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WO1998047599A1 - Tube de filtration, notamment de microspheres - Google Patents

Tube de filtration, notamment de microspheres

Info

Publication number
WO1998047599A1
WO1998047599A1 PCT/EP1998/002293 EP9802293W WO9847599A1 WO 1998047599 A1 WO1998047599 A1 WO 1998047599A1 EP 9802293 W EP9802293 W EP 9802293W WO 9847599 A1 WO9847599 A1 WO 9847599A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
fabric
pipe according
microspheres
mesh size
Prior art date
Application number
PCT/EP1998/002293
Other languages
German (de)
English (en)
Inventor
Anthony G. Harris
Eckart Thein
Stephan Raab
Original Assignee
Harris Anthony G
Eckart Thein
Stephan Raab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harris Anthony G, Eckart Thein, Stephan Raab filed Critical Harris Anthony G
Priority to AU75272/98A priority Critical patent/AU7527298A/en
Publication of WO1998047599A1 publication Critical patent/WO1998047599A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/087—Single membrane modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/08—Filter cloth, i.e. woven, knitted or interlaced material
    • B01D39/083—Filter cloth, i.e. woven, knitted or interlaced material of organic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/18—Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/081—Manufacturing thereof
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16L—PIPES; JOINTS OR FITTINGS FOR PIPES; SUPPORTS FOR PIPES, CABLES OR PROTECTIVE TUBING; MEANS FOR THERMAL INSULATION IN GENERAL
    • F16L9/00—Rigid pipes
    • F16L9/12—Rigid pipes of plastics with or without reinforcement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • B01D2239/06—Filter cloth, e.g. knitted, woven non-woven; self-supported material
    • B01D2239/065—More than one layer present in the filtering material
    • B01D2239/0672—The layers being joined by welding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • B01D2239/06—Filter cloth, e.g. knitted, woven non-woven; self-supported material
    • B01D2239/065—More than one layer present in the filtering material
    • B01D2239/0681—The layers being joined by gluing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • B01D2239/12—Special parameters characterising the filtering material
    • B01D2239/1216—Pore size

Definitions

  • the invention relates to a tube for filtration, in particular of microspheres, and the use of a fabric for the filtration of microspheres.
  • the invention is technically usable in particular in the field of basic research in medicine and geology.
  • Particular uses are, for example, the filtration of blood and generally of hydrophilic and hydrophobic liquids, in particular paraffin oil and silicone oil.
  • the basic substance for the microspheres is preferably polystyrene, to which the corresponding dye or fluorescent substance has been added, the diameter of the beads being able to vary between approximately 1 ⁇ m and 15 ⁇ m depending on the field of application. Tissue samples from certain organs are usually taken for an examination, in which the deposition of the microspheres in question is then examined. Since the related dye or fluorescent dye is unstable to numerous chemicals and the surrounding pH environment, the microspheres must be completely isolated from the surrounding reaction liquid, such as the tissue in solution, before the dyes are extracted.
  • the object of the present invention is therefore to improve such examination methods.
  • a tube for filtration in particular of microspheres, which is characterized in that, over a cross section of the tube, a woven fabric made of polyamide, Teflon, polypropylene, polystyrene, glass fibers and / or stainless steel with a mesh size of 1 ⁇ m to 15 ⁇ m is stretched.
  • a tube makes it possible in an examination in which the tissue substance has been dissolved using a chemical reaction, for example by adding potassium hydroxide, without changing the microspheres in their substance and in particular with regard to the addition of dye and fluorescent substance to separate the tissue in solution from the microspheres by filtration.
  • a tube it is of course also possible to use another vessel with an outlet opening to which the tissue in question is attached.
  • the shape of the vessel should only be designed in such a way that, when possible, all microspheres collect on the filter during the filtration.
  • a vacuum can also be applied to the opening, in which case it may be expedient that the further openings of the vessel or tube can be closed in a vacuum-tight manner.
  • microspheres most commonly used at the moment are practically made of a material that acts as a sponge, so to speak, in which the dye is absorbed.
  • the dye can then be removed from the microspheres by adding solvent from the microspheres.
  • the solvent with the dye dissolved in it is finally separated from the remaining bodies of the microspheres by filtration and collected.
  • the solvent with the dye contained therein is finally subjected to an optical examination.
  • a major advantage of the invention is that both the process of separating the microspheres still containing the dye from the reaction liquid still containing the dissolved organic tissue and the further subsequent step of dissolving the Dye from the microspheres and separation of this solution from the microspheres retained by the filtration tissue can be carried out in one and the same filter vessel. This ensures that no microspheres are actually lost in the individual process operations, so that a quantitative measurement is ensured.
  • the tissue is detachable and tightly connected to the inner wall of the tube by pressing in a snap ring. This enables the filter fabric to be changed easily.
  • the fabric is held stretched over one end of the tube and releasably secured on the outside of the tube.
  • the holder on the outside of the tube can be made using a clamp.
  • the fabric can also be permanently and sealingly attached to the edge of one end of the tube.
  • the pipe is preferably made of a plastic material for reasons of chemical corrosion resistance
  • the attachment of the fabric to the pipe by means of thermal welding, ultrasonic welding or by gluing with the aid of an adhesive has proven particularly useful.
  • the edge of the fabric is cast into the tube during manufacture.
  • the task set out in the beginning is also achieved overall by using a woven fabric made of polyamide, Teflon, polypropylene, polystyrene, glass fibers and / or stainless steel with a mesh size of 1 ⁇ m to 15 ⁇ m for the filtration of microspheres.
  • a woven fabric made of polyamide, Teflon, polypropylene, polystyrene, glass fibers and / or stainless steel with a mesh size of 1 ⁇ m to 15 ⁇ m for the filtration of microspheres.
  • 1 is a schematic view of a pipe in longitudinal view with an indication of the pipe cross section
  • Fig. 2 is a schematic representation of the tube shown in Fig. 1 in a perspective view
  • FIG. 3 shows a further embodiment of a tube designed according to the invention in a view similar to that in FIG. 1.
  • the tube 1 shown in Fig. 1 consists of a tube part which is slightly conical from top to bottom in Fig. 1, although this is not explicitly shown.
  • the tube also has a flange 2 attached to its outer upper end, which has a thickness of approximately 1, 20 mm and an outer diameter of approximately 29.0 mm.
  • the tube 1 has a circular cross-section overall, although other cross-sectional shapes are also possible.
  • a fabric 4 is stretched over the entire cross-sectional opening of the tube, which is permanently and sealingly connected to the tube on the entire circumference of the lower edge 3.
  • the fabric can be woven from monofilament polyamide threads and can have different mesh sizes, such as:
  • the polyamide fabric can be permanently attached to the lower edge 3 of the tube 1 with the aid of ultrasound welding or thermal welding or also by gluing with a suitable adhesive.
  • the polyamide fabric can also be attached to the tube by placing it in the tube injection mold prior to an injection molding process so that it is firmly molded into the tube at its edge.
  • connection of the fabric 4 with the lower edge 3 of the tube should, if possible, be so designed and constructed that suction of the liquid in the tube by means of negative pressure, cleaning by means of rinsing, application of an ultrasonic bath, and multiple use of the filtration tubes, the Do not negatively affect the connection point and the tissue.
  • a filtration tube 1 is preferably used in combination with suitable filter holders which have a removable closure container.
  • the reaction liquid in which the microspheres are included are added, wherein the reaction liquid from about consist chemically solubilized tissue 'can, in which the microspheres were contained previously.
  • the microspheres are then separated from the reaction liquid by filtration, the microspheres being retained in the tube, while the reaction liquid is drawn in, for example, by means of a negative pressure applied to the lower end of the tube.
  • a quantitative evaluation of the number of the microspheres can then be carried out by dissolving them with a suitable organic solvent, whereby the dyes or fluorescent substances contained in the microspheres are released.
  • FIG. 3 shows a filtration tube 11 with the same dimensions as in FIG. 1.
  • the difference from the tube shown in Fig. 1 consists essentially in the fact that at the lower end 15 of the tube 11, a grating 16 is used which bears against the inner wall of the tube and which consists approximately of polypropylene and has a mesh size 17 of 0.5 mm .
  • a polyamide fabric 17 is placed on the inside of the tube 16 facing the inside of the tube 11 and is attached to its edge 18 at the edge by means of ultrasound welding or thermal welding or gluing to the inside of the tube 11.
  • the polyamide fabric can also consist of another fabric of the materials already mentioned above.
  • the fabric 17 can also, which is not shown in FIG. 3, be arranged on the underside of the grid 16 and then be permanently and sealingly connected to the lower edge 19 at the edge.
  • the same connection methods as described above can be used here.
  • the tube 11 has a similar appearance as the tube shown in FIG. 2.

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • General Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Manufacturing & Machinery (AREA)
  • Mechanical Engineering (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Manufacturing Of Micro-Capsules (AREA)

Abstract

L'invention concerne un dispositif filtrant pour séparer des microsphères contenues dans des échantillons tissulaires. Pour séparer les microsphères contenues dans les échantillons tissulaires parvenus en solution, il est prévu d'utiliser un filtre réalisé dans un tissu en polyamide, en Téflon, en polypropylène, en polystyrène, en fibres de verre et/ou en acier, avec une largeur de maille comprise entre 1 et 15 mu m.
PCT/EP1998/002293 1997-04-18 1998-04-17 Tube de filtration, notamment de microspheres WO1998047599A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75272/98A AU7527298A (en) 1997-04-18 1998-04-17 Filter tube, specially for microspheres

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE29706998U DE29706998U1 (de) 1997-04-18 1997-04-18 Rohr zur Filtration von Mikrosphären mittels Geweben mit einer porengröße von 1mum bis 15mum
DE29706998.5 1997-04-18

Publications (1)

Publication Number Publication Date
WO1998047599A1 true WO1998047599A1 (fr) 1998-10-29

Family

ID=8039186

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1998/002293 WO1998047599A1 (fr) 1997-04-18 1998-04-17 Tube de filtration, notamment de microspheres

Country Status (3)

Country Link
AU (1) AU7527298A (fr)
DE (1) DE29706998U1 (fr)
WO (1) WO1998047599A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1029088A (fr) * 1950-12-05 1953-05-29 Monture de filtre ou tamis amovible
EP0396008A2 (fr) * 1989-04-29 1990-11-07 Leybold Aktiengesellschaft Pompe à vide avec filtre d'aspiration
EP0408462A2 (fr) * 1989-07-14 1991-01-16 Terumo Kabushiki Kaisha Matériau filtrant d'interception de leucocytes et méthode de sa fabrication
WO1996020035A1 (fr) * 1994-12-23 1996-07-04 Baxter International Inc. Procede de filtrage des leucocytes a partir de sang frais
EP0729774A2 (fr) * 1995-03-03 1996-09-04 Hoechst Aktiengesellschaft Article façonné ayant un dispositif de filtration

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1029088A (fr) * 1950-12-05 1953-05-29 Monture de filtre ou tamis amovible
EP0396008A2 (fr) * 1989-04-29 1990-11-07 Leybold Aktiengesellschaft Pompe à vide avec filtre d'aspiration
EP0408462A2 (fr) * 1989-07-14 1991-01-16 Terumo Kabushiki Kaisha Matériau filtrant d'interception de leucocytes et méthode de sa fabrication
WO1996020035A1 (fr) * 1994-12-23 1996-07-04 Baxter International Inc. Procede de filtrage des leucocytes a partir de sang frais
EP0729774A2 (fr) * 1995-03-03 1996-09-04 Hoechst Aktiengesellschaft Article façonné ayant un dispositif de filtration

Also Published As

Publication number Publication date
AU7527298A (en) 1998-11-13
DE29706998U1 (de) 1997-08-14

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