution d'une bille unique enrobée de biomatériau compatible avec le type sélectionné de dosage immunologique pour un échantillon donné. L'instrument (10) d'analyse comporte un autre sous-système, consistant en un moyen (209, 209a-c) de stocker des lots de réactifs, et permettant le stockage et le retrait, d'une manière auto-scellante, d'un réactif donné parmi une gamme de différents réactifs stockés. De plus, l'invention porte également sur un système combiné réutilisable de dilution d'échantillon et de puits de dilution d'échantillon (211), et sur un sous-système de poste de lavage de tubes (214) à grande vitesse, permettant de supprimer les besoins en tubes d'échantillonnage ou de dosage possédant des chambres intégrées de collecte de fluides résiduaires.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU35827/97A AU3582797A (en) | 1996-06-28 | 1997-06-27 | Automated immunoassay analyzer |
| DE19781837T DE19781837T1 (de) | 1996-06-28 | 1997-06-27 | Automatischer Immunoassay-Analysator |
Applications Claiming Priority (12)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US67099396A | 1996-06-28 | 1996-06-28 | |
| US08/670,996 | 1996-06-28 | ||
| US08/670,995 US5773296A (en) | 1996-06-28 | 1996-06-28 | Bead dispenser and bead dispenser system for immunoassay analysis |
| US08/670,996 US5721141A (en) | 1996-06-28 | 1996-06-28 | Tube washing system |
| US08/670,994 | 1996-06-28 | ||
| US08/672,654 US5885529A (en) | 1996-06-28 | 1996-06-28 | Automated immunoassay analyzer |
| US08/670,995 | 1996-06-28 | ||
| US08/670,993 | 1996-06-28 | ||
| US08/672,654 | 1996-06-28 | ||
| US08/672,653 | 1996-06-28 | ||
| US08/670,994 US5632399A (en) | 1996-06-28 | 1996-06-28 | Self-sealing reagent container and reagent container system |
| US08/672,653 US5723092A (en) | 1996-06-28 | 1996-06-28 | Sample dilution system and dilution well insert therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1998000697A1 true WO1998000697A1 (fr) | 1998-01-08 |
Family
ID=27560226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1997/011245 WO1998000697A1 (fr) | 1996-06-28 | 1997-06-27 | Analyseur de dosages immunologiques automatise |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU3582797A (fr) |
| DE (1) | DE19781837T1 (fr) |
| WO (1) | WO1998000697A1 (fr) |
Cited By (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0962186A1 (fr) * | 1998-06-02 | 1999-12-08 | Bayer Corporation | Système de diagnostic automatisé pour mettre en oeuvre des dosages immunologiques et des dosages de chimie-cliniques selon un algorithme réflexe |
| EP0994355A1 (fr) * | 1998-09-23 | 2000-04-19 | Randox Laboratories Ltd. | Appareil pur traitement d'un dispositif d'essai |
| US6649128B1 (en) | 1998-09-23 | 2003-11-18 | Randox Laboratories Ltd | Assay device processing instrument |
| AU2003200182B2 (en) * | 1998-09-23 | 2005-02-10 | Randox Laboratories Ltd. | Assay device washing module |
| EP1614473A3 (fr) * | 1998-05-01 | 2007-03-14 | Gen-Probe Incorporated | Carousel multiple pour la livraison d'échantillons aux récipients de réaction dans une analysateur automatique |
| US7250303B2 (en) | 2001-07-20 | 2007-07-31 | Ortho-Clinical Diagnostics, Inc. | Chemistry system for a clinical analyzer |
| EP1918721A1 (fr) * | 2006-10-10 | 2008-05-07 | Sysmex Corporation | Ensemble contenant de réactif |
| AU2006230728B2 (en) * | 1998-05-01 | 2008-09-25 | Gen-Probe Incorporated | Incubator for use in an automated diagnostic analyzer |
| AU2006230720B2 (en) * | 1998-05-01 | 2008-10-09 | Gen-Probe Incorporated | Instrument for detecting light emitted by the contents of a reaction receptacle |
| US7547516B2 (en) | 2005-03-10 | 2009-06-16 | Gen-Probe Incorporated | Method for reducing the presence of amplification inhibitors in a reaction receptacle |
| EP1355160A4 (fr) * | 2001-01-23 | 2010-08-04 | Hitachi Ltd | Analyseur automatique |
| US8192992B2 (en) | 1998-05-01 | 2012-06-05 | Gen-Probe Incorporated | System and method for incubating the contents of a reaction receptacle |
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| EP1914556A3 (fr) * | 2006-10-10 | 2015-02-18 | Sysmex Corporation | Analyseur, ensemble contenant un réactif et procédé d'aspiration de réactif |
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|---|---|---|---|---|
| DE10018876A1 (de) * | 2000-04-14 | 2001-10-25 | Mettler Toledo Gmbh | Analysenvorrichtung und Analysiereinrichtung |
| DE102004047822B4 (de) * | 2004-09-29 | 2007-04-05 | Scil Animal Care Company Gmbh | Reagenzträger sowie Transportbehältnis mit einem Reagenzträger |
| CN109580592B (zh) | 2017-09-28 | 2021-10-08 | 深圳市新产业生物医学工程股份有限公司 | 测量室及其工作方法、化学发光测定方法及发光检测仪 |
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Also Published As
| Publication number | Publication date |
|---|---|
| AU3582797A (en) | 1998-01-21 |
| DE19781837T1 (de) | 1999-09-09 |
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