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WO1998053085A1 - Maturation des fruits par genie genetique - Google Patents

Maturation des fruits par genie genetique Download PDF

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Publication number
WO1998053085A1
WO1998053085A1 PCT/GB1998/001297 GB9801297W WO9853085A1 WO 1998053085 A1 WO1998053085 A1 WO 1998053085A1 GB 9801297 W GB9801297 W GB 9801297W WO 9853085 A1 WO9853085 A1 WO 9853085A1
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WO
WIPO (PCT)
Prior art keywords
seq
cdna
sequence
type
clone
Prior art date
Application number
PCT/GB1998/001297
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English (en)
Inventor
Colin Roger Bird
Rosybel De Jesus Medina-Suarez
Graham Barron Seymour
Original Assignee
Zeneca Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Limited filed Critical Zeneca Limited
Priority to AU72257/98A priority Critical patent/AU7225798A/en
Publication of WO1998053085A1 publication Critical patent/WO1998053085A1/fr
Priority to US09/949,052 priority patent/US20020026657A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life

Definitions

  • This invention relates generally to the modification of a plant phenotype by the regulation of plant gene expression. More specifically it relates to the modulation of the ripening and/or tissue senescence characteristics and plants derived therefrom.
  • Two principal methods for the control of expression are known, viz.: overexpression and underexpression. Overexpression is achieved by insertion of one or more than one extra copies of the selected gene. It is, however, not unknown for plants or their progeny, originally transformed with one or more than one extra copy of a nucleotide sequence, to exhibit the effects of underexpression as well as overexpression.
  • antisense downregulation For underexpression there are two principle methods which are commonly referred to in the art as “antisense downregulation” and “sense downregulation, “cosuppression” or ''gene silencing". Both of these methods lead to an inhibition of expression of the target gene. Other lesser used methods involve modification of the genetic control elements, the promoter and control sequences, to achieve greater or lesser expression of an inserted gene.
  • the DNA is inserted by injection into individual cells via an ultrafme hollow needle.
  • Another method viz. fibre-mediated transformation, applicable to both monocots and dicots, involves creating a suspension of the target cells in a liquid, adding microscopic needle-like material, such as silicon carbide or silicon nitride "whiskers", and agitating so that the cells and whiskers collide and DNA present in the liquid enters the cell.
  • One suitable application of the present invention is the modulation of ripening and/or senescence processes in banana.
  • Bananas are a globally important fruit crop. They are not only a popular dessert fruit, but represent a vital carbohydrate staple in the tropics with as many as 100 million people subsisting on bananas and plantains as their main energy source.
  • the cultivated dessert banana is commonly triploid, parthenocarpic and belongs to the musa AAA genome group, eg. Cavendish subtypes.
  • Bananas are climacteric fruits and ripening is regulated by ethylene produced by the fruit and involves numerous biochemical changes including the conversion of starch to sugars, cell wall disassembly, synthesis of volatile compounds, changes in phenolic constituents and degradation of chlorophyll in the peel.
  • a method of modulating the fruit ripening or tissue senescence characteristics of a plant of the genus Musa comprising inserting into the genome of said plant a DNA construct comprising in sequence a promoter region which is operable in plant cells, a DNA having a nucleotide sequence selected from SEQ ID Nos. 1-73, complementary sequences of SEQ ID Nos. 1-73 and variants of said sequences permitted by degeneracy of the genetic code and a transcription termination sequence, and selecting from the population of regenerants those transformants with modulated fruit ripening or tissue senescence characteristics.
  • the invention also provides a method as described above wherein the said DNA insert comprises a full length polynucleotide coding sequence which includes a polynucleotide sequence as shown in any one of SEQ ID Nos. 1-73.
  • the promoter of the said DNA construct may be constitutive, developmentally regulated, or switchable. It may additionally be tissue specific or organ specific.
  • the promoter may specifically be either the SAG 1 promoter, the polyubiquitin promoter or the banana ACC oxidase promoter.
  • Suitable transformation methods for use with the present invention include the Agrobacterium, microparticle bombardment, fibre mediated or direct insertion methods.
  • the invention further provides plant material, plants, their progeny and seed produced according to a method as described above characterised in that said plant material and plants exhibit modulated ripening or tissue senescence characteristics.
  • the gene sequences of the present invention may be synthesised ab initio, using the sequence data provided in the sequence listing provided herewith, or isolated from a library using the standard techniques know within the art. To assist the isolation of these polynucleotides we have deposited with the National Collection of Industrial & Marine Bacteria, St. Machar Drive, Aberdeen, UK, a cDNA library of the banana peel ripening related genes. The library was deposited on 9th July 1996 and has the Accession Number 40813.
  • this invention is based on the identification of genes which encode proteins involved in banana ripening-related processes, specifically within banana peel.
  • the DNA sequences may be used in the process of modifying the plant ripening characteristics of plants and/or fruit.
  • banana plants can be generated which, amongst other phenotypic modifications, may have one or more of the following fruit characteristics: improved resistance to damage during harvest, packaging and transportation due to slowing of the ripening and over-ripening processes; longer shelf life and better storage characteristics due to reduced activity of degradative pathways (e.g.
  • DNA constructs according to the invention may comprise a base sequence at least 10 bases (preferably at least 35 bases) in length for transcription into RNA. There is no theoretical upper limit to the base sequence - it may be as long as the relevant mRNA produced by the cell - but for convenience it will generally be found suitable to use sequences between 100 and 1000 bases in length. The preparation of such constructs is described in more detail below.
  • a suitable cDNA or genomic DNA or synthetic polynucleotide may be used as a source of the DNA base sequence for transcription.
  • the isolation of suitable ripening- related sequences is described above; it is convenient to use DNA sequences derived from the ripening-related clones deposited at NCIMB in Aberdeen. Sequences coding for the whole, or substantially the whole, of the appropriate ripening-related protein may thus be obtained. Suitable lengths of this DNA sequence may be cut out for use by means of restriction enzymes.
  • genomic DNA as the source of a base sequence for transcription it is possible to use either intron or exon regions or a combination of both.
  • the cDNA sequence as found in one of the banana plasmids or the gene sequence as found in the chromosome of the banana plant may be used.
  • Recombinant DNA constructs may be made using standard techniques.
  • modulation means either an increase or decrease. More specifically “modulation the ripening or tissue senescence process in plants” means an alteration being either an increase or decrease in the said processes relative to an untreated or transformed plant.
  • “Senescence” means the progressive deterioration in function of cells, tissues, organs etc., related to the period of time since that function commenced.
  • Plant material includes plant cells and any other type of plant regenerable material.
  • Frull length polynucleotide coding sequence includes a polynucleotide coding for the whole or substantially the whole of the appropriate ripening related mRNA/protein.
  • TABLE is a list of all the clones isolated from banana peel and the corresponding sequence identity number as provided in the sequence listing herein.
  • the table also illustrates the approximate clone size, the percentage identity and nucleotide similarity based on the results obtained from comparisons with the EMBL sequence database.
  • the table provides the putative gene identity based on these comparisons, corresponding published sequences and their database accession numbers.
  • FIGURE 1 Plant transformation vector pUN, containing the UBI polyubiquitin promoter.
  • FIGURE 2 Plant transformation vector pSHYN, containing hygromycin resistance gene for selection of transformed plants.
  • FIGURE 3 Plant transformation vector pFAN, containing the banana ACC oxidase promoter.
  • the first and second strands of the cDNAs were synthesised from the messenger RNAs using a commercial cDNA synthesis kit (Catalog No. 200450, ZAP ExpressTM Gold Cloning kit, Stratagene Ltd, Cambridge, Cambs, UK). Double stranded cDNAs were cloned into the ZAP ExpressTM vector, packaged, mixed with plating bacteria to determine titre and for library screening, following instructions of the suppliers protocol.
  • the unamplified cDNA library from ripening banana peel was differentially screened using cDNA from unripe and ripening banana peel tissue.
  • a proportion of the library was plated individually at low density and duplicate plaque lifts made onto Hybond N nylon filters (Amersham) according to the manufacturer's instructions.
  • One filter was hybridised to dCTP radiolabeled cDNA from green fruit and the duplicate filter hybridised to dCTP radiolabeled cDNA from ripening fruit.
  • Hybridisations were at high stringency. Plaques hybridising preferentially with ripening or green radiolabeled cDNA were picked and replated for a second round of selection by differential screening. These clones were numbered as ripening up- or down-regulated peel clones.
  • the clones were in-vivo excised from the ZAP expressTM vector into the pBK- CMV phagemid vector using the ExAssistTM interference-resistant helper phage, following instructions from manufacturers protocol. 1.4 Characterisation of the ripening peel cDNA library and the ripening-related clones.
  • the ripening cDNA library from peel tissue were prepared with an efficiency of 3.2 x 10 5 plaque-forming units per microgram of cDNA.
  • the sizes of the inserts in the peel library was 0.4 - 6.7 Kb with a mean size insert of 1.47 Kb.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 73. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992,
  • the partial sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig. 2.) which has been cut with Kpnl and the ends made flush with Klenow polymerase.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants.
  • a vector is constructed using the sequences corresponding to a fragment of the inserts of one of the sequences 1 to 73. This fragment is synthesised by polymerase chain reaction using synthetic primers incorporating BamHI restriction sites suitable for cloning between a maize UBI polyubiquitin promoter (Christensen et al, 1992, Plant Molecular Biology, 18:675-689) and a nopaline synthase 3'end termination sequences in the vector pFAN ( Figure 3.) The truncated sense expression cassette is excised by digestion with Ascl, the ends of the fragment are made flush with T4 polymerase and it is cloned into the vector pSHYN (Fig.
  • pSHYN contains hygromycin resistance gene for selection of transformed plants. After synthesis of the vector, the structure and orientation of the sequences are confirmed by DNA sequence analysis.
  • EXAMPLE 4 Construction of an over-expression vector with the maize polyubiquitin promoter. The complete sequence of a ripening related cDNA containing a full open-reading frame is inserted into the vectors described in EXAMPLE 2.
  • Transformed Musa plants containing the vectors are produced by the method described in Sagi et al. (1995) Biotechnology. Vol. 13 pp 481-485. Regenerated transformed plants are identified by their ability to grow on hygromycin and grown to maturity. Ripening fruit are analysed for a modulation in their ripening related or senescence characteristics.
  • TCCTGCGTCA TCTCCCCGTC TCCAACATTT CTGAGATCCC CGATCTTGAT GACCAGTATA 360
  • GCTCCTC 787 INFORMATION FOR SEQ ID NO: 4:
  • CAGCGTCCAC ATCTCCGGCG GGCCGTGCAT CACCATCCAG TACGTCACCA ACATCNTCAT 540 CCACGGCGTC CACATCCACN ACTGCAAGCA GGGCGGGAAC GCGTTNCGTG CGCGACNCCC 600
  • CCAGCGTCCA CATCTCCGGC GGGCCGTGCA TCACCATCCA GTTACTTTCN CCAACATCAT 540
  • CAGGGCACTA CGGGTTGGCG CNCGGTGTCN GACGGCGACG GGGTTTCCAT CTTCGGCGGG 660
  • GCTCATCATG AACTCCCACA AGACCATCCN ACNGCCGGGG CGCCAGCGTC CNCNTCTCCG 480
  • AACATCCACN AACTGCNANC TNGGCGGGGA AANNNTTTCG TTNCCGCNAC TCCCCAAGGG 600
  • ACATCATCAT CCACGGCGTC CACATCCACG ACTGCAANCA GGGCGGGAAC GCGTACGTGC 540
  • GCGACTCCCC AAGGCACTNC GGGTTGGCNC ACGGTGTCCG ACGGCNACGG GGTGTCCATC 600
  • TCCCCCAGGG CNCTNACGGG TTGGCCACGG TGTTCGGACG GCAACGGGGT NTTCATCTTC 660
  • TCTACTCTTC TCGTGCACCC TTAACGTCAG CAGCTGTCCG CGACCCTGAA TTAGTAGTAC 120
  • CAGGGCACTA CGGGTTGGCG CACGGTGTCG GACGGCNAAN GGGTTTCCAT CTTTCNGCNG 660
  • GAAGCATCGC AACGACGCAG CCTGCCCCGG CAAGGGCTTC TACACGTACA CCGCCTTCAT 180
  • GCCCCATCCA AATCTCATTC AACTACAACT ACGGGGCCGG CCGGGAANAG CCATCGGCTC 480
  • GAAGCATCGC AACGACGCAN CCTGCCCCGG CAAGGGCTTC TACACGTACA ACGCCTTCAT 300
  • CTACTGCGTC NCCAGCTCGC ANTGGCCGTG CCCTGCACGC ANTAATTACT TNCGGCCGAA 540
  • GCCCCATCCA AATCTCATTC AACTACAACT ACNGGCCCGG CCGGGAAAAC CAATCGGCTC 600
  • GTGCGGTAAC ACGGATCCAT ACTGCGGCCA AGGATGCCAN AGCCAATGCA CAGGCTCCAC 180
  • TTNCACCGCC TTCATCGCCG CCGCCAACTC CTTCAGCGGG TTCGGGACGA CCGGCGACNA 360
  • AACCCGANCC ATTCTTCCTT TCAAAAACGG CTCTTTTGGT TCCGGNATAA ATNCCTCCAT 720
  • NNTCCAAACC 730 (2) INFORMATION FOR SEQ ID NO: 27:
  • GTGCGGTAAC ACGGATCCAT ACTGCGGCCA AGGATGCCAG AGCCAATGCA CAGGCTCCAC 180
  • AAACCATCNG CTTCCGACTG CTTCAACAAC CCAAACTTGG TTGGCCACCN AACCCGAACC 660
  • TCCTTGCCNC AAANTTNATA ACCCGGGAAC TTGGACCCCC TCCAACCC 768
  • TGTACCCTTA TTTTAGCTAC ACCGGCAACC CGGGACAGAT CTCGCTGCCC TACGCCCTGT 360
  • CAGCGTCTCC TTTCGATACA TAGCTGTCGG AAACGAGCTG ATCCCCGGAT CGGATCTGGC 420 GCAGTACNTC CTCCCCGCCA TGCGCAACAT CTACAATGCT TTGTCCTCGG CTGGCCTGCA 480
  • AAGGANATCN AGGCTTTCAT TTTCCAANAT TTCAACCAAA AACCNANNGG TTGGAAGGAT 660
  • CTTTGGCGAN TAACGGAACG CCGCTCCTGG TCAATGTTGT TNCCTTATTT TTAGCTACNC 660
  • GTGTCTGCTA CGGAATGCTC GGCAACAATC TTCCCCCGCC CAGCGAGGTG GTCAGTCTCT 180
  • GCGCAGTACA TCCTCCCCGC CATGCGCAAC ATCTACAATG CTTTGTCCTC GGCTGGCCTG 480
  • TGGTGTCTGC TACGGCATGC TCGGCAACAA TCTTCCCCCG CCCAGCGAGG TGGTCAGTCT 180
  • CAGTCTCTAC AAATCCAACA AATCGCGAGG ATGAGACTCT ACGATCCAAA CCAAGCCGCC 240
  • CTGCAAGCCC TCAGGAACTC CAACATCCAA GTCCTGTTGG ATGTCCCCCG ATNCNACGTG 300
  • GCAAGCCCTC AGGAACTCCA ACATCCAAGT CCTGTTGGAT GTCCCCCGAT CCGACGTGCA 300
  • GCTTCANCGA ATTTGGGGAT CNAATCNAGC ACTGGATCAC CGTCAATGAA CCCAACATAN 600
  • CACGAGGCAG AATTGTCTCC CACGCTGAGC AAGAGCATCT TTGAAGGAGC CGGTGGATCT 60
  • TCAGANCATC CCGAGCTGAC TGCGAAGGGA TAGTCGTNAA CGCTGANCGC GTNNCGGCAT 600
  • ACAGCTGTGC G ACCGGGCGA CATCGCCTAC TTCTTCTTGG CAGGATCCCT AGGAGTGCTC 300

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Abstract

La présente invention concerne un procédé permettant de moduler les caractéristiques de maturation et/ou de sénescence chez des végétaux du genre Musa. Ce procédé consiste à transformer ces végétaux au moyen d'une ou plusieurs séquences disponibles à partir de l'échantillothèque d'ADNc déposée et dont le numéro d'accession est 40183, puis à régénérer les végétaux considérés, et à sélectionner dans la population de transformants, ceux des végétaux présentant des caractéristiques de modulation et/ou de sénescence tissulaire.
PCT/GB1998/001297 1997-05-20 1998-05-05 Maturation des fruits par genie genetique WO1998053085A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU72257/98A AU7225798A (en) 1997-05-20 1998-05-05 Genetic control of fruit ripening
US09/949,052 US20020026657A1 (en) 1997-05-20 2001-09-07 Genetic control of fruit ripening

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9710370.9A GB9710370D0 (en) 1997-05-20 1997-05-20 Genetic control of fruit ripening
GB9710370.9 1997-05-20

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WO1998053085A1 true WO1998053085A1 (fr) 1998-11-26

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WO (1) WO1998053085A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015668A3 (fr) * 1997-09-25 1999-10-07 Thompson Boyce Plant Res Proteines de banane, adn, et elements regulateurs d'adn associes au developpement du fruit
WO1999053072A1 (fr) * 1998-04-09 1999-10-21 E.I. Du Pont De Nemours And Company Homologues de la proteine r1 de phosphorylation de l'amidon
WO2000001804A3 (fr) * 1998-07-03 2000-02-24 Unilever Nv Sequence nucleotidique
WO2000000619A3 (fr) * 1998-06-26 2000-06-15 Univ Iowa State Res Found Inc MATERIAUX ET PROCEDES PERMETTANT D'ALTERER LES NIVEAUX D'ENZYMES ET D'ACETYLE CoA CHEZ LES PLANTES
US6620987B1 (en) 1998-04-09 2003-09-16 E. I. Dupont De Nemours & Company Nucleic acid encoding a starch R1 phosphorylation protein homolog from maize

Citations (3)

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WO1991001375A1 (fr) * 1989-07-14 1991-02-07 Imperial Chemical Industries Plc Structures d'adn, cellules et plantes derivees de celles-ci
WO1992012249A1 (fr) * 1990-12-26 1992-07-23 Monsanto Company Regulation du murissement des fruits et de la senescence chez les plantes
WO1995015678A1 (fr) * 1993-12-09 1995-06-15 The Texas A & M University System TRANSFORMATION D'ESPECES MUSA AU MOYEN $i(D'AGROBACTERIUM TUMEFACIENS)

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WO1991001375A1 (fr) * 1989-07-14 1991-02-07 Imperial Chemical Industries Plc Structures d'adn, cellules et plantes derivees de celles-ci
WO1992012249A1 (fr) * 1990-12-26 1992-07-23 Monsanto Company Regulation du murissement des fruits et de la senescence chez les plantes
WO1995015678A1 (fr) * 1993-12-09 1995-06-15 The Texas A & M University System TRANSFORMATION D'ESPECES MUSA AU MOYEN $i(D'AGROBACTERIUM TUMEFACIENS)

Non-Patent Citations (8)

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Title
CLENDENNEN S K ET AL: "ISOLATION AND IDENTIFICATION OF GENES DIFFERENTIALLY EXPRESSED DURING BANANA FRUIT RIPENING", PLANT PHYSIOLOGY, vol. 111, no. 2, June 1996 (1996-06-01), pages 34, XP002049413 *
DOMINGUEZ-PUIGJANER E ET AL: "DIFFERENTIAL PROTEIN ACCUMULATION IN BANANA FRUIT DURING RIPENING", PLANT PHYSIOLOGY, vol. 98, no. 1, January 1992 (1992-01-01), pages 157 - 162, XP002049414 *
HUANG P -L ET AL: "CHARACTERIZATION AND EXPRESSION ANALYSIS OF A BANANA GENE ENCODING 1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 41, no. 5, April 1997 (1997-04-01), pages 941 - 950, XP000675954 *
LOPEZ-GOMEZ R ET AL: "ETHYLENE BIOSYNTHESIS IN BANANA FRUIT: ISOLATION OF A GENOMIC CLONE TO ACC OXIDASE AND EXPRESSION STUDIES", PLANT SCIENCE, vol. 123, no. 1/02, 1997, pages 123 - 131, XP000676021 *
MEDINA-SUAREZ R ET AL: "GENE EXPRESSION IN BANANA PEEL AND PULP DURING RIPENING", PLANT PHYSIOLOGY, vol. 111, no. 2, June 1996 (1996-06-01), pages 122, XP002049412 *
MEDINA-SUAREZ R ET AL: "GENE EXPRESSION IN THE PULP OF RIPENING BANANAS1 TWO-DIMENSIONAL SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS OF IN VITRO TRANSLATION PRODUCTS AND CDNA CLONING OF 25 DIFFERENT RIPENING-RELATED MRNAS", PLANT PHYSIOLOGY, vol. 115, no. 2, October 1997 (1997-10-01), pages 453 - 461, XP002049416 *
SAGI, L., ET AL.: "GENETIC TRANSFORMATION OF BANANA AND PLANTAIN (Musa spp.) VIA PARTICLE BOMBARDMENT", BIOTECHNOLOGY, vol. 13, May 1995 (1995-05-01), pages 481 - 485, XP002073183 *
SEYMOUR G B: "BANANA", BIOCHEMISTRY OF FRUIT RIPENING, 1993, pages 83 - 106, XP002049415 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999015668A3 (fr) * 1997-09-25 1999-10-07 Thompson Boyce Plant Res Proteines de banane, adn, et elements regulateurs d'adn associes au developpement du fruit
US6284946B1 (en) 1997-09-25 2001-09-04 Boyce Thompson Institute For Plant Research Inc. Banana DNA associated with fruit development
WO1999053072A1 (fr) * 1998-04-09 1999-10-21 E.I. Du Pont De Nemours And Company Homologues de la proteine r1 de phosphorylation de l'amidon
US6620987B1 (en) 1998-04-09 2003-09-16 E. I. Dupont De Nemours & Company Nucleic acid encoding a starch R1 phosphorylation protein homolog from maize
WO2000000619A3 (fr) * 1998-06-26 2000-06-15 Univ Iowa State Res Found Inc MATERIAUX ET PROCEDES PERMETTANT D'ALTERER LES NIVEAUX D'ENZYMES ET D'ACETYLE CoA CHEZ LES PLANTES
US6764851B2 (en) 1998-06-26 2004-07-20 Iowa State University Research Foundation, Inc. Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
US6942994B2 (en) 1998-06-26 2005-09-13 Iowa State University Research Foundation, Inc. Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
WO2000001804A3 (fr) * 1998-07-03 2000-02-24 Unilever Nv Sequence nucleotidique

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GB9710370D0 (en) 1997-07-16

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