WO1998040750A1 - Determination du pourcentage d'hemoglobine glycosylee - Google Patents
Determination du pourcentage d'hemoglobine glycosylee Download PDFInfo
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- WO1998040750A1 WO1998040750A1 PCT/US1998/005005 US9805005W WO9840750A1 WO 1998040750 A1 WO1998040750 A1 WO 1998040750A1 US 9805005 W US9805005 W US 9805005W WO 9840750 A1 WO9840750 A1 WO 9840750A1
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- Prior art keywords
- ghb
- hemoglobin
- microparticles
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- solid phase
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
- G01N2474/20—Immunohistochemistry assay
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/824—Immunological separation techniques
Definitions
- the present invention relates to a method for detecting the presence or amount of glycated hemoglobin (GHb) in a blood sample, and more particularly, to an improved, highly accurate, one-read method for assessing percentage glycated hemoglobin, that is, a method which does not require a measurement of total hemoglobin (THb) .
- GHb glycated hemoglobin
- Glycated hemoglobin refers to a series of minor hemoglobin components that are formed via the attachment of various sugars (most commonly glucose) to the hemoglobin molecule.
- the human erythrocyte is freely permeable to glucose. Within each erythrocyte, GHb is formed at a rate that is directly proportional to the ambient glucose concentration.
- the reaction of glucose with hemoglobin is nonenzymatic, irreversible and slow, so that only a fraction of the total hemoglobin is glycated during the life span of an erythrocyte (120 days) .
- the measurement of GHb provides a weighted "moving" average of blood glucose levels that can be used to monitor long- term blood glucose levels, providing an accurate index of the mean blood glucose concentration over the preceding 2 to 3 months.
- the most important clinical application of this is in the assessment of glycemic control in a diabetic patient .
- Hemoglobin Ale is one specific type of glycated hemoglobin and is the most important hemoglobin species with respect to diabetes.
- the amount of total hemoglobin that is HbAlc is approximately 3 to 6% in nondiabetics, and 20% or greater in diabetes that is poorly controlled (Goldstein DE, et al, Clin Chem 32 : B64-B70 (1986) ) .
- glucose is attached to the amino terminal valine residue of one or both of the hemoglobin A beta chains.
- HbAlc (as well as other glycated Hemoglobin Al ⁇ species) can be separated from nonglycated hemoglobins by methods that separate molecules based on differences in their electrical charges.
- HbAO glycation of hemoglobin also occurs at other sites on the hemoglobin molecule, but these species cannot be separated from nonglycated hemoglobins based on charge differences, so all of these species of hemoglobin are termed HbAO .
- Methods that measure all forms of glycated hemoglobin are said to measure total GHb. Since glycation at one site appears to be proportional to glycation at any other site, there is a linear relationship between GHb and HbAlc.
- DCCT Diabetes Control and Complications Trial
- GHb Standardization Subcommittee recommended that within- laboratory, between-run CVs be maintained at 5% or lower for all GHb assays, and that standardization be based on correlation to the DCCT for fresh samples. All manufactured assays must meet these requirements to receive certification.
- Clinical assay methods separate GHb from total - - hemoglobin based on either charge differences or structural characteristics. Methods based on charge differences include cation exchange chromatography and electrophoresis, and separate HbAl or HbAlc from HbAO based on the difference in their charges. Ion exchange chromatography can be performed either in large columns, mini or micro columns, or by high pressure liquid chromatography (HPLC) .
- Methods based on structural characteristics include affinity binding or chromatography and immunoassays . These methods are less sensitive to small variations in temperature, pH or ionic strength, and generally are not affected by labile GHb intermediates, hemoglobinopathies or sample storage conditions or the extraneous clinical factors mentioned above. However these methods either involve separation of GHb from nonglycated components or require 2 separate determinations - one for total hemoglobin and a second for GHb or HbAlc - to calculate %GHb.
- Use of a boronate ligand coupled to a solid phase matrix can be used in affinity binding assays due to the affinity of boronate for GHb.
- Ratios of bound (glycated) to nonbound (nonglycated) hemoglobin can then be quantified.
- Immunoassays measure HbAlc using HbAlc specific antibodies, but require 2 separate determinations, one for " total hemoglobin and the other for HbAlc, in order to calculate %GHb.
- an immunoassay may bind all hemoglobin species using passive adsorption, then detect HbAlc with a specific antibody conjugate; however this method may be adversely affected by hemoglobin variants.
- the unique and novel approach described herein measures %GHb in whole blood samples and provides a %GHb result following only a single measurement. This differs from the methods described above and currently available in the art which require separate total and GHb measurements to calculate a %GHb result as, for example, a ratio of GHb/THb X 100.
- the GHb assay described herein uses a simple procedure for the determination of DCCT Standardized %GHb in whole blood samples. First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic, phenylboronic or boric acid or related boronate compound through covalent linkage chemistries known in the art .
- This solid phase is novel since it specifically captures GHb in direct proportion to the %GHb in the sample.
- a labeled component that recognizes human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample.
- the advantages of measuring %GHb using a single determination include high precision (less than about 5% CVs) and, since the assay is easily automatable, high throughput (100 to 200 tests/hour) . With automation, this assay can also be consolidated with other testing on one analyzer.
- Figure 1 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using CMA/Merquat coated particles and samples pre-mixed with polyanion reagent according to one embodiment of the invention.
- Figure 2 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using CMA/Merquat coated particles with polyanion reagent according to one embodiment of the invention.
- Figure 3 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using CMA-APBA coated particles according to one embodiment of the invention.
- Figure 4 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using CMC-APBA coated particles according to one embodiment of the invention.
- Figure 5 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using PAA-APBA coated particles according to one embodiment of the invention.
- Figure 6 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using TREN-CMA-APBA coated particles according to one embodiment of the invention.
- Figure 7 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using TREN-CMC-APBA coated particles according to one embodiment of the invention.
- Figure 8 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using APBA coated particles according to one embodiment of the invention.
- Figure 8A is a graph illustrating the results of testing whole blood samples to determine %GHb levels using EDA-CMA-APBA coated particles according to one embodiment of the invention.
- Figure 9 is a graph illustrating the correlation between %GHb levels determined using the Biorad Diamat HPLC and the preferred embodiment of the invention.
- Figure 10 is a graph illustrating the correlation between %GHb levels determined using the Abbott IMx ® GHb assay and the preferred embodiment of the invention.
- Figure 11 is a graph illustrating a run-to-run correlation when determining %GHb levels using the preferred embodiment of the invention.
- Figure 12 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using a one-step (simultaneous) format according to one embodiment of the invention.
- Figure 13 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using Cortex polyclonal goat anti-human hemoglobin labeled with acridinium according to one embodiment of the invention.
- Figure 14 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using Bios Pacific monoclonal anti-human hemoglobin labeled with acridinium according to one embodiment of the invention.
- Figure 15 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using Bios Pacific polyclonal goat anti-human hemoglobin labeled with acridinium according to one embodiment of the invention.
- Figure 16 is a graph illustrating the results of testing whole blood samples to determine %GHb levels using Dako polyclonal rabbit anti-human hemoglobin labeled with acridinium according to one embodiment of the invention.
- Per cent glycated hemoglobin is determined following hemolysis of the red blood cells in whole blood to release hemoglobin, after which samples are diluted and incubated with a solid phase having a boronate reactive group to which glycated hemoglobin binds. Subsequently, or simultaneously, the sample mixture is reacted with a labeled anti-hemoglobin Ab. The resulting signal is detected and is directly proportional to the %GHb in the sample.
- the method according to the various embodiments of the invention thus eliminates the need for two measurements : one for GHb and another for total hemoglobin, with a ratio to then determine % GHb.
- glycated hemoglobin binds to the boronate affinity complex attached to the solid phase.
- Total hemoglobin may compete directly with glycated hemoglobin for binding to the solid phase and thereby permit the accurate determination of the per cent glycated hemoglobin in a single measurement.
- Whole blood can be hemolyzed in a variety of ways, either by diluting the whole blood sample in water, or, more preferably, using an agent such as a nonionic surfactant detergent, like TRITON ® X-100. Hemolysis releases hemoglobin and its derivatives from the red blood cells for analysis .
- an agent such as a nonionic surfactant detergent, like TRITON ® X-100. Hemolysis releases hemoglobin and its derivatives from the red blood cells for analysis .
- the GHb assay according to the present invention is based on the affinity of boronic, phenylboronic, boric acid and boronate etc. (hereinafter "boronate”) compounds or moieties for glycated hemoglobin. Boronate reacts with GHb in a sample through the cis-diol moiety of glucose bound to hemoglobin, forming a five-membered ring structure.
- a boronate group can be attached to a solid phase covalently, by a variety of chemistries, or electrostatically, and methods for doing so have been described in the art, including, for example, US Patent No. 5,459,080, incorporated herein by reference.
- the solid phase itself can be chosen from a variety of materials including, but not limited to beads, microparticles, magnetic microparticles, microtiter plates, tubes, and the like, made of polystyrene, polyacrylamide,- - agarose, dextran, latex, silica, glass, etc., which may be further derivitized to include functional surface groups.
- These functional surface groups include, but are not limited to aldehyde, aliphatic amine, aromatic amine, amide, carboxylic acid, sulfhydryl, chloromethyl, epoxy, hydrazide, hydroxyl, etc., which can then be covalently coupled to the boronate or boronate support following standard coupling techniques known to those skilled in the art.
- Preferred solid phases include amino functionalized magnetic latex particles and particularly preferred carboxylated magnetic latex particles, which have been derivatized to amino particles using a diamine and standard coupling techniques.
- the diamines include, but are not limited to, ethylenediamine, 1, 6-hexanediamine, 1,4- trans- cyclohexanediamine, with ethylenediamine being preferred.
- Preferred functional surface moieties for attachment of boronate compounds can be chosen from a variety of materials including, but not limited to, carboxymethylamylose, carboxymethylcellulose, polyaspartic acid, polyglutamic acid, polylysine, polyacrylic acid, proteins, albumins, antibodies, etc. and can be coupled via known techniques to the solid phase.
- Preferred functional surface moieties are carboxymethylcellulose, with carboxymethylamylose being particularly preferred.
- Boronate compounds for use with the method of the invention include those described in Gallop, US Patent No. 4,496,722, incorporated herein by reference.
- Preferred compounds include 4-carboxyphenylboronic acid, 3-nitro-5- carboxyphenylboronic acid and m-aminophenylboronic acid (APBA) .
- APBA m-aminophenylboronic acid
- Particularly preferred is m-aminophenylboronic acid (APBA) .
- the labeled component may be an antibody, and desirably may be a monoclonal or polyclonal antibody (Ab) , or Ab fragment containing the antigen binding site, or complementarity determining region (CDR) , such as an F(ab') 2 or Fab fragment .
- the detectable moiety or label may be a radioactive, fluorescent or chemiluminescent substance, or an enzyme.
- a labeled-second Ab which recognizes the species specific Fc fragment of the first Ab may also be used.
- the bound hemoglobin itself due to its peroxidase- like properties, can generate a detectable signal. This is accomplished by adding hydrogen peroxide, with or without addition of another substrate (e.g. isoluminol) .
- another substrate e.g. isoluminol
- whole blood samples are lysed and diluted using methods known in the art.
- the blood cells are lysed using agents available to the skilled artisan.
- surfactants and particularly nonionic surfactants, are preferred.
- a 1:80 dilution is typical, using for example, 0.5% TRITON ® X-100 nonionic surfactant.
- Azo- valeric initiated, carboxylated magnetic microparticles are coated with an amine, preferably ethylenediamine (EDA) , to which is attached a polymer, preferably a polymeric anion, and particularly carboxylic acid based polymeric anion, and a boronate compound.
- EDA ethylenediamine
- CMA carboxymethylamylose
- APBA l-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
- EDAC l-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
- RLU read-only indicator
- Other methods of reading the signal generated by the labeled component are also within the scope of the invention.
- Calibrators or standards that are run with the assay provide calibration (or standard) curves from which the %GHb in the sample is determined using the measured chemiluminescent signal. The measured chemiluminescent signal is directly proportional to the %GHb in the sample.
- %GHb was determined following hemolysis of whole blood to release hemoglobins, after which samples were diluted and incubated with a solid phase having a boronate reactive group to which glycated hemoglobin binds. Subsequently, or simultaneously, the sample mixture was reacted with a labeled anti-hemoglobin Ab. After washing, the resulting signal was detected and was directly proportional to the %GHb in the sample.
- CMA/MERQUAT ® coated amino microparticles Two ml of amino magnetic microparticles (#AM 40-500, Spherotech, Libertyville, IL) at 5% solids were washed 3 times with 10 ml of 50 mM 2- (N-Morpholino) ethanesulfonic acid, pH 6.2 (MES buffer) . The washed microparticles were then incubated in 10 ml of MES buffer containing 240 mg of carboxymethylamylose (CMA; #C4947, Sigma, St.
- CMA carboxymethylamylose
- EDAC l-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride
- the CMA/Merquat coated microparticles were used in conjunction with the IMx ® GHb Polyanion Reagent (Abbott Laboratories, Abbott Park, IL) as will be described below in Example 4.A. and 4.B.
- This polyanion reagent consists of phenylboronic acid coupled to polyacrylic acid.
- Microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg of m-aminophenylboronic acid (hemisulfate) (APBA) and 96 mg EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were washed 3 times with 100 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- APBA m-aminophenylboronic acid
- C. CMC-APBA coated amino microparticles Two ml of amino magnetic microparticles (#AM 40-500, Spherotech, Libertyville, IL) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 240 mg of carboxymethylcellulose (CMC) (MW 250,000; #41931-1, Aldrich, Milwaukee, WI) and 96 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were attracted to a magnet and the supernatant discarded.
- CMC carboxymethylcellulose
- Microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg of APBA and 96 mg EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were washed 3 times with 100 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- PAA-APBA coated amino microparticles Two ml of amino magnetic microparticles (#AM 40-500, Spherotech, Libertyville, IL) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 209 mg of 35% polyacrylic acid (PAA) (MW 250,000; #41600-2, Aldrich, Milwaukee, WI) and 96 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were attracted to a magnet and the supernatant discarded.
- PAA polyacrylic acid
- Microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg of APBA and 96 mg EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were washed 3 times with 100 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- TREN-CMA-APBA coated carboxyl microparticles Two ml of carboxyl magnetic microparticles (SP1267, Polymer Labs, Shropshire, UK) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 73 mg of Tris (2-aminoethyl) amine (TREN) and 96 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were attracted to a magnet and the supernatant discarded.
- TREN Tris (2-aminoethyl) amine
- Microparticles were washed 3 times with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 120 mg CMA mg and 48 mg EDAC for 60 minutes at room temperature on a rotator. After the second incubation, microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg APBA and 96 mg EDAC for another 60 minutes at room temperature on a rotator. After this final incubation, the microparticles were washed 3 times with 100 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- TREN-CMC-APBA coated carboxyl microparticles Two ml of carboxyl magnetic microparticles (SP1267, Polymer Labs, Shropshire, UK) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 73 mg of Tris (2- aminoethyl) amine (TREN) and 96 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were attracted to a magnet and the supernatant discarded.
- TREN Tris (2- aminoethyl) amine
- Microparticles were washed 3 times with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 120 mg CMC (MW 700,000; #41933-8, Aldrich, Milwaukee, WI) and 48 mg EDAC for 60 minutes at room temperature on a rotator. After the second incubation, microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg APBA and 96 mg EDAC for another 60 minutes at room temperature on a rotator. After this final incubation, the microparticles were washed 3 times with 100 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- G APBA coated carboxyl microparticles Two ml of carboxyl magnetic microparticles (SP1340, Polymer Labs, Shropshire, UK) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 43.2 mg of APBA and 96 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were washed twice with 10 ml of 50 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer. H.
- EDA-CMA-APBA coated carboxyl microparticles Two ml of azo-valeric initiated carboxylated magnetic microparticles (AB007C, Polymer Labs, Shropshire, UK) at 5% solids were washed 3 times with 10 ml of MES buffer. The washed microparticles were then incubated in 10 ml of MES buffer containing 15 1 of ethylenediamine (EDA) and 10 mg of EDAC for 60 minutes at room temperature on a rotator. After incubation, the microparticles were attracted to a magnet and the supernatant discarded.
- EDA ethylenediamine
- Microparticles were washed 3 times with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 120 mg CMA mg and 19.6 mg EDAC for 60 minutes at room temperature on a rotator. After the second incubation, microparticles were washed once with 10 ml of MES buffer, then incubated in 10 ml of MES buffer containing 46.5 mg APBA and 96 mg EDAC for another 60 minutes at room temperature on a rotator. After this final incubation, the microparticles were washed 3 times with 50 mM taurine buffer, pH 9.0, and resuspended in 10 ml of the same buffer.
- Example 2 Antibody Conjugation with Acridinium Antibody to be conjugated was first dialyzed against 3 changes of phosphate buffered saline (PBS) , utilizing 4 to 6 hours per dialysis change. Antibody was then diluted to a concentration of 1 mg/ml. While mixing the 1 mg/ml antibody solution, 10% 3- [ (3-cholamidopropyl) -dimethyammonio] -1- propane sulfonate (CHAPS) and 5 ug/ml sulfopropyl acridinium ester was added. This was mixed for 10 minutes at room temperature then loaded onto a 120 ml bed volume (1.6 x 60 cm) Pharmacia Sephacryl S-200 column.
- PBS phosphate buffered saline
- the column buffer used was 2.28 mM sodium phosphate monobasic, 7.68 mM sodium phosphate dibasic, 145 mM NaCl, 0.1 % CHAPS, pH 6.3. One ml fractions were collected, and those fractions with an absorbance at 280 nm greater than or equal to 0.1 were pooled.
- the acridinium- labeled antibody was diluted to a final concentration of 40 ng/ml in Conjugate Diluent (10 mM MES, 150 mM NaCl, pH 6.3 containing 2% bovine serum albumen " (BSA) and 0.5% TRITON ® X-100) .
- Common Buffer 4 mM sodium phosphate, 150 mM NaCl, pH 7.5 containing 0.05% Brij and 0.1 % NaN 3
- Chemiluminescent signal was generated by adding Trigger Reagents of 0.053% HN0 3 , 1.2% H 2 0 2 and 0.9%
- DPTA Diethylenetriaminepentaacetic acid
- RLU Relative Light Unit
- CMA/Merquat coated particles Sample + Polyanion Reagent Whole blood samples were lysed by diluting them 1:5 in distilled water. The hemolysate was then further diluted 1:16 in 0.5% TETRONIC ® 1307 (BASF #550193, Mount Olive, NJ) in PBS. Microparticles prepared in Example l.A. were washed with 100 mM taurine buffer, pH 9.0, then resuspended in the same taurine buffer at 0.1% solids.
- the diluted whole blood samples were mixed with an equal volume of the IMx ® GHb Polyanion Reagent (Abbott Laboratories, Abbott Park, IL) and incubated at 37°C for 7 minutes to allow the GHb in the sample to bind to the boronate group of the Polyanion Reagent. After incubation, 50 1 of this mixture was mixed with 50 1 of microparticles, incubated and washed as in Example 3.
- the IMx ® GHb Polyanion Reagent Abbott Laboratories, Abbott Park, IL
- Example 2 A DEAE-purified mouse monoclonal antibody (IgG 1 )to human hemoglobin (clone MIH 9505, Medix Biotech, Inc., San Carlos, CA) which had been labeled with acridinium as in Example 2. , was added to the washed microparticles and the experiment completed as in Example 3.
- CMA/Merquat coated particles + Polyanion Reagent The CMA/Merquat coated microparticles from Example l.A. were overcoated with the IMx ® GHb Polyanion Reagent (Abbott Laboratories, Abbott Park, IL) by washing the microparticles with 100 mM taurine buffer, pH 9.0 and resuspending them in the Polyanion Reagent at 0.1% solids. Microparticles were then washed with 100 mM taurine buffer, pH 9.0 to remove excess Polyanion Reagent, and resuspended in the taurine buffer at 0.1% solids.
- IMx ® GHb Polyanion Reagent Abbott Laboratories, Abbott Park, IL
- Example 4.A Fifty microliters of sample hemolysate, prepared as in Example 4.A., was then mixed with 50 1 of microparticles and the GHb assay was performed as described in Example 3 using the same antibody as in Example 4.A.
- Example 4.A Whole blood samples were selected as in Example 4.A. and hemolyzed by diluting 1:80 in 0.5 % TRITON ® X-100 in distilled water. The GHb assay was then performed as in Example 3. using the microparticles from Example l.B. and the same antibody as in Example 4. .
- Example 3 CMC-APBA coated particles
- the GHb assay was performed as in Example 3. using samples prepared as in Example 4.C., the microparticles from Example l.C. and the same antibody as in Example 4.A.
- Example 5 Correlation of IPLS GHb assay with Biorad Diamat HPLC and Abbott IMx ® GHb 110 whole blood samples were obtained from a hospital lab, where previously they had been tested for GHb using the Biorad Diamat HPLC method to obtain %HbAlc levels.
- Boronate affinity binding methods such as are used in the assays described here, detect all GHb species, including HbAlc. Since there is a linear relationship between GHb and HbAlc, comparisons can be made between tests measuring GHb and HbAlc .
- IPLS GHb assay was performed as in Example 4.1. with the 110 samples prepared as in Example 4.C. Results were correlated with the Biorad Diamat HPLC (Brea, CA) and IMx ® GHb (Abbott Laboratories, Abbott Park, IL) assays ( Figures 9 and 10 respectively) .
- Example 6 One Step Format
- the hemolysate samples were prepared as in Example 4.C.
- Microparticles, prepared in Example l.H., were washed with 50 mM taurine buffer, pH 9.0 and resuspended in the same buffer at 0.1% solids.
- the labeled antibody was the same antibody as in Example 4.A.
- the assay was performed by incubating 50 1 of hemolysate, 50 1 of microparticles and 50 1 of acridinium-labeled antibody at 37°C for 25 minutes. The particles were then attracted to a magnet and washed 4 times with 1 ml of Common Buffer.
- Chemiluminescent signal was generated by adding Trigger Reagents.
- RLU chemiluminescent signal
- Example 7 Use of Different Antibodies
- a DEAE-purified mouse monoclonal antibody IgG to human hemoglobin from Medix Biotech.
- Other antibodies including polyclonal, from different species, and monoclonal, can also be used.
- Example 3 The GHb assay was performed as in Example 3. except both incubations were done at 37°C for 10 minutes, with samples prepared as in Example 4.A., the microparticles from Example l.A. and the following antibodies to human hemoglobin labeled with acridinium as in Example 2: (1) polyclonal goat anti-human hemoglobin (#CR8000 GAP, Cortex Biochem, San Leandro, CA) , (2) monoclonal anti-human hemoglobin (#A36380, BiosPacific, Emeryville, CA) , (3) polyclonal goat anti-human hemoglobin (#G32100, Bios Pacific, Emeryville, CA) , and (4) polyclonal rabbit anti-human hemoglobin (#A118, Dako Corp., Carpinteria, CA) .
- polyclonal goat anti-human hemoglobin #CR8000 GAP, Cortex Biochem, San Leandro, CA
- monoclonal anti-human hemoglobin #A36380, BiosPacific, Emeryville
- results show that the chemiluminescent signal (RLU) was directly proportional to the %GHb in the sample.
- the correlation coefficients (R values) for assays using each Ab were 0.982, 0.975, 0.946 and 0.869 respectively.
- polyclonal or monoclonal antibodies to human hemoglobin are useable in this assay format for accurately detecting %GHb.
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Abstract
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AT98910389T ATE249049T1 (de) | 1997-03-13 | 1998-03-13 | Verfahren zur bestimmung des prozentualen anteils von glykosiliertem hämoglobin |
JP53986898A JP3356785B2 (ja) | 1997-03-13 | 1998-03-13 | グリコヘモグロビン(%)の定量 |
DE69817794T DE69817794T2 (de) | 1997-03-13 | 1998-03-13 | Verfahren zur bestimmung des prozentualen anteils von glykosiliertem hämoglobin |
EP98910389A EP0974060B1 (fr) | 1997-03-13 | 1998-03-13 | Determination du pourcentage d'hemoglobine glycosylee |
CA002282586A CA2282586A1 (fr) | 1997-03-13 | 1998-03-13 | Determination du pourcentage d'hemoglobine glycosylee |
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US08/816,237 US6162645A (en) | 1997-03-13 | 1997-03-13 | Determination of % glycated hemoglobin |
US08/816,237 | 1997-03-13 |
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CA (1) | CA2282586A1 (fr) |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4269605A (en) * | 1978-06-28 | 1981-05-26 | Amicon Corporation | Method and kit for separation of glycoproteins |
US4861728A (en) * | 1987-07-24 | 1989-08-29 | Becton, Dickinson And Company | Immunoassay of glycosylated hemoglobin using a labeled boron reagent |
WO1990013818A1 (fr) * | 1989-05-11 | 1990-11-15 | Axis Research As | PROCEDE DE MESURE DE L'HEMOGLOBINE GLUCOSYlEE |
EP0455225A2 (fr) * | 1990-05-01 | 1991-11-06 | Nacalai Tesque, Inc. | Méthode pour mesurer le pourcentage glycation d'une protéine particulière |
WO1992022818A1 (fr) * | 1991-06-19 | 1992-12-23 | Abbott Laboratories | Mesure rapide de l'hemoglobine glycosylee |
WO1993018407A1 (fr) * | 1992-03-04 | 1993-09-16 | Abbott Laboratories | Determination de l'hemoglobine glycosylee par reduction de l'intensite de la fluorescence |
EP0708337A2 (fr) * | 1994-10-19 | 1996-04-24 | Fuji Photo Film Co., Ltd. | Elément analytique et méthode d'analyse de l'hémoglobine glycolysée |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4496722A (en) * | 1981-05-01 | 1985-01-29 | The Children's Medical Center Corporation | Reporter compounds |
US4727036A (en) * | 1985-08-08 | 1988-02-23 | Molecular Diagnostics, Inc. | Antibodies for use in determining hemoglobin A1c |
US5206144A (en) * | 1985-03-29 | 1993-04-27 | Novo Industri A/S | Determination of glycated (glycosylated) hemoglobin in blood |
GB2181840B (en) * | 1985-10-16 | 1989-11-29 | Farmos Group Ltd | Method for the immunoassay of a macromolecular analyte |
JPS637558A (ja) * | 1986-06-27 | 1988-01-13 | Sony Corp | デイスクプレ−ヤ |
DE3750503T2 (de) * | 1986-10-22 | 1995-02-09 | Abbott Lab | Chemilumineszierende Acridinium- und Phenantridiniumsalze. |
IT1206003B (it) * | 1987-05-18 | 1989-04-05 | Menarini Sas | Soluzione di controllo della funzio nalita' della colonna cromatografica a scambio ionico di apparecchiature hplc (cromatografia liquida ad alta prestazione), e relativo procedimento di preparazione |
EP0446285A4 (en) * | 1988-12-05 | 1993-03-10 | Primus Corporation | Method for determination of glycated proteinaceous species |
US5110745A (en) * | 1989-06-01 | 1992-05-05 | The Trustees Of The University Of Pennsylvania | Methods of detecting glycated proteins |
JP2619537B2 (ja) * | 1989-09-18 | 1997-06-11 | 株式会社日立製作所 | 液体クロマトグラフの方法,その装置,そのシステム,及びその分離カラム |
JP2855533B2 (ja) * | 1990-02-22 | 1999-02-10 | 磐田化学工業株式会社 | 飲食品用酸味料 |
JP2782470B2 (ja) * | 1990-07-20 | 1998-07-30 | 株式会社日立製作所 | グリコヘモグロビンの分離方法および分離装置並びに分離カラム |
JP2929462B2 (ja) * | 1990-08-29 | 1999-08-03 | 東ソー株式会社 | 安定型糖化ヘモグロビン分析方法 |
JPH04145367A (ja) * | 1990-10-06 | 1992-05-19 | Masashi Funayama | ヘモグロビンA1c(HbA1c)の分画定量方法と、それを実施する為に構成されたヘモグロビンA1c(HbA1c)定量キット |
GB9024771D0 (en) * | 1990-11-14 | 1991-01-02 | Axis Research | Assay |
US5284777A (en) * | 1991-03-04 | 1994-02-08 | Isolab, Inc. | Combined glycated hemoglobin and immunoturbidometric glycated albumin assay from whole blood lysate |
US5506114A (en) * | 1992-02-07 | 1996-04-09 | Osborn Laboratories | Methods and kits for detecting the presence or concentration of biological analytes |
US5470759A (en) * | 1992-06-10 | 1995-11-28 | Fujirebio, Inc. | Anti-glycated hemoglobin monoclonal antibody and method for measuring glycated hemoglobin |
-
1997
- 1997-03-13 US US08/816,237 patent/US6162645A/en not_active Expired - Lifetime
-
1998
- 1998-03-13 DE DE69817794T patent/DE69817794T2/de not_active Expired - Fee Related
- 1998-03-13 ES ES98910389T patent/ES2206900T3/es not_active Expired - Lifetime
- 1998-03-13 WO PCT/US1998/005005 patent/WO1998040750A1/fr active IP Right Grant
- 1998-03-13 JP JP53986898A patent/JP3356785B2/ja not_active Expired - Fee Related
- 1998-03-13 EP EP98910389A patent/EP0974060B1/fr not_active Expired - Lifetime
- 1998-03-13 CA CA002282586A patent/CA2282586A1/fr not_active Abandoned
- 1998-03-13 AT AT98910389T patent/ATE249049T1/de not_active IP Right Cessation
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4269605A (en) * | 1978-06-28 | 1981-05-26 | Amicon Corporation | Method and kit for separation of glycoproteins |
US4861728A (en) * | 1987-07-24 | 1989-08-29 | Becton, Dickinson And Company | Immunoassay of glycosylated hemoglobin using a labeled boron reagent |
WO1990013818A1 (fr) * | 1989-05-11 | 1990-11-15 | Axis Research As | PROCEDE DE MESURE DE L'HEMOGLOBINE GLUCOSYlEE |
EP0455225A2 (fr) * | 1990-05-01 | 1991-11-06 | Nacalai Tesque, Inc. | Méthode pour mesurer le pourcentage glycation d'une protéine particulière |
WO1992022818A1 (fr) * | 1991-06-19 | 1992-12-23 | Abbott Laboratories | Mesure rapide de l'hemoglobine glycosylee |
WO1993018407A1 (fr) * | 1992-03-04 | 1993-09-16 | Abbott Laboratories | Determination de l'hemoglobine glycosylee par reduction de l'intensite de la fluorescence |
EP0708337A2 (fr) * | 1994-10-19 | 1996-04-24 | Fuji Photo Film Co., Ltd. | Elément analytique et méthode d'analyse de l'hémoglobine glycolysée |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6316265B1 (en) | 1997-03-13 | 2001-11-13 | Abbott Laboratories | Determination of % glycated hemoglobin |
WO2001020338A1 (fr) * | 1999-09-17 | 2001-03-22 | Abbott Laboratories | Determination du pourcentage d'hemoglobine glyquee |
JP2003509693A (ja) * | 1999-09-17 | 2003-03-11 | アボット・ラボラトリーズ | グリコヘモグロビン(%)の定量 |
WO2002061436A1 (fr) * | 2001-01-31 | 2002-08-08 | Scripps Laboratories | Procedes et dispositifs de quantification de proteine glyquee |
US7195923B2 (en) | 2001-01-31 | 2007-03-27 | Scripps Laboratories, Inc. | Ratiometric determination of glycated protein |
US7695973B2 (en) | 2001-01-31 | 2010-04-13 | Scripps Laboratories, Inc. | Determination of glycated protein |
WO2010093482A1 (fr) * | 2009-01-14 | 2010-08-19 | Abbott Laboratories | Méthodes et trousses de détection d'hémoglobine dans des échantillons d'essai |
US8241915B2 (en) | 2009-01-14 | 2012-08-14 | Abbott Laboratories | Methods and kits for detecting hemoglobin in test samples |
FR2947632A1 (fr) * | 2009-07-01 | 2011-01-07 | Sebia Sa | Analyse et dosage d'hemoglobines glyquees par electrophorese capillaire, compositions tampon et kits pour electrophorese capillaire |
WO2011001045A1 (fr) * | 2009-07-01 | 2011-01-06 | Sebia | Analyse et dosage d'hémoglobines glyquées par électrophorèse capillaire, compositions tampon et kits pour électrophorèse capillaire |
US9222913B2 (en) | 2009-07-01 | 2015-12-29 | Sebia | Analysis and assay of glycated haemoglobins by capillary electrophoresis, buffer compositions and kits for capillary electrophoresis |
US10119933B2 (en) | 2009-07-01 | 2018-11-06 | Sebia | Analysis and assay of glycated haemoglobins by capillary electrophoresis, buffer compositions and kits for capillary electrophoresis |
US9624522B2 (en) | 2009-08-07 | 2017-04-18 | Ohmx Corporation | Single, direct detection of hemoglobin A1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay |
EP2765425A1 (fr) * | 2013-02-07 | 2014-08-13 | Samsung Electronics Co., Ltd | Support pour capturer la protéine glyquée dans un échantillon et dispositif et procédé de mesure de la protéine glyquée utilisant ledit support |
EP3404413A1 (fr) | 2014-03-20 | 2018-11-21 | Bio-Rad Laboratories, Inc. | Dosage de protéines glycatées |
WO2020180335A1 (fr) | 2019-03-05 | 2020-09-10 | Victor Manneh | Dosage quotientométrique par liaison de saturation |
US11802881B2 (en) | 2019-03-05 | 2023-10-31 | Lumiradx Uk Ltd. | Saturation binding ratiometric assay |
Also Published As
Publication number | Publication date |
---|---|
EP0974060A1 (fr) | 2000-01-26 |
ES2206900T3 (es) | 2004-05-16 |
US6162645A (en) | 2000-12-19 |
EP0974060B1 (fr) | 2003-09-03 |
JP2000514196A (ja) | 2000-10-24 |
DE69817794D1 (de) | 2003-10-09 |
JP3356785B2 (ja) | 2002-12-16 |
DE69817794T2 (de) | 2004-08-05 |
CA2282586A1 (fr) | 1998-09-17 |
ATE249049T1 (de) | 2003-09-15 |
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