WO1998000521A1 - Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives - Google Patents
Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives Download PDFInfo
- Publication number
- WO1998000521A1 WO1998000521A1 PCT/JP1997/002167 JP9702167W WO9800521A1 WO 1998000521 A1 WO1998000521 A1 WO 1998000521A1 JP 9702167 W JP9702167 W JP 9702167W WO 9800521 A1 WO9800521 A1 WO 9800521A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- serum
- cells
- medium
- physiologically active
- free medium
- Prior art date
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
Definitions
- ⁇ ⁇ ⁇ May be a source of contamination such as viruses, mycoplasmas, or prions.
- a second object of the present invention is to provide a method for efficiently culturing animal cells.
- a third object of the present invention is to provide a method for producing a physiologically active substance in a large amount from animal cells capable of expressing and producing the physiologically active substance.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne: des milieux exempts de sérum caractérisés par le fait qu'ils contiennent de l'insuline et de la peptone tout en étant pratiquement exempts d'albumine et de transferrine; une méthode de culture de cellules d'origine animale caractérisée par le fait que l'on fait croître les cellules dans les milieux susmentionnés; et un procédé de production de substances physiologiquement actives caractérisé en ce qu'on procède à la culture de cellules d'origine animale, pouvant produire lesdites substances, dans les milieux susmentionnés et que l'on récupère les substances ainsi produites dans les cultures. Les milieux exempts de sérum présentés permettent une prolifération suffisante des cellules, facilitent la récupération des substances physiologiquement actives dans les cultures, et ils sont rarement contaminés, permettant ainsi la mise en oeuvre de la méthode de culture et du procédé de production susmentionnés.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16875996 | 1996-06-28 | ||
| JP8/168759 | 1996-06-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO1998000521A1 true WO1998000521A1 (fr) | 1998-01-08 |
Family
ID=15873915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP1997/002167 WO1998000521A1 (fr) | 1996-06-28 | 1997-06-23 | Milieux exempts de serum, methode de culture de cellules d'origine animale, et procede de production de substances physiologiquement actives |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO1998000521A1 (fr) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002529072A (ja) * | 1998-11-06 | 2002-09-10 | スターンベルド バイオテクノロジー ノース アメリカ， インコーポレイテッド | 組換えヒトエリスロポイエチンを産生する細胞の大量培養のための方法 |
| JP2010273683A (ja) * | 2003-03-03 | 2010-12-09 | Glaxosmithkline Biologicals Sa | 動物を含まない細胞培養方法 |
| JP2014100148A (ja) * | 2007-12-27 | 2014-06-05 | Baxter Internatl Inc | 細胞培養プロセス |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03180175A (ja) * | 1989-12-07 | 1991-08-06 | Snow Brand Milk Prod Co Ltd | 無血清培地 |
| JPH04234982A (ja) * | 1990-12-27 | 1992-08-24 | Green Cross Corp:The | 無血清培地、動物細胞の培養方法および異種蛋白質の製造方法 |
| JPH05207874A (ja) * | 1992-01-28 | 1993-08-20 | Mitsui Toatsu Chem Inc | 無血清培地 |
-
1997
- 1997-06-23 WO PCT/JP1997/002167 patent/WO1998000521A1/fr active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03180175A (ja) * | 1989-12-07 | 1991-08-06 | Snow Brand Milk Prod Co Ltd | 無血清培地 |
| JPH04234982A (ja) * | 1990-12-27 | 1992-08-24 | Green Cross Corp:The | 無血清培地、動物細胞の培養方法および異種蛋白質の製造方法 |
| JPH05207874A (ja) * | 1992-01-28 | 1993-08-20 | Mitsui Toatsu Chem Inc | 無血清培地 |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002529072A (ja) * | 1998-11-06 | 2002-09-10 | スターンベルド バイオテクノロジー ノース アメリカ， インコーポレイテッド | 組換えヒトエリスロポイエチンを産生する細胞の大量培養のための方法 |
| JP2010273683A (ja) * | 2003-03-03 | 2010-12-09 | Glaxosmithkline Biologicals Sa | 動物を含まない細胞培養方法 |
| JP2014100148A (ja) * | 2007-12-27 | 2014-06-05 | Baxter Internatl Inc | 細胞培養プロセス |
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